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The map label for this gene is pcnB [H]

Identifier: 148827216

GI number: 148827216

Start: 520666

End: 522024

Strand: Direct

Name: pcnB [H]

Synonym: CGSHiGG_02830

Alternate gene names: 148827216

Gene position: 520666-522024 (Clockwise)

Preceding gene: 148827215

Following gene: 148827217

Centisome position: 27.59

GC content: 41.43

Gene sequence:

>1359_bases
ATGCCAAAAGCGCGAGCAAAAAAATCAGAACAAACACGTCGCTATGATAAAAATGTGATTAAAGCGGCTCAATTTGATAT
TTCTCCACGTGATTTTAGCCGTAATGCTCTCAATGTAGTGGAAAAACTGCAACGTCAAGGTTTCGAGGCTTATATCGTCG
GGGGATGTATCCGTGATTTATTGCTCGGTAAAAAACCAAAAGATTTTGATGTTGCAACAAACGCCCGTCCTGAGCAAATC
CAAAACATTTTTCAACGCCAATGTCGTTTAGTCGGTCGCCGTTTCCGATTAGCACATATTATGTTTGGTCGAGATATTAT
TGAAGTCGCCACTTTCCGCGCTAATCACAGTGATGCTCGCAATGAAAATCAAGCGAAACAGAGCAATGAAGGCATGTTAC
TGCGCGATAATGTTTACGGTACGATCGAACAAGATGCAGCACGCCGTGATTTTACGGTCAATGCGCTGTATTACAATCCG
CAAGATAATACGTTACGAGATTATTTCGAAGGCATTAAGGATCTCAAAGCGGGCAAGCTACGCTTAATTGGCGATCCTGT
TACACGTTATCAAGAAGATCCTGTGCGCATGCTACGCTCAATTCGTTTTATGGCAAAACTTGATATGTTCCTTGAAAAAC
CAAGTGAACAGCCAATTCGTGAACTTGCGCCTTTATTAAAAAATATTCCGCCAGCTCGCTTATTTGACGAAAGTTTAAAA
TTATTGCAGGCAGGACAAGGGGTAAAAACCTATCGGTTATTACGCCAATATGGTTTATTTGAACAACTTTTCCCTACATT
AAGCGCATATTTTACAGAGAAAGAAGATAGCTTTGCGGAACGTATGATCGTTACCGCCCTTAGTTCGACAGATGAACGTG
TTGCGGATAAACTTCGTATTAATCCTGCATTCTTATTTGCAGCATTTTTCTGGTATCCATTGCGCGAAAAAGTGGAAATC
TTGAAAAATGAAGGTGGTTTAAATAATCATGATGCTTATGCGTTGGCTGGCAATGAAGTTTTAGATTTATTCTGCCGTGC
TTTGGCTGCACCACGTCGTCATACTGCGGTCATTCGTGACATTTGGTTCTTACAACTGCAATTATTAAAACGTACTGGAT
CAGCACCAATGCGTACCATGGAACATCCAAAATTCCGTGCTGGCTTTGATTTATTGGCTATGCGAGCAGAAATTGAAGGT
GGCGAAACCATTGAATTAGCCAAATGGTGGCACGAATATCAATTCAGCAATGGTGAACAGCGCGAACAGTTGATACAAGA
GCAACAACGCTTACATCCTAAACCAAAGAAAAAATACTACCGTCCACGCCGTCGTAAAACAACGCGCTCAGCTGAATAA

Upstream 100 bases:

>100_bases
TACTTATATCAAAAATTTGTTTGGCAAGAAGACAAAAAAATCAAAAGAGGTTGCCAAAACAAGTACTCATTCTAAACGAG
TTGAGCGTTCTAATAGCCAA

Downstream 100 bases:

>100_bases
GGAAAAAGATGATTACTGCATATATTGCGCTGGGTAGCAATTTAAATACGCCAGTAGAACAATTACATGCTGCGCTTAAA
GCGATAAGTCAGTTACCAAA

Product: polyA polymerase

Products: NA

Alternate protein names: PAP [H]

Number of amino acids: Translated: 452; Mature: 451

Protein sequence:

>452_residues
MPKARAKKSEQTRRYDKNVIKAAQFDISPRDFSRNALNVVEKLQRQGFEAYIVGGCIRDLLLGKKPKDFDVATNARPEQI
QNIFQRQCRLVGRRFRLAHIMFGRDIIEVATFRANHSDARNENQAKQSNEGMLLRDNVYGTIEQDAARRDFTVNALYYNP
QDNTLRDYFEGIKDLKAGKLRLIGDPVTRYQEDPVRMLRSIRFMAKLDMFLEKPSEQPIRELAPLLKNIPPARLFDESLK
LLQAGQGVKTYRLLRQYGLFEQLFPTLSAYFTEKEDSFAERMIVTALSSTDERVADKLRINPAFLFAAFFWYPLREKVEI
LKNEGGLNNHDAYALAGNEVLDLFCRALAAPRRHTAVIRDIWFLQLQLLKRTGSAPMRTMEHPKFRAGFDLLAMRAEIEG
GETIELAKWWHEYQFSNGEQREQLIQEQQRLHPKPKKKYYRPRRRKTTRSAE

Sequences:

>Translated_452_residues
MPKARAKKSEQTRRYDKNVIKAAQFDISPRDFSRNALNVVEKLQRQGFEAYIVGGCIRDLLLGKKPKDFDVATNARPEQI
QNIFQRQCRLVGRRFRLAHIMFGRDIIEVATFRANHSDARNENQAKQSNEGMLLRDNVYGTIEQDAARRDFTVNALYYNP
QDNTLRDYFEGIKDLKAGKLRLIGDPVTRYQEDPVRMLRSIRFMAKLDMFLEKPSEQPIRELAPLLKNIPPARLFDESLK
LLQAGQGVKTYRLLRQYGLFEQLFPTLSAYFTEKEDSFAERMIVTALSSTDERVADKLRINPAFLFAAFFWYPLREKVEI
LKNEGGLNNHDAYALAGNEVLDLFCRALAAPRRHTAVIRDIWFLQLQLLKRTGSAPMRTMEHPKFRAGFDLLAMRAEIEG
GETIELAKWWHEYQFSNGEQREQLIQEQQRLHPKPKKKYYRPRRRKTTRSAE
>Mature_451_residues
PKARAKKSEQTRRYDKNVIKAAQFDISPRDFSRNALNVVEKLQRQGFEAYIVGGCIRDLLLGKKPKDFDVATNARPEQIQ
NIFQRQCRLVGRRFRLAHIMFGRDIIEVATFRANHSDARNENQAKQSNEGMLLRDNVYGTIEQDAARRDFTVNALYYNPQ
DNTLRDYFEGIKDLKAGKLRLIGDPVTRYQEDPVRMLRSIRFMAKLDMFLEKPSEQPIRELAPLLKNIPPARLFDESLKL
LQAGQGVKTYRLLRQYGLFEQLFPTLSAYFTEKEDSFAERMIVTALSSTDERVADKLRINPAFLFAAFFWYPLREKVEIL
KNEGGLNNHDAYALAGNEVLDLFCRALAAPRRHTAVIRDIWFLQLQLLKRTGSAPMRTMEHPKFRAGFDLLAMRAEIEGG
ETIELAKWWHEYQFSNGEQREQLIQEQQRLHPKPKKKYYRPRRRKTTRSAE

Specific function: Polymerase that creates the 3' poly(A) tail found in some mRNA's [H]

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=222, Percent_Identity=31.5315315315315, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI87081691, Length=412, Percent_Identity=56.5533980582524, Blast_Score=477, Evalue=1e-136,
Organism=Escherichia coli, GI1789436, Length=227, Percent_Identity=28.6343612334802, Blast_Score=73, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI71995920, Length=324, Percent_Identity=27.7777777777778, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI281360112, Length=242, Percent_Identity=27.6859504132231, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI21357337, Length=242, Percent_Identity=27.6859504132231, Blast_Score=89, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002646
- InterPro:   IPR010206 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.19 [H]

Molecular weight: Translated: 52767; Mature: 52636

Theoretical pI: Translated: 10.27; Mature: 10.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKARAKKSEQTRRYDKNVIKAAQFDISPRDFSRNALNVVEKLQRQGFEAYIVGGCIRDL
CCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHCCCCEEEHHHHHHHH
LLGKKPKDFDVATNARPEQIQNIFQRQCRLVGRRFRLAHIMFGRDIIEVATFRANHSDAR
HHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
NENQAKQSNEGMLLRDNVYGTIEQDAARRDFTVNALYYNPQDNTLRDYFEGIKDLKAGKL
CHHHHHHCCCCEEEECCCCCCHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHCCCCE
RLIGDPVTRYQEDPVRMLRSIRFMAKLDMFLEKPSEQPIRELAPLLKNIPPARLFDESLK
EEECCHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHH
LLQAGQGVKTYRLLRQYGLFEQLFPTLSAYFTEKEDSFAERMIVTALSSTDERVADKLRI
HHHCCCCHHHHHHHHHHCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHCC
NPAFLFAAFFWYPLREKVEILKNEGGLNNHDAYALAGNEVLDLFCRALAAPRRHTAVIRD
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCHHHHHHHHHHHCCCHHHHHHHHH
IWFLQLQLLKRTGSAPMRTMEHPKFRAGFDLLAMRAEIEGGETIELAKWWHEYQFSNGEQ
HHHHHHHHHHHCCCCCHHHHCCCCHHHCHHHHHHHHHCCCCCEEHHHHHHHHHHCCCCHH
REQLIQEQQRLHPKPKKKYYRPRRRKTTRSAE
HHHHHHHHHHCCCCCHHHHCCCHHHCCCCCCC
>Mature Secondary Structure 
PKARAKKSEQTRRYDKNVIKAAQFDISPRDFSRNALNVVEKLQRQGFEAYIVGGCIRDL
CCCCCHHHHHHHHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHCCCCEEEHHHHHHHH
LLGKKPKDFDVATNARPEQIQNIFQRQCRLVGRRFRLAHIMFGRDIIEVATFRANHSDAR
HHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
NENQAKQSNEGMLLRDNVYGTIEQDAARRDFTVNALYYNPQDNTLRDYFEGIKDLKAGKL
CHHHHHHCCCCEEEECCCCCCHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHCCCCE
RLIGDPVTRYQEDPVRMLRSIRFMAKLDMFLEKPSEQPIRELAPLLKNIPPARLFDESLK
EEECCHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHH
LLQAGQGVKTYRLLRQYGLFEQLFPTLSAYFTEKEDSFAERMIVTALSSTDERVADKLRI
HHHCCCCHHHHHHHHHHCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHCC
NPAFLFAAFFWYPLREKVEILKNEGGLNNHDAYALAGNEVLDLFCRALAAPRRHTAVIRD
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCHHHHHHHHHHHCCCHHHHHHHHH
IWFLQLQLLKRTGSAPMRTMEHPKFRAGFDLLAMRAEIEGGETIELAKWWHEYQFSNGEQ
HHHHHHHHHHHCCCCCHHHHCCCCHHHCHHHHHHHHHCCCCCEEHHHHHHHHHHCCCCHH
REQLIQEQQRLHPKPKKKYYRPRRRKTTRSAE
HHHHHHHHHHCCCCCHHHHCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]