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The map label for this gene is cobB [H]

Identifier: 148826987

GI number: 148826987

Start: 232107

End: 232766

Strand: Reverse

Name: cobB [H]

Synonym: CGSHiGG_01365

Alternate gene names: 148826987

Gene position: 232766-232107 (Counterclockwise)

Preceding gene: 148826988

Following gene: 148826986

Centisome position: 12.33

GC content: 42.42

Gene sequence:

>660_bases
ATGACAGAGAAAAATAAACCAATTTGCGTGGTATTAACGGGAGCTGGCATTAGTGCCGAAAGTGGAATTCCAACTTTTAG
ATCGGAAGATGGATTGTGGGCAGGGCATAAAGTAGAAGAAGTTTGCACGCCCGAAGCCTTGCAAAAGAACCGTGCGAAAG
TGCTTGATTTCTATAACCAACGCCGTAAAAATGCGGCAGCAGCTAAGCCAAACGCTGCGCATCTCGCCTTAGTTGAACTA
GAAAAAGCCTATGATGTGAGAATCATCACGCAAAATGTGGATGATTTACATGAACGTGCCGGCAGCTCGAAGGTGTTGCA
TTTACACGGTGAATTAAATAAAGCTCGCAGTAGCTTTGATGAAAGTTATATTGTGGATTGTTTTGGTGATCAGAAATTAG
AAGATAAAGATCCAAATGGGCACCCAATGCGCCCTTACATCGTCTTTTTTGGTGAAATGGTGCCGATGCTAGAACGAGCG
GTTGATATTGTGGAACAAGCAGATGTTGTGTTAGTGATTGGCACTTCTTTACAAGTGTATCCAGCCAATGGCTTAGTCAA
TGAAGCCCCAAGAAAAGCGCCAATTTATCTAATTGATCCTAACCCAAATACAGGATTTGTTCGTAAGCAAGTTATTGCAA
TCAAAGAAAAAGCAGGCTAG

Upstream 100 bases:

>100_bases
TATTTAATTGATGATCGCACTAAAAATGGAGCTGGTAAATTTCAAGGCGAACATGTTCATTTTGGTACAGAACAGTTTGC
TAATAAAAGGAGCCTGAAAA

Downstream 100 bases:

>100_bases
GGTGTGCCAAAAGTGGTGGCAGAGTTATTAGAGAACACCAAAAACTCATAGAAAAATGACCGCACTGTTTAAACAAATAG
CGACTTCGGTCGCTATTTAT

Product: NAD-dependent deacetylase

Products: NA

Alternate protein names: Regulatory protein SIR2 homolog [H]

Number of amino acids: Translated: 219; Mature: 218

Protein sequence:

>219_residues
MTEKNKPICVVLTGAGISAESGIPTFRSEDGLWAGHKVEEVCTPEALQKNRAKVLDFYNQRRKNAAAAKPNAAHLALVEL
EKAYDVRIITQNVDDLHERAGSSKVLHLHGELNKARSSFDESYIVDCFGDQKLEDKDPNGHPMRPYIVFFGEMVPMLERA
VDIVEQADVVLVIGTSLQVYPANGLVNEAPRKAPIYLIDPNPNTGFVRKQVIAIKEKAG

Sequences:

>Translated_219_residues
MTEKNKPICVVLTGAGISAESGIPTFRSEDGLWAGHKVEEVCTPEALQKNRAKVLDFYNQRRKNAAAAKPNAAHLALVEL
EKAYDVRIITQNVDDLHERAGSSKVLHLHGELNKARSSFDESYIVDCFGDQKLEDKDPNGHPMRPYIVFFGEMVPMLERA
VDIVEQADVVLVIGTSLQVYPANGLVNEAPRKAPIYLIDPNPNTGFVRKQVIAIKEKAG
>Mature_218_residues
TEKNKPICVVLTGAGISAESGIPTFRSEDGLWAGHKVEEVCTPEALQKNRAKVLDFYNQRRKNAAAAKPNAAHLALVELE
KAYDVRIITQNVDDLHERAGSSKVLHLHGELNKARSSFDESYIVDCFGDQKLEDKDPNGHPMRPYIVFFGEMVPMLERAV
DIVEQADVVLVIGTSLQVYPANGLVNEAPRKAPIYLIDPNPNTGFVRKQVIAIKEKAG

Specific function: Modulates the activities of several enzymes which are inactive in their acetylated form [H]

COG id: COG0846

COG function: function code K; NAD-dependent protein deacetylases, SIR2 family

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 deacetylase sirtuin-type domain [H]

Homologues:

Organism=Homo sapiens, GI300795542, Length=201, Percent_Identity=36.318407960199, Blast_Score=126, Evalue=1e-29,
Organism=Homo sapiens, GI13787215, Length=204, Percent_Identity=35.7843137254902, Blast_Score=117, Evalue=8e-27,
Organism=Homo sapiens, GI6912664, Length=204, Percent_Identity=35.7843137254902, Blast_Score=117, Evalue=9e-27,
Organism=Homo sapiens, GI7657575, Length=214, Percent_Identity=32.7102803738318, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI6912660, Length=211, Percent_Identity=33.175355450237, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI63054862, Length=207, Percent_Identity=32.3671497584541, Blast_Score=71, Evalue=9e-13,
Organism=Escherichia coli, GI308199517, Length=191, Percent_Identity=48.6910994764398, Blast_Score=169, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI17567771, Length=238, Percent_Identity=29.4117647058824, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17541892, Length=210, Percent_Identity=29.5238095238095, Blast_Score=73, Evalue=9e-14,
Organism=Drosophila melanogaster, GI28571445, Length=130, Percent_Identity=38.4615384615385, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24648389, Length=195, Percent_Identity=30.7692307692308, Blast_Score=67, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003000 [H]

Pfam domain/function: PF02146 SIR2 [H]

EC number: 3.5.1.- [C]

Molecular weight: Translated: 24190; Mature: 24059

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS50305 SIRTUIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEKNKPICVVLTGAGISAESGIPTFRSEDGLWAGHKVEEVCTPEALQKNRAKVLDFYNQ
CCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHH
RRKNAAAAKPNAAHLALVELEKAYDVRIITQNVDDLHERAGSSKVLHLHGELNKARSSFD
HHCCCCCCCCCCCEEEEEEECCCCEEEEEECCHHHHHHHCCCCEEEEEECCHHHHHHCCC
ESYIVDCFGDQKLEDKDPNGHPMRPYIVFFGEMVPMLERAVDIVEQADVVLVIGTSLQVY
CCEEEEECCCCCCCCCCCCCCCCCHHEEEHHHHHHHHHHHHHHHHHCCEEEEECCEEEEE
PANGLVNEAPRKAPIYLIDPNPNTGFVRKQVIAIKEKAG
ECCCCCCCCCCCCCEEEECCCCCCCHHHHHEEEEECCCC
>Mature Secondary Structure 
TEKNKPICVVLTGAGISAESGIPTFRSEDGLWAGHKVEEVCTPEALQKNRAKVLDFYNQ
CCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHH
RRKNAAAAKPNAAHLALVELEKAYDVRIITQNVDDLHERAGSSKVLHLHGELNKARSSFD
HHCCCCCCCCCCCEEEEEEECCCCEEEEEECCHHHHHHHCCCCEEEEEECCHHHHHHCCC
ESYIVDCFGDQKLEDKDPNGHPMRPYIVFFGEMVPMLERAVDIVEQADVVLVIGTSLQVY
CCEEEEECCCCCCCCCCCCCCCCCHHEEEHHHHHHHHHHHHHHHHHCCEEEEECCEEEEE
PANGLVNEAPRKAPIYLIDPNPNTGFVRKQVIAIKEKAG
ECCCCCCCCCCCCCEEEECCCCCCCHHHHHEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10024565 [H]