Click here to switch to the map view.

The map label for this gene is nrdB [H]

Identifier: 148827082

GI number: 148827082

Start: 354277

End: 355506

Strand: Reverse

Name: nrdB [H]

Synonym: CGSHiGG_01995

Alternate gene names: 148827082

Gene position: 355506-354277 (Counterclockwise)

Preceding gene: 148827083

Following gene: 148827077

Centisome position: 18.84

GC content: 40.49

Gene sequence:

>1230_bases
ATGACCATTGAAATTCTTGTTCCAGACCTACCTGAATCAGTTGCGGATGCAACAGTGGCTACTTGGCATAAAAAAGTAGG
TGATACCGTTAAACGTGATGAAGTGATTGTTGAAATTGAAACGGATAAAGTTGTGCTAGAAGTGCCCGCACTTTCTGATG
GCGTACTGGCTGAAGTTGTTCAAGCTGAGGGTGAAACTGTGGTGAGTAAACAGCTGTTAGGAAAAATTTCTACGGCTCAA
GAAGGAGATGTCAGTTCAGCGACGTTAAAAGCAACGAATGAACCCACACCATCTGATCGTCAAAATGCAGCGATTGAAAA
TAGTCATAATCATAATGCGGATCAGAGCCCTGCAATTCGTCGTTTATTAGCAGAACATGATTTACAAGCAGATCAAATTC
AAGGTTCGGGTGTGGGTGGTCGTTTAACACGTGAAGATATTGAACGTGAAATTGCAAAACGACAAGCACAGCAAGTGAAA
CAAGAAGCTGCCACTGAGCAAAATACAATTAGTACGGTGGCTTATAGCGCACGTTCTGAAAAACGTGTGCCAATGACCCG
TTTGCGTAAACGTATTGCTGAACGTTTACTTGAAGCCAAAAATAGTACGGCGATGCTTACCACTTTCAATGAAGTGGATA
TGCAGCCGATAATGACTCTGCGTAAAACCTACGGCGAAAAATTTGAAAAACAACATGGTGTGCGTTTAGGATTTATGTCT
TTCTATATCAAAGCGGTTGTGGAAGCATTAAAACGTTATCCTGAAGTGAATGCTTCTATTGATGGGGATGATGTTGTTTA
CCATAACTATTTTGATATTAGTATTGCAGTTTCAACACCGCGTGGATTAGTCACACCAGTGCTTCGTGATTGCGATAAGC
TCAGTATGGCAGAGATCGAAAAACAAATTAAAGCATTAGCTGAAAAAGGTCGTGATGGCAAATTAACAGTAGAAGATCTT
ACTGGCGGCAATTTTACTATTACAAATGGTGGCGTGTTCGGTTCTCTTATGTCCACTCCAATTATCAATCCACCACAAAG
TGCGATTTTAGGAATGCACGCTATTAAAGAACGCCCGATTGCGCTTAATGGTCAAGTTGTGATTCGTCCTATGATGTACC
TTGCTTTATCTTACGATCATCGTTTAATTGACGGCCGTGAATCTGTTGGTTTCTTGGTAACGATTAAAGAATTATTGGAA
GATCCAACAAGATTGTTATTAGAAATCTAA

Upstream 100 bases:

>100_bases
ATTTTTATAAATTTACAGATGTACTTGTTGCATTCTGGATAAAAAGTAAAAGAGTAATTCAGTGCAATAAGTTGCACTTT
AAGAGATAAGGAAAAATAAA

Downstream 100 bases:

>100_bases
GAAATAAAAGGCTTTCTGTGTTAGAAAGCCTTTTAAAATGAACAAAAAATTAACCGCACTTTAAATATTTAAAAAGAATA
TTCCAAAGTGCGGTTAGTTT

Product: ribonucleotide-diphosphate reductase subunit beta

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 409; Mature: 408

Protein sequence:

>409_residues
MTIEILVPDLPESVADATVATWHKKVGDTVKRDEVIVEIETDKVVLEVPALSDGVLAEVVQAEGETVVSKQLLGKISTAQ
EGDVSSATLKATNEPTPSDRQNAAIENSHNHNADQSPAIRRLLAEHDLQADQIQGSGVGGRLTREDIEREIAKRQAQQVK
QEAATEQNTISTVAYSARSEKRVPMTRLRKRIAERLLEAKNSTAMLTTFNEVDMQPIMTLRKTYGEKFEKQHGVRLGFMS
FYIKAVVEALKRYPEVNASIDGDDVVYHNYFDISIAVSTPRGLVTPVLRDCDKLSMAEIEKQIKALAEKGRDGKLTVEDL
TGGNFTITNGGVFGSLMSTPIINPPQSAILGMHAIKERPIALNGQVVIRPMMYLALSYDHRLIDGRESVGFLVTIKELLE
DPTRLLLEI

Sequences:

>Translated_409_residues
MTIEILVPDLPESVADATVATWHKKVGDTVKRDEVIVEIETDKVVLEVPALSDGVLAEVVQAEGETVVSKQLLGKISTAQ
EGDVSSATLKATNEPTPSDRQNAAIENSHNHNADQSPAIRRLLAEHDLQADQIQGSGVGGRLTREDIEREIAKRQAQQVK
QEAATEQNTISTVAYSARSEKRVPMTRLRKRIAERLLEAKNSTAMLTTFNEVDMQPIMTLRKTYGEKFEKQHGVRLGFMS
FYIKAVVEALKRYPEVNASIDGDDVVYHNYFDISIAVSTPRGLVTPVLRDCDKLSMAEIEKQIKALAEKGRDGKLTVEDL
TGGNFTITNGGVFGSLMSTPIINPPQSAILGMHAIKERPIALNGQVVIRPMMYLALSYDHRLIDGRESVGFLVTIKELLE
DPTRLLLEI
>Mature_408_residues
TIEILVPDLPESVADATVATWHKKVGDTVKRDEVIVEIETDKVVLEVPALSDGVLAEVVQAEGETVVSKQLLGKISTAQE
GDVSSATLKATNEPTPSDRQNAAIENSHNHNADQSPAIRRLLAEHDLQADQIQGSGVGGRLTREDIEREIAKRQAQQVKQ
EAATEQNTISTVAYSARSEKRVPMTRLRKRIAERLLEAKNSTAMLTTFNEVDMQPIMTLRKTYGEKFEKQHGVRLGFMSF
YIKAVVEALKRYPEVNASIDGDDVVYHNYFDISIAVSTPRGLVTPVLRDCDKLSMAEIEKQIKALAEKGRDGKLTVEDLT
GGNFTITNGGVFGSLMSTPIINPPQSAILGMHAIKERPIALNGQVVIRPMMYLALSYDHRLIDGRESVGFLVTIKELLED
PTRLLLEI

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=234, Percent_Identity=58.5470085470086, Blast_Score=288, Evalue=7e-78,
Organism=Homo sapiens, GI203098753, Length=451, Percent_Identity=28.159645232816, Blast_Score=174, Evalue=2e-43,
Organism=Homo sapiens, GI203098816, Length=451, Percent_Identity=28.159645232816, Blast_Score=173, Evalue=2e-43,
Organism=Homo sapiens, GI31711992, Length=435, Percent_Identity=28.2758620689655, Blast_Score=171, Evalue=1e-42,
Organism=Homo sapiens, GI110671329, Length=433, Percent_Identity=26.7898383371824, Blast_Score=155, Evalue=6e-38,
Organism=Homo sapiens, GI260898739, Length=168, Percent_Identity=36.3095238095238, Blast_Score=111, Evalue=1e-24,
Organism=Escherichia coli, GI1786946, Length=411, Percent_Identity=73.9659367396594, Blast_Score=603, Evalue=1e-174,
Organism=Escherichia coli, GI1786305, Length=425, Percent_Identity=29.4117647058824, Blast_Score=162, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI25146366, Length=402, Percent_Identity=42.0398009950249, Blast_Score=314, Evalue=4e-86,
Organism=Caenorhabditis elegans, GI17560088, Length=441, Percent_Identity=30.1587301587302, Blast_Score=171, Evalue=8e-43,
Organism=Caenorhabditis elegans, GI17537937, Length=427, Percent_Identity=23.8875878220141, Blast_Score=155, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI17538894, Length=307, Percent_Identity=29.3159609120521, Blast_Score=132, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6320352, Length=405, Percent_Identity=43.2098765432099, Blast_Score=333, Evalue=2e-92,
Organism=Saccharomyces cerevisiae, GI6324258, Length=454, Percent_Identity=25.9911894273128, Blast_Score=151, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24645909, Length=225, Percent_Identity=60.4444444444444, Blast_Score=289, Evalue=2e-78,
Organism=Drosophila melanogaster, GI18859875, Length=431, Percent_Identity=28.3062645011601, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI20129315, Length=229, Percent_Identity=34.4978165938865, Blast_Score=141, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24582497, Length=229, Percent_Identity=34.4978165938865, Blast_Score=141, Evalue=1e-33,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 45121; Mature: 44990

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIEILVPDLPESVADATVATWHKKVGDTVKRDEVIVEIETDKVVLEVPALSDGVLAEVV
CEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCEEEEECCCCCCHHHHHHH
QAEGETVVSKQLLGKISTAQEGDVSSATLKATNEPTPSDRQNAAIENSHNHNADQSPAIR
HHCCCHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHH
RLLAEHDLQADQIQGSGVGGRLTREDIEREIAKRQAQQVKQEAATEQNTISTVAYSARSE
HHHHHCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
KRVPMTRLRKRIAERLLEAKNSTAMLTTFNEVDMQPIMTLRKTYGEKFEKQHGVRLGFMS
CCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHH
FYIKAVVEALKRYPEVNASIDGDDVVYHNYFDISIAVSTPRGLVTPVLRDCDKLSMAEIE
HHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEEEEECCCCHHHHHHHCCCCHHHHHHH
KQIKALAEKGRDGKLTVEDLTGGNFTITNGGVFGSLMSTPIINPPQSAILGMHAIKERPI
HHHHHHHHCCCCCCEEEEECCCCCEEEECCCHHHHHHHCCCCCCCHHHHHHHHHHHCCCE
ALNGQVVIRPMMYLALSYDHRLIDGRESVGFLVTIKELLEDPTRLLLEI
EECCCCHHHHHHHHHHHCCCCEECCCCCCCHHEEHHHHHCCHHHHHHCC
>Mature Secondary Structure 
TIEILVPDLPESVADATVATWHKKVGDTVKRDEVIVEIETDKVVLEVPALSDGVLAEVV
EEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCEEEEECCCCCCHHHHHHH
QAEGETVVSKQLLGKISTAQEGDVSSATLKATNEPTPSDRQNAAIENSHNHNADQSPAIR
HHCCCHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHH
RLLAEHDLQADQIQGSGVGGRLTREDIEREIAKRQAQQVKQEAATEQNTISTVAYSARSE
HHHHHCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
KRVPMTRLRKRIAERLLEAKNSTAMLTTFNEVDMQPIMTLRKTYGEKFEKQHGVRLGFMS
CCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHH
FYIKAVVEALKRYPEVNASIDGDDVVYHNYFDISIAVSTPRGLVTPVLRDCDKLSMAEIE
HHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEEEEECCCCHHHHHHHCCCCHHHHHHH
KQIKALAEKGRDGKLTVEDLTGGNFTITNGGVFGSLMSTPIINPPQSAILGMHAIKERPI
HHHHHHHHCCCCCCEEEEECCCCCEEEECCCHHHHHHHCCCCCCCHHHHHHHHHHHCCCE
ALNGQVVIRPMMYLALSYDHRLIDGRESVGFLVTIKELLEDPTRLLLEI
EECCCCHHHHHHHHHHHCCCCEECCCCCCCHHEEHHHHHCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]