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The map label for this gene is ddl [H]

Identifier: 148828300

GI number: 148828300

Start: 1737710

End: 1738630

Strand: Direct

Name: ddl [H]

Synonym: CGSHiGG_09360

Alternate gene names: 148828300

Gene position: 1737710-1738630 (Clockwise)

Preceding gene: 148828299

Following gene: 148828301

Centisome position: 92.08

GC content: 39.41

Gene sequence:

>921_bases
ATGAACTTAAAACAAGAAAAAATCGCGGTGTTATTAGGCGGCACATCTGCTGAACGTGAAGTTTCTTTCAATTCAGGCAG
AGCGGTATTAGAAGCATTATTAAAGCAAGGTTATAATGCACATCCTATTGATCCTAAAGAATATAATGTTGCAAATCTTA
AAAAGGATGGCTTTAATCGAGCATTTAATATTTTGCACGGTCGTGGTGGAGAAGATGGCACAATGCAAGGTTTATTAGAG
CAAATTGGTTTGCCTTATACGGGGTGTGGCGTGATGGCTTCTGCATTAACGATGGACAAAATGCGTACAAAAATGTTGTG
GAAAGCTTTTGGTTTACCTGTTGCAGATATGAAAGTGGTTACCCGAGAAACTTTTTCTGAATTAGATCCTCAGGCTGTTG
TGGCAAAATTAGGCTTACCATTAATGGTTAAACCCTCTTTAGAAGGCTCTAGCGTTGGCTTAACGAAAGTAAAAGCGGTA
GAAGAGTTAAAAAGTGCGGTGGAATATGCGCTTAAATTTGATAATACCATTCTAATTGAAGAGTGGTTAGCTGGCGATGA
ATTAACTGTGCCTGTGCTTGACAATCAAGTATTACCCGCAATTCGTATTGTTCCAGAGGGCGAATTTTATGATTATGAAG
CGAAGTATATTTCTGATAATACGCAATATTTCTGCCCAGCAGGTTTAACGCCTGAACGCGAGCAAGAGTTAGCTATATTA
GTAAAACGCGCTTATGATGCAGTGGGATGTCGTGGTTGGAGTCGTATTGATGTAATGTGTGATGCAAAAGGTAATTTCCG
TTTGGTTGAGGTTAATACTAATCCTGGAATGACGAGCCATAGTTTATTCCCAAAATCTGCAGCGACAGTAGGTATTTCTT
TTGAGCAACTCGTCGTGAAAATTTTGGAGTTGAGCCTGTAA

Upstream 100 bases:

>100_bases
GTGGCTTGGCTGAATCTTGGAAGAATTAATGCCAGATGGATTGATTGAAAATCTTAGTATTAGAAAAGTATCATCAGTAA
GAAGAAAAACAGGATAGAAA

Downstream 100 bases:

>100_bases
TGAACATTCTGAAAAGAAAAACGCCACAGAATATTCGTTTTGGAGAACAAAAGCCTAAATATTATTTTCATATTCGGACT
TTTGCGGTATTGCTTGGTGT

Product: D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase [H]

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MNLKQEKIAVLLGGTSAEREVSFNSGRAVLEALLKQGYNAHPIDPKEYNVANLKKDGFNRAFNILHGRGGEDGTMQGLLE
QIGLPYTGCGVMASALTMDKMRTKMLWKAFGLPVADMKVVTRETFSELDPQAVVAKLGLPLMVKPSLEGSSVGLTKVKAV
EELKSAVEYALKFDNTILIEEWLAGDELTVPVLDNQVLPAIRIVPEGEFYDYEAKYISDNTQYFCPAGLTPEREQELAIL
VKRAYDAVGCRGWSRIDVMCDAKGNFRLVEVNTNPGMTSHSLFPKSAATVGISFEQLVVKILELSL

Sequences:

>Translated_306_residues
MNLKQEKIAVLLGGTSAEREVSFNSGRAVLEALLKQGYNAHPIDPKEYNVANLKKDGFNRAFNILHGRGGEDGTMQGLLE
QIGLPYTGCGVMASALTMDKMRTKMLWKAFGLPVADMKVVTRETFSELDPQAVVAKLGLPLMVKPSLEGSSVGLTKVKAV
EELKSAVEYALKFDNTILIEEWLAGDELTVPVLDNQVLPAIRIVPEGEFYDYEAKYISDNTQYFCPAGLTPEREQELAIL
VKRAYDAVGCRGWSRIDVMCDAKGNFRLVEVNTNPGMTSHSLFPKSAATVGISFEQLVVKILELSL
>Mature_306_residues
MNLKQEKIAVLLGGTSAEREVSFNSGRAVLEALLKQGYNAHPIDPKEYNVANLKKDGFNRAFNILHGRGGEDGTMQGLLE
QIGLPYTGCGVMASALTMDKMRTKMLWKAFGLPVADMKVVTRETFSELDPQAVVAKLGLPLMVKPSLEGSSVGLTKVKAV
EELKSAVEYALKFDNTILIEEWLAGDELTVPVLDNQVLPAIRIVPEGEFYDYEAKYISDNTQYFCPAGLTPEREQELAIL
VKRAYDAVGCRGWSRIDVMCDAKGNFRLVEVNTNPGMTSHSLFPKSAATVGISFEQLVVKILELSL

Specific function: Cell wall formation [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786280, Length=303, Percent_Identity=59.4059405940594, Blast_Score=369, Evalue=1e-103,
Organism=Escherichia coli, GI1786579, Length=351, Percent_Identity=31.3390313390313, Blast_Score=177, Evalue=1e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N [H]

EC number: =6.3.2.4 [H]

Molecular weight: Translated: 33607; Mature: 33607

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS50975 ATP_GRASP ; PS00843 DALA_DALA_LIGASE_1 ; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLKQEKIAVLLGGTSAEREVSFNSGRAVLEALLKQGYNAHPIDPKEYNVANLKKDGFNR
CCCCCCEEEEEECCCCCCCEECCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCHHH
AFNILHGRGGEDGTMQGLLEQIGLPYTGCGVMASALTMDKMRTKMLWKAFGLPVADMKVV
HHHHHCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
TRETFSELDPQAVVAKLGLPLMVKPSLEGSSVGLTKVKAVEELKSAVEYALKFDNTILIE
HHHHHHHCCHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEE
EWLAGDELTVPVLDNQVLPAIRIVPEGEFYDYEAKYISDNTQYFCPAGLTPEREQELAIL
HHCCCCCEEEEECCCCCCCEEEEEECCCCCCCEEEEECCCCCEEECCCCCCCHHHHHHHH
VKRAYDAVGCRGWSRIDVMCDAKGNFRLVEVNTNPGMTSHSLFPKSAATVGISFEQLVVK
HHHHHHHHCCCCCCEEEEEEECCCCEEEEEEECCCCCCCCCCCCCCHHEECCCHHHHHHH
ILELSL
HHHHCC
>Mature Secondary Structure
MNLKQEKIAVLLGGTSAEREVSFNSGRAVLEALLKQGYNAHPIDPKEYNVANLKKDGFNR
CCCCCCEEEEEECCCCCCCEECCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCHHH
AFNILHGRGGEDGTMQGLLEQIGLPYTGCGVMASALTMDKMRTKMLWKAFGLPVADMKVV
HHHHHCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
TRETFSELDPQAVVAKLGLPLMVKPSLEGSSVGLTKVKAVEELKSAVEYALKFDNTILIE
HHHHHHHCCHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEE
EWLAGDELTVPVLDNQVLPAIRIVPEGEFYDYEAKYISDNTQYFCPAGLTPEREQELAIL
HHCCCCCEEEEECCCCCCCEEEEEECCCCCCCEEEEECCCCCEEECCCCCCCHHHHHHHH
VKRAYDAVGCRGWSRIDVMCDAKGNFRLVEVNTNPGMTSHSLFPKSAATVGISFEQLVVK
HHHHHHHHCCCCCCEEEEEEECCCCEEEEEEECCCCCCCCCCCCCCHHEECCCHHHHHHH
ILELSL
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA