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The map label for this gene is ilvE [H]

Identifier: 148828343

GI number: 148828343

Start: 1786947

End: 1787978

Strand: Reverse

Name: ilvE [H]

Synonym: CGSHiGG_09675

Alternate gene names: 148828343

Gene position: 1787978-1786947 (Counterclockwise)

Preceding gene: 148828347

Following gene: 148828341

Centisome position: 94.74

GC content: 43.41

Gene sequence:

>1032_bases
ATGAAAGACTTAGACTGGAATAACCTTGGTTTTAGTTATATTAAAACAGATTACCGCTTCATCGCTCACTGGAAAGATGG
CAAATGGGACGAAGGCAAACTCACCACAGACAATACTTTACATATTCACGAAGGTTCTACCGCACTTCACTACGGCCAAC
AATGCTTTGAAGGCTTAAAGGCTTACCGTTGCAAAGATGGTTCAATCAACTTATTCCGCCCGCAAGCAAATGCAGAGCGT
ATGCAACGTACCGCAGACCGTCTTTTAATGCCACGCGTACCAACTGAATTATTCGTGCGTGCATGTAAAAAAGTGGTTAA
AGCTAACCAAGATTGGTTGGGTCCTTACGGTTCTGGTGCAACCTTATACTTACGTCCATTCTTAATTGGCGTGGGCGAAA
ATATCGGCGTGAAAACGGCACCAGAGTTTATTTTCTCTGTTTTTTGTTGCCCAGTGGGTGCTTACTTCAAAGGTGGTTTG
GCTCCATCAAACTTTATCACGACTGATTATGACCGTGCTGCACCAATGGGCACTGGTGGTGTGAAAGTGGGTGGGAACTA
CGCAGCAAGCTTACTTCCACACGAATTAGTCGCAGAACAAGGTACGCCAGAACGTAAATTTGCGGATGCAATCTATTTAG
ATCCAAAAACTCACACCAAAATTGAAGAAGTCGGTGCGGCAAACTTCTTTGGGATCACAAAAGACAATAAATTCATCACA
CCAAAATCAGAATCTATTTTACCAAGTATCACTAAATACTCTCTTTTACATATCGCAAAAGAGCGTTTAGGTATGGAAGC
TATTGAAGGCGATGTGTATATCGATCAACTCGATCAATTCGTAGAAGCTGGTGCGTGCGGTACAGCGGCGGTGATTACCC
CAGTGGGCGGCATTCAACACAACGGAAAATTCCACGTATTCTATTCAGAAACTGAAGTAGGCCCTGTTACCCGTCGTCTT
TATGATGAATTAACAGGCATCCAATTCGGTGATGTTGAAGCACCTGAAGGCTGGATTGTGAAAGTAGAATAA

Upstream 100 bases:

>100_bases
CTAAAAATTGCATCATTTCGAGAATTTATTATTTAAATCACTTCAGGAATGGTGTAGATTAAGCGAAATTTCATTCAAAA
AATAATATTAAGGAAGTAAA

Downstream 100 bases:

>100_bases
TTTCTACCAATAGAAAAACGAAAGTGCGGTTAAAAATCAACCGCACTTTTTTATTGATTCAATCCCCATTGGCGAGAATC
TTTATCAAATTTAACACCTG

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT [H]

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MKDLDWNNLGFSYIKTDYRFIAHWKDGKWDEGKLTTDNTLHIHEGSTALHYGQQCFEGLKAYRCKDGSINLFRPQANAER
MQRTADRLLMPRVPTELFVRACKKVVKANQDWLGPYGSGATLYLRPFLIGVGENIGVKTAPEFIFSVFCCPVGAYFKGGL
APSNFITTDYDRAAPMGTGGVKVGGNYAASLLPHELVAEQGTPERKFADAIYLDPKTHTKIEEVGAANFFGITKDNKFIT
PKSESILPSITKYSLLHIAKERLGMEAIEGDVYIDQLDQFVEAGACGTAAVITPVGGIQHNGKFHVFYSETEVGPVTRRL
YDELTGIQFGDVEAPEGWIVKVE

Sequences:

>Translated_343_residues
MKDLDWNNLGFSYIKTDYRFIAHWKDGKWDEGKLTTDNTLHIHEGSTALHYGQQCFEGLKAYRCKDGSINLFRPQANAER
MQRTADRLLMPRVPTELFVRACKKVVKANQDWLGPYGSGATLYLRPFLIGVGENIGVKTAPEFIFSVFCCPVGAYFKGGL
APSNFITTDYDRAAPMGTGGVKVGGNYAASLLPHELVAEQGTPERKFADAIYLDPKTHTKIEEVGAANFFGITKDNKFIT
PKSESILPSITKYSLLHIAKERLGMEAIEGDVYIDQLDQFVEAGACGTAAVITPVGGIQHNGKFHVFYSETEVGPVTRRL
YDELTGIQFGDVEAPEGWIVKVE
>Mature_343_residues
MKDLDWNNLGFSYIKTDYRFIAHWKDGKWDEGKLTTDNTLHIHEGSTALHYGQQCFEGLKAYRCKDGSINLFRPQANAER
MQRTADRLLMPRVPTELFVRACKKVVKANQDWLGPYGSGATLYLRPFLIGVGENIGVKTAPEFIFSVFCCPVGAYFKGGL
APSNFITTDYDRAAPMGTGGVKVGGNYAASLLPHELVAEQGTPERKFADAIYLDPKTHTKIEEVGAANFFGITKDNKFIT
PKSESILPSITKYSLLHIAKERLGMEAIEGDVYIDQLDQFVEAGACGTAAVITPVGGIQHNGKFHVFYSETEVGPVTRRL
YDELTGIQFGDVEAPEGWIVKVE

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI38176287, Length=345, Percent_Identity=33.3333333333333, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI296010906, Length=345, Percent_Identity=33.3333333333333, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI296010904, Length=345, Percent_Identity=33.3333333333333, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI50658084, Length=336, Percent_Identity=31.25, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI258614015, Length=291, Percent_Identity=31.6151202749141, Blast_Score=131, Evalue=8e-31,
Organism=Homo sapiens, GI296010902, Length=345, Percent_Identity=28.1159420289855, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI296010900, Length=345, Percent_Identity=28.1159420289855, Blast_Score=113, Evalue=2e-25,
Organism=Escherichia coli, GI48994963, Length=328, Percent_Identity=29.2682926829268, Blast_Score=107, Evalue=9e-25,
Organism=Caenorhabditis elegans, GI17568601, Length=358, Percent_Identity=31.0055865921788, Blast_Score=163, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI17565728, Length=348, Percent_Identity=29.3103448275862, Blast_Score=117, Evalue=9e-27,
Organism=Saccharomyces cerevisiae, GI6322608, Length=337, Percent_Identity=32.0474777448071, Blast_Score=164, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6322002, Length=345, Percent_Identity=30.7246376811594, Blast_Score=147, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24641779, Length=350, Percent_Identity=30.2857142857143, Blast_Score=160, Evalue=1e-39,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005786 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 37989; Mature: 37989

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDLDWNNLGFSYIKTDYRFIAHWKDGKWDEGKLTTDNTLHIHEGSTALHYGQQCFEGLK
CCCCCCCCCCHHHEECCEEEEEEECCCCCCCCEECCCCEEEEECCCHHHHHHHHHHHHHH
AYRCKDGSINLFRPQANAERMQRTADRLLMPRVPTELFVRACKKVVKANQDWLGPYGSGA
HEECCCCCEEEECCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCC
TLYLRPFLIGVGENIGVKTAPEFIFSVFCCPVGAYFKGGLAPSNFITTDYDRAAPMGTGG
EEEEEEEEEECCCCCCCCHHHHHHHHHHHHCCHHHHCCCCCCCCCEECCCCCCCCCCCCC
VKVGGNYAASLLPHELVAEQGTPERKFADAIYLDPKTHTKIEEVGAANFFGITKDNKFIT
EEECCCHHHHHCHHHHHHCCCCCCHHHCCEEEECCCCCCHHHHCCCCCEEEECCCCEEEC
PKSESILPSITKYSLLHIAKERLGMEAIEGDVYIDQLDQFVEAGACGTAAVITPVGGIQH
CCCCCCCCHHHHHHHHHHHHHHHCHHHHCCCEEHHHHHHHHHCCCCCCEEEEECCCCCEE
NGKFHVFYSETEVGPVTRRLYDELTGIQFGDVEAPEGWIVKVE
CCEEEEEECCCCCCHHHHHHHHHHHCCEECCCCCCCCCEEEEC
>Mature Secondary Structure
MKDLDWNNLGFSYIKTDYRFIAHWKDGKWDEGKLTTDNTLHIHEGSTALHYGQQCFEGLK
CCCCCCCCCCHHHEECCEEEEEEECCCCCCCCEECCCCEEEEECCCHHHHHHHHHHHHHH
AYRCKDGSINLFRPQANAERMQRTADRLLMPRVPTELFVRACKKVVKANQDWLGPYGSGA
HEECCCCCEEEECCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCC
TLYLRPFLIGVGENIGVKTAPEFIFSVFCCPVGAYFKGGLAPSNFITTDYDRAAPMGTGG
EEEEEEEEEECCCCCCCCHHHHHHHHHHHHCCHHHHCCCCCCCCCEECCCCCCCCCCCCC
VKVGGNYAASLLPHELVAEQGTPERKFADAIYLDPKTHTKIEEVGAANFFGITKDNKFIT
EEECCCHHHHHCHHHHHHCCCCCCHHHCCEEEECCCCCCHHHHCCCCCEEEECCCCEEEC
PKSESILPSITKYSLLHIAKERLGMEAIEGDVYIDQLDQFVEAGACGTAAVITPVGGIQH
CCCCCCCCHHHHHHHHHHHHHHHCHHHHCCCEEHHHHHHHHHCCCCCCEEEEECCCCCEE
NGKFHVFYSETEVGPVTRRLYDELTGIQFGDVEAPEGWIVKVE
CCEEEEEECCCCCCHHHHHHHHHHHCCEECCCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]