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The map label for this gene is glnS [H]

Identifier: 148826826

GI number: 148826826

Start: 72008

End: 73672

Strand: Direct

Name: glnS [H]

Synonym: CGSHiGG_00420

Alternate gene names: 148826826

Gene position: 72008-73672 (Clockwise)

Preceding gene: 148826823

Following gene: 148826827

Centisome position: 3.82

GC content: 39.28

Gene sequence:

>1665_bases
ATGACAGAGACATCTTTGGGCGCAGAAAATACTCGCACTCACAATTTTATTACCCAAATTATTGATGAAGATTTAGCTTC
GGGTAAACATAAAAGCGTTCATACCCGTTTTCCGCCAGAGCCGAATGGCTATTTGCATATTGGTCACGCAAAGTCTATTT
GCTTGAATTTTGGCTTGGCAAAAGAATACCAAGGTTTATGTAACCTACGTTTTGACGATACCAATCCAGTAAAAGAAGAC
GTGGAATATGTAGATTCTATCAAAGCTGACGTGGAATGGTTAGGTTTTAAATGGGAGGGCGAACCGCGTTATGCATCTGA
TTATTTTGATGCGCTTTATAGCTATGCCATTGAATTAATTAAAAAAGGTTTGGCTTATGTTGATGAACTTTCGCCAGATG
AAATGCGTGAATATCGTGGCACATTAACTGAAGCAGGTAAAAACAGCCCATATCGTGACCGCACTATTGAAGAAAACTTA
GCTTTATTTGAAAAAATGAAAAATGGCGAATTTGCAGAGGGTAAAGCAAGTTTACGTGCAAAAATTGATATGGCATCGCC
ATTTATGGTCATGCGTGATCCTGTAATTTATCGCATTAAATTTGCCAGCCATCATCAAACAGGCGATAAATGGTGCATTT
ACCCAATGTACGATTTCACTCACTGTATCTCTGATGCCATTGAACGTATTACTCACTCGATCTGTACATTAGAATTCCAA
GATAACCGTCGTTTATATGATTGGGTGTTGGAAAATATCAGTATTGAACGTCCATTACCGCATCAATATGAATTCTCACG
TTTAAATTTAGAAGGCACATTGACGTCTAAACGTAAGTTATTGAAATTAGTAAACGAGGGCATTGTAGATGGTTGGAATG
ATCCTCGTATGCCAACCATTTCAGGTTTACGCCGTCGTGGTTATACACCTGCTTCGTTACGTGAATTTTGTCGCCGTATT
GGTGTGACTAAACAAGATAACGTGGTAGAATATTCTGCACTTGAAGCCTGCATTCGTGAGGATTTGAACGAAAACGCACC
ACGCGCAATGGCAGTGATTGATCCTGTTCGTGTGGTAATTGAAAACTTTGAAGGCGAGGAAGTTTTAACTGCCCCAAATC
ATCCAAATCGTCCAGAATTAGGCGAGCGTCAATTGCCGTTTACGAAAGAGCTTTATATTGATCGAGCTGACTTCCGTGAG
GAAGCGAACAAACAATATAAACGTTTAGTATTAGGCAAAGAAGTACGTTTACGTAATGCTTACGTGATTAAGGCTGAACG
TGTCGAAAAAGATGCAAGTGGCGAAATTACCACGATTTTCTGTACTTATGATCCTGAAACCTTAGGTAAAAATCCATCAG
ATGGGCGTAAAGTGAAAGGTGTCATTCATTGGGTTTCTGCTACGCATAATCATCCAACTGAATTCCGTTTGTATGATCGT
TTATTCACTGTGCCAAATCCAGGTGCTGAAGATGATATTGAAAGCGTGTTAAATCCAAATTCTTTAGTGATAAAACAAGG
TTTTGTAGAACAAAGCTTGGCTGCTGCAGAAGCAGAGAAAGGTTATCAATTTGAACGTGAGGGTTATTTCTGTGCAGATA
GTAAAGATAGCCGTCCTGAACATTTGGTGTTTAATTTAACGGTAAGTCTAAAAGAGGGCTTCTAA

Upstream 100 bases:

>100_bases
AATCCAGTATTTCTATGGCAAGAACAGGAGATGAACCTTTATTTTCCCTTAGATTTGTATAGAATAGCCAAATATTTTTT
TATTTTAACGAGAGAAAATG

Downstream 100 bases:

>100_bases
TTTTAATCAACCAAGTGCGGTCAAAATAAACGTAATTTTGACCGCACTTTTTATTTGTATCAACTATGCAATCCAATATG
CAACTTGAACCCAATTTCTG

Product: glutaminyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamine--tRNA ligase; GlnRS [H]

Number of amino acids: Translated: 554; Mature: 553

Protein sequence:

>554_residues
MTETSLGAENTRTHNFITQIIDEDLASGKHKSVHTRFPPEPNGYLHIGHAKSICLNFGLAKEYQGLCNLRFDDTNPVKED
VEYVDSIKADVEWLGFKWEGEPRYASDYFDALYSYAIELIKKGLAYVDELSPDEMREYRGTLTEAGKNSPYRDRTIEENL
ALFEKMKNGEFAEGKASLRAKIDMASPFMVMRDPVIYRIKFASHHQTGDKWCIYPMYDFTHCISDAIERITHSICTLEFQ
DNRRLYDWVLENISIERPLPHQYEFSRLNLEGTLTSKRKLLKLVNEGIVDGWNDPRMPTISGLRRRGYTPASLREFCRRI
GVTKQDNVVEYSALEACIREDLNENAPRAMAVIDPVRVVIENFEGEEVLTAPNHPNRPELGERQLPFTKELYIDRADFRE
EANKQYKRLVLGKEVRLRNAYVIKAERVEKDASGEITTIFCTYDPETLGKNPSDGRKVKGVIHWVSATHNHPTEFRLYDR
LFTVPNPGAEDDIESVLNPNSLVIKQGFVEQSLAAAEAEKGYQFEREGYFCADSKDSRPEHLVFNLTVSLKEGF

Sequences:

>Translated_554_residues
MTETSLGAENTRTHNFITQIIDEDLASGKHKSVHTRFPPEPNGYLHIGHAKSICLNFGLAKEYQGLCNLRFDDTNPVKED
VEYVDSIKADVEWLGFKWEGEPRYASDYFDALYSYAIELIKKGLAYVDELSPDEMREYRGTLTEAGKNSPYRDRTIEENL
ALFEKMKNGEFAEGKASLRAKIDMASPFMVMRDPVIYRIKFASHHQTGDKWCIYPMYDFTHCISDAIERITHSICTLEFQ
DNRRLYDWVLENISIERPLPHQYEFSRLNLEGTLTSKRKLLKLVNEGIVDGWNDPRMPTISGLRRRGYTPASLREFCRRI
GVTKQDNVVEYSALEACIREDLNENAPRAMAVIDPVRVVIENFEGEEVLTAPNHPNRPELGERQLPFTKELYIDRADFRE
EANKQYKRLVLGKEVRLRNAYVIKAERVEKDASGEITTIFCTYDPETLGKNPSDGRKVKGVIHWVSATHNHPTEFRLYDR
LFTVPNPGAEDDIESVLNPNSLVIKQGFVEQSLAAAEAEKGYQFEREGYFCADSKDSRPEHLVFNLTVSLKEGF
>Mature_553_residues
TETSLGAENTRTHNFITQIIDEDLASGKHKSVHTRFPPEPNGYLHIGHAKSICLNFGLAKEYQGLCNLRFDDTNPVKEDV
EYVDSIKADVEWLGFKWEGEPRYASDYFDALYSYAIELIKKGLAYVDELSPDEMREYRGTLTEAGKNSPYRDRTIEENLA
LFEKMKNGEFAEGKASLRAKIDMASPFMVMRDPVIYRIKFASHHQTGDKWCIYPMYDFTHCISDAIERITHSICTLEFQD
NRRLYDWVLENISIERPLPHQYEFSRLNLEGTLTSKRKLLKLVNEGIVDGWNDPRMPTISGLRRRGYTPASLREFCRRIG
VTKQDNVVEYSALEACIREDLNENAPRAMAVIDPVRVVIENFEGEEVLTAPNHPNRPELGERQLPFTKELYIDRADFREE
ANKQYKRLVLGKEVRLRNAYVIKAERVEKDASGEITTIFCTYDPETLGKNPSDGRKVKGVIHWVSATHNHPTEFRLYDRL
FTVPNPGAEDDIESVLNPNSLVIKQGFVEQSLAAAEAEKGYQFEREGYFCADSKDSRPEHLVFNLTVSLKEGF

Specific function: Unknown

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI4826960, Length=530, Percent_Identity=47.5471698113208, Blast_Score=450, Evalue=1e-126,
Organism=Homo sapiens, GI62241042, Length=516, Percent_Identity=34.3023255813954, Blast_Score=281, Evalue=8e-76,
Organism=Escherichia coli, GI1786895, Length=546, Percent_Identity=77.1062271062271, Blast_Score=891, Evalue=0.0,
Organism=Escherichia coli, GI1788743, Length=303, Percent_Identity=23.1023102310231, Blast_Score=69, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17543332, Length=532, Percent_Identity=41.9172932330827, Blast_Score=409, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI17511007, Length=521, Percent_Identity=32.0537428023033, Blast_Score=248, Evalue=5e-66,
Organism=Saccharomyces cerevisiae, GI6324742, Length=563, Percent_Identity=41.740674955595, Blast_Score=394, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6321192, Length=523, Percent_Identity=35.755258126195, Blast_Score=310, Evalue=3e-85,
Organism=Drosophila melanogaster, GI17864488, Length=542, Percent_Identity=42.9889298892989, Blast_Score=427, Evalue=1e-119,
Organism=Drosophila melanogaster, GI24649466, Length=519, Percent_Identity=34.6820809248555, Blast_Score=277, Evalue=1e-74,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR004514
- InterPro:   IPR022861
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR020059
- InterPro:   IPR020056
- InterPro:   IPR011035
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c; PF03950 tRNA-synt_1c_C [H]

EC number: =6.1.1.18 [H]

Molecular weight: Translated: 63682; Mature: 63551

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTETSLGAENTRTHNFITQIIDEDLASGKHKSVHTRFPPEPNGYLHIGHAKSICLNFGLA
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCHHHHHHHCCCH
KEYQGLCNLRFDDTNPVKEDVEYVDSIKADVEWLGFKWEGEPRYASDYFDALYSYAIELI
HHHHCCEEEEECCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHH
KKGLAYVDELSPDEMREYRGTLTEAGKNSPYRDRTIEENLALFEKMKNGEFAEGKASLRA
HHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEE
KIDMASPFMVMRDPVIYRIKFASHHQTGDKWCIYPMYDFTHCISDAIERITHSICTLEFQ
EEECCCCEEEECCCEEEEEEECCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHEEEEEC
DNRRLYDWVLENISIERPLPHQYEFSRLNLEGTLTSKRKLLKLVNEGIVDGWNDPRMPTI
CCCHHHHHHHHCCCCCCCCCCCCCCEEEEEEECHHHHHHHHHHHHHHCCCCCCCCCCCHH
SGLRRRGYTPASLREFCRRIGVTKQDNVVEYSALEACIREDLNENAPRAMAVIDPVRVVI
HHHHHCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHH
ENFEGEEVLTAPNHPNRPELGERQLPFTKELYIDRADFREEANKQYKRLVLGKEVRLRNA
HCCCCCEEEECCCCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHCCCEEECCE
YVIKAERVEKDASGEITTIFCTYDPETLGKNPSDGRKVKGVIHWVSATHNHPTEFRLYDR
EEEEHHHHCCCCCCCEEEEEEECCHHHCCCCCCCCCHHHHEEEEEECCCCCCCCEEEEHH
LFTVPNPGAEDDIESVLNPNSLVIKQGFVEQSLAAAEAEKGYQFEREGYFCADSKDSRPE
EEECCCCCCHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHCCEECCCCEEECCCCCCCCC
HLVFNLTVSLKEGF
EEEEEEEEEEECCC
>Mature Secondary Structure 
TETSLGAENTRTHNFITQIIDEDLASGKHKSVHTRFPPEPNGYLHIGHAKSICLNFGLA
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCHHHHHHHCCCH
KEYQGLCNLRFDDTNPVKEDVEYVDSIKADVEWLGFKWEGEPRYASDYFDALYSYAIELI
HHHHCCEEEEECCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHH
KKGLAYVDELSPDEMREYRGTLTEAGKNSPYRDRTIEENLALFEKMKNGEFAEGKASLRA
HHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEE
KIDMASPFMVMRDPVIYRIKFASHHQTGDKWCIYPMYDFTHCISDAIERITHSICTLEFQ
EEECCCCEEEECCCEEEEEEECCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHEEEEEC
DNRRLYDWVLENISIERPLPHQYEFSRLNLEGTLTSKRKLLKLVNEGIVDGWNDPRMPTI
CCCHHHHHHHHCCCCCCCCCCCCCCEEEEEEECHHHHHHHHHHHHHHCCCCCCCCCCCHH
SGLRRRGYTPASLREFCRRIGVTKQDNVVEYSALEACIREDLNENAPRAMAVIDPVRVVI
HHHHHCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHH
ENFEGEEVLTAPNHPNRPELGERQLPFTKELYIDRADFREEANKQYKRLVLGKEVRLRNA
HCCCCCEEEECCCCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHCCCEEECCE
YVIKAERVEKDASGEITTIFCTYDPETLGKNPSDGRKVKGVIHWVSATHNHPTEFRLYDR
EEEEHHHHCCCCCCCEEEEEEECCHHHCCCCCCCCCHHHHEEEEEECCCCCCCCEEEEHH
LFTVPNPGAEDDIESVLNPNSLVIKQGFVEQSLAAAEAEKGYQFEREGYFCADSKDSRPE
EEECCCCCCHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHCCEECCCCEEECCCCCCCCC
HLVFNLTVSLKEGF
EEEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA