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The map label for this gene is thiP [H]

Identifier: 148828189

GI number: 148828189

Start: 1615223

End: 1616221

Strand: Direct

Name: thiP [H]

Synonym: CGSHiGG_08725

Alternate gene names: 148828189

Gene position: 1615223-1616221 (Clockwise)

Preceding gene: 148828188

Following gene: 148828198

Centisome position: 85.59

GC content: 41.54

Gene sequence:

>999_bases
ATGTTAGCGGAAAAATTACAAATTAACTCTATTACACCTCATCCAAGTGTAGAATATTGGTCTGTTTGCAAAGTTGAGGC
GTTATTTGAAACGCCATTTTTGGAATTAGTTTACCGAGCAGCGCAAATTCATCGTAAGCATTTTAACTCTCGCACGATTC
AGTTATCCACGTTAATGTCTATCAAAACAGGTGGATGCCCAGAAGATTGTGGTTATTGCCCTCAATCAGCCCGTTATCAT
ACTGGCGTACAAAATCAACAGTTATTAGATGTTAATGAAATTATTGCGAAAGCAAAAATTGCGAAAGCGCGTGGCGCAGG
GCGTTTCTGTATGGGCGCAGCTTGGCGAGGCCCTAAGCCAAAAGATATTGAGAAAGTCACAGAAATTATTAAAGCAGTGA
AATCGCTTGGTTTAGAAACTTGCGGTACCTTTGGTTTATTGCAAGACGGTATGGCAGAAGATTTAAAAGAAGCTGGATTA
GATTATTACAATCATAATCTTGATACCGCGCCAGAACATTATGCAGAAGTGATTGGTACTCGCCGTTTTGATGACCGCCT
TAGCACCTTAGGAAAAGTACGCAAAGCTGGGTTAAAAGTGTGTTGTGGCGGCATTGTGGGAATGAATGAAACTCGCAAAG
AACGGGCTGGATTAATCGCGAGCCTTGCAAATCTAGATCCGCAACCTGAGTCAGTGCCGATTAATCAACTCGTTAAGGTT
GAAGGTACACCCTTAGCCGATGCGGAAGAATTAGATTGGACGGAGTTTGTGCGAACGATTGCTGTTGCACGTATTACTAT
GCCAAAAAGTTATGTTCGCCTTTCTGCAGGTCGCAGTGGTATGACTGAAGAAATGCAAGCGATGTGTTTTATGGCGGGGG
CAAATTCGATTTTCTATGGCGATAAATTACTTGTTACAGATAATCCGGAAGAAGATGGCGATCAGTTATTAATGGCAAAA
TTAGATTTAGAGCCAGAAACTGCAGAAAATAAAAAGTAG

Upstream 100 bases:

>100_bases
TCATAACGTTGAATTGATTGTTTCTACCATATACACTAGTTCATCAATTAAGAAAGTATAAAAAGCGTGGTGTTTTTAAC
CGCACTTTCAAAGGAAAAAT

Downstream 100 bases:

>100_bases
TAATTTACTTTAGATAGTTGAGAAATTAAGTATAAATCTATTCCTTTCTTTATTTCATATCTCACAATAATTTTTAATAG
CCAATAAAATAGCTCCTTGA

Product: thiamin transporter membrane protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MLAEKLQINSITPHPSVEYWSVCKVEALFETPFLELVYRAAQIHRKHFNSRTIQLSTLMSIKTGGCPEDCGYCPQSARYH
TGVQNQQLLDVNEIIAKAKIAKARGAGRFCMGAAWRGPKPKDIEKVTEIIKAVKSLGLETCGTFGLLQDGMAEDLKEAGL
DYYNHNLDTAPEHYAEVIGTRRFDDRLSTLGKVRKAGLKVCCGGIVGMNETRKERAGLIASLANLDPQPESVPINQLVKV
EGTPLADAEELDWTEFVRTIAVARITMPKSYVRLSAGRSGMTEEMQAMCFMAGANSIFYGDKLLVTDNPEEDGDQLLMAK
LDLEPETAENKK

Sequences:

>Translated_332_residues
MLAEKLQINSITPHPSVEYWSVCKVEALFETPFLELVYRAAQIHRKHFNSRTIQLSTLMSIKTGGCPEDCGYCPQSARYH
TGVQNQQLLDVNEIIAKAKIAKARGAGRFCMGAAWRGPKPKDIEKVTEIIKAVKSLGLETCGTFGLLQDGMAEDLKEAGL
DYYNHNLDTAPEHYAEVIGTRRFDDRLSTLGKVRKAGLKVCCGGIVGMNETRKERAGLIASLANLDPQPESVPINQLVKV
EGTPLADAEELDWTEFVRTIAVARITMPKSYVRLSAGRSGMTEEMQAMCFMAGANSIFYGDKLLVTDNPEEDGDQLLMAK
LDLEPETAENKK
>Mature_332_residues
MLAEKLQINSITPHPSVEYWSVCKVEALFETPFLELVYRAAQIHRKHFNSRTIQLSTLMSIKTGGCPEDCGYCPQSARYH
TGVQNQQLLDVNEIIAKAKIAKARGAGRFCMGAAWRGPKPKDIEKVTEIIKAVKSLGLETCGTFGLLQDGMAEDLKEAGL
DYYNHNLDTAPEHYAEVIGTRRFDDRLSTLGKVRKAGLKVCCGGIVGMNETRKERAGLIASLANLDPQPESVPINQLVKV
EGTPLADAEELDWTEFVRTIAVARITMPKSYVRLSAGRSGMTEEMQAMCFMAGANSIFYGDKLLVTDNPEEDGDQLLMAK
LDLEPETAENKK

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism [H]

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786992, Length=307, Percent_Identity=59.2833876221498, Blast_Score=399, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6321725, Length=314, Percent_Identity=45.859872611465, Blast_Score=309, Evalue=4e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: =2.8.1.6 [H]

Molecular weight: Translated: 36660; Mature: 36660

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAEKLQINSITPHPSVEYWSVCKVEALFETPFLELVYRAAQIHRKHFNSRTIQLSTLMS
CCCCCEECCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCEEEEEHHEE
IKTGGCPEDCGYCPQSARYHTGVQNQQLLDVNEIIAKAKIAKARGAGRFCMGAAWRGPKP
ECCCCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCC
KDIEKVTEIIKAVKSLGLETCGTFGLLQDGMAEDLKEAGLDYYNHNLDTAPEHYAEVIGT
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCHHHHHHHHHC
RRFDDRLSTLGKVRKAGLKVCCGGIVGMNETRKERAGLIASLANLDPQPESVPINQLVKV
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHEEEE
EGTPLADAEELDWTEFVRTIAVARITMPKSYVRLSAGRSGMTEEMQAMCFMAGANSIFYG
CCCCCCCHHHCCHHHHHHHHHHHHHCCCHHHHEECCCCCCCHHHHHHHHHHHCCCEEEEC
DKLLVTDNPEEDGDQLLMAKLDLEPETAENKK
CEEEEECCCCCCCHHEEEEEECCCCCCCCCCC
>Mature Secondary Structure
MLAEKLQINSITPHPSVEYWSVCKVEALFETPFLELVYRAAQIHRKHFNSRTIQLSTLMS
CCCCCEECCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCEEEEEHHEE
IKTGGCPEDCGYCPQSARYHTGVQNQQLLDVNEIIAKAKIAKARGAGRFCMGAAWRGPKP
ECCCCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCC
KDIEKVTEIIKAVKSLGLETCGTFGLLQDGMAEDLKEAGLDYYNHNLDTAPEHYAEVIGT
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCHHHHHHHHHC
RRFDDRLSTLGKVRKAGLKVCCGGIVGMNETRKERAGLIASLANLDPQPESVPINQLVKV
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHEEEE
EGTPLADAEELDWTEFVRTIAVARITMPKSYVRLSAGRSGMTEEMQAMCFMAGANSIFYG
CCCCCCCHHHCCHHHHHHHHHHHHHCCCHHHHEECCCCCCCHHHHHHHHHHHCCCEEEEC
DKLLVTDNPEEDGDQLLMAKLDLEPETAENKK
CEEEEECCCCCCCHHEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA