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The map label for this gene is ngoBVM [H]

Identifier: 148827577

GI number: 148827577

Start: 920369

End: 921643

Strand: Direct

Name: ngoBVM [H]

Synonym: CGSHiGG_05020

Alternate gene names: 148827577

Gene position: 920369-921643 (Clockwise)

Preceding gene: 148827576

Following gene: 148827578

Centisome position: 48.77

GC content: 46.98

Gene sequence:

>1275_bases
ATGTTTACCTACGGTTCAATCTGTTCAGGGATTGAAGCAGTAAGTGTGGCATGGAAAGGCTTAGGTAAGCCACTTTGGTT
TAGTGAAATTGAACCTTTTCCTTGTGCCGTGCTTACTTATCATTATCCCAACATCCCAAATCTTGGGGATATGACCACCT
TACCCGAAAAAATCTTAAACCGTGAAATTCCTGCGCCTGATGTGCTTGTTGGTGGTACACCTTGTCAAGCATTTTCGATA
GCTGGACTGCGAAACTCGCTAGATGACGAACGAGGAAATCTCACTTTAATGTTGATACACATATTAGAGGCTATTGACTA
TGTTAGATACCAAGACGGTAAACAGCCATGCATTTTGCTGTGGGAAAACGTACCAGGTGTCCTATCCACCAAGGACAACG
CATTCGGACACTTTCTGGCTGGATTGGCTCAAGAACGTGAGCCATTACAACCGGCAGGGGCAAGATGGGCAAACTCTGGT
TATGTGCATTCAGCCCGCACTATCGCGTGGCGAATCCTCGATGCTCAATACTTCGGAGTTGCCCAACGACGCCGTCGGGT
GTTTGTTGTGGCAAGTGCTAGACCGCGAAGTGTCGCCCAGATACTCATTGAGCGCAAAAGCTTGTGCGGGGATATTGAGA
CGGGCAAAAGCGCGGAAAAAAACATTACCGCCTACACTGAAAGTAGCTTTGGAACGTATATCCGATCCACAGTGGGGGGG
GTAGTAACTGCTAGTGGTGGTGCGCTTGGTGGCGGGTCGGAAATTCTTGTAGTACACGGCACGCAAGATCCGATAATATC
ATCCACCACCGCGCATTGCTTAGGTCGTAACGGCGGGCGAGAAAATATCTTATTTGATATTGCTCACCGCTCCGACGTAG
TACGCATACAAGATGATGATACTACGCCGACACTAACGGCGCGCATGGGGACTGGCGGTAATAATATCCCTTGCATTGCT
CTTGCTGGTAACACTATCGGCAGACAACCGCATAACGGCGGAAATGGCAATGGATTTGATGACAGCGGAGTAAGTTACAC
ATTAACAACTACAGATATTCACGGTGTTTTTAATGGCTTAACAGTCCGCAAACTTACACCTTCAGAATGCGAAAAACTAC
AGGGCTTTCCGCCAGGTTACACGCAAATCCCATATCGCAATAAACTTGCGAATGATTGCCCTGACAGTCCGCGCTATAAA
GCTATCGGCAATAGTATGGCTGTACCGGTTATTAAGTGGATCGGGGAAAGAATGATTAATTATTTAAACAAATAA

Upstream 100 bases:

>100_bases
GAAAACCGCAGTAGCCCATCAACAACAGCTACAACAGCAAAACCGATTGTGCCTGTGTTGCCTGTGATTAATCCAAAACA
GAGGAAAAAATAACCGCACT

Downstream 100 bases:

>100_bases
ATCCAATAGGCGTTCCAAGTGAGTGCCTTTTGTTTTAATGGAGATAGAAAATGAAAGAATTTAACTTAGATGCAGCTTTA
AATGGCGAGCCTGTTAAATT

Product: hypothetical protein

Products: NA

Alternate protein names: M.NgoBV; Cytosine-specific methyltransferase NgoBV; M.NgoV [H]

Number of amino acids: Translated: 424; Mature: 424

Protein sequence:

>424_residues
MFTYGSICSGIEAVSVAWKGLGKPLWFSEIEPFPCAVLTYHYPNIPNLGDMTTLPEKILNREIPAPDVLVGGTPCQAFSI
AGLRNSLDDERGNLTLMLIHILEAIDYVRYQDGKQPCILLWENVPGVLSTKDNAFGHFLAGLAQEREPLQPAGARWANSG
YVHSARTIAWRILDAQYFGVAQRRRRVFVVASARPRSVAQILIERKSLCGDIETGKSAEKNITAYTESSFGTYIRSTVGG
VVTASGGALGGGSEILVVHGTQDPIISSTTAHCLGRNGGRENILFDIAHRSDVVRIQDDDTTPTLTARMGTGGNNIPCIA
LAGNTIGRQPHNGGNGNGFDDSGVSYTLTTTDIHGVFNGLTVRKLTPSECEKLQGFPPGYTQIPYRNKLANDCPDSPRYK
AIGNSMAVPVIKWIGERMINYLNK

Sequences:

>Translated_424_residues
MFTYGSICSGIEAVSVAWKGLGKPLWFSEIEPFPCAVLTYHYPNIPNLGDMTTLPEKILNREIPAPDVLVGGTPCQAFSI
AGLRNSLDDERGNLTLMLIHILEAIDYVRYQDGKQPCILLWENVPGVLSTKDNAFGHFLAGLAQEREPLQPAGARWANSG
YVHSARTIAWRILDAQYFGVAQRRRRVFVVASARPRSVAQILIERKSLCGDIETGKSAEKNITAYTESSFGTYIRSTVGG
VVTASGGALGGGSEILVVHGTQDPIISSTTAHCLGRNGGRENILFDIAHRSDVVRIQDDDTTPTLTARMGTGGNNIPCIA
LAGNTIGRQPHNGGNGNGFDDSGVSYTLTTTDIHGVFNGLTVRKLTPSECEKLQGFPPGYTQIPYRNKLANDCPDSPRYK
AIGNSMAVPVIKWIGERMINYLNK
>Mature_424_residues
MFTYGSICSGIEAVSVAWKGLGKPLWFSEIEPFPCAVLTYHYPNIPNLGDMTTLPEKILNREIPAPDVLVGGTPCQAFSI
AGLRNSLDDERGNLTLMLIHILEAIDYVRYQDGKQPCILLWENVPGVLSTKDNAFGHFLAGLAQEREPLQPAGARWANSG
YVHSARTIAWRILDAQYFGVAQRRRRVFVVASARPRSVAQILIERKSLCGDIETGKSAEKNITAYTESSFGTYIRSTVGG
VVTASGGALGGGSEILVVHGTQDPIISSTTAHCLGRNGGRENILFDIAHRSDVVRIQDDDTTPTLTARMGTGGNNIPCIA
LAGNTIGRQPHNGGNGNGFDDSGVSYTLTTTDIHGVFNGLTVRKLTPSECEKLQGFPPGYTQIPYRNKLANDCPDSPRYK
AIGNSMAVPVIKWIGERMINYLNK

Specific function: This methylase recognizes the double-stranded sequence GGNNCC, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the NgoBV endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 45967; Mature: 45967

Theoretical pI: Translated: 7.73; Mature: 7.73

Prosite motif: PS00094 C5_MTASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTYGSICSGIEAVSVAWKGLGKPLWFSEIEPFPCAVLTYHYPNIPNLGDMTTLPEKILN
CCCCHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHC
REIPAPDVLVGGTPCQAFSIAGLRNSLDDERGNLTLMLIHILEAIDYVRYQDGKQPCILL
CCCCCCCEEECCCCCCCEEHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHCCCCCCEEEE
WENVPGVLSTKDNAFGHFLAGLAQEREPLQPAGARWANSGYVHSARTIAWRILDAQYFGV
ECCCCCCEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEHHHEEEEEEECHHHHHH
AQRRRRVFVVASARPRSVAQILIERKSLCGDIETGKSAEKNITAYTESSFGTYIRSTVGG
HHHCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCHHHHHHHHHCC
VVTASGGALGGGSEILVVHGTQDPIISSTTAHCLGRNGGRENILFDIAHRSDVVRIQDDD
EEEECCCCCCCCCEEEEEECCCCCCCCCCHHHHHCCCCCCCCEEEEEECCCCEEEEECCC
TTPTLTARMGTGGNNIPCIALAGNTIGRQPHNGGNGNGFDDSGVSYTLTTTDIHGVFNGL
CCCEEEEEECCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHHHCCE
TVRKLTPSECEKLQGFPPGYTQIPYRNKLANDCPDSPRYKAIGNSMAVPVIKWIGERMIN
EEECCCHHHHHHHCCCCCCCCCCCCCHHHHHCCCCCCCHHHCCCCCHHHHHHHHHHHHHH
YLNK
HHCC
>Mature Secondary Structure
MFTYGSICSGIEAVSVAWKGLGKPLWFSEIEPFPCAVLTYHYPNIPNLGDMTTLPEKILN
CCCCHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHC
REIPAPDVLVGGTPCQAFSIAGLRNSLDDERGNLTLMLIHILEAIDYVRYQDGKQPCILL
CCCCCCCEEECCCCCCCEEHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHCCCCCCEEEE
WENVPGVLSTKDNAFGHFLAGLAQEREPLQPAGARWANSGYVHSARTIAWRILDAQYFGV
ECCCCCCEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEHHHEEEEEEECHHHHHH
AQRRRRVFVVASARPRSVAQILIERKSLCGDIETGKSAEKNITAYTESSFGTYIRSTVGG
HHHCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCHHHHHHHHHCC
VVTASGGALGGGSEILVVHGTQDPIISSTTAHCLGRNGGRENILFDIAHRSDVVRIQDDD
EEEECCCCCCCCCEEEEEECCCCCCCCCCHHHHHCCCCCCCCEEEEEECCCCEEEEECCC
TTPTLTARMGTGGNNIPCIALAGNTIGRQPHNGGNGNGFDDSGVSYTLTTTDIHGVFNGL
CCCEEEEEECCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHHHCCE
TVRKLTPSECEKLQGFPPGYTQIPYRNKLANDCPDSPRYKAIGNSMAVPVIKWIGERMIN
EEECCCHHHHHHHCCCCCCCCCCCCCHHHHHCCCCCCCHHHCCCCCHHHHHHHHHHHHHH
YLNK
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7607490 [H]