Click here to switch to the map view.

The map label for this gene is trmB [H]

Identifier: 148827486

GI number: 148827486

Start: 846275

End: 847015

Strand: Direct

Name: trmB [H]

Synonym: CGSHiGG_04505

Alternate gene names: 148827486

Gene position: 846275-847015 (Clockwise)

Preceding gene: 148827485

Following gene: 148827487

Centisome position: 44.84

GC content: 38.6

Gene sequence:

>741_bases
ATGACACAAACTTTTGCTGATCAAAAACGTAAAACCGTTGAAACCGCAGAATTTACCGAAGATGGCCGCTATAAGCGCAA
AGTCCGTAGTTTTGTTCTCCGCACTGGTCGTTTGAGCGAATTTCAAAGAAATATGATGAATGATAACTGGGGAACACTTG
GTTTAGATTATCAAACGGAACCTTTTGATTTTGCTAAGATTTATGGCAATGATAATCCAGTTGTATTAGAAATTGGCTTT
GGAATGGGAAAATCGCTCGTGGATATGGCTTTTGCTAATCCTGACAAAAATTACCTTGGCATTGAAGTTCATACTCCTGG
CGTTGGTGCTTGTATTGCTTATGCAGTGGAAAAAGGCGTAACGAATTTACGCGTGATCTGTCATGATGCAACGGAAATTT
TACGTGACAGTATTGCTGATGGCACGCTTGGCGGTTTGCAGTTATTTTTCCCCGATCCTTGGCATAAAGCAAAGCATCAT
AAACGTCGTATTGTTCAACCGCACTTTGTGACACAAGTTATACAAAAATTAAGTGAAAATGGTTTTATCCATATGGCGAC
AGACTGGGAAAATTATGCAGAGCAAATGCTAGAAGTATTAAGCGCAAACACCGATTTAGTGAACACTTCGAAAAATGGTA
ATTATATTCCAAGACCAGATTTTAGACCTTTAACTAAATTTGAAGCCAGAGGTCACAAACTTGGACACGGTGTTTGGGAT
TTATATTTTGTGAAGAAATAA

Upstream 100 bases:

>100_bases
CTCGGACAATTTTCATAATCAACCTTGGGAAAAAATTTGCCCTATTATACGAGAAAAATTGGTATAATCGCCGCCAATTT
TTTTCACGAACAAGATTTTT

Downstream 100 bases:

>100_bases
ATCAGTTTTAGGCAAACTCGATGCCTAATTGATGAATTTAATATAGAATATGCCTTCAAATATAACCTTAATAGGAGAAA
TACAATGTCTAAATCTTACA

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 246; Mature: 245

Protein sequence:

>246_residues
MTQTFADQKRKTVETAEFTEDGRYKRKVRSFVLRTGRLSEFQRNMMNDNWGTLGLDYQTEPFDFAKIYGNDNPVVLEIGF
GMGKSLVDMAFANPDKNYLGIEVHTPGVGACIAYAVEKGVTNLRVICHDATEILRDSIADGTLGGLQLFFPDPWHKAKHH
KRRIVQPHFVTQVIQKLSENGFIHMATDWENYAEQMLEVLSANTDLVNTSKNGNYIPRPDFRPLTKFEARGHKLGHGVWD
LYFVKK

Sequences:

>Translated_246_residues
MTQTFADQKRKTVETAEFTEDGRYKRKVRSFVLRTGRLSEFQRNMMNDNWGTLGLDYQTEPFDFAKIYGNDNPVVLEIGF
GMGKSLVDMAFANPDKNYLGIEVHTPGVGACIAYAVEKGVTNLRVICHDATEILRDSIADGTLGGLQLFFPDPWHKAKHH
KRRIVQPHFVTQVIQKLSENGFIHMATDWENYAEQMLEVLSANTDLVNTSKNGNYIPRPDFRPLTKFEARGHKLGHGVWD
LYFVKK
>Mature_245_residues
TQTFADQKRKTVETAEFTEDGRYKRKVRSFVLRTGRLSEFQRNMMNDNWGTLGLDYQTEPFDFAKIYGNDNPVVLEIGFG
MGKSLVDMAFANPDKNYLGIEVHTPGVGACIAYAVEKGVTNLRVICHDATEILRDSIADGTLGGLQLFFPDPWHKAKHHK
RRIVQPHFVTQVIQKLSENGFIHMATDWENYAEQMLEVLSANTDLVNTSKNGNYIPRPDFRPLTKFEARGHKLGHGVWDL
YFVKK

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Escherichia coli, GI1789330, Length=234, Percent_Identity=54.7008547008547, Blast_Score=278, Evalue=2e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4 [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 28086; Mature: 27955

Theoretical pI: Translated: 8.24; Mature: 8.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQTFADQKRKTVETAEFTEDGRYKRKVRSFVLRTGRLSEFQRNMMNDNWGTLGLDYQTE
CCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCEEECCCCCC
PFDFAKIYGNDNPVVLEIGFGMGKSLVDMAFANPDKNYLGIEVHTPGVGACIAYAVEKGV
CCCEEEEECCCCCEEEEECCCCCHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHCC
TNLRVICHDATEILRDSIADGTLGGLQLFFPDPWHKAKHHKRRIVQPHFVTQVIQKLSEN
CEEEEEEHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHCC
GFIHMATDWENYAEQMLEVLSANTDLVNTSKNGNYIPRPDFRPLTKFEARGHKLGHGVWD
CEEEEECCHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCHHHHHCCCCCCCCEEE
LYFVKK
EEEECC
>Mature Secondary Structure 
TQTFADQKRKTVETAEFTEDGRYKRKVRSFVLRTGRLSEFQRNMMNDNWGTLGLDYQTE
CCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCEEECCCCCC
PFDFAKIYGNDNPVVLEIGFGMGKSLVDMAFANPDKNYLGIEVHTPGVGACIAYAVEKGV
CCCEEEEECCCCCEEEEECCCCCHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHCC
TNLRVICHDATEILRDSIADGTLGGLQLFFPDPWHKAKHHKRRIVQPHFVTQVIQKLSEN
CEEEEEEHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHCC
GFIHMATDWENYAEQMLEVLSANTDLVNTSKNGNYIPRPDFRPLTKFEARGHKLGHGVWD
CEEEEECCHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCHHHHHCCCCCCCCEEE
LYFVKK
EEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA