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The map label for this gene is metE [H]

Identifier: 148827125

GI number: 148827125

Start: 401039

End: 403309

Strand: Reverse

Name: metE [H]

Synonym: CGSHiGG_02260

Alternate gene names: 148827125

Gene position: 403309-401039 (Counterclockwise)

Preceding gene: 148827126

Following gene: 148827123

Centisome position: 21.37

GC content: 39.76

Gene sequence:

>2271_bases
ATGACAACATCACATATTTTAGGCTTTCCTCGTGTAGGGGCAAAACGTGAATTAAAATTCGCACAAGAACGTTATTGGCG
TAAAGAATTAGCAGAGCAAGATTTATTAGATTTAGCGAAAGCGTTGCGTGAAAAAAACTGGAAACATCAAGCGGCTGCGA
ATGCGGATTTCGTTGCAGTGGGCGATTTCACGTTCTACGATCATATTTTAGATTTACAAGTGGCAACAGGGGCAATTCCT
GCTCGTTTTGGTTTTGATAGCCAAAATTTAACCCTTGATCAATATTTCCAACTTGCACGTGGTAACAAAGATCAATTCGC
AATTGAAATGACCAAATGGTTTGATACGAACTATCACTATCTTGTGCCTGAGTTCCAAAAATCGACCGCTTTCAAAGCTA
ATCCAGCACATTATGTAAACCAAATCCGTGAAGCAAAAGCCTTAGGCTTAAACTTCAAACCTGTGATTGTTGGCCCATTA
ACATTCTTATGGTTAGGTAAAGAAAAAGGCGAAGCATTTAACCGTTTCGATTTATTAAATCAATTAGTGCCTGTTTATGT
TGAAATATTAAATGCATTAGTGGCTGAAGGGGCAGAATGGATTCAAATTGATGAACCTGCATTAGCATTAGATTTACCAG
CAGAATGGGTTGAAGCCTATAAATCTGTTTACGCTGAATTAAGCAAAGTGAACGCAAAATTATTATTAGCCACTTATTTT
GGTTCAGTTGCAGAACACGCTGAATTATTAAAAGCCTTACCTGTTGCAGGCTTGCATTTAGATTTAGTTCGTGCACCAGA
ACAACTTGCGGCATTTGAAGATTACAGCAAAGTGTTATCAGCTGGTGTGATTGAAGGTCGTAATATCTGGCGTGCAAACT
TAAACAAAGTGTTAGATGTATTAGAGCCATTAAAAGCAAAATTAGGTGAGCGTTTATGGATTGCACCAAGCTGTTCATTA
TTGCATACCCCATTTGATTTAGAAGTGGAAGTACAATTAAAAGAAAAAAATACCGCACTTTACAGCTGGTTATCTTTCAC
GCTACAAAAAGTAGAAGAATTAAACGTATTAAAACAAGCGTTAAATAATGGCAGAGCGTCTGTACAAGCAGCATTAGATG
CAAGCCAAGCGGCAGCAGATGCACGCGCAACCTCAAAAGAAATTCATCGTCCTGAAGTGGCAGAACGATTAGCAAACTTG
CCAAAAGGTGCGGATCAACGTAAATCGCCATTTGCAGAACGTATCGTTAAGCAAAATGCGTGGTTAAATTTACCGCTTCT
GCCAACTACAAACATTGGTTCATTCCCACAAACTACTGAAATTCGTCACGCCCGTGCAAGTTTCAAAAAAGGCGAGTTAT
CTCTAGCAGATTACGAAGCGGCAATGAAAAAAGAGATCGAATATGTAGTACGTCGCCAAGAAGAATTAGATTTAGATGTG
TTAGTTCACGGTGAAGCAGAACGTAACGACATGGTGGAATACTTTGGGGAATTATTAGATGGTTTCGCATTCACTAAATT
TGGTTGGGTACAAAGCTACGGTTCACGTTGTGTAAAACCACCAGTAATTTACGGTGATGTTATCCGCCCTGAGCCAATGA
CAGTACGCTGGTCACAATATGCGCAAAGTTTAACTAACCGTGTAATGAAAGGAATGCTCACAGGCCCTGTGACTATTTTA
CAATGGTCATTCGTGCGTAACGATATTCCACGTTCAACCGTATGTAAACAAATCGGCGTAGCATTATCTGATGAAGTGTT
AGATTTAGAAGCAGCAGGCATTAAAGTCATTCAAATTGATGAGCCTGCAATTCGTGAAGGTTTACCACTTAAACGTGCAG
ATTGGGATGCATACTTACAATGGGCAGGCGAAGCATTCCGTTTAAGCTCAATGGGCGTACAAGATGATACGCAAATTCAC
ACCCATATGTGTTACTCTGAATTTAACGACATCTTACCTGCCATTGCGGCATTAGATGCGGATGTGATTACTATCGAAAC
TTCACGTTCAGATATGGAATTATTAACCGCATTTGCAGATTTCAAATATCCAAATGATATTGGCCCAGGTGTTTATGATA
TTCACAGCCCTCGTGTGCCAACGGCAACGGAAGTAGAACATTTATTACGCAAAGCATTAAATGTCATTCCAAAAGAACGC
TTATGGGTAAACCCAGACTGCGGTTTAAAAACGCGTGGTTGGACAGAAACCATCGACCAATTAAAAGTGATGGTGGATGT
AACCAAAAAATTACGTGCGGAATTAGCGTAA

Upstream 100 bases:

>100_bases
TAATAAGTTTGCATAATAAATGAAGATCAAGCATTCTATATTGGTATAGACGTTTAGCCGTCTAAATGATTAAAAAAATT
ACTTTAAATTGGGAGTTATT

Downstream 100 bases:

>100_bases
GTCATAATACATAAAGAATAATTTTCAAACACAACATAATTAAAGCCGAATCTCTATAGGATTCGGTTTTTCTTATAGAT
AAAATGTTGAAATATAATAA

Product: 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase

Products: NA

Alternate protein names: Cobalamin-independent methionine synthase; Methionine synthase, vitamin-B12 independent isozyme [H]

Number of amino acids: Translated: 756; Mature: 755

Protein sequence:

>756_residues
MTTSHILGFPRVGAKRELKFAQERYWRKELAEQDLLDLAKALREKNWKHQAAANADFVAVGDFTFYDHILDLQVATGAIP
ARFGFDSQNLTLDQYFQLARGNKDQFAIEMTKWFDTNYHYLVPEFQKSTAFKANPAHYVNQIREAKALGLNFKPVIVGPL
TFLWLGKEKGEAFNRFDLLNQLVPVYVEILNALVAEGAEWIQIDEPALALDLPAEWVEAYKSVYAELSKVNAKLLLATYF
GSVAEHAELLKALPVAGLHLDLVRAPEQLAAFEDYSKVLSAGVIEGRNIWRANLNKVLDVLEPLKAKLGERLWIAPSCSL
LHTPFDLEVEVQLKEKNTALYSWLSFTLQKVEELNVLKQALNNGRASVQAALDASQAAADARATSKEIHRPEVAERLANL
PKGADQRKSPFAERIVKQNAWLNLPLLPTTNIGSFPQTTEIRHARASFKKGELSLADYEAAMKKEIEYVVRRQEELDLDV
LVHGEAERNDMVEYFGELLDGFAFTKFGWVQSYGSRCVKPPVIYGDVIRPEPMTVRWSQYAQSLTNRVMKGMLTGPVTIL
QWSFVRNDIPRSTVCKQIGVALSDEVLDLEAAGIKVIQIDEPAIREGLPLKRADWDAYLQWAGEAFRLSSMGVQDDTQIH
THMCYSEFNDILPAIAALDADVITIETSRSDMELLTAFADFKYPNDIGPGVYDIHSPRVPTATEVEHLLRKALNVIPKER
LWVNPDCGLKTRGWTETIDQLKVMVDVTKKLRAELA

Sequences:

>Translated_756_residues
MTTSHILGFPRVGAKRELKFAQERYWRKELAEQDLLDLAKALREKNWKHQAAANADFVAVGDFTFYDHILDLQVATGAIP
ARFGFDSQNLTLDQYFQLARGNKDQFAIEMTKWFDTNYHYLVPEFQKSTAFKANPAHYVNQIREAKALGLNFKPVIVGPL
TFLWLGKEKGEAFNRFDLLNQLVPVYVEILNALVAEGAEWIQIDEPALALDLPAEWVEAYKSVYAELSKVNAKLLLATYF
GSVAEHAELLKALPVAGLHLDLVRAPEQLAAFEDYSKVLSAGVIEGRNIWRANLNKVLDVLEPLKAKLGERLWIAPSCSL
LHTPFDLEVEVQLKEKNTALYSWLSFTLQKVEELNVLKQALNNGRASVQAALDASQAAADARATSKEIHRPEVAERLANL
PKGADQRKSPFAERIVKQNAWLNLPLLPTTNIGSFPQTTEIRHARASFKKGELSLADYEAAMKKEIEYVVRRQEELDLDV
LVHGEAERNDMVEYFGELLDGFAFTKFGWVQSYGSRCVKPPVIYGDVIRPEPMTVRWSQYAQSLTNRVMKGMLTGPVTIL
QWSFVRNDIPRSTVCKQIGVALSDEVLDLEAAGIKVIQIDEPAIREGLPLKRADWDAYLQWAGEAFRLSSMGVQDDTQIH
THMCYSEFNDILPAIAALDADVITIETSRSDMELLTAFADFKYPNDIGPGVYDIHSPRVPTATEVEHLLRKALNVIPKER
LWVNPDCGLKTRGWTETIDQLKVMVDVTKKLRAELA
>Mature_755_residues
TTSHILGFPRVGAKRELKFAQERYWRKELAEQDLLDLAKALREKNWKHQAAANADFVAVGDFTFYDHILDLQVATGAIPA
RFGFDSQNLTLDQYFQLARGNKDQFAIEMTKWFDTNYHYLVPEFQKSTAFKANPAHYVNQIREAKALGLNFKPVIVGPLT
FLWLGKEKGEAFNRFDLLNQLVPVYVEILNALVAEGAEWIQIDEPALALDLPAEWVEAYKSVYAELSKVNAKLLLATYFG
SVAEHAELLKALPVAGLHLDLVRAPEQLAAFEDYSKVLSAGVIEGRNIWRANLNKVLDVLEPLKAKLGERLWIAPSCSLL
HTPFDLEVEVQLKEKNTALYSWLSFTLQKVEELNVLKQALNNGRASVQAALDASQAAADARATSKEIHRPEVAERLANLP
KGADQRKSPFAERIVKQNAWLNLPLLPTTNIGSFPQTTEIRHARASFKKGELSLADYEAAMKKEIEYVVRRQEELDLDVL
VHGEAERNDMVEYFGELLDGFAFTKFGWVQSYGSRCVKPPVIYGDVIRPEPMTVRWSQYAQSLTNRVMKGMLTGPVTILQ
WSFVRNDIPRSTVCKQIGVALSDEVLDLEAAGIKVIQIDEPAIREGLPLKRADWDAYLQWAGEAFRLSSMGVQDDTQIHT
HMCYSEFNDILPAIAALDADVITIETSRSDMELLTAFADFKYPNDIGPGVYDIHSPRVPTATEVEHLLRKALNVIPKERL
WVNPDCGLKTRGWTETIDQLKVMVDVTKKLRAELA

Specific function: Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation [H]

COG id: COG0620

COG function: function code E; Methionine synthase II (cobalamin-independent)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vitamin-B12 independent methionine synthase family [H]

Homologues:

Organism=Escherichia coli, GI2367304, Length=757, Percent_Identity=54.557463672391, Blast_Score=821, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6320936, Length=775, Percent_Identity=46.3225806451613, Blast_Score=673, Evalue=0.0,

Paralogues:

None

Copy number: 45,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013215
- InterPro:   IPR006276
- InterPro:   IPR002629 [H]

Pfam domain/function: PF08267 Meth_synt_1; PF01717 Meth_synt_2 [H]

EC number: =2.1.1.14 [H]

Molecular weight: Translated: 85193; Mature: 85062

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTSHILGFPRVGAKRELKFAQERYWRKELAEQDLLDLAKALREKNWKHQAAANADFVAV
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCEEEE
GDFTFYDHILDLQVATGAIPARFGFDSQNLTLDQYFQLARGNKDQFAIEMTKWFDTNYHY
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEEEHHHCCCCEE
LVPEFQKSTAFKANPAHYVNQIREAKALGLNFKPVIVGPLTFLWLGKEKGEAFNRFDLLN
ECCCHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEECCEEEEEECCCCCCHHHHHHHHH
QLVPVYVEILNALVAEGAEWIQIDEPALALDLPAEWVEAYKSVYAELSKVNAKLLLATYF
HHHHHHHHHHHHHHHCCCCEEEECCCCEEEECCHHHHHHHHHHHHHHHHCCHHHHHHHHH
GSVAEHAELLKALPVAGLHLDLVRAPEQLAAFEDYSKVLSAGVIEGRNIWRANLNKVLDV
HHHHHHHHHHHHCCCCCCCHHHHHCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
LEPLKAKLGERLWIAPSCSLLHTPFDLEVEVQLKEKNTALYSWLSFTLQKVEELNVLKQA
HHHHHHHHCCEEEECCCCCEEECCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
LNNGRASVQAALDASQAAADARATSKEIHRPEVAERLANLPKGADQRKSPFAERIVKQNA
HCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHCCHHHHHHHHCCC
WLNLPLLPTTNIGSFPQTTEIRHARASFKKGELSLADYEAAMKKEIEYVVRRQEELDLDV
CEECCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEE
LVHGEAERNDMVEYFGELLDGFAFTKFGWVQSYGSRCVKPPVIYGDVIRPEPMTVRWSQY
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCEEEHHHH
AQSLTNRVMKGMLTGPVTILQWSFVRNDIPRSTVCKQIGVALSDEVLDLEAAGIKVIQID
HHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHCCHHCCHHHEEECCCEEEEEEC
EPAIREGLPLKRADWDAYLQWAGEAFRLSSMGVQDDTQIHTHMCYSEFNDILPAIAALDA
CHHHHCCCCCCCCCHHHHHHHCCCHHEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
DVITIETSRSDMELLTAFADFKYPNDIGPGVYDIHSPRVPTATEVEHLLRKALNVIPKER
CEEEEECCCHHHHHHHHHHHCCCCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHCCCCC
LWVNPDCGLKTRGWTETIDQLKVMVDVTKKLRAELA
EEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TTSHILGFPRVGAKRELKFAQERYWRKELAEQDLLDLAKALREKNWKHQAAANADFVAV
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCEEEE
GDFTFYDHILDLQVATGAIPARFGFDSQNLTLDQYFQLARGNKDQFAIEMTKWFDTNYHY
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEEEHHHCCCCEE
LVPEFQKSTAFKANPAHYVNQIREAKALGLNFKPVIVGPLTFLWLGKEKGEAFNRFDLLN
ECCCHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEECCEEEEEECCCCCCHHHHHHHHH
QLVPVYVEILNALVAEGAEWIQIDEPALALDLPAEWVEAYKSVYAELSKVNAKLLLATYF
HHHHHHHHHHHHHHHCCCCEEEECCCCEEEECCHHHHHHHHHHHHHHHHCCHHHHHHHHH
GSVAEHAELLKALPVAGLHLDLVRAPEQLAAFEDYSKVLSAGVIEGRNIWRANLNKVLDV
HHHHHHHHHHHHCCCCCCCHHHHHCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
LEPLKAKLGERLWIAPSCSLLHTPFDLEVEVQLKEKNTALYSWLSFTLQKVEELNVLKQA
HHHHHHHHCCEEEECCCCCEEECCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
LNNGRASVQAALDASQAAADARATSKEIHRPEVAERLANLPKGADQRKSPFAERIVKQNA
HCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHCCHHHHHHHHCCC
WLNLPLLPTTNIGSFPQTTEIRHARASFKKGELSLADYEAAMKKEIEYVVRRQEELDLDV
CEECCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEE
LVHGEAERNDMVEYFGELLDGFAFTKFGWVQSYGSRCVKPPVIYGDVIRPEPMTVRWSQY
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCEEEHHHH
AQSLTNRVMKGMLTGPVTILQWSFVRNDIPRSTVCKQIGVALSDEVLDLEAAGIKVIQID
HHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHCCHHCCHHHEEECCCEEEEEEC
EPAIREGLPLKRADWDAYLQWAGEAFRLSSMGVQDDTQIHTHMCYSEFNDILPAIAALDA
CHHHHCCCCCCCCCHHHHHHHCCCHHEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
DVITIETSRSDMELLTAFADFKYPNDIGPGVYDIHSPRVPTATEVEHLLRKALNVIPKER
CEEEEECCCHHHHHHHHHHHCCCCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHCCCCC
LWVNPDCGLKTRGWTETIDQLKVMVDVTKKLRAELA
EEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA