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The map label for this gene is glgX [H]

Identifier: 148826829

GI number: 148826829

Start: 78663

End: 80642

Strand: Direct

Name: glgX [H]

Synonym: CGSHiGG_00445

Alternate gene names: 148826829

Gene position: 78663-80642 (Clockwise)

Preceding gene: 148826828

Following gene: 148826830

Centisome position: 4.17

GC content: 41.57

Gene sequence:

>1980_bases
ATGTTCAAAATCTACAACAACGGCAATCCTATTCCAATGGGATACTCACAAGCGGTCGAAAACAACGTGCAAATTACCAA
TTTCGCCCTGTTTTCCGTTGCGGCAATAGGCGTAGAACTTTGCCTTTTTGACGAACAAAATCAAGAAACACGCTTGCCGA
TGGTGCGTACTGAAAATGTGTGGCATTTGGCGGTAACTGGCGTGAAAACTGGTACGGAATATGGATTTCGTATTCACGGA
GAATTTGCCAATCCGCAGAAATTGATACTCGATCCCTATGCGAAAGCCGTGAATGGTAAACCTGATTTAAGTAGCGAAGA
AAGTCGTTCTTGGTTCTTGCTTTCGGATAATCGCGACAACGCGCATCTTGCCCCAAGAGCGGTTGTAATTTCAGAGGAAT
TTGATTGGGAAAATGACACTTCACCCAATACGCCTTGGGCTGAAACCATTGTGTATGAATTACATGTTAAAGGGTTTAGC
CAGTTAAACGAGAAAATCCCTGCGGCGTTGCGTGGTACTTATACTGGATTAGCACATCCTGTGAATTTGGCGTATTTGAA
AGAATTAGGCGTAACTGCGGTGGAGTTGCTGCCTGTAAATTTCCATATAAATGAACCGCACTTACAGGCACGAGGCTTAC
AAAATTATTGGGGATACAATCCCTTAGCGATGTTTGCGGTAGAACCTAAATATGCGGCAACAAATAATCCATTGGCTGAA
TTTAAAACGATGGTAAAGGCATTTCACAATGCAGGTATTGAAGTTATCTTAGACGTGGTGTTTAACCATTCCGCAGAATC
AGAGCAAACTTACCCAACATTCTGCCAGCGTGGCATTGATAATAAAACTTACTATTGGCGCAACGATCAAGGGCGTTATA
TCAATTGGACAGGCTGTGGCAATATGCTCAATTTATCCTCTGATGTAGGGCGAAAGTGGGTGGTGGACTGCCTGCGTTAT
TGGGTGGAGCAATGCCACATTGATGGATTCCGTTTTGATTTAGCCACTGTTTTAGGGCGTGATACACCTGATTTCAACTC
GTCAGCTCAGCTTTTTACTGACATAAAAAATGAGCCAAGTTTACAAAATATTAAATTGATTGCCGAGCCGTGGGATATTG
GACATTACGGTTATCAAGTCGGCAATTTCCCAAGTTATTTTGCCGAATGGAACGACCGATTCCGTGATGATCTCTGCCGT
TTCTGGCTATGGAAAAGTGGCGAAATTGGTGCATTTGCAGAACGTTTTGCAGGTTCTAGCGATTTATTTAAGAAAAATGA
CCGCTTGCCACATACTACACTTAATTTTATTACTGCTCACGATGGTTTTACGCTTAAAGATTTAGTGAGTTATAACCAAA
AACATAACGAAACGAATGGCGAAGAAAATCGTGATGGTCGCAATGAAAATTACAGTTATAACCATGGAGTAGAAGGTTCT
ACAGAATCCCTTTCTGAACCGCAAAAAAGTGCGGTGGAAAATAACCGCACTTTTGCACAAAGCGGATTATTAATGAGTTT
ATTGCTGGCGAATGGTACACCTATGTTGTTAGCTGGCGATGAGTTCGGCAACACTCAATATGGTAACAATAATGCTTATT
GCCAAGATAACGAGATTACTTGGCTGAAATGGGCAAATTTTAACGAAGAATTATTTGAACTGACCAAACAAACGATTGCA
CTTCGTAAGCAAATTGGCAGTTTAAATAAAGATCAATGGTGGTCGGATGAGAATGTGCAATGGCTTAACATTGCAGGCGA
ACCGATGACAGTTGAAGATTGGCAAAATCAACAAACCAAAGCATTACAGGTGGTTTTAGACAATCGTTGGCTTTTGTTGA
TTAATGCCAAAGCCGAAGGTCAAATGTTTCACTTGCCTAATGGGAAATGGAAGCCCCAAATCGGCACACATAATGTAACC
CTTGAAGCACAACAAGCCGAACTTAGTTCAATGGGCTTTTGTATGCTGAATGACGAATAA

Upstream 100 bases:

>100_bases
AATCCGAATAGATAAATTGTAGGGGCGAATTACATTCGCCCTGATAAACCACCGATGTTTTTGGGCAAATGTAATTTGCC
CCTACAAAAGCGATAAAAAT

Downstream 100 bases:

>100_bases
CAATAAGACTATGGAGATCCTTATGAAATCTGGCGACCTTAACAAATATGATTTAGTTAAAAACACTTTGGTGCTTGTAC
TTGCAGGTGGACGTGGTTCA

Product: glycogen branching enzyme

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 659; Mature: 659

Protein sequence:

>659_residues
MFKIYNNGNPIPMGYSQAVENNVQITNFALFSVAAIGVELCLFDEQNQETRLPMVRTENVWHLAVTGVKTGTEYGFRIHG
EFANPQKLILDPYAKAVNGKPDLSSEESRSWFLLSDNRDNAHLAPRAVVISEEFDWENDTSPNTPWAETIVYELHVKGFS
QLNEKIPAALRGTYTGLAHPVNLAYLKELGVTAVELLPVNFHINEPHLQARGLQNYWGYNPLAMFAVEPKYAATNNPLAE
FKTMVKAFHNAGIEVILDVVFNHSAESEQTYPTFCQRGIDNKTYYWRNDQGRYINWTGCGNMLNLSSDVGRKWVVDCLRY
WVEQCHIDGFRFDLATVLGRDTPDFNSSAQLFTDIKNEPSLQNIKLIAEPWDIGHYGYQVGNFPSYFAEWNDRFRDDLCR
FWLWKSGEIGAFAERFAGSSDLFKKNDRLPHTTLNFITAHDGFTLKDLVSYNQKHNETNGEENRDGRNENYSYNHGVEGS
TESLSEPQKSAVENNRTFAQSGLLMSLLLANGTPMLLAGDEFGNTQYGNNNAYCQDNEITWLKWANFNEELFELTKQTIA
LRKQIGSLNKDQWWSDENVQWLNIAGEPMTVEDWQNQQTKALQVVLDNRWLLLINAKAEGQMFHLPNGKWKPQIGTHNVT
LEAQQAELSSMGFCMLNDE

Sequences:

>Translated_659_residues
MFKIYNNGNPIPMGYSQAVENNVQITNFALFSVAAIGVELCLFDEQNQETRLPMVRTENVWHLAVTGVKTGTEYGFRIHG
EFANPQKLILDPYAKAVNGKPDLSSEESRSWFLLSDNRDNAHLAPRAVVISEEFDWENDTSPNTPWAETIVYELHVKGFS
QLNEKIPAALRGTYTGLAHPVNLAYLKELGVTAVELLPVNFHINEPHLQARGLQNYWGYNPLAMFAVEPKYAATNNPLAE
FKTMVKAFHNAGIEVILDVVFNHSAESEQTYPTFCQRGIDNKTYYWRNDQGRYINWTGCGNMLNLSSDVGRKWVVDCLRY
WVEQCHIDGFRFDLATVLGRDTPDFNSSAQLFTDIKNEPSLQNIKLIAEPWDIGHYGYQVGNFPSYFAEWNDRFRDDLCR
FWLWKSGEIGAFAERFAGSSDLFKKNDRLPHTTLNFITAHDGFTLKDLVSYNQKHNETNGEENRDGRNENYSYNHGVEGS
TESLSEPQKSAVENNRTFAQSGLLMSLLLANGTPMLLAGDEFGNTQYGNNNAYCQDNEITWLKWANFNEELFELTKQTIA
LRKQIGSLNKDQWWSDENVQWLNIAGEPMTVEDWQNQQTKALQVVLDNRWLLLINAKAEGQMFHLPNGKWKPQIGTHNVT
LEAQQAELSSMGFCMLNDE
>Mature_659_residues
MFKIYNNGNPIPMGYSQAVENNVQITNFALFSVAAIGVELCLFDEQNQETRLPMVRTENVWHLAVTGVKTGTEYGFRIHG
EFANPQKLILDPYAKAVNGKPDLSSEESRSWFLLSDNRDNAHLAPRAVVISEEFDWENDTSPNTPWAETIVYELHVKGFS
QLNEKIPAALRGTYTGLAHPVNLAYLKELGVTAVELLPVNFHINEPHLQARGLQNYWGYNPLAMFAVEPKYAATNNPLAE
FKTMVKAFHNAGIEVILDVVFNHSAESEQTYPTFCQRGIDNKTYYWRNDQGRYINWTGCGNMLNLSSDVGRKWVVDCLRY
WVEQCHIDGFRFDLATVLGRDTPDFNSSAQLFTDIKNEPSLQNIKLIAEPWDIGHYGYQVGNFPSYFAEWNDRFRDDLCR
FWLWKSGEIGAFAERFAGSSDLFKKNDRLPHTTLNFITAHDGFTLKDLVSYNQKHNETNGEENRDGRNENYSYNHGVEGS
TESLSEPQKSAVENNRTFAQSGLLMSLLLANGTPMLLAGDEFGNTQYGNNNAYCQDNEITWLKWANFNEELFELTKQTIA
LRKQIGSLNKDQWWSDENVQWLNIAGEPMTVEDWQNQQTKALQVVLDNRWLLLINAKAEGQMFHLPNGKWKPQIGTHNVT
LEAQQAELSSMGFCMLNDE

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=667, Percent_Identity=50.2248875562219, Blast_Score=643, Evalue=0.0,
Organism=Escherichia coli, GI1789839, Length=343, Percent_Identity=24.7813411078717, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR011837
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 75179; Mature: 75179

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKIYNNGNPIPMGYSQAVENNVQITNFALFSVAAIGVELCLFDEQNQETRLPMVRTENV
CEEEECCCCCCCCCHHHHHCCCCEEEEHHHHHHHHHCEEEEEECCCCCCCCCCEEEECCE
WHLAVTGVKTGTEYGFRIHGEFANPQKLILDPYAKAVNGKPDLSSEESRSWFLLSDNRDN
EEEEEEEEECCCCCCEEEECCCCCCCEEEECHHHHHCCCCCCCCCCCCCEEEEEECCCCC
AHLAPRAVVISEEFDWENDTSPNTPWAETIVYELHVKGFSQLNEKIPAALRGTYTGLAHP
CCCCCEEEEEECCCCCCCCCCCCCCHHHEEEEEEECCCHHHHHHHCCHHHHCCCCCCCCC
VNLAYLKELGVTAVELLPVNFHINEPHLQARGLQNYWGYNPLAMFAVEPKYAATNNPLAE
CHHHHHHHCCCEEEEEEEEEEEECCCCHHHCCCHHHCCCCCEEEEEECCCCCCCCCCHHH
FKTMVKAFHNAGIEVILDVVFNHSAESEQTYPTFCQRGIDNKTYYWRNDQGRYINWTGCG
HHHHHHHHHHCCHHEEHHHHHCCCCCCCCCCHHHHHCCCCCCEEEEECCCCCEEEEECCC
NMLNLSSDVGRKWVVDCLRYWVEQCHIDGFRFDLATVLGRDTPDFNSSAQLFTDIKNEPS
CEEECCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCHHHHHHCCCCCC
LQNIKLIAEPWDIGHYGYQVGNFPSYFAEWNDRFRDDLCRFWLWKSGEIGAFAERFAGSS
CCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCC
DLFKKNDRLPHTTLNFITAHDGFTLKDLVSYNQKHNETNGEENRDGRNENYSYNHGVEGS
HHHHCCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
TESLSEPQKSAVENNRTFAQSGLLMSLLLANGTPMLLAGDEFGNTQYGNNNAYCQDNEIT
HHHHCCHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCEEECCCEE
WLKWANFNEELFELTKQTIALRKQIGSLNKDQWWSDENVQWLNIAGEPMTVEDWQNQQTK
EEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCHHCCCCCHHE
ALQVVLDNRWLLLINAKAEGQMFHLPNGKWKPQIGTHNVTLEAQQAELSSMGFCMLNDE
EEEEEECCCEEEEEECCCCCEEEECCCCCCCCCCCCCEEEEEHHHHHHHHCCEEEECCC
>Mature Secondary Structure
MFKIYNNGNPIPMGYSQAVENNVQITNFALFSVAAIGVELCLFDEQNQETRLPMVRTENV
CEEEECCCCCCCCCHHHHHCCCCEEEEHHHHHHHHHCEEEEEECCCCCCCCCCEEEECCE
WHLAVTGVKTGTEYGFRIHGEFANPQKLILDPYAKAVNGKPDLSSEESRSWFLLSDNRDN
EEEEEEEEECCCCCCEEEECCCCCCCEEEECHHHHHCCCCCCCCCCCCCEEEEEECCCCC
AHLAPRAVVISEEFDWENDTSPNTPWAETIVYELHVKGFSQLNEKIPAALRGTYTGLAHP
CCCCCEEEEEECCCCCCCCCCCCCCHHHEEEEEEECCCHHHHHHHCCHHHHCCCCCCCCC
VNLAYLKELGVTAVELLPVNFHINEPHLQARGLQNYWGYNPLAMFAVEPKYAATNNPLAE
CHHHHHHHCCCEEEEEEEEEEEECCCCHHHCCCHHHCCCCCEEEEEECCCCCCCCCCHHH
FKTMVKAFHNAGIEVILDVVFNHSAESEQTYPTFCQRGIDNKTYYWRNDQGRYINWTGCG
HHHHHHHHHHCCHHEEHHHHHCCCCCCCCCCHHHHHCCCCCCEEEEECCCCCEEEEECCC
NMLNLSSDVGRKWVVDCLRYWVEQCHIDGFRFDLATVLGRDTPDFNSSAQLFTDIKNEPS
CEEECCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCHHHHHHCCCCCC
LQNIKLIAEPWDIGHYGYQVGNFPSYFAEWNDRFRDDLCRFWLWKSGEIGAFAERFAGSS
CCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCC
DLFKKNDRLPHTTLNFITAHDGFTLKDLVSYNQKHNETNGEENRDGRNENYSYNHGVEGS
HHHHCCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
TESLSEPQKSAVENNRTFAQSGLLMSLLLANGTPMLLAGDEFGNTQYGNNNAYCQDNEIT
HHHHCCHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCEEECCCEE
WLKWANFNEELFELTKQTIALRKQIGSLNKDQWWSDENVQWLNIAGEPMTVEDWQNQQTK
EEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCHHCCCCCHHE
ALQVVLDNRWLLLINAKAEGQMFHLPNGKWKPQIGTHNVTLEAQQAELSSMGFCMLNDE
EEEEEECCCEEEEEECCCCCEEEECCCCCCCCCCCCCEEEEEHHHHHHHHCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Glycosidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]