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The map label for this gene is ptsI [H]

Identifier: 148827135

GI number: 148827135

Start: 412953

End: 414680

Strand: Reverse

Name: ptsI [H]

Synonym: CGSHiGG_02310

Alternate gene names: 148827135

Gene position: 414680-412953 (Counterclockwise)

Preceding gene: 148827136

Following gene: 148827134

Centisome position: 21.97

GC content: 37.56

Gene sequence:

>1728_bases
ATGATTTCTGGCATTCTCGCATCTCCAGGTATTGCCTTTGGTAAGGCACTTGTTCTTAAAGAAGAAAAGATTGTTCTTGA
TACACAAAAAATTACTGATGATCAAATTGACGCAGAAGTCGCTCGATTTTATGAAGGGCGTGCTGCGGCGGTAGAACAAC
TTAATTCAATTAGAGAACGTGCATTAATTTCTTTAGGAGAAGAAAAAGCGGCAATCTTTGAAGGTCACTTGATGATTCTT
GAGGATGAAGAATTAGAAGAAGAAATTCTTGATTATCTTCGTTCAAATAAAGTGAATGCAGGCGTTGCGGCGAGTAAAAT
TTTAGATCAACAAGCGACAATGTTATCTGAAATTGATGATGAATATTTGAAAGAACGTGCAGGCGATATTCGGGATATTG
CTAACCGTTTAGTTAAAAATATTTTGGGAATGTACATTGTTGATCTTGGTGATATTCAGGAAGAATCTATTCTTGTTGCT
TATGATCTGACTCCCTCAGAAACAGCTCAACTTAATCTTGAAAAAGTATTAGGGGTTGTCACTGATATTGGTGGTAGAAC
CTCACATACTTCTATTATGGCGCGTTCTTTAGAATTGCCAGCGATTGTCGGCACAAATAAAGTGACTAAATTAGTCAATA
CGGGCGATTATTTAATTTTAGATGCTGTCAATAACCAAGTTTATATTAATCCAACAGCATCTCAGATTGATGAATTAAAA
GCACTTGAAGCGAAAATTTCTGAGGAAAAAGCAGAGTTAGCTAAATTAAAAGATCTTCCTGCAATCACATTAGATGGACA
TAAGGTTGATGTGGTTGCGAATATCGGGACGATTCGTGATTGTGACGGCGCAGAACGTAATGGAGCGGAAGGGATTGGGT
TATACCGAACTGAATTCTTATTTATGGATCGTGAACAGCTTCCCACAGAAGAAGAACAATTTATTGCCTATAAACAAGTG
GTAGAAGCAATGAACGGACGTTTAACAGTGATTCGTACTATGGATATTGGTGGCGATAAAGAGCTTTCATATTTAGATTT
ACCAAAAGAAATGAATCCATTCTTGGGATGGCGTGCGATTCGCATTGCATTAGATCGTCGTGAAATTTTAAATGCACAAT
TACGTGCGGTATTGCGTGCTTCAGCTTTTGGTAAATTGGCAGTGATGTTCCCGATGATTATTTCGGTAGAAGAAATTCGA
GAATTAAAAGCGGTTATTGAAACATTAAAGGCGGAATTACGTGAAGAAGGGAGAGTTTTTGATAATAACATACAAGTGGG
TGTCATGGTTGAAACTCCCTCTGCGGCGGTAAATGCAAAATTTTTAGCAAAAGAAGTAGATTTCTTTAGTATCGGAACTA
ATGATTTAACCCAATATACCTTGGCGGTGGATCGCGGTAATGAGTTTATTTCCCATCTTTATAATCCGATGCATCCCTCT
GTGCTTGGTTTAATTAAACAAGTGATTGATGCCTCTCACGCAGAAGGAAAATGGACAGGAATGTGTGGGGAATTAGCTGG
GGATGAACGTGCAACTTTATTGTTACTTGGTATGGGATTAGATGAATTTAGCATGAGTGCGATTTCTGTACCTCGCATTA
AAAAATTAATCCGTAATGTAAATTTCCAAGATGCAAAAGCGCTTGCTGATGCCGCATTACAAAAACCGACCGCTGCAGAA
ATTGATCAATTAATTGAAGAATTTTTGCTGGAAAATTCATTAAATTAA

Upstream 100 bases:

>100_bases
TTAATTCCAACTTTAGAATAATTCTTACTTAGCCATAAAATTGTGAAATTTTATGGCTATTGTTTATTTATTACTTTGTT
AATTGTTTCGGAAGGTATCT

Downstream 100 bases:

>100_bases
ACAAAAGTATTTGATTTTACGTTAAAATAGCAGCCACCTTGAATGGATAGGAGATTAAAATGGGCTTATTTGACAAGTTA
TTTGGTTCAAAAGAAAACAA

Product: phosphoenolpyruvate-protein phosphotransferase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 575; Mature: 575

Protein sequence:

>575_residues
MISGILASPGIAFGKALVLKEEKIVLDTQKITDDQIDAEVARFYEGRAAAVEQLNSIRERALISLGEEKAAIFEGHLMIL
EDEELEEEILDYLRSNKVNAGVAASKILDQQATMLSEIDDEYLKERAGDIRDIANRLVKNILGMYIVDLGDIQEESILVA
YDLTPSETAQLNLEKVLGVVTDIGGRTSHTSIMARSLELPAIVGTNKVTKLVNTGDYLILDAVNNQVYINPTASQIDELK
ALEAKISEEKAELAKLKDLPAITLDGHKVDVVANIGTIRDCDGAERNGAEGIGLYRTEFLFMDREQLPTEEEQFIAYKQV
VEAMNGRLTVIRTMDIGGDKELSYLDLPKEMNPFLGWRAIRIALDRREILNAQLRAVLRASAFGKLAVMFPMIISVEEIR
ELKAVIETLKAELREEGRVFDNNIQVGVMVETPSAAVNAKFLAKEVDFFSIGTNDLTQYTLAVDRGNEFISHLYNPMHPS
VLGLIKQVIDASHAEGKWTGMCGELAGDERATLLLLGMGLDEFSMSAISVPRIKKLIRNVNFQDAKALADAALQKPTAAE
IDQLIEEFLLENSLN

Sequences:

>Translated_575_residues
MISGILASPGIAFGKALVLKEEKIVLDTQKITDDQIDAEVARFYEGRAAAVEQLNSIRERALISLGEEKAAIFEGHLMIL
EDEELEEEILDYLRSNKVNAGVAASKILDQQATMLSEIDDEYLKERAGDIRDIANRLVKNILGMYIVDLGDIQEESILVA
YDLTPSETAQLNLEKVLGVVTDIGGRTSHTSIMARSLELPAIVGTNKVTKLVNTGDYLILDAVNNQVYINPTASQIDELK
ALEAKISEEKAELAKLKDLPAITLDGHKVDVVANIGTIRDCDGAERNGAEGIGLYRTEFLFMDREQLPTEEEQFIAYKQV
VEAMNGRLTVIRTMDIGGDKELSYLDLPKEMNPFLGWRAIRIALDRREILNAQLRAVLRASAFGKLAVMFPMIISVEEIR
ELKAVIETLKAELREEGRVFDNNIQVGVMVETPSAAVNAKFLAKEVDFFSIGTNDLTQYTLAVDRGNEFISHLYNPMHPS
VLGLIKQVIDASHAEGKWTGMCGELAGDERATLLLLGMGLDEFSMSAISVPRIKKLIRNVNFQDAKALADAALQKPTAAE
IDQLIEEFLLENSLN
>Mature_575_residues
MISGILASPGIAFGKALVLKEEKIVLDTQKITDDQIDAEVARFYEGRAAAVEQLNSIRERALISLGEEKAAIFEGHLMIL
EDEELEEEILDYLRSNKVNAGVAASKILDQQATMLSEIDDEYLKERAGDIRDIANRLVKNILGMYIVDLGDIQEESILVA
YDLTPSETAQLNLEKVLGVVTDIGGRTSHTSIMARSLELPAIVGTNKVTKLVNTGDYLILDAVNNQVYINPTASQIDELK
ALEAKISEEKAELAKLKDLPAITLDGHKVDVVANIGTIRDCDGAERNGAEGIGLYRTEFLFMDREQLPTEEEQFIAYKQV
VEAMNGRLTVIRTMDIGGDKELSYLDLPKEMNPFLGWRAIRIALDRREILNAQLRAVLRASAFGKLAVMFPMIISVEEIR
ELKAVIETLKAELREEGRVFDNNIQVGVMVETPSAAVNAKFLAKEVDFFSIGTNDLTQYTLAVDRGNEFISHLYNPMHPS
VLGLIKQVIDASHAEGKWTGMCGELAGDERATLLLLGMGLDEFSMSAISVPRIKKLIRNVNFQDAKALADAALQKPTAAE
IDQLIEEFLLENSLN

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=574, Percent_Identity=70.7317073170732, Blast_Score=825, Evalue=0.0,
Organism=Escherichia coli, GI1788726, Length=577, Percent_Identity=37.6083188908146, Blast_Score=355, Evalue=6e-99,
Organism=Escherichia coli, GI48994992, Length=521, Percent_Identity=37.236084452975, Blast_Score=326, Evalue=2e-90,
Organism=Escherichia coli, GI1789193, Length=579, Percent_Identity=32.8151986183074, Blast_Score=291, Evalue=7e-80,
Organism=Escherichia coli, GI1787994, Length=430, Percent_Identity=28.3720930232558, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI226510935, Length=152, Percent_Identity=28.9473684210526, Blast_Score=66, Evalue=5e-12,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 63535; Mature: 63535

Theoretical pI: Translated: 4.41; Mature: 4.41

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISGILASPGIAFGKALVLKEEKIVLDTQKITDDQIDAEVARFYEGRAAAVEQLNSIRER
CCCCCCCCCCHHHCCEEEEECCEEEEEHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHH
ALISLGEEKAAIFEGHLMILEDEELEEEILDYLRSNKVNAGVAASKILDQQATMLSEIDD
HHHHCCCCHHEEEECCEEEEECHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
EYLKERAGDIRDIANRLVKNILGMYIVDLGDIQEESILVAYDLTPSETAQLNLEKVLGVV
HHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHH
TDIGGRTSHTSIMARSLELPAIVGTNKVTKLVNTGDYLILDAVNNQVYINPTASQIDELK
HHCCCCCCHHHHHHHHCCCCEEECCCHHHHHCCCCCEEEEEECCCEEEECCCHHHHHHHH
ALEAKISEEKAELAKLKDLPAITLDGHKVDVVANIGTIRDCDGAERNGAEGIGLYRTEFL
HHHHHHHHHHHHHHHHHCCCEEEECCCEEEEEEECCCCCCCCCCCCCCCCCCCEEEHEEE
FMDREQLPTEEEQFIAYKQVVEAMNGRLTVIRTMDIGGDKELSYLDLPKEMNPFLGWRAI
EECHHHCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCEEECCCHHCCHHHCCEEE
RIALDRREILNAQLRAVLRASAFGKLAVMFPMIISVEEIRELKAVIETLKAELREEGRVF
HEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
DNNIQVGVMVETPSAAVNAKFLAKEVDFFSIGTNDLTQYTLAVDRGNEFISHLYNPMHPS
CCCEEEEEEEECCCCHHHHHHHHHHCCEEECCCCCCEEEEEEEECCHHHHHHHCCCCCHH
VLGLIKQVIDASHAEGKWTGMCGELAGDERATLLLLGMGLDEFSMSAISVPRIKKLIRNV
HHHHHHHHHCCCCCCCCCCCCCHHCCCCCCEEEEEEECCCCHHHHHHCCHHHHHHHHHCC
NFQDAKALADAALQKPTAAEIDQLIEEFLLENSLN
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MISGILASPGIAFGKALVLKEEKIVLDTQKITDDQIDAEVARFYEGRAAAVEQLNSIRER
CCCCCCCCCCHHHCCEEEEECCEEEEEHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHH
ALISLGEEKAAIFEGHLMILEDEELEEEILDYLRSNKVNAGVAASKILDQQATMLSEIDD
HHHHCCCCHHEEEECCEEEEECHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
EYLKERAGDIRDIANRLVKNILGMYIVDLGDIQEESILVAYDLTPSETAQLNLEKVLGVV
HHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHH
TDIGGRTSHTSIMARSLELPAIVGTNKVTKLVNTGDYLILDAVNNQVYINPTASQIDELK
HHCCCCCCHHHHHHHHCCCCEEECCCHHHHHCCCCCEEEEEECCCEEEECCCHHHHHHHH
ALEAKISEEKAELAKLKDLPAITLDGHKVDVVANIGTIRDCDGAERNGAEGIGLYRTEFL
HHHHHHHHHHHHHHHHHCCCEEEECCCEEEEEEECCCCCCCCCCCCCCCCCCCEEEHEEE
FMDREQLPTEEEQFIAYKQVVEAMNGRLTVIRTMDIGGDKELSYLDLPKEMNPFLGWRAI
EECHHHCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCEEECCCHHCCHHHCCEEE
RIALDRREILNAQLRAVLRASAFGKLAVMFPMIISVEEIRELKAVIETLKAELREEGRVF
HEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
DNNIQVGVMVETPSAAVNAKFLAKEVDFFSIGTNDLTQYTLAVDRGNEFISHLYNPMHPS
CCCEEEEEEEECCCCHHHHHHHHHHCCEEECCCCCCEEEEEEEECCHHHHHHHCCCCCHH
VLGLIKQVIDASHAEGKWTGMCGELAGDERATLLLLGMGLDEFSMSAISVPRIKKLIRNV
HHHHHHHHHCCCCCCCCCCCCCHHCCCCCCEEEEEEECCCCHHHHHHCCHHHHHHHHHCC
NFQDAKALADAALQKPTAAEIDQLIEEFLLENSLN
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]