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The map label for this gene is purD [H]

Identifier: 148828068

GI number: 148828068

Start: 1472733

End: 1474022

Strand: Direct

Name: purD [H]

Synonym: CGSHiGG_07990

Alternate gene names: 148828068

Gene position: 1472733-1474022 (Clockwise)

Preceding gene: 148828067

Following gene: 148828069

Centisome position: 78.04

GC content: 44.5

Gene sequence:

>1290_bases
ATGAACATCCTCATCATCGGAAACGGCGGTCGTGAACACGCTTTGGCTTGGAAAGCTGCACAATCTCCATTAGCAGATAA
AGTTTTTGTCGCACCGGGTAACGTGGGTACCGCATTGGAACACAAAGTAGAAAACGTGAATATTTCTGCGACAGATATTC
CAGCATTAGTGAAATTTGCTCAAGATAAACAAATTGGATTAACCATTGTTGGTCCTGAAGCCCCCTTAGTGATTGGGGTC
GTTGATGCATTTCGTGAAGCTGGATTGAAGATTTTTGGGCCAACTCAAGCAGCGGCACAATTAGAGGGTTCAAAAGCATT
TACTAAAGATTTCCTTGCTTGTCATAAAATTCCAACAGCGGAATATCAAAACTTTACCGAAGTCGAACCAGCATTGGCTT
ACTTGCGTGAGAAAGGCGCGCCAATTGTCGTTAAAGCGGATGGCTTGGCGGCGGGTAAAGGCGTAATCGTTGCGATGACA
TTGCAAGAAGCCGAAGATGCAGTACGCGATATGCTTTCTGGTAATGCCTTTGGCGAAGCGGGCAGCCGAGTAGTGATTGA
AGAATTTCTAGATGGCGAAGAAGCCAGTTTTATCGTCATGGTAGATGGTAAAAACGTAGAAGCTATGGCGACTAGCCAAG
ATCATAAACGCGTGGGCGAAAATGATACGGGGTTAAATACAGGCGGTATGGGTGCGTATTCGCCTGCACCAGTAATAACA
CCCGAAATTCATAGTCGCATTATGAAGGAAGTGATTTACCCAACCGTTAATGGCATGGCAGCGGAAGGCAGTGTTTACAC
GGGTTTCCTATATGCAGGATTAATGATTATGCCAAATGGGCAGCCGAAAGTGATCGAATTTAATTGTCGTTTTGGTGATC
CAGAAACGCAGCCAATAATGCTACGTCTCAAATCAGACCTTGTTGAACTCTGTCTTAAAGCATGTGACGGCAAATTAGAT
GAAGTAATATCACAATGGGATACACGAGCTTCTTTAGGTATCGTATTGGCTGCAGAGGGATACCCAAAAGATTATCGTAA
AGGCGATGAAATCAGCGGATTGCCTAAAAGTGCGGTCAAAAACGAGAAAGTTTTCTTAGCGGGTGTCGCAGAACAAGAAA
GCAAGCTAGTCACAAACGGCGGTCGTGTACTTTGTGTGACTGCGTTAGGCGAAAGTGTATTTGAAGCACAACAAAAAGCG
TTAAAACTGGCTGAGCAAATTCAATGGTCTGGGCGTTTTTATCGTCGAGATATTGGTTACAGGGCTGTGGAGCGAGAGTT
GCAAAAATAA

Upstream 100 bases:

>100_bases
TCGTTTAAATCTCCCCCTAACCCCTCTTTACAAAAGAGGGGAATTGCAGGTAAAAAATAGCCGCACTTTTTTTAAATTAG
GCATATACAAGGACTACAAC

Downstream 100 bases:

>100_bases
TCCATATCATTATGATGAATTAATTTCATTTAAGTGATGAAAATTTAGGAATGAAATTTTAATTAAATTTTTATCCATAA
ACAGTTAAACTATCTGCCAT

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MNILIIGNGGREHALAWKAAQSPLADKVFVAPGNVGTALEHKVENVNISATDIPALVKFAQDKQIGLTIVGPEAPLVIGV
VDAFREAGLKIFGPTQAAAQLEGSKAFTKDFLACHKIPTAEYQNFTEVEPALAYLREKGAPIVVKADGLAAGKGVIVAMT
LQEAEDAVRDMLSGNAFGEAGSRVVIEEFLDGEEASFIVMVDGKNVEAMATSQDHKRVGENDTGLNTGGMGAYSPAPVIT
PEIHSRIMKEVIYPTVNGMAAEGSVYTGFLYAGLMIMPNGQPKVIEFNCRFGDPETQPIMLRLKSDLVELCLKACDGKLD
EVISQWDTRASLGIVLAAEGYPKDYRKGDEISGLPKSAVKNEKVFLAGVAEQESKLVTNGGRVLCVTALGESVFEAQQKA
LKLAEQIQWSGRFYRRDIGYRAVERELQK

Sequences:

>Translated_429_residues
MNILIIGNGGREHALAWKAAQSPLADKVFVAPGNVGTALEHKVENVNISATDIPALVKFAQDKQIGLTIVGPEAPLVIGV
VDAFREAGLKIFGPTQAAAQLEGSKAFTKDFLACHKIPTAEYQNFTEVEPALAYLREKGAPIVVKADGLAAGKGVIVAMT
LQEAEDAVRDMLSGNAFGEAGSRVVIEEFLDGEEASFIVMVDGKNVEAMATSQDHKRVGENDTGLNTGGMGAYSPAPVIT
PEIHSRIMKEVIYPTVNGMAAEGSVYTGFLYAGLMIMPNGQPKVIEFNCRFGDPETQPIMLRLKSDLVELCLKACDGKLD
EVISQWDTRASLGIVLAAEGYPKDYRKGDEISGLPKSAVKNEKVFLAGVAEQESKLVTNGGRVLCVTALGESVFEAQQKA
LKLAEQIQWSGRFYRRDIGYRAVERELQK
>Mature_429_residues
MNILIIGNGGREHALAWKAAQSPLADKVFVAPGNVGTALEHKVENVNISATDIPALVKFAQDKQIGLTIVGPEAPLVIGV
VDAFREAGLKIFGPTQAAAQLEGSKAFTKDFLACHKIPTAEYQNFTEVEPALAYLREKGAPIVVKADGLAAGKGVIVAMT
LQEAEDAVRDMLSGNAFGEAGSRVVIEEFLDGEEASFIVMVDGKNVEAMATSQDHKRVGENDTGLNTGGMGAYSPAPVIT
PEIHSRIMKEVIYPTVNGMAAEGSVYTGFLYAGLMIMPNGQPKVIEFNCRFGDPETQPIMLRLKSDLVELCLKACDGKLD
EVISQWDTRASLGIVLAAEGYPKDYRKGDEISGLPKSAVKNEKVFLAGVAEQESKLVTNGGRVLCVTALGESVFEAQQKA
LKLAEQIQWSGRFYRRDIGYRAVERELQK

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI28416899, Length=424, Percent_Identity=50.4716981132076, Blast_Score=412, Evalue=1e-115,
Organism=Homo sapiens, GI4503915, Length=423, Percent_Identity=50.5910165484634, Blast_Score=412, Evalue=1e-115,
Organism=Homo sapiens, GI209869995, Length=423, Percent_Identity=50.5910165484634, Blast_Score=412, Evalue=1e-115,
Organism=Homo sapiens, GI209869993, Length=423, Percent_Identity=50.5910165484634, Blast_Score=412, Evalue=1e-115,
Organism=Escherichia coli, GI1790438, Length=427, Percent_Identity=74.9414519906323, Blast_Score=654, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17567511, Length=426, Percent_Identity=42.7230046948357, Blast_Score=319, Evalue=2e-87,
Organism=Saccharomyces cerevisiae, GI6321203, Length=444, Percent_Identity=41.4414414414414, Blast_Score=346, Evalue=4e-96,
Organism=Drosophila melanogaster, GI62471619, Length=431, Percent_Identity=45.9396751740139, Blast_Score=348, Evalue=4e-96,
Organism=Drosophila melanogaster, GI24582400, Length=431, Percent_Identity=46.1716937354988, Blast_Score=347, Evalue=6e-96,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 46144; Mature: 46144

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNILIIGNGGREHALAWKAAQSPLADKVFVAPGNVGTALEHKVENVNISATDIPALVKFA
CEEEEEECCCCCCCEEEHHHCCCCCCEEEECCCCCCHHHHHHHCCCEEECCCHHHHHHHH
QDKQIGLTIVGPEAPLVIGVVDAFREAGLKIFGPTQAAAQLEGSKAFTKDFLACHKIPTA
CCCCCCEEEECCCCCEEEEHHHHHHHCCCEEECCCHHHHHCCCCHHHHHHHHHHHCCCCH
EYQNFTEVEPALAYLREKGAPIVVKADGLAAGKGVIVAMTLQEAEDAVRDMLSGNAFGEA
HCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEHHHHHHHHHHHHCCCCCCCC
GSRVVIEEFLDGEEASFIVMVDGKNVEAMATSQDHKRVGENDTGLNTGGMGAYSPAPVIT
CCEEEHHHHCCCCCCEEEEEECCCCEEEEECCCHHHHCCCCCCCCCCCCCCCCCCCCCCC
PEIHSRIMKEVIYPTVNGMAAEGSVYTGFLYAGLMIMPNGQPKVIEFNCRFGDPETQPIM
HHHHHHHHHHHHCCCCCCEECCCCEEEEEEHCCEEEEECCCCEEEEEEEECCCCCCCCEE
LRLKSDLVELCLKACDGKLDEVISQWDTRASLGIVLAAEGYPKDYRKGDEISGLPKSAVK
EEHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEEEEECCCCHHHCCCCCCCCCCHHHHC
NEKVFLAGVAEQESKLVTNGGRVLCVTALGESVFEAQQKALKLAEQIQWSGRFYRRDIGY
CCEEEEEECCCCCCEEEECCCCEEEEEHHCHHHHHHHHHHHHHHHHHHHCCCHHHHHCCH
RAVERELQK
HHHHHHHCC
>Mature Secondary Structure
MNILIIGNGGREHALAWKAAQSPLADKVFVAPGNVGTALEHKVENVNISATDIPALVKFA
CEEEEEECCCCCCCEEEHHHCCCCCCEEEECCCCCCHHHHHHHCCCEEECCCHHHHHHHH
QDKQIGLTIVGPEAPLVIGVVDAFREAGLKIFGPTQAAAQLEGSKAFTKDFLACHKIPTA
CCCCCCEEEECCCCCEEEEHHHHHHHCCCEEECCCHHHHHCCCCHHHHHHHHHHHCCCCH
EYQNFTEVEPALAYLREKGAPIVVKADGLAAGKGVIVAMTLQEAEDAVRDMLSGNAFGEA
HCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEHHHHHHHHHHHHCCCCCCCC
GSRVVIEEFLDGEEASFIVMVDGKNVEAMATSQDHKRVGENDTGLNTGGMGAYSPAPVIT
CCEEEHHHHCCCCCCEEEEEECCCCEEEEECCCHHHHCCCCCCCCCCCCCCCCCCCCCCC
PEIHSRIMKEVIYPTVNGMAAEGSVYTGFLYAGLMIMPNGQPKVIEFNCRFGDPETQPIM
HHHHHHHHHHHHCCCCCCEECCCCEEEEEEHCCEEEEECCCCEEEEEEEECCCCCCCCEE
LRLKSDLVELCLKACDGKLDEVISQWDTRASLGIVLAAEGYPKDYRKGDEISGLPKSAVK
EEHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEEEEECCCCHHHCCCCCCCCCCHHHHC
NEKVFLAGVAEQESKLVTNGGRVLCVTALGESVFEAQQKALKLAEQIQWSGRFYRRDIGY
CCEEEEEECCCCCCEEEECCCCEEEEEHHCHHHHHHHHHHHHHHHHHHHCCCHHHHHCCH
RAVERELQK
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]