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The map label for this gene is ispG [H]

Identifier: 148827507

GI number: 148827507

Start: 869591

End: 870697

Strand: Direct

Name: ispG [H]

Synonym: CGSHiGG_04650

Alternate gene names: 148827507

Gene position: 869591-870697 (Clockwise)

Preceding gene: 148827506

Following gene: 148827508

Centisome position: 46.08

GC content: 41.92

Gene sequence:

>1107_bases
ATGTCAGCTTTTCAGCCAACTATTAAGCGTCGTGAATCGACAAAAATTTATGTGGGAAATGTACCAATTGGTGGGGATGC
ACCGATTGCCGTGCAATCAATGACAAATACTCGCACCACTGATGTGGAAGCGACAGTTGCTCAAATTAAATCATTAGAAC
GTGTTGGTGCAGATATTGTTCGTGTATCTGTTCCAACAATGGATGCTGCGGAAGCATTTAAACAAATTAAACAACAAGTG
AATGTCCCGCTCGTAGCAGATATTCATTTTGACTATCGTATCGCGTTAAAAGTCGCAGAATATGGAGTGGATTGTTTACG
TATCAATCCTGGCAACATTGGTCGTGAAGATCGCGTCCGTGCCGTTGTTGATTGTGCGCGAGACAAAAATATTCCGATTC
GTATTGGCGTAAATGCAGGCTCTTTAGAAAAAGATTTGCAAGAAAAATATGGCGAACCAACGCCAGAAGCCTTGTTAGAA
TCCGCATTGCGTCATGTAGAAATTCTAGATCGTCTTAACTTCGATCAGTTTAAAGTGAGCGTAAAAGCCTCCGATGTATT
CTTAGCGGTTGAATCTTATCGTTTACTGGCTAAAGCAATTAAACAGCCTTTACATTTAGGCATTACAGAAGCAGGTGGTG
CACGCGCTGGCGCAGTAAAATCTGCGGTGGGTTTAGGAATGTTATTAGCTGAGGGCATTGGCGATACACTGCGCATCTCT
TTGGCGGCAGATCCCATAGAGGAAATCAAAGTCGGTTTTGATATTTTGAAATCTTTACGGATTCGTTCAAGAGGAATTAA
CTTTATTGCTTGCCCAACCTGTTCTCGCCAAGAATTTGATGTAATCGGTACAGTAAATGCGCTAGAACAACGCCTTGAAG
ATATTATTACACCAATGGATGTATCTATTATCGGTTGTGTAGTGAATGGTCCTGGCGAGGCACTCATCTCCGATCTGGGC
GTAACGGGCGGTAACAAAAAAAGCGGTTATTATCTTGACGGCGAACGCCAAAAAGAGCGTTTTGATAACGAAGATATAGT
GAACCAATTAGAAGCAAAAATTCGTGCGAAAGTCGCACGACAAGATCCAAAAAACAGAATTATTTAA

Upstream 100 bases:

>100_bases
CACCAAGCAATGTGCGTATCACTTACAAAGGCGAGAATTATCCTCTCAAAGTGGATGGTCGCGTAGCAAAATTCAAGCTT
TCACAACCTTAGGAAAAATA

Downstream 100 bases:

>100_bases
GGAAAGCGCGTGGCAAAAACAATTCAAGCAATTCGTGGTATGAACGATTGCGCCCCAACAGAATCTCCATTATGGCAATG
GATTGAAGCACAAGTACGCA

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase [H]

Number of amino acids: Translated: 368; Mature: 367

Protein sequence:

>368_residues
MSAFQPTIKRRESTKIYVGNVPIGGDAPIAVQSMTNTRTTDVEATVAQIKSLERVGADIVRVSVPTMDAAEAFKQIKQQV
NVPLVADIHFDYRIALKVAEYGVDCLRINPGNIGREDRVRAVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPEALLE
SALRHVEILDRLNFDQFKVSVKASDVFLAVESYRLLAKAIKQPLHLGITEAGGARAGAVKSAVGLGMLLAEGIGDTLRIS
LAADPIEEIKVGFDILKSLRIRSRGINFIACPTCSRQEFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALISDLG
VTGGNKKSGYYLDGERQKERFDNEDIVNQLEAKIRAKVARQDPKNRII

Sequences:

>Translated_368_residues
MSAFQPTIKRRESTKIYVGNVPIGGDAPIAVQSMTNTRTTDVEATVAQIKSLERVGADIVRVSVPTMDAAEAFKQIKQQV
NVPLVADIHFDYRIALKVAEYGVDCLRINPGNIGREDRVRAVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPEALLE
SALRHVEILDRLNFDQFKVSVKASDVFLAVESYRLLAKAIKQPLHLGITEAGGARAGAVKSAVGLGMLLAEGIGDTLRIS
LAADPIEEIKVGFDILKSLRIRSRGINFIACPTCSRQEFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALISDLG
VTGGNKKSGYYLDGERQKERFDNEDIVNQLEAKIRAKVARQDPKNRII
>Mature_367_residues
SAFQPTIKRRESTKIYVGNVPIGGDAPIAVQSMTNTRTTDVEATVAQIKSLERVGADIVRVSVPTMDAAEAFKQIKQQVN
VPLVADIHFDYRIALKVAEYGVDCLRINPGNIGREDRVRAVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPEALLES
ALRHVEILDRLNFDQFKVSVKASDVFLAVESYRLLAKAIKQPLHLGITEAGGARAGAVKSAVGLGMLLAEGIGDTLRISL
AADPIEEIKVGFDILKSLRIRSRGINFIACPTCSRQEFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALISDLGV
TGGNKKSGYYLDGERQKERFDNEDIVNQLEAKIRAKVARQDPKNRII

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate [H]

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family [H]

Homologues:

Organism=Escherichia coli, GI1788863, Length=363, Percent_Identity=87.3278236914601, Blast_Score=643, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR011005
- InterPro:   IPR016425
- InterPro:   IPR004588 [H]

Pfam domain/function: PF04551 GcpE [H]

EC number: =1.17.7.1 [H]

Molecular weight: Translated: 40159; Mature: 40028

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAFQPTIKRRESTKIYVGNVPIGGDAPIAVQSMTNTRTTDVEATVAQIKSLERVGADIV
CCCCCCHHHCCCCCEEEECCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCEE
RVSVPTMDAAEAFKQIKQQVNVPLVADIHFDYRIALKVAEYGVDCLRINPGNIGREDRVR
EEECCCCHHHHHHHHHHHHCCCCEEEEECCCEEEEEEEHHCCCEEEEECCCCCCHHHHHH
AVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPEALLESALRHVEILDRLNFDQFKVS
HHHHHHCCCCCCEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEE
VKASDVFLAVESYRLLAKAIKQPLHLGITEAGGARAGAVKSAVGLGMLLAEGIGDTLRIS
EEECEEEEEHHHHHHHHHHHHCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEE
LAADPIEEIKVGFDILKSLRIRSRGINFIACPTCSRQEFDVIGTVNALEQRLEDIITPMD
EECCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHEECHHHHHHHHHHHCCCCC
VSIIGCVVNGPGEALISDLGVTGGNKKSGYYLDGERQKERFDNEDIVNQLEAKIRAKVAR
EEEEEEEECCCHHHHHHHHCCCCCCCCCCEEECCCHHHHHCCCHHHHHHHHHHHHHHHHH
QDPKNRII
CCCCCCCC
>Mature Secondary Structure 
SAFQPTIKRRESTKIYVGNVPIGGDAPIAVQSMTNTRTTDVEATVAQIKSLERVGADIV
CCCCCHHHCCCCCEEEECCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCEE
RVSVPTMDAAEAFKQIKQQVNVPLVADIHFDYRIALKVAEYGVDCLRINPGNIGREDRVR
EEECCCCHHHHHHHHHHHHCCCCEEEEECCCEEEEEEEHHCCCEEEEECCCCCCHHHHHH
AVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPEALLESALRHVEILDRLNFDQFKVS
HHHHHHCCCCCCEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEE
VKASDVFLAVESYRLLAKAIKQPLHLGITEAGGARAGAVKSAVGLGMLLAEGIGDTLRIS
EEECEEEEEHHHHHHHHHHHHCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEE
LAADPIEEIKVGFDILKSLRIRSRGINFIACPTCSRQEFDVIGTVNALEQRLEDIITPMD
EECCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHEECHHHHHHHHHHHCCCCC
VSIIGCVVNGPGEALISDLGVTGGNKKSGYYLDGERQKERFDNEDIVNQLEAKIRAKVAR
EEEEEEEECCCHHHHHHHHCCCCCCCCCCEEECCCHHHHHCCCHHHHHHHHHHHHHHHHH
QDPKNRII
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA