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The map label for this gene is purH [H]

Identifier: 148828067

GI number: 148828067

Start: 1470950

End: 1472548

Strand: Direct

Name: purH [H]

Synonym: CGSHiGG_07985

Alternate gene names: 148828067

Gene position: 1470950-1472548 (Clockwise)

Preceding gene: 148828066

Following gene: 148828068

Centisome position: 77.94

GC content: 42.53

Gene sequence:

>1599_bases
ATGGCAGATCGTCCAATTCGTCAGGCTTTACTGAGTGTGTCTGATAAAACGGGTATTGTAGAGTTTGCTCAAGGTTTAGT
TAAACGTGGTGTAAAACTACTTTCAACAGGTGGAACGGCAAAACTTTTAGCACAAAATGGTTTACCTGTAATGGAAGTGT
CTGATTACACAGGTTTCCCAGAAATGATGGACGGTCGAGTGAAAACCTTACATCCCAAAGTACATGGCGGGATTCTTGGT
CGTCGTGGTACAGATGATGCCATCATGCAGCAACATGGCATTGAAGGCATTGATATGGTCGTTGTGAATTTATATCCATT
TGCAGCCACCGTGGCAAAACCTGATTGCACTTTGGCTGATGCGGTAGAAAATATCGATATTGGAGGTCCAACAATGGTGC
GTTCTGCAGCGAAAAACCACAAAGATGTGGCGATCGTAGTTAATAACAATGATTTCAACGCAATTCTAGCCGAAATGGAT
CAACATCAAAACAGCCTAACTTTTGAAACTCGTTTTGACCTTGCGATTAAAGCATTTGAACATACCGCTCAATATGATTC
TATGATTGCCAACTATTTCGGTCAGCTAGTAAAACCTTATCATATTGCAGAGGAAGAAGAGGCGAATGCGAAGTGCGGTC
AATTCCCACGGACTTTAAACCTTAACTTCGTGCGTAAACAAGCTATGCGTTACGGCGAAAACGCCCATCAAAATGCGGCA
TTTTATGTTGATTTAAATGTGAAAGAAGCGAGCGTGGCTACAGCTAATCAATTGCAAGGTAAAGCCTTGTCTTACAATAA
TATTGCCGACACTGATGCAGCACTTGAATGCGTGAAAGAATTTGACGATCCAGCTTGCGTAATCGTTAAACATGCCAATC
CATGTGGTGTGGCGTTAGGTAAGGATATTTTAGACGCTTATAATCGCGCTTACCAAACCGATCCAACGTCTGCTTTTGGC
GGCATTATTGCTTTTAACCGTGAATTAGACGAAAAAACGGCGAATGAAATTGTGGAACGCCAATTCGTTGAAGTGATTAT
CGCACCGAAAGTTTCTGCTGAAGCGCAAGAAGTAATGAAGCGTAAGAAAAATGTGCGTTTGCTTGAATGTGGTGAATGGA
CTTCTCGTTCCGAACGTTTGGATTTCAAACGTGTAAACGGCGGTTTATTAGTACAAGATGCGGATTTAGGTATGGTTGGC
GTGGATGATTTAAAAGTCGTGAGTAAACGTCAGCCAACTGAACAAGAATTAAAAGACTTATTATTCTGCTGGAAAGTGGC
AAAATTTGTGAAATCCAATGCCATTGTTTACGCCAAAGACAATCAAACTATCGGCATTGGTGCAGGTCAAATGAGCCGTG
TATATTCTGCGAAGATTGCGGGTATTAAAGCACAGGATGAAGGCTTGACCGTGGCAGGTTGTGTGATGGCATCTGATGCG
TTCTTCCCATTCCGTGATGGCATTGATGCGGCAGCGAAAGTGGGGATTCAATGTGTAATCCATCCAGGTGGTTCAATGCG
CGATCAAGAAGTCATTGATGCGGCGGATGAACATAATATGGTGATGGTATTGACTGGAATGCGACATTTTAGACATTAA

Upstream 100 bases:

>100_bases
CACTTTAAGAAATTATATTTAATCTTTTCTATATTTAAGCAAACGTTTGCGTTATAATTCTCATCGCTTATTTTTAACTA
TTTAATGAAAGGAAAATACA

Downstream 100 bases:

>100_bases
TTTATCTAACTATCCCCCTCTTTAGCAAAGAGGGGAAGGGGAGATTTTGGCAGAAGTGATTTCAAGCTCATACTAAATTC
AATATCGTTTAAATCTCCCC

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 532; Mature: 531

Protein sequence:

>532_residues
MADRPIRQALLSVSDKTGIVEFAQGLVKRGVKLLSTGGTAKLLAQNGLPVMEVSDYTGFPEMMDGRVKTLHPKVHGGILG
RRGTDDAIMQQHGIEGIDMVVVNLYPFAATVAKPDCTLADAVENIDIGGPTMVRSAAKNHKDVAIVVNNNDFNAILAEMD
QHQNSLTFETRFDLAIKAFEHTAQYDSMIANYFGQLVKPYHIAEEEEANAKCGQFPRTLNLNFVRKQAMRYGENAHQNAA
FYVDLNVKEASVATANQLQGKALSYNNIADTDAALECVKEFDDPACVIVKHANPCGVALGKDILDAYNRAYQTDPTSAFG
GIIAFNRELDEKTANEIVERQFVEVIIAPKVSAEAQEVMKRKKNVRLLECGEWTSRSERLDFKRVNGGLLVQDADLGMVG
VDDLKVVSKRQPTEQELKDLLFCWKVAKFVKSNAIVYAKDNQTIGIGAGQMSRVYSAKIAGIKAQDEGLTVAGCVMASDA
FFPFRDGIDAAAKVGIQCVIHPGGSMRDQEVIDAADEHNMVMVLTGMRHFRH

Sequences:

>Translated_532_residues
MADRPIRQALLSVSDKTGIVEFAQGLVKRGVKLLSTGGTAKLLAQNGLPVMEVSDYTGFPEMMDGRVKTLHPKVHGGILG
RRGTDDAIMQQHGIEGIDMVVVNLYPFAATVAKPDCTLADAVENIDIGGPTMVRSAAKNHKDVAIVVNNNDFNAILAEMD
QHQNSLTFETRFDLAIKAFEHTAQYDSMIANYFGQLVKPYHIAEEEEANAKCGQFPRTLNLNFVRKQAMRYGENAHQNAA
FYVDLNVKEASVATANQLQGKALSYNNIADTDAALECVKEFDDPACVIVKHANPCGVALGKDILDAYNRAYQTDPTSAFG
GIIAFNRELDEKTANEIVERQFVEVIIAPKVSAEAQEVMKRKKNVRLLECGEWTSRSERLDFKRVNGGLLVQDADLGMVG
VDDLKVVSKRQPTEQELKDLLFCWKVAKFVKSNAIVYAKDNQTIGIGAGQMSRVYSAKIAGIKAQDEGLTVAGCVMASDA
FFPFRDGIDAAAKVGIQCVIHPGGSMRDQEVIDAADEHNMVMVLTGMRHFRH
>Mature_531_residues
ADRPIRQALLSVSDKTGIVEFAQGLVKRGVKLLSTGGTAKLLAQNGLPVMEVSDYTGFPEMMDGRVKTLHPKVHGGILGR
RGTDDAIMQQHGIEGIDMVVVNLYPFAATVAKPDCTLADAVENIDIGGPTMVRSAAKNHKDVAIVVNNNDFNAILAEMDQ
HQNSLTFETRFDLAIKAFEHTAQYDSMIANYFGQLVKPYHIAEEEEANAKCGQFPRTLNLNFVRKQAMRYGENAHQNAAF
YVDLNVKEASVATANQLQGKALSYNNIADTDAALECVKEFDDPACVIVKHANPCGVALGKDILDAYNRAYQTDPTSAFGG
IIAFNRELDEKTANEIVERQFVEVIIAPKVSAEAQEVMKRKKNVRLLECGEWTSRSERLDFKRVNGGLLVQDADLGMVGV
DDLKVVSKRQPTEQELKDLLFCWKVAKFVKSNAIVYAKDNQTIGIGAGQMSRVYSAKIAGIKAQDEGLTVAGCVMASDAF
FPFRDGIDAAAKVGIQCVIHPGGSMRDQEVIDAADEHNMVMVLTGMRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=491, Percent_Identity=38.2892057026477, Blast_Score=269, Evalue=5e-72,
Organism=Escherichia coli, GI1790439, Length=529, Percent_Identity=76.937618147448, Blast_Score=845, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71985564, Length=623, Percent_Identity=32.5842696629214, Blast_Score=258, Evalue=7e-69,
Organism=Caenorhabditis elegans, GI71985574, Length=351, Percent_Identity=27.0655270655271, Blast_Score=89, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI71985556, Length=119, Percent_Identity=36.1344537815126, Blast_Score=78, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323768, Length=492, Percent_Identity=34.5528455284553, Blast_Score=258, Evalue=2e-69,
Organism=Saccharomyces cerevisiae, GI6323056, Length=496, Percent_Identity=35.2822580645161, Blast_Score=254, Evalue=2e-68,
Organism=Drosophila melanogaster, GI24649832, Length=501, Percent_Identity=38.5229540918164, Blast_Score=282, Evalue=3e-76,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 58287; Mature: 58156

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADRPIRQALLSVSDKTGIVEFAQGLVKRGVKLLSTGGTAKLLAQNGLPVMEVSDYTGFP
CCCCHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEECCCCEEEEECCCCCEEEECCCCCCH
EMMDGRVKTLHPKVHGGILGRRGTDDAIMQQHGIEGIDMVVVNLYPFAATVAKPDCTLAD
HHHCCCEEEECCHHHCCCCCCCCCCHHHHHHCCCCCEEEEEEEEEHHHHHCCCCCCHHHH
AVENIDIGGPTMVRSAAKNHKDVAIVVNNNDFNAILAEMDQHQNSLTFETRFDLAIKAFE
HHHHCCCCCHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHCCCCEEEEEEHHHHHHHHH
HTAQYDSMIANYFGQLVKPYHIAEEEEANAKCGQFPRTLNLNFVRKQAMRYGENAHQNAA
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEE
FYVDLNVKEASVATANQLQGKALSYNNIADTDAALECVKEFDDPACVIVKHANPCGVALG
EEEEECCCHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCHHHH
KDILDAYNRAYQTDPTSAFGGIIAFNRELDEKTANEIVERQFVEVIIAPKVSAEAQEVMK
HHHHHHHHHHCCCCCCHHHCCEEEECCHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHH
RKKNVRLLECGEWTSRSERLDFKRVNGGLLVQDADLGMVGVDDLKVVSKRQPTEQELKDL
HHCCCEEEECCCCCCCCCCCCCEECCCCEEEEECCCCEECCCHHHHHHCCCCCHHHHHHH
LFCWKVAKFVKSNAIVYAKDNQTIGIGAGQMSRVYSAKIAGIKAQDEGLTVAGCVMASDA
HHHHHHHHHHHCCCEEEEECCCEEEECCCHHHHHHHHHHCCEEECCCCCEEEEEEEECCC
FFPFRDGIDAAAKVGIQCVIHPGGSMRDQEVIDAADEHNMVMVLTGMRHFRH
CCCCCCCCCHHHCCCEEEEECCCCCCCCHHHHCCCCCCCEEEEEECHHHHCC
>Mature Secondary Structure 
ADRPIRQALLSVSDKTGIVEFAQGLVKRGVKLLSTGGTAKLLAQNGLPVMEVSDYTGFP
CCCHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEECCCCEEEEECCCCCEEEECCCCCCH
EMMDGRVKTLHPKVHGGILGRRGTDDAIMQQHGIEGIDMVVVNLYPFAATVAKPDCTLAD
HHHCCCEEEECCHHHCCCCCCCCCCHHHHHHCCCCCEEEEEEEEEHHHHHCCCCCCHHHH
AVENIDIGGPTMVRSAAKNHKDVAIVVNNNDFNAILAEMDQHQNSLTFETRFDLAIKAFE
HHHHCCCCCHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHCCCCEEEEEEHHHHHHHHH
HTAQYDSMIANYFGQLVKPYHIAEEEEANAKCGQFPRTLNLNFVRKQAMRYGENAHQNAA
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEE
FYVDLNVKEASVATANQLQGKALSYNNIADTDAALECVKEFDDPACVIVKHANPCGVALG
EEEEECCCHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCHHHH
KDILDAYNRAYQTDPTSAFGGIIAFNRELDEKTANEIVERQFVEVIIAPKVSAEAQEVMK
HHHHHHHHHHCCCCCCHHHCCEEEECCHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHH
RKKNVRLLECGEWTSRSERLDFKRVNGGLLVQDADLGMVGVDDLKVVSKRQPTEQELKDL
HHCCCEEEECCCCCCCCCCCCCEECCCCEEEEECCCCEECCCHHHHHHCCCCCHHHHHHH
LFCWKVAKFVKSNAIVYAKDNQTIGIGAGQMSRVYSAKIAGIKAQDEGLTVAGCVMASDA
HHHHHHHHHHHCCCEEEEECCCEEEECCCHHHHHHHHHHCCEEECCCCCEEEEEEEECCC
FFPFRDGIDAAAKVGIQCVIHPGGSMRDQEVIDAADEHNMVMVLTGMRHFRH
CCCCCCCCCHHHCCCEEEEECCCCCCCCHHHHCCCCCCCEEEEEECHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]