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The map label for this gene is purN [H]

Identifier: 148826928

GI number: 148826928

Start: 174516

End: 175154

Strand: Reverse

Name: purN [H]

Synonym: CGSHiGG_01010

Alternate gene names: 148826928

Gene position: 175154-174516 (Counterclockwise)

Preceding gene: 148826929

Following gene: 148826924

Centisome position: 9.28

GC content: 40.53

Gene sequence:

>639_bases
ATGAAAAAAATTGCCGTCCTTATTTCTGGTCAAGGAACAAATTTACAAACTATTATTGATGCCTGCCATTTAGGCGATAT
TCCCGCCAAAATTGCTTGTGTGGTAAGTAACAAAGCTGATGCTTACGGCTTAGTTCGAGCCAAACAAGCACAGATTCCGC
AAGCGGTCTTTCTTCGCAAAAATTTTTCCAATAACCTCGAAATGGACGACGCCATTGGCGATTATTTGCAAAGCCTAGCC
GTAGATTTGATCGTCCTTGCTGGTTATATGAAAATTCTTACCCCAAAATTTACCCAACGTTTTGCAGGGAAAATTTTAAA
TATCCATCCTTCTCTTCTTCCTAAATACGCAGGCTTAAACACCTACCAACGTGCGATTGAAGCAGGCGACAATGAACACG
GCACAACCGTTCATTTTGTCAATGAAGAAGTAGATGGTGGTGCGATTGTCTTACAAGCAAAAGTGCCAATCTTCCCTGAA
GATAGCATCGAAGAAGTGGAGGCTAGAACCCGTGAGCAAGAATATCAAATTTATCCGCTTGTGATTAAATGGTTTACCGA
AGGGCGGTTAAGACTTAAAGATAATTTGGCATATTTAGATGGGAAAGCTCTGCCAAAATCTGGATATGCAAATGAATAA

Upstream 100 bases:

>100_bases
AATTGAACATCTTGGCGAAGGCGAAGCACAAGTTGAGATTCAATAATAAAATCTTAAATTTAAATTCAGCCCTTTTGGGC
TGATTTCTATAAGGAAGAAA

Downstream 100 bases:

>100_bases
CTTAGAATTTAGACAGACTATTTCTGTATTTTTTATTTAATTAAGATGTAAAAGTGCGGTGAAATTTTACCGCACTTTTA
AAATTAGTGATGATGGTATT

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MKKIAVLISGQGTNLQTIIDACHLGDIPAKIACVVSNKADAYGLVRAKQAQIPQAVFLRKNFSNNLEMDDAIGDYLQSLA
VDLIVLAGYMKILTPKFTQRFAGKILNIHPSLLPKYAGLNTYQRAIEAGDNEHGTTVHFVNEEVDGGAIVLQAKVPIFPE
DSIEEVEARTREQEYQIYPLVIKWFTEGRLRLKDNLAYLDGKALPKSGYANE

Sequences:

>Translated_212_residues
MKKIAVLISGQGTNLQTIIDACHLGDIPAKIACVVSNKADAYGLVRAKQAQIPQAVFLRKNFSNNLEMDDAIGDYLQSLA
VDLIVLAGYMKILTPKFTQRFAGKILNIHPSLLPKYAGLNTYQRAIEAGDNEHGTTVHFVNEEVDGGAIVLQAKVPIFPE
DSIEEVEARTREQEYQIYPLVIKWFTEGRLRLKDNLAYLDGKALPKSGYANE
>Mature_212_residues
MKKIAVLISGQGTNLQTIIDACHLGDIPAKIACVVSNKADAYGLVRAKQAQIPQAVFLRKNFSNNLEMDDAIGDYLQSLA
VDLIVLAGYMKILTPKFTQRFAGKILNIHPSLLPKYAGLNTYQRAIEAGDNEHGTTVHFVNEEVDGGAIVLQAKVPIFPE
DSIEEVEARTREQEYQIYPLVIKWFTEGRLRLKDNLAYLDGKALPKSGYANE

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=193, Percent_Identity=41.9689119170984, Blast_Score=167, Evalue=6e-42,
Organism=Homo sapiens, GI209869995, Length=193, Percent_Identity=41.9689119170984, Blast_Score=167, Evalue=6e-42,
Organism=Homo sapiens, GI209869993, Length=193, Percent_Identity=41.9689119170984, Blast_Score=167, Evalue=6e-42,
Organism=Escherichia coli, GI1788846, Length=207, Percent_Identity=52.6570048309179, Blast_Score=236, Evalue=7e-64,
Organism=Escherichia coli, GI1787483, Length=176, Percent_Identity=30.1136363636364, Blast_Score=92, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17567511, Length=177, Percent_Identity=37.8531073446328, Blast_Score=125, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6320616, Length=198, Percent_Identity=33.3333333333333, Blast_Score=83, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24582400, Length=189, Percent_Identity=44.4444444444444, Blast_Score=166, Evalue=1e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 23475; Mature: 23475

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIAVLISGQGTNLQTIIDACHLGDIPAKIACVVSNKADAYGLVRAKQAQIPQAVFLRK
CCEEEEEEECCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCHHHEEHHHHCCCHHHHHHH
NFSNNLEMDDAIGDYLQSLAVDLIVLAGYMKILTPKFTQRFAGKILNIHPSLLPKYAGLN
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHEEECCHHHHHHHCCCH
TYQRAIEAGDNEHGTTVHFVNEEVDGGAIVLQAKVPIFPEDSIEEVEARTREQEYQIYPL
HHHHHHHCCCCCCCCEEEEECCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCEEEEEE
VIKWFTEGRLRLKDNLAYLDGKALPKSGYANE
EEEEECCCCEEEECCEEEECCCCCCCCCCCCC
>Mature Secondary Structure
MKKIAVLISGQGTNLQTIIDACHLGDIPAKIACVVSNKADAYGLVRAKQAQIPQAVFLRK
CCEEEEEEECCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCHHHEEHHHHCCCHHHHHHH
NFSNNLEMDDAIGDYLQSLAVDLIVLAGYMKILTPKFTQRFAGKILNIHPSLLPKYAGLN
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHEEECCHHHHHHHCCCH
TYQRAIEAGDNEHGTTVHFVNEEVDGGAIVLQAKVPIFPEDSIEEVEARTREQEYQIYPL
HHHHHHHCCCCCCCCEEEEECCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCEEEEEE
VIKWFTEGRLRLKDNLAYLDGKALPKSGYANE
EEEEECCCCEEEECCEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]