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The map label for this gene is gloB

Identifier: 148827029

GI number: 148827029

Start: 277368

End: 278099

Strand: Reverse

Name: gloB

Synonym: CGSHiGG_01615

Alternate gene names: 148827029

Gene position: 278099-277368 (Counterclockwise)

Preceding gene: 148827030

Following gene: 148827027

Centisome position: 14.74

GC content: 40.3

Gene sequence:

>732_bases
ATGTTATTTGCTTTACCTGCGCTGAATGATAACTACATTTGGCTTTATCAACGAGAAAATCTGCCGCTTATTATTGTCGA
TTTGCCTGAAACGGACAAGCTGTTTGCATGGCTAGATAAGCAAAACGCAACGGTTGAAGCGGTGTTGCTTACTCACGAAC
ATGATGATCATACACAAGGTGTGTCGGCATTTAAAAAACGTTATCCAACAGTGCCGATTTATGGGCCGCAAGAATGTGAG
AAAAAAGGTGCGACTCAAATTGTGAACGAAGGGAAGATCCTTACCGCAAATTATCAAATTGATGTCATTCCGACGGGGGG
ACACACTAAACAGCACGTTAGTTTTTTAGTGGATAATCATTTATTTTGTGGCGATGCGTTATTTTCTGCTGGTTGCGGAC
GTGTTTTCACAGGTAATTATGCGCTGATGTTTGAAGGTTTACAGCGTTTGAATACATTGCCTGACGAGACGATTGTTTGC
CCAGCTCATGAATATACCTTGGGGAATTTAGCATTTGCGGAAACTGTGCTGGTGGATAAAAGTGCGGTAGAAAAAAGTGC
GGTAGAAAAAAGTGCGGTAGAAAAACAACGTATTTTTGTTGAAACACAACGGGCTGAAAATAAACCGAGTTTGCCAACAA
CCTTAAAACTAGAGCGAGAAATTAATCCGTTTTTACAAGCAAAAACACTTGAAGAATTTACCGCACTTCGCAAAGCAAAA
GATATTTTCTAA

Upstream 100 bases:

>100_bases
CGGACAGAAAAAATGGGCGTTAATTTGGTGGAATGTGGATCGCATCTAATGTTTCAAGTGGAATCAATTTATTTTTCTAT
CACTAATTAAAAGGACGATT

Downstream 100 bases:

>100_bases
GTTACTTTTTATTGGAGGGACGCAAAGTGCGGTGATTTTTTAAATTAGCAGATTATTTCTTTCTCGCTAACATGGTGGCA
AATTTCATTTTAATGCGATT

Product: hydroxyacylglutathione hydrolase

Products: NA

Alternate protein names: Glyoxalase II; Glx II

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MLFALPALNDNYIWLYQRENLPLIIVDLPETDKLFAWLDKQNATVEAVLLTHEHDDHTQGVSAFKKRYPTVPIYGPQECE
KKGATQIVNEGKILTANYQIDVIPTGGHTKQHVSFLVDNHLFCGDALFSAGCGRVFTGNYALMFEGLQRLNTLPDETIVC
PAHEYTLGNLAFAETVLVDKSAVEKSAVEKSAVEKQRIFVETQRAENKPSLPTTLKLEREINPFLQAKTLEEFTALRKAK
DIF

Sequences:

>Translated_243_residues
MLFALPALNDNYIWLYQRENLPLIIVDLPETDKLFAWLDKQNATVEAVLLTHEHDDHTQGVSAFKKRYPTVPIYGPQECE
KKGATQIVNEGKILTANYQIDVIPTGGHTKQHVSFLVDNHLFCGDALFSAGCGRVFTGNYALMFEGLQRLNTLPDETIVC
PAHEYTLGNLAFAETVLVDKSAVEKSAVEKSAVEKQRIFVETQRAENKPSLPTTLKLEREINPFLQAKTLEEFTALRKAK
DIF
>Mature_243_residues
MLFALPALNDNYIWLYQRENLPLIIVDLPETDKLFAWLDKQNATVEAVLLTHEHDDHTQGVSAFKKRYPTVPIYGPQECE
KKGATQIVNEGKILTANYQIDVIPTGGHTKQHVSFLVDNHLFCGDALFSAGCGRVFTGNYALMFEGLQRLNTLPDETIVC
PAHEYTLGNLAFAETVLVDKSAVEKSAVEKSAVEKQRIFVETQRAENKPSLPTTLKLEREINPFLQAKTLEEFTALRKAK
DIF

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family

Homologues:

Organism=Homo sapiens, GI116642887, Length=238, Percent_Identity=33.1932773109244, Blast_Score=125, Evalue=4e-29,
Organism=Homo sapiens, GI21703352, Length=238, Percent_Identity=33.1932773109244, Blast_Score=125, Evalue=5e-29,
Organism=Homo sapiens, GI14150041, Length=249, Percent_Identity=28.5140562248996, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI94538320, Length=260, Percent_Identity=29.6153846153846, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI94538322, Length=260, Percent_Identity=29.6153846153846, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI46361987, Length=180, Percent_Identity=32.2222222222222, Blast_Score=92, Evalue=4e-19,
Organism=Escherichia coli, GI1786406, Length=259, Percent_Identity=37.0656370656371, Blast_Score=168, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI17536925, Length=237, Percent_Identity=31.2236286919831, Blast_Score=104, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6320478, Length=236, Percent_Identity=28.3898305084746, Blast_Score=70, Evalue=4e-13,
Organism=Drosophila melanogaster, GI21356335, Length=263, Percent_Identity=29.277566539924, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24667711, Length=263, Percent_Identity=29.277566539924, Blast_Score=105, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24667703, Length=263, Percent_Identity=29.277566539924, Blast_Score=105, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLO2_HAEIG (A5UF43)

Other databases:

- EMBL:   CP000672
- RefSeq:   YP_001291782.1
- ProteinModelPortal:   A5UF43
- SMR:   A5UF43
- STRING:   A5UF43
- GeneID:   5226872
- GenomeReviews:   CP000672_GR
- KEGG:   hiq:CGSHiGG_01615
- eggNOG:   COG0491
- HOGENOM:   HBG753931
- OMA:   WCAHEYT
- ProtClustDB:   CLSK870202
- HAMAP:   MF_01374
- InterPro:   IPR001279
- InterPro:   IPR017782
- SMART:   SM00849
- TIGRFAMs:   TIGR03413

Pfam domain/function: PF00753 Lactamase_B

EC number: =3.1.2.6

Molecular weight: Translated: 27337; Mature: 27337

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFALPALNDNYIWLYQRENLPLIIVDLPETDKLFAWLDKQNATVEAVLLTHEHDDHTQG
CEEEECCCCCCEEEEEEECCCCEEEEECCCCCCEEEEECCCCCEEEEEEEEECCCCHHHH
VSAFKKRYPTVPIYGPQECEKKGATQIVNEGKILTANYQIDVIPTGGHTKQHVSFLVDNH
HHHHHHHCCCCCCCCCHHHHHCCCHHHHCCCEEEEEEEEEEEEECCCCCHHHHHHHHCCC
LFCGDALFSAGCGRVFTGNYALMFEGLQRLNTLPDETIVCPAHEYTLGNLAFAETVLVDK
EEECHHHHHCCCCCEEECCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHCH
SAVEKSAVEKSAVEKQRIFVETQRAENKPSLPTTLKLEREINPFLQAKTLEEFTALRKAK
HHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHH
DIF
CCC
>Mature Secondary Structure
MLFALPALNDNYIWLYQRENLPLIIVDLPETDKLFAWLDKQNATVEAVLLTHEHDDHTQG
CEEEECCCCCCEEEEEEECCCCEEEEECCCCCCEEEEECCCCCEEEEEEEEECCCCHHHH
VSAFKKRYPTVPIYGPQECEKKGATQIVNEGKILTANYQIDVIPTGGHTKQHVSFLVDNH
HHHHHHHCCCCCCCCCHHHHHCCCHHHHCCCEEEEEEEEEEEEECCCCCHHHHHHHHCCC
LFCGDALFSAGCGRVFTGNYALMFEGLQRLNTLPDETIVCPAHEYTLGNLAFAETVLVDK
EEECHHHHHCCCCCEEECCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHCH
SAVEKSAVEKSAVEKQRIFVETQRAENKPSLPTTLKLEREINPFLQAKTLEEFTALRKAK
HHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHH
DIF
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA