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The map label for this gene is miaB [H]

Identifier: 148827177

GI number: 148827177

Start: 472060

End: 473484

Strand: Reverse

Name: miaB [H]

Synonym: CGSHiGG_02565

Alternate gene names: 148827177

Gene position: 473484-472060 (Counterclockwise)

Preceding gene: 148827178

Following gene: 148827176

Centisome position: 25.09

GC content: 41.54

Gene sequence:

>1425_bases
ATGACTCAAAAATTACATATTAAAACTTGGGGCTGCCAGATGAATGAATATGATTCATCTAAAATGGCTGATCTTTTATT
AAGTACCCACGGATTAGAACTCACGGAAGCACCTGAAGAAGCAGACGTGTTATTATTAAATACCTGTTCTATTCGTGAAA
AAGCACAAGAAAAAGTGTTCCACCAACTTGGGCGTTGGAAAGAATTAAAGAAAAATAATCCAAATTTAGTGATTGGTGTG
GGCGGCTGTGTAGCCTCTCAAGAAGGTGAACATATTCGCCATCGTGCGCCTTATGTGGATATTATTTTTGGCCCGCAAAC
TTTGCATCGCTTGCCTGAAATGATTAATCAAATTCGTGGTGGAAAAAGTTCCGTCGTAGACGTAAGTTTCCCTGAAATTG
AGAAATTTGACCGCTTACCTGAACCTCGCGCAGAAGGCCCAACGGCATTTGTTTCCATTATGGAAGGCTGTAATAAATAC
TGTACTTTTTGTGTTGTGCCTTATACTCGTGGAGAAGAAGTTAGCCGTCCCGTAGATGATGTGCTATTTGAAATTGCGCA
ACTTGCGGAACAAGGTGTGCGTGAGGTAAATCTACTTGGTCAAAATGTAAATGCTTATCGTGGGCCAACGCATGATGGTC
AAATTTGCAGCTTTGCTGAGTTGCTTCGTCTTGTGGCGTCTATCGATGGTATTGACCGTTTACGTTTCACAACCAGCCAT
CCAATAGAATTTACCGATGATATTATTGATGTATATCGTGATACGCCTGAATTGGTGAGTTTCCTACATCTTCCAGTGCA
AGCAGGTTCTGACCGTGTATTAACAATGATGAAACGTGCTCATACCGCATTAGAATATAAATCCATTATTCGTAAACTTC
GTGCAGTGCGCCCAGATATTCAAATCAGCTCTGACTTTATTGTTGGTTTCCCAGGGGAAACGGCGGAAGATTTTGAGCAA
ACTATGAATTTGATTGCACAAGTAAACTTTGATATGAGCTTCAGTTTTGTTTATTCCGCTCGACCAGGTACGCCAGCAGC
AGATATGCCAGATGATGTGACGGAAGATGAAAAGAAACAGCGTCTTTATGTGCTACAAGAGCGTATTAATCAACAAGCGG
CACAGTTTAGCCGTCGTATGCTTGGCACAGAGCAGCGCGTGTTAGTTGAAGGGCCATCGAAGAAAGATATTATGGAATTA
ACAGGACGCACTGAAACAAATCGTATTGTTAATTTCCAAGGTTCTCCAGAAATGATCGGTAAGTTTGTGGATGTGAAAAT
CACTGATGTTTATACTAATTCGTTACGTGGCGAAGTGGTGCGTACTGAAGATGAAATGGGATTACGTATTGCGCAATCTC
CACAAGAAGTGATGAATCGCACACGTAAAGAAGATGAATTAGGCGTAGGGCGTTATCACGGCTAA

Upstream 100 bases:

>100_bases
TTTCACCGAATATTCACTAGCCAGAATTAGCGCAAGCCTGTAGAATAGCCCATTGCTTTATTTAACTAATTATTTATAAC
ACCTAACGATATTGTAGAAA

Downstream 100 bases:

>100_bases
TTAAAATACTGAAATATTTAACCGCACTTTGAGAGATTAAAGTGCGGTTATTTTTTTAGAGATATGGTTTAGATTTGCCA
ATCAATTGGTTTCATTCCAT

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase [H]

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MTQKLHIKTWGCQMNEYDSSKMADLLLSTHGLELTEAPEEADVLLLNTCSIREKAQEKVFHQLGRWKELKKNNPNLVIGV
GGCVASQEGEHIRHRAPYVDIIFGPQTLHRLPEMINQIRGGKSSVVDVSFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
CTFCVVPYTRGEEVSRPVDDVLFEIAQLAEQGVREVNLLGQNVNAYRGPTHDGQICSFAELLRLVASIDGIDRLRFTTSH
PIEFTDDIIDVYRDTPELVSFLHLPVQAGSDRVLTMMKRAHTALEYKSIIRKLRAVRPDIQISSDFIVGFPGETAEDFEQ
TMNLIAQVNFDMSFSFVYSARPGTPAADMPDDVTEDEKKQRLYVLQERINQQAAQFSRRMLGTEQRVLVEGPSKKDIMEL
TGRTETNRIVNFQGSPEMIGKFVDVKITDVYTNSLRGEVVRTEDEMGLRIAQSPQEVMNRTRKEDELGVGRYHG

Sequences:

>Translated_474_residues
MTQKLHIKTWGCQMNEYDSSKMADLLLSTHGLELTEAPEEADVLLLNTCSIREKAQEKVFHQLGRWKELKKNNPNLVIGV
GGCVASQEGEHIRHRAPYVDIIFGPQTLHRLPEMINQIRGGKSSVVDVSFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
CTFCVVPYTRGEEVSRPVDDVLFEIAQLAEQGVREVNLLGQNVNAYRGPTHDGQICSFAELLRLVASIDGIDRLRFTTSH
PIEFTDDIIDVYRDTPELVSFLHLPVQAGSDRVLTMMKRAHTALEYKSIIRKLRAVRPDIQISSDFIVGFPGETAEDFEQ
TMNLIAQVNFDMSFSFVYSARPGTPAADMPDDVTEDEKKQRLYVLQERINQQAAQFSRRMLGTEQRVLVEGPSKKDIMEL
TGRTETNRIVNFQGSPEMIGKFVDVKITDVYTNSLRGEVVRTEDEMGLRIAQSPQEVMNRTRKEDELGVGRYHG
>Mature_473_residues
TQKLHIKTWGCQMNEYDSSKMADLLLSTHGLELTEAPEEADVLLLNTCSIREKAQEKVFHQLGRWKELKKNNPNLVIGVG
GCVASQEGEHIRHRAPYVDIIFGPQTLHRLPEMINQIRGGKSSVVDVSFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKYC
TFCVVPYTRGEEVSRPVDDVLFEIAQLAEQGVREVNLLGQNVNAYRGPTHDGQICSFAELLRLVASIDGIDRLRFTTSHP
IEFTDDIIDVYRDTPELVSFLHLPVQAGSDRVLTMMKRAHTALEYKSIIRKLRAVRPDIQISSDFIVGFPGETAEDFEQT
MNLIAQVNFDMSFSFVYSARPGTPAADMPDDVTEDEKKQRLYVLQERINQQAAQFSRRMLGTEQRVLVEGPSKKDIMELT
GRTETNRIVNFQGSPEMIGKFVDVKITDVYTNSLRGEVVRTEDEMGLRIAQSPQEVMNRTRKEDELGVGRYHG

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872784, Length=480, Percent_Identity=37.2916666666667, Blast_Score=290, Evalue=2e-78,
Organism=Homo sapiens, GI28872782, Length=480, Percent_Identity=37.2916666666667, Blast_Score=290, Evalue=3e-78,
Organism=Homo sapiens, GI93277076, Length=450, Percent_Identity=29.7777777777778, Blast_Score=172, Evalue=5e-43,
Organism=Escherichia coli, GI1786882, Length=473, Percent_Identity=82.4524312896406, Blast_Score=832, Evalue=0.0,
Organism=Escherichia coli, GI1787057, Length=433, Percent_Identity=28.8683602771363, Blast_Score=169, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI17553146, Length=464, Percent_Identity=34.051724137931, Blast_Score=258, Evalue=4e-69,
Organism=Caenorhabditis elegans, GI71996771, Length=339, Percent_Identity=27.7286135693215, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI21356207, Length=433, Percent_Identity=37.1824480369515, Blast_Score=285, Evalue=4e-77,
Organism=Drosophila melanogaster, GI19922432, Length=491, Percent_Identity=30.142566191446, Blast_Score=167, Evalue=1e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 53641; Mature: 53510

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQKLHIKTWGCQMNEYDSSKMADLLLSTHGLELTEAPEEADVLLLNTCSIREKAQEKVF
CCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCHHHHHHHHHHH
HQLGRWKELKKNNPNLVIGVGGCVASQEGEHIRHRAPYVDIIFGPQTLHRLPEMINQIRG
HHHHHHHHHHCCCCCEEEECCCEECCCCCCHHHHCCCEEEEEECHHHHHHHHHHHHHHCC
GKSSVVDVSFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKYCTFCVVPYTRGEEVSRPVDD
CCCCEEECCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHEEEEEEECCCCCHHCCCHHH
VLFEIAQLAEQGVREVNLLGQNVNAYRGPTHDGQICSFAELLRLVASIDGIDRLRFTTSH
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHEEECCCC
PIEFTDDIIDVYRDTPELVSFLHLPVQAGSDRVLTMMKRAHTALEYKSIIRKLRAVRPDI
CCCHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
QISSDFIVGFPGETAEDFEQTMNLIAQVNFDMSFSFVYSARPGTPAADMPDDVTEDEKKQ
EECCCEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHH
RLYVLQERINQQAAQFSRRMLGTEQRVLVEGPSKKDIMELTGRTETNRIVNFQGSPEMIG
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHCCCCCCCEEEEECCCHHHHH
KFVDVKITDVYTNSLRGEVVRTEDEMGLRIAQSPQEVMNRTRKEDELGVGRYHG
HHHEEEEEEEECCCCCCCEEECCHHCCCEECCCHHHHHHHHCCHHHCCCCCCCC
>Mature Secondary Structure 
TQKLHIKTWGCQMNEYDSSKMADLLLSTHGLELTEAPEEADVLLLNTCSIREKAQEKVF
CCEEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCHHHHHHHHHHH
HQLGRWKELKKNNPNLVIGVGGCVASQEGEHIRHRAPYVDIIFGPQTLHRLPEMINQIRG
HHHHHHHHHHCCCCCEEEECCCEECCCCCCHHHHCCCEEEEEECHHHHHHHHHHHHHHCC
GKSSVVDVSFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKYCTFCVVPYTRGEEVSRPVDD
CCCCEEECCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHEEEEEEECCCCCHHCCCHHH
VLFEIAQLAEQGVREVNLLGQNVNAYRGPTHDGQICSFAELLRLVASIDGIDRLRFTTSH
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHEEECCCC
PIEFTDDIIDVYRDTPELVSFLHLPVQAGSDRVLTMMKRAHTALEYKSIIRKLRAVRPDI
CCCHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
QISSDFIVGFPGETAEDFEQTMNLIAQVNFDMSFSFVYSARPGTPAADMPDDVTEDEKKQ
EECCCEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHH
RLYVLQERINQQAAQFSRRMLGTEQRVLVEGPSKKDIMELTGRTETNRIVNFQGSPEMIG
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHCCCCCCCEEEEECCCHHHHH
KFVDVKITDVYTNSLRGEVVRTEDEMGLRIAQSPQEVMNRTRKEDELGVGRYHG
HHHEEEEEEEECCCCCCCEEECCHHCCCEECCCHHHHHHHHCCHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA