Click here to switch to the map view.

The map label for this gene is rpoE

Identifier: 148827773

GI number: 148827773

Start: 1126513

End: 1127082

Strand: Reverse

Name: rpoE

Synonym: CGSHiGG_06200

Alternate gene names: 148827773

Gene position: 1127082-1126513 (Counterclockwise)

Preceding gene: 148827774

Following gene: 148827772

Centisome position: 59.72

GC content: 38.6

Gene sequence:

>570_bases
ATGGCTGAACAGCTAACAGATCAGGCTTTGGTAGAAAGAGTACAGCAAGGGGATAAAAAAGCCTTCAATTTGTTGGTTTC
GCGTTATCAAAATAAAGTAGCAGGGCTTTTGACCCGATATGTATCGCGTAACGATATTCCTGATGTGGTGCAAGAATCCT
TTATAAAGGCTTATCGCTCAATTGAATCTTTTCGTGGCGAAAGTGCTTTCTATACATGGCTTTATCGAATTGCCGTTAAT
ACGGCAAAAAATTATTTAACGGCTCAAGGTCGCCGTCCACCAAATGAAGATATTTTGGCAGAAGATGCTGAAAATTATGA
TGTGGGAACACACCTTAGGGATGTGGATACACCCGAAAATGAGATGTTGTCTAGTGAGTTAGAAAGAATTGTTTTTGATA
CTATTCATAACTTACCAGAAGATTTAAAAACTGCGATTACGTTACGAGAACTAGAAGGTTTAAGTTACGAAGATATTGCA
GAAATTATGGATTGTCCTGTTGGTACGGTTCGTTCCCGAATCTTCCGAGCAAGGGAAATGATCGAAAATAAAATTCAACC
TCTAATGTAG

Upstream 100 bases:

>100_bases
GATTAGCGAATGAACTTTTTTAACACGCTTTTGTCTGAACAAGCAACAAGAGCTTGCTGTCTTGATATTAAGGCGTGTTA
GATATTGGCATGGGAGAAAT

Downstream 100 bases:

>100_bases
AGCGAATAACAATCGGAGTTTACTATGCAAAAAGAGCAGCTTTCAGCCTATATGGATGGAGAACAAGTCGAAACTGACTT
GATAGATGCTTTATTAAGAG

Product: RNA polymerase sigma factor RpoE

Products: NA

Alternate protein names: Sigma-24

Number of amino acids: Translated: 189; Mature: 188

Protein sequence:

>189_residues
MAEQLTDQALVERVQQGDKKAFNLLVSRYQNKVAGLLTRYVSRNDIPDVVQESFIKAYRSIESFRGESAFYTWLYRIAVN
TAKNYLTAQGRRPPNEDILAEDAENYDVGTHLRDVDTPENEMLSSELERIVFDTIHNLPEDLKTAITLRELEGLSYEDIA
EIMDCPVGTVRSRIFRAREMIENKIQPLM

Sequences:

>Translated_189_residues
MAEQLTDQALVERVQQGDKKAFNLLVSRYQNKVAGLLTRYVSRNDIPDVVQESFIKAYRSIESFRGESAFYTWLYRIAVN
TAKNYLTAQGRRPPNEDILAEDAENYDVGTHLRDVDTPENEMLSSELERIVFDTIHNLPEDLKTAITLRELEGLSYEDIA
EIMDCPVGTVRSRIFRAREMIENKIQPLM
>Mature_188_residues
AEQLTDQALVERVQQGDKKAFNLLVSRYQNKVAGLLTRYVSRNDIPDVVQESFIKAYRSIESFRGESAFYTWLYRIAVNT
AKNYLTAQGRRPPNEDILAEDAENYDVGTHLRDVDTPENEMLSSELERIVFDTIHNLPEDLKTAITLRELEGLSYEDIAE
IMDCPVGTVRSRIFRAREMIENKIQPLM

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is involved in heat shock and oxidative stress response; it is believed to control protein processing

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily

Homologues:

Organism=Escherichia coli, GI1788926, Length=189, Percent_Identity=73.015873015873, Blast_Score=296, Evalue=8e-82,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): RPOE_HAEIN (P44790)

Other databases:

- EMBL:   L42023
- PIR:   F64082
- RefSeq:   NP_438788.1
- ProteinModelPortal:   P44790
- SMR:   P44790
- GeneID:   950801
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0628
- NMPDR:   fig|71421.1.peg.598
- TIGR:   HI_0628
- HOGENOM:   HBG753452
- OMA:   ISARVKP
- ProtClustDB:   PRK09652
- BioCyc:   HINF71421:HI_0628-MONOMER
- InterPro:   IPR014284
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR014286
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- TIGRFAMs:   TIGR02939
- TIGRFAMs:   TIGR02937

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2; SSF88946 Sigma_r2; SSF88659 Sigma_r3_r4

EC number: NA

Molecular weight: Translated: 21792; Mature: 21661

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS01063 SIGMA70_ECF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEQLTDQALVERVQQGDKKAFNLLVSRYQNKVAGLLTRYVSRNDIPDVVQESFIKAYRS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
IESFRGESAFYTWLYRIAVNTAKNYLTAQGRRPPNEDILAEDAENYDVGTHLRDVDTPEN
HHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCHH
EMLSSELERIVFDTIHNLPEDLKTAITLRELEGLSYEDIAEIMDCPVGTVRSRIFRAREM
HHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHH
IENKIQPLM
HHHHHCCCC
>Mature Secondary Structure 
AEQLTDQALVERVQQGDKKAFNLLVSRYQNKVAGLLTRYVSRNDIPDVVQESFIKAYRS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
IESFRGESAFYTWLYRIAVNTAKNYLTAQGRRPPNEDILAEDAENYDVGTHLRDVDTPEN
HHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCHH
EMLSSELERIVFDTIHNLPEDLKTAITLRELEGLSYEDIAEIMDCPVGTVRSRIFRAREM
HHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHH
IENKIQPLM
HHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800