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The map label for this gene is fdxH [H]

Identifier: 148827168

GI number: 148827168

Start: 461391

End: 463037

Strand: Direct

Name: fdxH [H]

Synonym: CGSHiGG_02500

Alternate gene names: 148827168

Gene position: 461391-463037 (Clockwise)

Preceding gene: 148827167

Following gene: 148827169

Centisome position: 24.45

GC content: 40.13

Gene sequence:

>1647_bases
ATGGCTGGAACTGCTCAAGGCGTTCAAACGCAAGACGTTATTAAAATCTCCGCAACATCTGGTTTAACACCAGCACCACA
AGCGAGAGATCATAAAGTTGAAGTCGCAAAATTAATTGATGTTTCAACTTGTATAGGATGTAAAGCCTGTCAAGTGGGTT
GTTCAGAGTGGAATGATATTCGCTCTGATATCAATGCACAATGTGTTGGGGTTTATGACAATCCAGTTGATCTTGATGCA
AAAGCATGGACGGTAATGCGTTTTAACGAAGTTGAAGAAAACGATCGTTTAGAATGGCTGATTCGTAAAGATGGCTGTAT
GCACTGCACAGAACCTGGTTGTTTAAAGGCTTGTCCTGCACCCGGTGCAATCATTCAATATGCTAACGGTATTGTAGATT
TCCAATCCGATAAATGTATTGGTTGTGGTTATTGTATTGCAGGCTGTCCATTCAATATTCCACGAATGAATCCTGAAGAC
AATCGTGTATACAAATGTACTCTTTGTGTGGATCGTGTTTCTGTGGGACAAGAGCCAGCTTGCGTGAAAACCTGTCCAAC
AGGTGCTATTCGTTTCGGCTCTAAAGAAGAAATGAAAATTTACGCAGAACAACGCGTGGCAGATTTAAAATCCCGTGGTT
ATGAAAACGCAGGACTTTACGATCCTCCAGGTGTAGGCGGTACACACGTAATGTATGTGTTGCATCACGCAGATAAACCT
GAGCTTTACAATGGTCTTCCAAAAGATCCTCAAATTGATTTGAGTATAACCTTATGGAAAGATGTATTGAAACCAGTCGC
TGCAGTTGCAATGGGTGGCCTAGCTCTAGCAGAAGTTGCGCACTACTTAACCGTTGGCCCAAATGTAGAAGAAGATGTGG
AAGATCATCATCACGAATTTGAAGAAAATAAACCATCTAAGGGGAAAATAATGAGTAAAATTGAAATTAGCAACGATACT
CGCGTTATCCGCCATAGAACCCCTGCACGTATCAGTCACTGGATGTTGGTTATTTGCTTTTTTATGACGATGTTCACTGG
TGTTGCATTTTTCTTCCCTGACTTTGCTTGGCTCACAGAAATTTTGGGTACACCACAAATTGCACGCGCCATTCATCCAT
TCACAGGGATTTTAATGTTCTTCGCTTTCATCTACTTAGCGTTATTGTACTGGGATCACAATATTCCAGAAAAAAACGAT
ATTCGCTGGGCAAAAGGTGTTATTGAAGTTCTTAAAGGGAATGAACACGCGGTTGCTGATAATGGCAAATATAACCTTGG
TCAAAAAATGCTCTTTTGGACATTAAACTTGGCAATGGTAACGTTATTAGTCACTGGCATCATTATGTGGCGTCAATATT
TTTCACATTACTTCTCAATCCCAGTATTGCGAATTGCTATTTTACTCCACTCTGCAAGTGCTTTTATGTTATTCACTGGT
ATCTTAGTGCATATATATATGGCATTTTGGGTTAAAGGATCAATTCGCGGTATTGTTGAAGGTTGGGTAACCGTTCGTTG
GGCGAAAAAACACCACCCAAGATGGTATCGTGAAGAAGTTTTATCAAAACTTGAGGAAGATTTACTCAACGAGCAATCTG
GCAAAGTAGGTAAAACCAAAGTCTTATTTAAAGGATTTGGCAAATAA

Upstream 100 bases:

>100_bases
TCTACGAATACCTTAACACCATCTTGGGGAGAGGCAATCACGCAAACACCAGAATACAAAACATTCTTGGTAAATATTGA
AAAAGTTGGGGAGGCATAAT

Downstream 100 bases:

>100_bases
GCTGAAATCCTGAAAAAGAAAAAGTGCGGTTAAAATTGACCGCACTTTTTATTATAAAAGTGAGAGAATATGAGTATCAA
AATCTTATCTGAATCAGAAA

Product: formate dehydrogenase accessory protein

Products: CO2; NADH

Alternate protein names: Formate dehydrogenase subunit beta; FDH subunit beta [H]

Number of amino acids: Translated: 548; Mature: 547

Protein sequence:

>548_residues
MAGTAQGVQTQDVIKISATSGLTPAPQARDHKVEVAKLIDVSTCIGCKACQVGCSEWNDIRSDINAQCVGVYDNPVDLDA
KAWTVMRFNEVEENDRLEWLIRKDGCMHCTEPGCLKACPAPGAIIQYANGIVDFQSDKCIGCGYCIAGCPFNIPRMNPED
NRVYKCTLCVDRVSVGQEPACVKTCPTGAIRFGSKEEMKIYAEQRVADLKSRGYENAGLYDPPGVGGTHVMYVLHHADKP
ELYNGLPKDPQIDLSITLWKDVLKPVAAVAMGGLALAEVAHYLTVGPNVEEDVEDHHHEFEENKPSKGKIMSKIEISNDT
RVIRHRTPARISHWMLVICFFMTMFTGVAFFFPDFAWLTEILGTPQIARAIHPFTGILMFFAFIYLALLYWDHNIPEKND
IRWAKGVIEVLKGNEHAVADNGKYNLGQKMLFWTLNLAMVTLLVTGIIMWRQYFSHYFSIPVLRIAILLHSASAFMLFTG
ILVHIYMAFWVKGSIRGIVEGWVTVRWAKKHHPRWYREEVLSKLEEDLLNEQSGKVGKTKVLFKGFGK

Sequences:

>Translated_548_residues
MAGTAQGVQTQDVIKISATSGLTPAPQARDHKVEVAKLIDVSTCIGCKACQVGCSEWNDIRSDINAQCVGVYDNPVDLDA
KAWTVMRFNEVEENDRLEWLIRKDGCMHCTEPGCLKACPAPGAIIQYANGIVDFQSDKCIGCGYCIAGCPFNIPRMNPED
NRVYKCTLCVDRVSVGQEPACVKTCPTGAIRFGSKEEMKIYAEQRVADLKSRGYENAGLYDPPGVGGTHVMYVLHHADKP
ELYNGLPKDPQIDLSITLWKDVLKPVAAVAMGGLALAEVAHYLTVGPNVEEDVEDHHHEFEENKPSKGKIMSKIEISNDT
RVIRHRTPARISHWMLVICFFMTMFTGVAFFFPDFAWLTEILGTPQIARAIHPFTGILMFFAFIYLALLYWDHNIPEKND
IRWAKGVIEVLKGNEHAVADNGKYNLGQKMLFWTLNLAMVTLLVTGIIMWRQYFSHYFSIPVLRIAILLHSASAFMLFTG
ILVHIYMAFWVKGSIRGIVEGWVTVRWAKKHHPRWYREEVLSKLEEDLLNEQSGKVGKTKVLFKGFGK
>Mature_547_residues
AGTAQGVQTQDVIKISATSGLTPAPQARDHKVEVAKLIDVSTCIGCKACQVGCSEWNDIRSDINAQCVGVYDNPVDLDAK
AWTVMRFNEVEENDRLEWLIRKDGCMHCTEPGCLKACPAPGAIIQYANGIVDFQSDKCIGCGYCIAGCPFNIPRMNPEDN
RVYKCTLCVDRVSVGQEPACVKTCPTGAIRFGSKEEMKIYAEQRVADLKSRGYENAGLYDPPGVGGTHVMYVLHHADKPE
LYNGLPKDPQIDLSITLWKDVLKPVAAVAMGGLALAEVAHYLTVGPNVEEDVEDHHHEFEENKPSKGKIMSKIEISNDTR
VIRHRTPARISHWMLVICFFMTMFTGVAFFFPDFAWLTEILGTPQIARAIHPFTGILMFFAFIYLALLYWDHNIPEKNDI
RWAKGVIEVLKGNEHAVADNGKYNLGQKMLFWTLNLAMVTLLVTGIIMWRQYFSHYFSIPVLRIAILLHSASAFMLFTGI
LVHIYMAFWVKGSIRGIVEGWVTVRWAKKHHPRWYREEVLSKLEEDLLNEQSGKVGKTKVLFKGFGK

Specific function: Allows to use formate as major electron donor during aerobic respiration. The beta chain is an electron transfer unit containing 4 cysteine clusters involved in the formation of iron- sulfur centers. Electrons are transferred from the gamma chain to the m

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cell membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787749, Length=292, Percent_Identity=73.972602739726, Blast_Score=465, Evalue=1e-132,
Organism=Escherichia coli, GI1790326, Length=296, Percent_Identity=71.9594594594595, Blast_Score=465, Evalue=1e-132,
Organism=Escherichia coli, GI1787750, Length=203, Percent_Identity=52.7093596059113, Blast_Score=234, Evalue=1e-62,
Organism=Escherichia coli, GI1790325, Length=197, Percent_Identity=51.2690355329949, Blast_Score=227, Evalue=1e-60,
Organism=Escherichia coli, GI1789370, Length=183, Percent_Identity=32.2404371584699, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1787872, Length=162, Percent_Identity=33.3333333333333, Blast_Score=100, Evalue=4e-22,
Organism=Escherichia coli, GI1787122, Length=162, Percent_Identity=32.7160493827161, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI2367345, Length=183, Percent_Identity=30.0546448087432, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI226510944, Length=215, Percent_Identity=27.906976744186, Blast_Score=87, Evalue=4e-18,
Organism=Escherichia coli, GI87082179, Length=173, Percent_Identity=28.9017341040462, Blast_Score=82, Evalue=8e-17,
Organism=Escherichia coli, GI1787740, Length=100, Percent_Identity=36, Blast_Score=69, Evalue=9e-13,
Organism=Escherichia coli, GI1787478, Length=100, Percent_Identity=36, Blast_Score=69, Evalue=9e-13,
Organism=Escherichia coli, GI87082180, Length=185, Percent_Identity=28.6486486486486, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI1787963, Length=186, Percent_Identity=24.1935483870968, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006470
- InterPro:   IPR014603
- InterPro:   IPR015246 [H]

Pfam domain/function: PF09163 Form-deh_trans [H]

EC number: 1.2.1.2

Molecular weight: Translated: 61550; Mature: 61419

Theoretical pI: Translated: 7.23; Mature: 7.23

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGTAQGVQTQDVIKISATSGLTPAPQARDHKVEVAKLIDVSTCIGCKACQVGCSEWNDI
CCCCCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHCCHHHHHHH
RSDINAQCVGVYDNPVDLDAKAWTVMRFNEVEENDRLEWLIRKDGCMHCTEPGCLKACPA
HHCCCCEEEEEECCCCCCCCCEEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCCC
PGAIIQYANGIVDFQSDKCIGCGYCIAGCPFNIPRMNPEDNRVYKCTLCVDRVSVGQEPA
CCHHHHHHCCCEEECCCCEEECCHHHCCCCCCCCCCCCCCCCEEEEEEEEEHHHCCCCCC
CVKTCPTGAIRFGSKEEMKIYAEQRVADLKSRGYENAGLYDPPGVGGTHVMYVLHHADKP
CEEECCCCCEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCC
ELYNGLPKDPQIDLSITLWKDVLKPVAAVAMGGLALAEVAHYLTVGPNVEEDVEDHHHEF
HHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
EENKPSKGKIMSKIEISNDTRVIRHRTPARISHWMLVICFFMTMFTGVAFFFPDFAWLTE
HCCCCCCCCEEEEEEECCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
ILGTPQIARAIHPFTGILMFFAFIYLALLYWDHNIPEKNDIRWAKGVIEVLKGNEHAVAD
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCEECC
NGKYNLGQKMLFWTLNLAMVTLLVTGIIMWRQYFSHYFSIPVLRIAILLHSASAFMLFTG
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
ILVHIYMAFWVKGSIRGIVEGWVTVRWAKKHHPRWYREEVLSKLEEDLLNEQSGKVGKTK
HHHHHHHHHHHHHHHHHHHHHHEEEEEHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCEE
VLFKGFGK
EEECCCCC
>Mature Secondary Structure 
AGTAQGVQTQDVIKISATSGLTPAPQARDHKVEVAKLIDVSTCIGCKACQVGCSEWNDI
CCCCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHCCHHHHHHH
RSDINAQCVGVYDNPVDLDAKAWTVMRFNEVEENDRLEWLIRKDGCMHCTEPGCLKACPA
HHCCCCEEEEEECCCCCCCCCEEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCCC
PGAIIQYANGIVDFQSDKCIGCGYCIAGCPFNIPRMNPEDNRVYKCTLCVDRVSVGQEPA
CCHHHHHHCCCEEECCCCEEECCHHHCCCCCCCCCCCCCCCCEEEEEEEEEHHHCCCCCC
CVKTCPTGAIRFGSKEEMKIYAEQRVADLKSRGYENAGLYDPPGVGGTHVMYVLHHADKP
CEEECCCCCEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCC
ELYNGLPKDPQIDLSITLWKDVLKPVAAVAMGGLALAEVAHYLTVGPNVEEDVEDHHHEF
HHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
EENKPSKGKIMSKIEISNDTRVIRHRTPARISHWMLVICFFMTMFTGVAFFFPDFAWLTE
HCCCCCCCCEEEEEEECCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
ILGTPQIARAIHPFTGILMFFAFIYLALLYWDHNIPEKNDIRWAKGVIEVLKGNEHAVAD
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCEECC
NGKYNLGQKMLFWTLNLAMVTLLVTGIIMWRQYFSHYFSIPVLRIAILLHSASAFMLFTG
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
ILVHIYMAFWVKGSIRGIVEGWVTVRWAKKHHPRWYREEVLSKLEEDLLNEQSGKVGKTK
HHHHHHHHHHHHHHHHHHHHHHEEEEEHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCEE
VLFKGFGK
EEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]