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The map label for this gene is metK [H]

Identifier: 148828326

GI number: 148828326

Start: 1770370

End: 1771524

Strand: Direct

Name: metK [H]

Synonym: CGSHiGG_09560

Alternate gene names: 148828326

Gene position: 1770370-1771524 (Clockwise)

Preceding gene: 148828325

Following gene: 148828327

Centisome position: 93.81

GC content: 39.74

Gene sequence:

>1155_bases
ATGTCTAGTTATTTATTTACATCTGAATCAGTCTCTGAAGGGCATCCAGATAAAATAGCCGATCAAATTTCTGATGCAGT
ACTTGATGAAATCCTAAAACAAGATCCAAAAGCACGAGTAGCTTGTGAAACTTACGTAAAAACCGGTATGGCATTGGTGG
GCGGGGAAATCACCACATCAGCATGGGTCGATATTGAGAATTTAACCCGTAAAGTGATTTGTGATATTGGTTATGAACAT
TCTGAAATGGGTTTTGATGGTCATTCTTGTGCGGTGCTTAATGCGATTGGTAAACAATCTGCAGATATTAATCAAGGCGT
TGATCGTGAAAATCCATTAGATCAAGGCGCAGGCGACCAAGGTATTATGTTTGGTTATGCCACCAATGAAACGGATGTAT
TAATGCCTGCAGCTATTACTTATGCACATCGTTTAATGGAAAAACAAGCTGAAGTGCGTAAAAGCGGTAAATTAGCATGG
TTACGCCCAGATGCGAAAAGCCAAGTTACTTTAAAATATGAAGATAATAAAATTGTTGGTGTGGATGCGGTTGTGCTTTC
TACTCAACATTCTGAAGAAGTTAGCCAAAAAGATTTACACGAAGGTGTGATGGAAGAAATTATCAAACCTGTATTACCAA
GTGAATGGCTTTCTAAAGAAACAAAATTCTTCATTAACCCGACTGGTCGTTTTGTTATCGGTGGGCCAATGGGCGATTGT
GGTTTAACTGGTCGTAAAATTATCGTGGATACTTACGGTGGTGCTGCTCGTCACGGTGGTGGCGCATTCTCAGGAAAAGA
TCCATCTAAAGTAGATCGTTCTGCGGCTTATGCTGCACGTTATGTCGCAAAAAATATTGTGGCAGCTGGTTTAGCAGATC
GTTGTGAAATCCAACTTTCTTATGCGATTGGTGTGGCTGATCCAACATCTATCATGGTCGAAACCTTTGGAACTGGAAAA
GTTGCAAATGAATTATTAGTGTCATTAGTTCGTGAGTTTTTTGATTTACGTCCTTATGGCTTAATTAAAATGTTAGATTT
AATCCAACCAATCTATCGTGAAACTGCAGCTTATGGCCATTTCGGTCGTGAGCAATTCCCTTGGGAAAAAGTGGATCGAG
CAGAAGATTTACGTGTTGCGGCAGGTTTAAAATAA

Upstream 100 bases:

>100_bases
CACAACTACCCCATAATACCGCTTGCTTATTAAGCAAAAATGCGTATTATAGCCGTCTATACGTCAATACATCTAAATTT
AGAAGAAAAAGAGAGAAAAT

Downstream 100 bases:

>100_bases
TAGGCTTGAATTTTTAGTTTGAAAGGCTTATATTGGAAAGGCAACAAGGTTTCTTGTTGCCTTAGTTGGTTTAGGTTGCT
ACCAAGCTGGGGAGCTAAAG

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 384; Mature: 383

Protein sequence:

>384_residues
MSSYLFTSESVSEGHPDKIADQISDAVLDEILKQDPKARVACETYVKTGMALVGGEITTSAWVDIENLTRKVICDIGYEH
SEMGFDGHSCAVLNAIGKQSADINQGVDRENPLDQGAGDQGIMFGYATNETDVLMPAAITYAHRLMEKQAEVRKSGKLAW
LRPDAKSQVTLKYEDNKIVGVDAVVLSTQHSEEVSQKDLHEGVMEEIIKPVLPSEWLSKETKFFINPTGRFVIGGPMGDC
GLTGRKIIVDTYGGAARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQLSYAIGVADPTSIMVETFGTGK
VANELLVSLVREFFDLRPYGLIKMLDLIQPIYRETAAYGHFGREQFPWEKVDRAEDLRVAAGLK

Sequences:

>Translated_384_residues
MSSYLFTSESVSEGHPDKIADQISDAVLDEILKQDPKARVACETYVKTGMALVGGEITTSAWVDIENLTRKVICDIGYEH
SEMGFDGHSCAVLNAIGKQSADINQGVDRENPLDQGAGDQGIMFGYATNETDVLMPAAITYAHRLMEKQAEVRKSGKLAW
LRPDAKSQVTLKYEDNKIVGVDAVVLSTQHSEEVSQKDLHEGVMEEIIKPVLPSEWLSKETKFFINPTGRFVIGGPMGDC
GLTGRKIIVDTYGGAARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQLSYAIGVADPTSIMVETFGTGK
VANELLVSLVREFFDLRPYGLIKMLDLIQPIYRETAAYGHFGREQFPWEKVDRAEDLRVAAGLK
>Mature_383_residues
SSYLFTSESVSEGHPDKIADQISDAVLDEILKQDPKARVACETYVKTGMALVGGEITTSAWVDIENLTRKVICDIGYEHS
EMGFDGHSCAVLNAIGKQSADINQGVDRENPLDQGAGDQGIMFGYATNETDVLMPAAITYAHRLMEKQAEVRKSGKLAWL
RPDAKSQVTLKYEDNKIVGVDAVVLSTQHSEEVSQKDLHEGVMEEIIKPVLPSEWLSKETKFFINPTGRFVIGGPMGDCG
LTGRKIIVDTYGGAARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQLSYAIGVADPTSIMVETFGTGKV
ANELLVSLVREFFDLRPYGLIKMLDLIQPIYRETAAYGHFGREQFPWEKVDRAEDLRVAAGLK

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI5174529, Length=373, Percent_Identity=56.3002680965147, Blast_Score=405, Evalue=1e-113,
Organism=Homo sapiens, GI4557737, Length=372, Percent_Identity=56.1827956989247, Blast_Score=404, Evalue=1e-112,
Organism=Escherichia coli, GI1789311, Length=384, Percent_Identity=83.3333333333333, Blast_Score=676, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17538498, Length=386, Percent_Identity=55.9585492227979, Blast_Score=411, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI17551082, Length=374, Percent_Identity=55.8823529411765, Blast_Score=405, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI17538494, Length=373, Percent_Identity=56.8364611260054, Blast_Score=404, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI25145633, Length=385, Percent_Identity=55.5844155844156, Blast_Score=404, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI32565905, Length=373, Percent_Identity=50.9383378016086, Blast_Score=344, Evalue=6e-95,
Organism=Caenorhabditis elegans, GI32565911, Length=340, Percent_Identity=52.9411764705882, Blast_Score=337, Evalue=5e-93,
Organism=Caenorhabditis elegans, GI25145635, Length=337, Percent_Identity=52.8189910979229, Blast_Score=330, Evalue=1e-90,
Organism=Caenorhabditis elegans, GI32565903, Length=303, Percent_Identity=53.4653465346535, Blast_Score=302, Evalue=2e-82,
Organism=Caenorhabditis elegans, GI32565909, Length=84, Percent_Identity=63.0952380952381, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI32565907, Length=45, Percent_Identity=71.1111111111111, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323209, Length=373, Percent_Identity=56.8364611260054, Blast_Score=412, Evalue=1e-116,
Organism=Saccharomyces cerevisiae, GI6320710, Length=378, Percent_Identity=55.5555555555556, Blast_Score=410, Evalue=1e-115,
Organism=Drosophila melanogaster, GI45552159, Length=379, Percent_Identity=54.8812664907652, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580529, Length=379, Percent_Identity=54.8812664907652, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580537, Length=379, Percent_Identity=54.8812664907652, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580533, Length=379, Percent_Identity=54.8812664907652, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580535, Length=379, Percent_Identity=54.8812664907652, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580531, Length=379, Percent_Identity=54.8812664907652, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580527, Length=380, Percent_Identity=55.2631578947368, Blast_Score=380, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580525, Length=380, Percent_Identity=55.2631578947368, Blast_Score=380, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580541, Length=374, Percent_Identity=53.7433155080214, Blast_Score=356, Evalue=2e-98,
Organism=Drosophila melanogaster, GI24580539, Length=121, Percent_Identity=56.198347107438, Blast_Score=125, Evalue=4e-29,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 41916; Mature: 41785

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSYLFTSESVSEGHPDKIADQISDAVLDEILKQDPKARVACETYVKTGMALVGGEITTS
CCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCHHHCCCCCCHH
AWVDIENLTRKVICDIGYEHSEMGFDGHSCAVLNAIGKQSADINQGVDRENPLDQGAGDQ
HEEEHHHHHHHHHHHCCCCHHHCCCCCCCHHHHHHHCCCCCCHHCCCCCCCCCCCCCCCC
GIMFGYATNETDVLMPAAITYAHRLMEKQAEVRKSGKLAWLRPDAKSQVTLKYEDNKIVG
CEEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCEEEEEECCCEEEE
VDAVVLSTQHSEEVSQKDLHEGVMEEIIKPVLPSEWLSKETKFFINPTGRFVIGGPMGDC
EEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCEEEECCCCCEEECCCCCCC
GLTGRKIIVDTYGGAARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQLS
CCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
YAIGVADPTSIMVETFGTGKVANELLVSLVREFFDLRPYGLIKMLDLIQPIYRETAAYGH
EEEECCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCC
FGREQFPWEKVDRAEDLRVAAGLK
CCCCCCCHHHHCCHHHHHHHCCCC
>Mature Secondary Structure 
SSYLFTSESVSEGHPDKIADQISDAVLDEILKQDPKARVACETYVKTGMALVGGEITTS
CCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCHHHCCCCCCHH
AWVDIENLTRKVICDIGYEHSEMGFDGHSCAVLNAIGKQSADINQGVDRENPLDQGAGDQ
HEEEHHHHHHHHHHHCCCCHHHCCCCCCCHHHHHHHCCCCCCHHCCCCCCCCCCCCCCCC
GIMFGYATNETDVLMPAAITYAHRLMEKQAEVRKSGKLAWLRPDAKSQVTLKYEDNKIVG
CEEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCEEEEEECCCEEEE
VDAVVLSTQHSEEVSQKDLHEGVMEEIIKPVLPSEWLSKETKFFINPTGRFVIGGPMGDC
EEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCEEEECCCCCEEECCCCCCC
GLTGRKIIVDTYGGAARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQLS
CCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
YAIGVADPTSIMVETFGTGKVANELLVSLVREFFDLRPYGLIKMLDLIQPIYRETAAYGH
EEEECCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCC
FGREQFPWEKVDRAEDLRVAAGLK
CCCCCCCHHHHCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA