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The map label for this gene is rfaB [C]

Identifier: 148827120

GI number: 148827120

Start: 396762

End: 397652

Strand: Reverse

Name: rfaB [C]

Synonym: CGSHiGG_02235

Alternate gene names: 148827120

Gene position: 397652-396762 (Counterclockwise)

Preceding gene: 148827121

Following gene: 148827119

Centisome position: 21.07

GC content: 29.29

Gene sequence:

>891_bases
ATGAATAAATTGCCCTATTCATTGAAAAAAGATTATTTTTCTATCACTAAAAAATTAAGAGAATTAGTTAAAGAATTACA
GCTGGATACCTTAATTGTGGTTGATGGTGCAATTATGCTTTTTTCTGCTTTGGCGTTGGTTAATTTAAATATAAAACATA
TTTTATGGGAGCATTATTCTTTTAATTTTACGGGCAATCGTCTCGTTCGCACGCTCGGTAAATATTTAGCTGTAACAACT
TGCGATAAAATAGTTACATTGACAGAATCAGAAAAAACGCTGTGGCAAGAAAAATTTAAAACAAATAATATAATTAGTAT
TGCTAATCCAAATACGCTTCTTCCAAAAAATAAATTAGCAAAATTGGAGAATAAAACTATTCTATCTGTAGGGCATTTAT
TTTCTTATAAAGGTTTTGATTATTTATTAAAAGTTTGGCAAGTTTTGGAAAAAAAATATCCAAATTGGAATTTAAAAATA
GTAGGATCGGGTGAGGAAGAAGAGAATTTAAAAAATCTTGCTAAAGCATTGGATATTGAAGATTCAGTTAATTTTATTCC
TCGTACTAATGATGTCTCTTTTTATTATGAAAGCAGTTCAATTTATTGTTTACCTTCACAAACAGAGGGCTTGCCTCTAG
TGCTTATTGAGGCGATGGCATTTGGCTTACCTATTGTTGCCTTTAATTGTTCTCCTGGCGTAAAGCAATTAGTCGAACAT
AAAGAAAATGGTTTTCTTTGTGAACAAAATAATATTGAAGAAATGGTTAAAGGTTTAGATTTATTAATCAATAATCCTGA
ACTTTATCTACAAATGTCAGACAAATCTCGTTTAATGAGTGAAGATTATGGCATTGAGAAAATTATTGAAGAATGGAAAG
CGATTTTATAA

Upstream 100 bases:

>100_bases
TGCTAATGCTCTTGCTAAACAGGGATATGATGTTTCTTTTATTAGCATTGGCGGTAACAAGCCTTTTTTCCAAGTTGATG
AAAAAATTAATATTTACGCA

Downstream 100 bases:

>100_bases
GGAATAATTTTATGTTAAAAAAATATTTGATCTCTTTAGATAAAGATATTCAACGTCGGGAATTATTCTTTTCTCAAAAA
AATACTGAGGATTTCCAAAT

Product: lipopolysaccharide biosynthesis protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MNKLPYSLKKDYFSITKKLRELVKELQLDTLIVVDGAIMLFSALALVNLNIKHILWEHYSFNFTGNRLVRTLGKYLAVTT
CDKIVTLTESEKTLWQEKFKTNNIISIANPNTLLPKNKLAKLENKTILSVGHLFSYKGFDYLLKVWQVLEKKYPNWNLKI
VGSGEEEENLKNLAKALDIEDSVNFIPRTNDVSFYYESSSIYCLPSQTEGLPLVLIEAMAFGLPIVAFNCSPGVKQLVEH
KENGFLCEQNNIEEMVKGLDLLINNPELYLQMSDKSRLMSEDYGIEKIIEEWKAIL

Sequences:

>Translated_296_residues
MNKLPYSLKKDYFSITKKLRELVKELQLDTLIVVDGAIMLFSALALVNLNIKHILWEHYSFNFTGNRLVRTLGKYLAVTT
CDKIVTLTESEKTLWQEKFKTNNIISIANPNTLLPKNKLAKLENKTILSVGHLFSYKGFDYLLKVWQVLEKKYPNWNLKI
VGSGEEEENLKNLAKALDIEDSVNFIPRTNDVSFYYESSSIYCLPSQTEGLPLVLIEAMAFGLPIVAFNCSPGVKQLVEH
KENGFLCEQNNIEEMVKGLDLLINNPELYLQMSDKSRLMSEDYGIEKIIEEWKAIL
>Mature_296_residues
MNKLPYSLKKDYFSITKKLRELVKELQLDTLIVVDGAIMLFSALALVNLNIKHILWEHYSFNFTGNRLVRTLGKYLAVTT
CDKIVTLTESEKTLWQEKFKTNNIISIANPNTLLPKNKLAKLENKTILSVGHLFSYKGFDYLLKVWQVLEKKYPNWNLKI
VGSGEEEENLKNLAKALDIEDSVNFIPRTNDVSFYYESSSIYCLPSQTEGLPLVLIEAMAFGLPIVAFNCSPGVKQLVEH
KENGFLCEQNNIEEMVKGLDLLINNPELYLQMSDKSRLMSEDYGIEKIIEEWKAIL

Specific function: Adds A Galactose Goup To A Glucose Group Of Lps. [C]

COG id: COG0438

COG function: function code M; Glycosyltransferase

Gene ontology:

Cell location: Membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI226510984, Length=249, Percent_Identity=22.8915662650602, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1788357, Length=134, Percent_Identity=30.5970149253731, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296 [H]

Pfam domain/function: PF00534 Glycos_transf_1 [H]

EC number: 2.4.1.-

Molecular weight: Translated: 33953; Mature: 33953

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKLPYSLKKDYFSITKKLRELVKELQLDTLIVVDGAIMLFSALALVNLNIKHILWEHYS
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHCCCHHHHHHHHEE
FNFTGNRLVRTLGKYLAVTTCDKIVTLTESEKTLWQEKFKTNNIISIANPNTLLPKNKLA
ECCCHHHHHHHHHHHHHHHHHHHEEEEECCHHHHHHHHHCCCCEEEECCCCCCCCCHHHH
KLENKTILSVGHLFSYKGFDYLLKVWQVLEKKYPNWNLKIVGSGEEEENLKNLAKALDIE
HHCCCEEEEHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHCCC
DSVNFIPRTNDVSFYYESSSIYCLPSQTEGLPLVLIEAMAFGLPIVAFNCSPGVKQLVEH
CCCCCCCCCCCEEEEEECCEEEEECCCCCCCHHHHHHHHHHCCCEEEEECCHHHHHHHHH
KENGFLCEQNNIEEMVKGLDLLINNPELYLQMSDKSRLMSEDYGIEKIIEEWKAIL
CCCCEEECCCCHHHHHHHHHEEECCCEEEEEECCHHHHHHHCCCHHHHHHHHHHCC
>Mature Secondary Structure
MNKLPYSLKKDYFSITKKLRELVKELQLDTLIVVDGAIMLFSALALVNLNIKHILWEHYS
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHCCCHHHHHHHHEE
FNFTGNRLVRTLGKYLAVTTCDKIVTLTESEKTLWQEKFKTNNIISIANPNTLLPKNKLA
ECCCHHHHHHHHHHHHHHHHHHHEEEEECCHHHHHHHHHCCCCEEEECCCCCCCCCHHHH
KLENKTILSVGHLFSYKGFDYLLKVWQVLEKKYPNWNLKIVGSGEEEENLKNLAKALDIE
HHCCCEEEEHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHCCC
DSVNFIPRTNDVSFYYESSSIYCLPSQTEGLPLVLIEAMAFGLPIVAFNCSPGVKQLVEH
CCCCCCCCCCCEEEEEECCEEEEECCCCCCCHHHHHHHHHHCCCEEEEECCHHHHHHHHH
KENGFLCEQNNIEEMVKGLDLLINNPELYLQMSDKSRLMSEDYGIEKIIEEWKAIL
CCCCEEECCCCHHHHHHHHHEEECCCEEEEEECCHHHHHHHCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Glycosyltransferases; Hexosyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]