Click here to switch to the map view.

The map label for this gene is gidB

Identifier: 148827683

GI number: 148827683

Start: 1033058

End: 1033669

Strand: Reverse

Name: gidB

Synonym: CGSHiGG_05690

Alternate gene names: 148827683

Gene position: 1033669-1033058 (Counterclockwise)

Preceding gene: 148827684

Following gene: 148827682

Centisome position: 54.77

GC content: 32.35

Gene sequence:

>612_bases
ATGAAAGCCAAATTAGTCAGTTTACTTGCGCAAGCAAATATAAAAATAAGTGATCAACAAATTCAGCAGTTGATTAATCT
GGTCAATTTACTCAATAAATGGAATAAAGCTTATAATTTGACGTCAGTTCGTGATCCACAAGAAATGTTAGTTAAACATA
TTTTAGATAGTCTTGTTGTAAGTCCTTATTTACAAGGTGATCGTTTTATTGATGTCGGTACAGGGCCTGGTTTGCCTGGT
TTACCTTTGGCAATTATTAATCCTTCCAAACAATTTGTCCTCCTTGATAGTTTAGGCAAGCGTATTAGTTTTATTCGAAA
TGCTATACGTGAGCTTAGACTAACTAATGTAACCCCCGTTTTAAGTCGAGTAGAAGAATATCAACCTGAAGATAAATTTG
ATGGCGTATTAAGCCGTGCTTTTGCTTCACTAAAAGATATGACAGATTGGTGCCATCATTTACCAAAAGAAAATGGATAT
TTTTATGCATTAAAAGGGATTTATCAGGAAGATGAAATTAATGAATTGAATAAAAAATATACTATTCAAAAAGTGATTGA
ATTATCTGTTCCTGAACTTATAGGCGAACGACATTTAATCGTTTTACGTTAA

Upstream 100 bases:

>100_bases
GGACATTGATTCGGTAAATAATTCTCAAATTACTAAAACATTAAGGAAATTTACCGCACTTTTAAGTAAAATAGCGTAAT
TTTTCAAAGAGATAATAAAT

Downstream 100 bases:

>100_bases
AAAGTAACAAATAGATAAATTATTTTCTAAAAGTGTGATTTATATAACACTTTTTATGTGTTTTTAAGTTGAATCTAAAG
CCAGCAAAGGTATAATCGGC

Product: 16S rRNA methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase

Number of amino acids: Translated: 203; Mature: 203

Protein sequence:

>203_residues
MKAKLVSLLAQANIKISDQQIQQLINLVNLLNKWNKAYNLTSVRDPQEMLVKHILDSLVVSPYLQGDRFIDVGTGPGLPG
LPLAIINPSKQFVLLDSLGKRISFIRNAIRELRLTNVTPVLSRVEEYQPEDKFDGVLSRAFASLKDMTDWCHHLPKENGY
FYALKGIYQEDEINELNKKYTIQKVIELSVPELIGERHLIVLR

Sequences:

>Translated_203_residues
MKAKLVSLLAQANIKISDQQIQQLINLVNLLNKWNKAYNLTSVRDPQEMLVKHILDSLVVSPYLQGDRFIDVGTGPGLPG
LPLAIINPSKQFVLLDSLGKRISFIRNAIRELRLTNVTPVLSRVEEYQPEDKFDGVLSRAFASLKDMTDWCHHLPKENGY
FYALKGIYQEDEINELNKKYTIQKVIELSVPELIGERHLIVLR
>Mature_203_residues
MKAKLVSLLAQANIKISDQQIQQLINLVNLLNKWNKAYNLTSVRDPQEMLVKHILDSLVVSPYLQGDRFIDVGTGPGLPG
LPLAIINPSKQFVLLDSLGKRISFIRNAIRELRLTNVTPVLSRVEEYQPEDKFDGVLSRAFASLKDMTDWCHHLPKENGY
FYALKGIYQEDEINELNKKYTIQKVIELSVPELIGERHLIVLR

Specific function: Specifically methylates the N7 position of guanosine in position 527 of 16S rRNA

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family

Homologues:

Organism=Escherichia coli, GI1790179, Length=203, Percent_Identity=63.5467980295566, Blast_Score=272, Evalue=1e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMG_HAEI8 (Q4QN56)

Other databases:

- EMBL:   CP000057
- RefSeq:   YP_248201.1
- ProteinModelPortal:   Q4QN56
- SMR:   Q4QN56
- STRING:   Q4QN56
- GeneID:   3429928
- GenomeReviews:   CP000057_GR
- KEGG:   hit:NTHI0617
- eggNOG:   COG0357
- HOGENOM:   HBG686577
- OMA:   HIMDSIV
- PhylomeDB:   Q4QN56
- ProtClustDB:   PRK00107
- BioCyc:   HINF281310:NTHI0617-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00074
- InterPro:   IPR003682
- PIRSF:   PIRSF003078
- TIGRFAMs:   TIGR00138

Pfam domain/function: PF02527 GidB

EC number: =2.1.1.170

Molecular weight: Translated: 23178; Mature: 23178

Theoretical pI: Translated: 8.91; Mature: 8.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAKLVSLLAQANIKISDQQIQQLINLVNLLNKWNKAYNLTSVRDPQEMLVKHILDSLVV
CHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
SPYLQGDRFIDVGTGPGLPGLPLAIINPSKQFVLLDSLGKRISFIRNAIRELRLTNVTPV
HHCCCCCCEEECCCCCCCCCCCEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHCCHHHH
LSRVEEYQPEDKFDGVLSRAFASLKDMTDWCHHLPKENGYFYALKGIYQEDEINELNKKY
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHH
TIQKVIELSVPELIGERHLIVLR
HHHHHHHHHHHHHHCCCCEEEEC
>Mature Secondary Structure
MKAKLVSLLAQANIKISDQQIQQLINLVNLLNKWNKAYNLTSVRDPQEMLVKHILDSLVV
CHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
SPYLQGDRFIDVGTGPGLPGLPLAIINPSKQFVLLDSLGKRISFIRNAIRELRLTNVTPV
HHCCCCCCEEECCCCCCCCCCCEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHCCHHHH
LSRVEEYQPEDKFDGVLSRAFASLKDMTDWCHHLPKENGYFYALKGIYQEDEINELNKKY
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHH
TIQKVIELSVPELIGERHLIVLR
HHHHHHHHHHHHHHCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA