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The map label for this gene is rlmE [H]

Identifier: 148826967

GI number: 148826967

Start: 207197

End: 207784

Strand: Reverse

Name: rlmE [H]

Synonym: CGSHiGG_01225

Alternate gene names: 148826967

Gene position: 207784-207197 (Counterclockwise)

Preceding gene: 148826969

Following gene: 148826966

Centisome position: 11.01

GC content: 38.95

Gene sequence:

>588_bases
ATGAACATTTTTAGTGATCAATTTGTGCAAAAAGCACATAAGCAAAAGTTGCGATCACGCGCTTATTTTAAGATTGATGA
AATTCAGCAAACGGATAAATTATTTAAACAGGGAATGACAGTGGTCGATCTCGGTGCCGCACCAGGCGGTTGGTCACAAT
ACGTAGTAAGCCAAATTGGTGGCAAAGGTCGAGTGATTGCTTGTGATATTTTAGAAATGGCTCCCATTGTAGGCGTTGAT
TTTCTACAAGGTGATTTCCGTGATGAAAATGTTTTGAATGCTTTATTAGCACGCGTAGGCGAAGATAAAGTTGATGTTGT
GATGTCTGATATGGCTCCCAATTTTAGTGGAATGCCATCAGTGGATATTCCACGTGCAATGTATTTGGTCGAGCTTGCTT
TAGATATGTGTAAACAAGTCTTGGCAAGCAAAGGAAGTTTTGTTGTTAAAGTTTTCCAAGGGGAAGGCTTTGATGAATAT
TTAAGAGAAATTCGTTCTTTATTTAATGTGGTAAAAGTGCGTAAGCCTGAAGCATCACGTGGACGATCTCGTGAAGTATA
TATTGTGGCAACAGGCTATAAAGGATAA

Upstream 100 bases:

>100_bases
TGAATTTGACAGATTGTACCCAAATATAGGCGAAACGACTACAAAAAATAGAAGATTATGGGAAAGAAAAAACGTTCAGC
GAGTTCTTCTCGTTGGCTGA

Downstream 100 bases:

>100_bases
TTGCTTTGATAGCTTTTCATTGCTAATGATGTGTAGTATCTTGTGACGAAATTTTTTAACTTAGGAAAGGTAGGTTAGAC
TTTGAACGATATGGTCAAAA

Product: cell division protein

Products: NA

Alternate protein names: 23S rRNA Um2552 methyltransferase; rRNA (uridine-2'-O-)-methyltransferase [H]

Number of amino acids: Translated: 195; Mature: 195

Protein sequence:

>195_residues
MNIFSDQFVQKAHKQKLRSRAYFKIDEIQQTDKLFKQGMTVVDLGAAPGGWSQYVVSQIGGKGRVIACDILEMAPIVGVD
FLQGDFRDENVLNALLARVGEDKVDVVMSDMAPNFSGMPSVDIPRAMYLVELALDMCKQVLASKGSFVVKVFQGEGFDEY
LREIRSLFNVVKVRKPEASRGRSREVYIVATGYKG

Sequences:

>Translated_195_residues
MNIFSDQFVQKAHKQKLRSRAYFKIDEIQQTDKLFKQGMTVVDLGAAPGGWSQYVVSQIGGKGRVIACDILEMAPIVGVD
FLQGDFRDENVLNALLARVGEDKVDVVMSDMAPNFSGMPSVDIPRAMYLVELALDMCKQVLASKGSFVVKVFQGEGFDEY
LREIRSLFNVVKVRKPEASRGRSREVYIVATGYKG
>Mature_195_residues
MNIFSDQFVQKAHKQKLRSRAYFKIDEIQQTDKLFKQGMTVVDLGAAPGGWSQYVVSQIGGKGRVIACDILEMAPIVGVD
FLQGDFRDENVLNALLARVGEDKVDVVMSDMAPNFSGMPSVDIPRAMYLVELALDMCKQVLASKGSFVVKVFQGEGFDEY
LREIRSLFNVVKVRKPEASRGRSREVYIVATGYKG

Specific function: Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit [H]

COG id: COG0293

COG function: function code J; 23S rRNA methylase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI7019377, Length=199, Percent_Identity=33.6683417085427, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI29029591, Length=190, Percent_Identity=33.1578947368421, Blast_Score=106, Evalue=1e-23,
Organism=Homo sapiens, GI29029589, Length=190, Percent_Identity=33.1578947368421, Blast_Score=106, Evalue=1e-23,
Organism=Homo sapiens, GI7110661, Length=190, Percent_Identity=33.1578947368421, Blast_Score=106, Evalue=1e-23,
Organism=Homo sapiens, GI194097365, Length=188, Percent_Identity=31.9148936170213, Blast_Score=102, Evalue=2e-22,
Organism=Escherichia coli, GI1789569, Length=191, Percent_Identity=81.151832460733, Blast_Score=321, Evalue=2e-89,
Organism=Caenorhabditis elegans, GI17553474, Length=202, Percent_Identity=34.1584158415842, Blast_Score=109, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI17554650, Length=191, Percent_Identity=30.3664921465969, Blast_Score=99, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI71987550, Length=189, Percent_Identity=30.6878306878307, Blast_Score=89, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71987561, Length=189, Percent_Identity=30.6878306878307, Blast_Score=89, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17553860, Length=189, Percent_Identity=30.6878306878307, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6319535, Length=200, Percent_Identity=32.5, Blast_Score=103, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6319796, Length=191, Percent_Identity=29.8429319371728, Blast_Score=88, Evalue=1e-18,
Organism=Drosophila melanogaster, GI18859957, Length=188, Percent_Identity=34.5744680851064, Blast_Score=103, Evalue=6e-23,
Organism=Drosophila melanogaster, GI24648639, Length=193, Percent_Identity=33.6787564766839, Blast_Score=100, Evalue=9e-22,
Organism=Drosophila melanogaster, GI21356387, Length=201, Percent_Identity=31.3432835820896, Blast_Score=96, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24647580, Length=198, Percent_Identity=28.2828282828283, Blast_Score=91, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015507
- InterPro:   IPR004512
- InterPro:   IPR002877 [H]

Pfam domain/function: PF01728 FtsJ [H]

EC number: =2.1.1.166 [H]

Molecular weight: Translated: 21737; Mature: 21737

Theoretical pI: Translated: 8.65; Mature: 8.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIFSDQFVQKAHKQKLRSRAYFKIDEIQQTDKLFKQGMTVVDLGAAPGGWSQYVVSQIG
CCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHCC
GKGRVIACDILEMAPIVGVDFLQGDFRDENVLNALLARVGEDKVDVVMSDMAPNFSGMPS
CCCCEEEEHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCC
VDIPRAMYLVELALDMCKQVLASKGSFVVKVFQGEGFDEYLREIRSLFNVVKVRKPEASR
CCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCC
GRSREVYIVATGYKG
CCCCEEEEEEECCCC
>Mature Secondary Structure
MNIFSDQFVQKAHKQKLRSRAYFKIDEIQQTDKLFKQGMTVVDLGAAPGGWSQYVVSQIG
CCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHCC
GKGRVIACDILEMAPIVGVDFLQGDFRDENVLNALLARVGEDKVDVVMSDMAPNFSGMPS
CCCCEEEEHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCC
VDIPRAMYLVELALDMCKQVLASKGSFVVKVFQGEGFDEYLREIRSLFNVVKVRKPEASR
CCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCC
GRSREVYIVATGYKG
CCCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA