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The map label for this gene is folE [H]

Identifier: 148826950

GI number: 148826950

Start: 191948

End: 192604

Strand: Reverse

Name: folE [H]

Synonym: CGSHiGG_01120

Alternate gene names: 148826950

Gene position: 192604-191948 (Counterclockwise)

Preceding gene: 148826954

Following gene: 148826949

Centisome position: 10.21

GC content: 36.99

Gene sequence:

>657_bases
ATGAGCAAAATTTCACTGGATGCACTGAATGTAAGAAATGCCTTGATTGAAAAAGGTATTGAGACACCCATGATTGATCC
AACACAGGCTAAAAATGAACGCCGAGAAAGCATTGCAAAACACATGCATGAAGTAATGAAGCTGATTGGATTAGATTTGC
GAGATGATAGTTTAGAAGAAACGCCAAATCGTTTAGCTAAAATGTTTATTGATGAGATTTTTAGTGGAATGGATTATGCG
AATTTTCCAAAGATGACAAAAATCAAAAATCAAATGAAAGTCAGCGAAATGGTGCAAGTGAATGACATCACGCTAACAAG
CACTTGTGAACATCATTTTGTGACGATTGATGGTAAAGTTTGTGTGGCGTACTATCCAAAAGATTGGGTGATTGGTCTTT
CTAAAATTAATCGAATTGTCTCGTTTTTTGCGCAACGTCCACAGGTTCAAGAACGCTTAACTGAGCAACTTTTGACAGCA
TTTCAAACCATTCTTGAAACCGATGATGTGGCGGTATATGTGAAAGCGACCCATTTCTGTGTGAAAGCGCGTGGTATTAG
AGATACAAATAGTTATACTGTCACATATGCTTATGGTGGCGTATTCTTAGAAGATAGAGATACTCGTAAAGAATTTTTAG
CCACAGTTCAAAAATAG

Upstream 100 bases:

>100_bases
ATATCTAAAGCCTAATTCATTCAAATTTGCAATTTTTCTCATTTTACCATCCTATTATCCTTTGCTAGAATAGCCGATTA
TTTTTTTAGGAAAGACTATT

Downstream 100 bases:

>100_bases
GAAATGATAAATTGTAATCCGCACGTAAAATGTGCGGATTTTTATTTCGGGAAATTAAATATGCTATGTGCCATTTATAA
AAGTAAAAAGAAGTTAGGAA

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: GTP cyclohydrolase I; GTP-CH-I [H]

Number of amino acids: Translated: 218; Mature: 217

Protein sequence:

>218_residues
MSKISLDALNVRNALIEKGIETPMIDPTQAKNERRESIAKHMHEVMKLIGLDLRDDSLEETPNRLAKMFIDEIFSGMDYA
NFPKMTKIKNQMKVSEMVQVNDITLTSTCEHHFVTIDGKVCVAYYPKDWVIGLSKINRIVSFFAQRPQVQERLTEQLLTA
FQTILETDDVAVYVKATHFCVKARGIRDTNSYTVTYAYGGVFLEDRDTRKEFLATVQK

Sequences:

>Translated_218_residues
MSKISLDALNVRNALIEKGIETPMIDPTQAKNERRESIAKHMHEVMKLIGLDLRDDSLEETPNRLAKMFIDEIFSGMDYA
NFPKMTKIKNQMKVSEMVQVNDITLTSTCEHHFVTIDGKVCVAYYPKDWVIGLSKINRIVSFFAQRPQVQERLTEQLLTA
FQTILETDDVAVYVKATHFCVKARGIRDTNSYTVTYAYGGVFLEDRDTRKEFLATVQK
>Mature_217_residues
SKISLDALNVRNALIEKGIETPMIDPTQAKNERRESIAKHMHEVMKLIGLDLRDDSLEETPNRLAKMFIDEIFSGMDYAN
FPKMTKIKNQMKVSEMVQVNDITLTSTCEHHFVTIDGKVCVAYYPKDWVIGLSKINRIVSFFAQRPQVQERLTEQLLTAF
QTILETDDVAVYVKATHFCVKARGIRDTNSYTVTYAYGGVFLEDRDTRKEFLATVQK

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family [H]

Homologues:

Organism=Homo sapiens, GI66932968, Length=122, Percent_Identity=49.1803278688525, Blast_Score=119, Evalue=3e-27,
Organism=Homo sapiens, GI4503949, Length=122, Percent_Identity=49.1803278688525, Blast_Score=119, Evalue=3e-27,
Organism=Homo sapiens, GI66932970, Length=82, Percent_Identity=50, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI66932972, Length=82, Percent_Identity=50, Blast_Score=81, Evalue=6e-16,
Organism=Escherichia coli, GI1788476, Length=219, Percent_Identity=63.9269406392694, Blast_Score=280, Evalue=5e-77,
Organism=Caenorhabditis elegans, GI17560486, Length=206, Percent_Identity=35.4368932038835, Blast_Score=122, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6321706, Length=188, Percent_Identity=38.2978723404255, Blast_Score=113, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24656796, Length=121, Percent_Identity=47.9338842975207, Blast_Score=107, Evalue=8e-24,
Organism=Drosophila melanogaster, GI24656782, Length=121, Percent_Identity=47.9338842975207, Blast_Score=107, Evalue=9e-24,
Organism=Drosophila melanogaster, GI24656791, Length=121, Percent_Identity=47.9338842975207, Blast_Score=106, Evalue=9e-24,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =3.5.4.16 [H]

Molecular weight: Translated: 25021; Mature: 24890

Theoretical pI: Translated: 7.05; Mature: 7.05

Prosite motif: PS00859 GTP_CYCLOHYDROL_1_1 ; PS00860 GTP_CYCLOHYDROL_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKISLDALNVRNALIEKGIETPMIDPTQAKNERRESIAKHMHEVMKLIGLDLRDDSLEE
CCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
TPNRLAKMFIDEIFSGMDYANFPKMTKIKNQMKVSEMVQVNDITLTSTCEHHFVTIDGKV
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHEECCEEEEECCCCEEEEECCEE
CVAYYPKDWVIGLSKINRIVSFFAQRPQVQERLTEQLLTAFQTILETDDVAVYVKATHFC
EEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEHHHH
VKARGIRDTNSYTVTYAYGGVFLEDRDTRKEFLATVQK
HHCCCCCCCCCEEEEEEECEEEECCCHHHHHHHHHHCC
>Mature Secondary Structure 
SKISLDALNVRNALIEKGIETPMIDPTQAKNERRESIAKHMHEVMKLIGLDLRDDSLEE
CCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
TPNRLAKMFIDEIFSGMDYANFPKMTKIKNQMKVSEMVQVNDITLTSTCEHHFVTIDGKV
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHEECCEEEEECCCCEEEEECCEE
CVAYYPKDWVIGLSKINRIVSFFAQRPQVQERLTEQLLTAFQTILETDDVAVYVKATHFC
EEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEHHHH
VKARGIRDTNSYTVTYAYGGVFLEDRDTRKEFLATVQK
HHCCCCCCCCCEEEEEEECEEEECCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA