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The map label for this gene is lpdA [H]

Identifier: 148827059

GI number: 148827059

Start: 326388

End: 327812

Strand: Direct

Name: lpdA [H]

Synonym: CGSHiGG_01850

Alternate gene names: 148827059

Gene position: 326388-327812 (Clockwise)

Preceding gene: 148827058

Following gene: 148827060

Centisome position: 17.29

GC content: 41.47

Gene sequence:

>1425_bases
ATGAGTAAAGAAATTAAAACCCAAGTCGTTGTACTTGGTGCAGGTCCTGCAGGTTATTCAGCTGCATTCCGTTGTGCGGA
TTTAGGATTAGAAACTGTTATTGTTGAGCGTTATTCAACCTTAGGTGGGGTGTGTTTAAACGTAGGTTGTATTCCATCTA
AAGCGTTATTACACGTTGCAAAAGTAATTGAAGAAGCAAAGCACGCTAATAAAAATGGTATTTATTTTAGTGAACCACGC
ATTGAGCTAGATGAAGTACGTGCAGGTAAAGAAGCTGTTGTTGCTAAATTAACTGGCGGTTTAGCGGGAATGGCAAAAGC
ACGTAAAGTGACTGTTGTTGAAGGTTTAGCGACTTTTACCGATTCTCACACATTAGTTGCGCGTGATCGCGATGGAAACC
CAACGACTGTTAAATTCGACAATGCCATTATTGCGGCAGGTTCTCGTCCAGTTCAATTACCCTTTATCCCACACGAAGAT
CCTCGTATTTGGGATTCAACAGATGCGCTCAAATTAAAAGAAGTACCGAAAAAACTACTTATTATGGGCGGTGGTATCAT
CGGTTTAGAAATGGGGACTGTATACAATGCTTTAGGTTCTGAAGTTGAAGTTGTTGAAATGTTCGACCAAGTGATTCCCG
CAGCAGATAAAGATGTTGTTGGTATTTACACTAAACAAGTTGAGAAAAAATTCAAGTTAATGCTTGAAACCAAAGTGACT
GCAGTTGAAGCAAAAGATGATGGTATCTACGTTTCAATGGAAGGCAAAGCCTGTAATGACACCAAACGTTATGATGCAGT
ACTTGTCGCTATCGGTCGTGTGCCAAATGGTAAATTGATTGATGCAGGTAAAGCAGGTGTTGAAGTGGATGATCGTGGTT
TTATTCACGTTGATAAACAAATGCGTACCAACGTGCCACATATCTACGCAATTGGGGATATTGTAGGTCAGCCAATGTTA
GCACATAAAGGTGTTAACGAAGGTCACGTTGCCGCAGAAGTCATTGCGGGACAAAAACACTACTTCGATCCTAAAGTAAT
TCCATCAATCGCTTATACCGAACCAGAAGTGGCGTGGGTAGGTAAAACTGAGAAAGAATGTAAACAAGAAGGCTTAAACT
ACGAAGTGGCTAAATTCCCTTGGGCTGCATCAGGCCGCGCGATTGCATCTGAATGTTCAGAAGGTATGACGAAGTTAATC
TTTGATAAAGATACACATCGTGTACTGGGCGGTGCAATCGTAGGTTCTAATGGCGGGGAATTATTAGGCGAAATCGGTCT
TGCAATTGAAATGGGTTGTGATGCAGAAGATATCGCATTAACTATCCACGCTCACCCAACTTTACACGAGTCTGTTGGCT
TAGCTGCGGAAGTATTTGAAGGCTCAATTACTGACCTGCCAAATGCAAAAGCGAAGAAAAAATAA

Upstream 100 bases:

>100_bases
TTTATGCCCACCGATTAATGAAATCTTTGGTGGGCAGAAACCCATCCTATAAAGACCGTAAAAGTGCGGTCAATATTTTG
AACGTTTTTACGAGGTAAAA

Downstream 100 bases:

>100_bases
TTCTTATTGAAGAATTTTTAATTCGGCTTGGATTAAAAATTCCAGCCTAAATAAAAATCCCCAATTGGGATTTTTATTTT
CCTCTCTTTCTATTTTTTTA

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes [H]

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MSKEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYSTLGGVCLNVGCIPSKALLHVAKVIEEAKHANKNGIYFSEPR
IELDEVRAGKEAVVAKLTGGLAGMAKARKVTVVEGLATFTDSHTLVARDRDGNPTTVKFDNAIIAAGSRPVQLPFIPHED
PRIWDSTDALKLKEVPKKLLIMGGGIIGLEMGTVYNALGSEVEVVEMFDQVIPAADKDVVGIYTKQVEKKFKLMLETKVT
AVEAKDDGIYVSMEGKACNDTKRYDAVLVAIGRVPNGKLIDAGKAGVEVDDRGFIHVDKQMRTNVPHIYAIGDIVGQPML
AHKGVNEGHVAAEVIAGQKHYFDPKVIPSIAYTEPEVAWVGKTEKECKQEGLNYEVAKFPWAASGRAIASECSEGMTKLI
FDKDTHRVLGGAIVGSNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHESVGLAAEVFEGSITDLPNAKAKKK

Sequences:

>Translated_474_residues
MSKEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYSTLGGVCLNVGCIPSKALLHVAKVIEEAKHANKNGIYFSEPR
IELDEVRAGKEAVVAKLTGGLAGMAKARKVTVVEGLATFTDSHTLVARDRDGNPTTVKFDNAIIAAGSRPVQLPFIPHED
PRIWDSTDALKLKEVPKKLLIMGGGIIGLEMGTVYNALGSEVEVVEMFDQVIPAADKDVVGIYTKQVEKKFKLMLETKVT
AVEAKDDGIYVSMEGKACNDTKRYDAVLVAIGRVPNGKLIDAGKAGVEVDDRGFIHVDKQMRTNVPHIYAIGDIVGQPML
AHKGVNEGHVAAEVIAGQKHYFDPKVIPSIAYTEPEVAWVGKTEKECKQEGLNYEVAKFPWAASGRAIASECSEGMTKLI
FDKDTHRVLGGAIVGSNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHESVGLAAEVFEGSITDLPNAKAKKK
>Mature_473_residues
SKEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYSTLGGVCLNVGCIPSKALLHVAKVIEEAKHANKNGIYFSEPRI
ELDEVRAGKEAVVAKLTGGLAGMAKARKVTVVEGLATFTDSHTLVARDRDGNPTTVKFDNAIIAAGSRPVQLPFIPHEDP
RIWDSTDALKLKEVPKKLLIMGGGIIGLEMGTVYNALGSEVEVVEMFDQVIPAADKDVVGIYTKQVEKKFKLMLETKVTA
VEAKDDGIYVSMEGKACNDTKRYDAVLVAIGRVPNGKLIDAGKAGVEVDDRGFIHVDKQMRTNVPHIYAIGDIVGQPMLA
HKGVNEGHVAAEVIAGQKHYFDPKVIPSIAYTEPEVAWVGKTEKECKQEGLNYEVAKFPWAASGRAIASECSEGMTKLIF
DKDTHRVLGGAIVGSNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHESVGLAAEVFEGSITDLPNAKAKKK

Specific function: Lipoamide dehydrogenase is a component of the alpha- ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=458, Percent_Identity=43.0131004366812, Blast_Score=341, Evalue=8e-94,
Organism=Homo sapiens, GI50301238, Length=456, Percent_Identity=27.1929824561404, Blast_Score=145, Evalue=8e-35,
Organism=Homo sapiens, GI22035672, Length=429, Percent_Identity=29.6037296037296, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI291045266, Length=434, Percent_Identity=29.0322580645161, Blast_Score=122, Evalue=5e-28,
Organism=Homo sapiens, GI148277065, Length=437, Percent_Identity=27.2311212814645, Blast_Score=119, Evalue=7e-27,
Organism=Homo sapiens, GI33519430, Length=437, Percent_Identity=27.2311212814645, Blast_Score=119, Evalue=7e-27,
Organism=Homo sapiens, GI33519428, Length=437, Percent_Identity=27.2311212814645, Blast_Score=119, Evalue=7e-27,
Organism=Homo sapiens, GI33519426, Length=437, Percent_Identity=27.2311212814645, Blast_Score=119, Evalue=7e-27,
Organism=Homo sapiens, GI148277071, Length=437, Percent_Identity=27.2311212814645, Blast_Score=119, Evalue=8e-27,
Organism=Homo sapiens, GI291045268, Length=426, Percent_Identity=27.6995305164319, Blast_Score=108, Evalue=1e-23,
Organism=Escherichia coli, GI1786307, Length=475, Percent_Identity=81.4736842105263, Blast_Score=792, Evalue=0.0,
Organism=Escherichia coli, GI87082354, Length=467, Percent_Identity=28.9079229122056, Blast_Score=199, Evalue=3e-52,
Organism=Escherichia coli, GI87081717, Length=457, Percent_Identity=28.4463894967177, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI1789915, Length=440, Percent_Identity=26.8181818181818, Blast_Score=137, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI32565766, Length=447, Percent_Identity=41.6107382550336, Blast_Score=338, Evalue=4e-93,
Organism=Caenorhabditis elegans, GI17557007, Length=472, Percent_Identity=28.6016949152542, Blast_Score=138, Evalue=7e-33,
Organism=Caenorhabditis elegans, GI71983429, Length=436, Percent_Identity=24.3119266055046, Blast_Score=113, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI71983419, Length=436, Percent_Identity=24.3119266055046, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI71982272, Length=436, Percent_Identity=25.2293577981651, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17559934, Length=146, Percent_Identity=30.8219178082192, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6321091, Length=456, Percent_Identity=42.3245614035088, Blast_Score=319, Evalue=5e-88,
Organism=Saccharomyces cerevisiae, GI6325240, Length=468, Percent_Identity=27.5641025641026, Blast_Score=186, Evalue=9e-48,
Organism=Saccharomyces cerevisiae, GI6325166, Length=454, Percent_Identity=27.9735682819383, Blast_Score=161, Evalue=2e-40,
Organism=Drosophila melanogaster, GI21358499, Length=450, Percent_Identity=41.7777777777778, Blast_Score=333, Evalue=1e-91,
Organism=Drosophila melanogaster, GI24640549, Length=466, Percent_Identity=29.6137339055794, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24640553, Length=466, Percent_Identity=29.6137339055794, Blast_Score=126, Evalue=4e-29,
Organism=Drosophila melanogaster, GI24640551, Length=466, Percent_Identity=29.6137339055794, Blast_Score=125, Evalue=6e-29,
Organism=Drosophila melanogaster, GI17737741, Length=466, Percent_Identity=27.0386266094421, Blast_Score=105, Evalue=5e-23,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 50704; Mature: 50573

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYSTLGGVCLNVGCIPSKALLHVA
CCCCCEEEEEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHHCCEEEEECCCCHHHHHHHH
KVIEEAKHANKNGIYFSEPRIELDEVRAGKEAVVAKLTGGLAGMAKARKVTVVEGLATFT
HHHHHHHHCCCCCEEECCCCCCHHHHCCCHHHHHHHHHCCHHHHHHCCEEEEEHHHHHHC
DSHTLVARDRDGNPTTVKFDNAIIAAGSRPVQLPFIPHEDPRIWDSTDALKLKEVPKKLL
CCCEEEEECCCCCCCEEEECCEEEECCCCCEEECCCCCCCCCCCCCCCCHHHHHCCCEEE
IMGGGIIGLEMGTVYNALGSEVEVVEMFDQVIPAADKDVVGIYTKQVEKKFKLMLETKVT
EECCCEEEEEHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHEEEEEE
AVEAKDDGIYVSMEGKACNDTKRYDAVLVAIGRVPNGKLIDAGKAGVEVDDRGFIHVDKQ
EEEECCCCEEEEECCCCCCCCHHHCEEEEEEECCCCCCEEECCCCCCEECCCCEEEECHH
MRTNVPHIYAIGDIVGQPMLAHKGVNEGHVAAEVIAGQKHYFDPKVIPSIAYTEPEVAWV
HHCCCCEEEEEHHHHCCCHHHHCCCCCCCHHHHHHCCCCCCCCCCCCCCEECCCCCEEEE
GKTEKECKQEGLNYEVAKFPWAASGRAIASECSEGMTKLIFDKDTHRVLGGAIVGSNGGE
CCCHHHHHHCCCCEEEEECCCCCCCCHHHHHHHCCCCEEEECCCCHHEECEEEECCCCCH
LLGEIGLAIEMGCDAEDIALTIHAHPTLHESVGLAAEVFEGSITDLPNAKAKKK
HHHHCCEEEEECCCCCCEEEEEECCCCHHHHHCCHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SKEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYSTLGGVCLNVGCIPSKALLHVA
CCCCEEEEEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHHCCEEEEECCCCHHHHHHHH
KVIEEAKHANKNGIYFSEPRIELDEVRAGKEAVVAKLTGGLAGMAKARKVTVVEGLATFT
HHHHHHHHCCCCCEEECCCCCCHHHHCCCHHHHHHHHHCCHHHHHHCCEEEEEHHHHHHC
DSHTLVARDRDGNPTTVKFDNAIIAAGSRPVQLPFIPHEDPRIWDSTDALKLKEVPKKLL
CCCEEEEECCCCCCCEEEECCEEEECCCCCEEECCCCCCCCCCCCCCCCHHHHHCCCEEE
IMGGGIIGLEMGTVYNALGSEVEVVEMFDQVIPAADKDVVGIYTKQVEKKFKLMLETKVT
EECCCEEEEEHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHEEEEEE
AVEAKDDGIYVSMEGKACNDTKRYDAVLVAIGRVPNGKLIDAGKAGVEVDDRGFIHVDKQ
EEEECCCCEEEEECCCCCCCCHHHCEEEEEEECCCCCCEEECCCCCCEECCCCEEEECHH
MRTNVPHIYAIGDIVGQPMLAHKGVNEGHVAAEVIAGQKHYFDPKVIPSIAYTEPEVAWV
HHCCCCEEEEEHHHHCCCHHHHCCCCCCCHHHHHHCCCCCCCCCCCCCCEECCCCCEEEE
GKTEKECKQEGLNYEVAKFPWAASGRAIASECSEGMTKLIFDKDTHRVLGGAIVGSNGGE
CCCHHHHHHCCCCEEEEECCCCCCCCHHHHHHHCCCCEEEECCCCHHEECEEEECCCCCH
LLGEIGLAIEMGCDAEDIALTIHAHPTLHESVGLAAEVFEGSITDLPNAKAKKK
HHHHCCEEEEECCCCCCEEEEEECCCCHHHHHCCHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]