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The map label for this gene is mscL

Identifier: 148827775

GI number: 148827775

Start: 1127538

End: 1127924

Strand: Reverse

Name: mscL

Synonym: CGSHiGG_06210

Alternate gene names: 148827775

Gene position: 1127924-1127538 (Counterclockwise)

Preceding gene: 148827776

Following gene: 148827774

Centisome position: 59.77

GC content: 33.07

Gene sequence:

>387_bases
ATGAATTTTATTAAAGAATTTCGCGAATTTGCAATGCGTGGCAATGTCGTAGATATGGCTATCGGTGTGATTATTGGCAG
CGCATTTGGCAAGATCGTAAGTTCACTTGTTAGTGATATTTTTACGCCCGTGTTGGGGATTTTAACTGGTGGTATTGATT
TCAAAGATATGAAATTTGTATTAGCACAAGCACAAGGCGATGTTCCTGCTGTTACTTTAAATTATGGTTTGTTTATTCAA
AATGTGATTGATTTTATTATTATTGCTTTTGCAATCTTTATGATGATCAAAGTAATCAATAAGGTAAGAAAACCAGAGGA
GAAAAAAACTGCACCAAAGGCAGAAACATTGCTTACTGAAATCCGTGATTTATTAAAGAATAAATAA

Upstream 100 bases:

>100_bases
ATTCCAACCAAGCGCATTCTTTATTTAGTTTTTTTACTTTTCAGAGTGATTTATTATAATAGTCGCTCTTTTTATGTAAT
TTTTGAAGGGGAACATTATT

Downstream 100 bases:

>100_bases
TATTTAAAGAATGAAAGTTAGCAGAAATGCTGACTTTTTTTTATTACTATTTTTTAAGCTATAATCAGCCCTCAATTCTA
GGGAAATAAAATGGAAAAAT

Product: large-conductance mechanosensitive channel

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 128; Mature: 128

Protein sequence:

>128_residues
MNFIKEFREFAMRGNVVDMAIGVIIGSAFGKIVSSLVSDIFTPVLGILTGGIDFKDMKFVLAQAQGDVPAVTLNYGLFIQ
NVIDFIIIAFAIFMMIKVINKVRKPEEKKTAPKAETLLTEIRDLLKNK

Sequences:

>Translated_128_residues
MNFIKEFREFAMRGNVVDMAIGVIIGSAFGKIVSSLVSDIFTPVLGILTGGIDFKDMKFVLAQAQGDVPAVTLNYGLFIQ
NVIDFIIIAFAIFMMIKVINKVRKPEEKKTAPKAETLLTEIRDLLKNK
>Mature_128_residues
MNFIKEFREFAMRGNVVDMAIGVIIGSAFGKIVSSLVSDIFTPVLGILTGGIDFKDMKFVLAQAQGDVPAVTLNYGLFIQ
NVIDFIIIAFAIFMMIKVINKVRKPEEKKTAPKAETLLTEIRDLLKNK

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family

Homologues:

Organism=Escherichia coli, GI1789686, Length=132, Percent_Identity=68.9393939393939, Blast_Score=175, Evalue=7e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MSCL_HAEI8 (Q4QMW0)

Other databases:

- EMBL:   CP000057
- RefSeq:   YP_248297.1
- ProteinModelPortal:   Q4QMW0
- SMR:   Q4QMW0
- STRING:   Q4QMW0
- GeneID:   3430029
- GenomeReviews:   CP000057_GR
- KEGG:   hit:NTHI0721
- eggNOG:   COG1970
- HOGENOM:   HBG727604
- OMA:   GANVLAW
- PhylomeDB:   Q4QMW0
- ProtClustDB:   PRK00567
- BioCyc:   HINF281310:NTHI0721-MONOMER
- HAMAP:   MF_00115
- InterPro:   IPR019823
- InterPro:   IPR001185
- PRINTS:   PR01264
- TIGRFAMs:   TIGR00220

Pfam domain/function: PF01741 MscL; SSF81330 MS_channel

EC number: NA

Molecular weight: Translated: 14212; Mature: 14212

Theoretical pI: Translated: 9.99; Mature: 9.99

Prosite motif: PS01327 MSCL

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x12d3da50)-; HASH(0x12c8a954)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFIKEFREFAMRGNVVDMAIGVIIGSAFGKIVSSLVSDIFTPVLGILTGGIDFKDMKFV
CCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
LAQAQGDVPAVTLNYGLFIQNVIDFIIIAFAIFMMIKVINKVRKPEEKKTAPKAETLLTE
HHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHH
IRDLLKNK
HHHHHCCH
>Mature Secondary Structure
MNFIKEFREFAMRGNVVDMAIGVIIGSAFGKIVSSLVSDIFTPVLGILTGGIDFKDMKFV
CCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
LAQAQGDVPAVTLNYGLFIQNVIDFIIIAFAIFMMIKVINKVRKPEEKKTAPKAETLLTE
HHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHH
IRDLLKNK
HHHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA