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The map label for this gene is arcB [H]

Identifier: 148827789

GI number: 148827789

Start: 1144263

End: 1145267

Strand: Direct

Name: arcB [H]

Synonym: CGSHiGG_06290

Alternate gene names: 148827789

Gene position: 1144263-1145267 (Clockwise)

Preceding gene: 148827786

Following gene: 148827790

Centisome position: 60.63

GC content: 39.0

Gene sequence:

>1005_bases
ATGGCTTTCAATATGAAAAATAGACATTTACTTAGTCTTGTTCATCACACAGAACGTGAAATTAAATATCTCTTAGATCT
CTCCCGAGATTTGAAACGAGCCAAATATGCCGGCACAGAACAACAAAAATTAAAAGGCAAAAATATCGCGCTCATCTTTG
AAAAAACCTCCACCCGCACACGCTGTGCATTTGAAGTCGCTGCTTATGATCAAGGCGCACAAGTCACCTACATCGACCCT
AATTCCTCTCAAATTGGCCACAAAGAAAGTATGAAAGATACCGCCCGCGTATTAGGCAGAATGTATGATGGCATTGAATA
TCGTGGCTTTAAACAAAGTATCGTTCAAGAACTGGCTGATTATGCTGGAGTGCCAGTATTCAATGGTTTAACCGATGAAT
TCCACCCAACACAAATGCTTGCCGACGTACTTACCATGATTGAACATTGCGACAAACCATTAAGTGAAATTAGTTATGTA
TATATTGGGGATGCTCGCAATAACATGGGTAATTCACTTTTATTAATTGGTGCAAAATTAGGTATGGATGTTCGTATTTG
TGCACCTAAAGCATTATTACCAGAAGCAAGTCTTGTGGAAATGTGCGAAAAATTTGCGAAAGAAAGTGGAGCTCGTATTA
CTGTAACTGAAGATATCGACAAAGCGGTGAAAGGCGTAGATTTTGTGCATACTGATGTGTGGGTTTCAATGGGTGAGCCA
TTAGAAACTTGGGGCGAACGCATTAAATTATTACTTCCTTATCAAGTCACACCTGAATTAATGAAACGCACTGGTAATCC
AAAAGTGAAATTTATGCACTGCTTGCCAGCCTTCCATAACAGTGAAACAAAAGTGGGTCGTCAAATTGCTGAAAAATATC
CTGAATTAGCTAATGGTATTGAAGTAACTGAAGAGGTATTTGAATCGCCAATGAACATTGCATTTGAACAAGCAGAAAAC
CGTATGCACACAATTAAAGCGGTAATGGTAGCAAGTTTGGCATAA

Upstream 100 bases:

>100_bases
ATTTTACCTCTCTTATAATGCAACTGTTCGAACAACAAGCAGTATCTCGAATGATGTATGTTTATTCCCCAATGTATAGT
CAAAACACAAGGAGATTATT

Downstream 100 bases:

>100_bases
GGAGAACGTATGAGAATAGTGATTGCATTAGGCGGTAATGCGTTATTACGACGCGGAGAACCAATGACAGCCGAAAATCA
ACGACAAAATGTTCGCATTG

Product: ornithine carbamoyltransferase

Products: NA

Alternate protein names: OTCase [H]

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MAFNMKNRHLLSLVHHTEREIKYLLDLSRDLKRAKYAGTEQQKLKGKNIALIFEKTSTRTRCAFEVAAYDQGAQVTYIDP
NSSQIGHKESMKDTARVLGRMYDGIEYRGFKQSIVQELADYAGVPVFNGLTDEFHPTQMLADVLTMIEHCDKPLSEISYV
YIGDARNNMGNSLLLIGAKLGMDVRICAPKALLPEASLVEMCEKFAKESGARITVTEDIDKAVKGVDFVHTDVWVSMGEP
LETWGERIKLLLPYQVTPELMKRTGNPKVKFMHCLPAFHNSETKVGRQIAEKYPELANGIEVTEEVFESPMNIAFEQAEN
RMHTIKAVMVASLA

Sequences:

>Translated_334_residues
MAFNMKNRHLLSLVHHTEREIKYLLDLSRDLKRAKYAGTEQQKLKGKNIALIFEKTSTRTRCAFEVAAYDQGAQVTYIDP
NSSQIGHKESMKDTARVLGRMYDGIEYRGFKQSIVQELADYAGVPVFNGLTDEFHPTQMLADVLTMIEHCDKPLSEISYV
YIGDARNNMGNSLLLIGAKLGMDVRICAPKALLPEASLVEMCEKFAKESGARITVTEDIDKAVKGVDFVHTDVWVSMGEP
LETWGERIKLLLPYQVTPELMKRTGNPKVKFMHCLPAFHNSETKVGRQIAEKYPELANGIEVTEEVFESPMNIAFEQAEN
RMHTIKAVMVASLA
>Mature_333_residues
AFNMKNRHLLSLVHHTEREIKYLLDLSRDLKRAKYAGTEQQKLKGKNIALIFEKTSTRTRCAFEVAAYDQGAQVTYIDPN
SSQIGHKESMKDTARVLGRMYDGIEYRGFKQSIVQELADYAGVPVFNGLTDEFHPTQMLADVLTMIEHCDKPLSEISYVY
IGDARNNMGNSLLLIGAKLGMDVRICAPKALLPEASLVEMCEKFAKESGARITVTEDIDKAVKGVDFVHTDVWVSMGEPL
ETWGERIKLLLPYQVTPELMKRTGNPKVKFMHCLPAFHNSETKVGRQIAEKYPELANGIEVTEEVFESPMNIAFEQAENR
MHTIKAVMVASLA

Specific function: Arginine biosynthesis; sixth step. [C]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI38788445, Length=330, Percent_Identity=39.3939393939394, Blast_Score=213, Evalue=3e-55,
Organism=Homo sapiens, GI18105007, Length=338, Percent_Identity=23.0769230769231, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1790703, Length=329, Percent_Identity=62.0060790273556, Blast_Score=431, Evalue=1e-122,
Organism=Escherichia coli, GI1786469, Length=332, Percent_Identity=60.5421686746988, Blast_Score=422, Evalue=1e-119,
Organism=Escherichia coli, GI48994908, Length=383, Percent_Identity=23.2375979112272, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI2367364, Length=320, Percent_Identity=26.5625, Blast_Score=69, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6322373, Length=330, Percent_Identity=32.4242424242424, Blast_Score=160, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6322331, Length=195, Percent_Identity=27.1794871794872, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24642586, Length=333, Percent_Identity=22.5225225225225, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2066 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002292 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.3 [H]

Molecular weight: Translated: 37621; Mature: 37490

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFNMKNRHLLSLVHHTEREIKYLLDLSRDLKRAKYAGTEQQKLKGKNIALIFEKTSTRT
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCEEEEEECCCCCC
RCAFEVAAYDQGAQVTYIDPNSSQIGHKESMKDTARVLGRMYDGIEYRGFKQSIVQELAD
CEEEEEEECCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
YAGVPVFNGLTDEFHPTQMLADVLTMIEHCDKPLSEISYVYIGDARNNMGNSLLLIGAKL
HCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHCCEEEEECCCCCCCCEEEEEEECC
GMDVRICAPKALLPEASLVEMCEKFAKESGARITVTEDIDKAVKGVDFVHTDVWVSMGEP
CCEEEEECCHHHCCCHHHHHHHHHHHHCCCCEEEEEHHHHHHHHCCHHEEHHHHHCCCCC
LETWGERIKLLLPYQVTPELMKRTGNPKVKFMHCLPAFHNSETKVGRQIAEKYPELANGI
HHHHHHHEEEEECCCCCHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCHH
EVTEEVFESPMNIAFEQAENRMHTIKAVMVASLA
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AFNMKNRHLLSLVHHTEREIKYLLDLSRDLKRAKYAGTEQQKLKGKNIALIFEKTSTRT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCEEEEEECCCCCC
RCAFEVAAYDQGAQVTYIDPNSSQIGHKESMKDTARVLGRMYDGIEYRGFKQSIVQELAD
CEEEEEEECCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
YAGVPVFNGLTDEFHPTQMLADVLTMIEHCDKPLSEISYVYIGDARNNMGNSLLLIGAKL
HCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHCCEEEEECCCCCCCCEEEEEEECC
GMDVRICAPKALLPEASLVEMCEKFAKESGARITVTEDIDKAVKGVDFVHTDVWVSMGEP
CCEEEEECCHHHCCCHHHHHHHHHHHHCCCCEEEEEHHHHHHHHCCHHEEHHHHHCCCCC
LETWGERIKLLLPYQVTPELMKRTGNPKVKFMHCLPAFHNSETKVGRQIAEKYPELANGI
HHHHHHHEEEEECCCCCHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCHH
EVTEEVFESPMNIAFEQAENRMHTIKAVMVASLA
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]