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The map label for this gene is yaaJ [C]

Identifier: 148826472

GI number: 148826472

Start: 1512359

End: 1513729

Strand: Direct

Name: yaaJ [C]

Synonym: CGSHiEE_07615

Alternate gene names: 148826472

Gene position: 1512359-1513729 (Clockwise)

Preceding gene: 148826471

Following gene: 148826475

Centisome position: 83.42

GC content: 40.34

Gene sequence:

>1371_bases
ATGACAATTGAGTCAATTCTTTCAGCTATTGATAGCTTTATTTGGGGCGCACCTCTACTCATACTCTTATCGGGCACGGG
GCTCTATTTAACGTTACGCTTAGGCTTTATTCAAATTCGCTATTTACCACGTGCACTAGGTTATTTATTTAAAAAAGATA
AAGGTGGAAAAGGGGACGTTTCCTCTTTTGCCGCACTTTGTACTGCATTAGCAGCAACAATTGGAACGGGTAATATTGTG
GGCGTGGCAACTGCGGTACAAGCAGGTGGGCCAGGGGCTATTTTTTGGATGTGGCTGGTTGCTTTGTTAGGAATGGCAAC
AAAATATGCTGAATGTTTACTCGCGGTTAAATACCGTGTTTGCGATAAAAATGGCTTTATGGCTGGCGGCCCGATGTATT
ACATTGAACGTGGACTAGGTATCAAATGGTTAGCAAAATTATTTGCATTATTTGGTGTGATGGTGGCCTTTTTCGGGATC
GGCACATTCCCACAGGTCAATGCGATTACCCATGCAATGCAAGATACTTTTAATATTCCAGTTTTAGTTACTGCAATTAT
TGTGACGCTTTTAGTCGGCTTAATTATTCTTGGCGGTGTAAAACGTATTGCAACCGCATCATCGGTAATTGTGCCGTTTA
TGGCGATTTTATACGTCACGACTTCACTGGTTATTATCCTTTTAAATATAGAAAAAGTACTAGATGCGATTTTATTGATT
ATTGATAGTGCTTTTGATCCGCAAGCTGCGCTAGGTGGGGCTGTTGGCTTTACCGTAATGAAGGCTATTCAATCAGGCGT
TGCACGCGGCATTTTCTCCAATGAATCAGGTTTAGGAAGCGCACCAATTGCAGCTGCAGCGGCACAAACTCGTGAACCTG
TGCGACAAGGCTTAATTTCCATGACAGGTACATTCTTAGATACCATCATCGTTTGTACAATGACAGGTATTGTATTGGTA
TTAACGGGAGCGTGGAACAATCCTGAATTAGCGGGTGCTACTGTCACAAACTATGCCTTTGCACAAGGTTTAGGCACATC
AATTGGTGCAACGATTGTAACAGTCGGTTTATTATTTTTTGCATTCACGACTATTTTAGGTTGGTGTTACTACGGGGAAC
GTTGCTTTGTCTATCTCGTCGGTATTCGTGGCGTAAAACTATATCGTCTAGCTTATATTATGTTGGTTGGTTTAGGGGCA
TTCTTACACTTAAATTTAATATGGATTATCGCCGATATTGTAAATGGTTTGATGGCTTTCCCAAATCTTATCGCGCTTAT
TGGATTGCGAAAAGTGATCATTGAAGAAACAAAAGATTATTTTCAACGTTTAAAAATTAATCATTATGATCAAGATGAAG
TGATAAAATAG

Upstream 100 bases:

>100_bases
AGTGTGGAAATTATCTATCTTTTTACCACACTTTCTTTTCCATTGTATCTTTTCGTCTTCTTGTCGTGTTTATTTTTGAT
TTAAACGACGAGGAATCACA

Downstream 100 bases:

>100_bases
TGAAATAAAAGGTGCTGTTCAGCAAATTCTGTGAAACAACACCTTTATTTTTCTATTTTTAATCAGCACTATAATACAAT
TTTCCGATTTCAATTTTTTG

Product: putative Na+/alanine symporter

Products: Na (I) [Cytoplasm]; L-alanine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 456; Mature: 455

Protein sequence:

>456_residues
MTIESILSAIDSFIWGAPLLILLSGTGLYLTLRLGFIQIRYLPRALGYLFKKDKGGKGDVSSFAALCTALAATIGTGNIV
GVATAVQAGGPGAIFWMWLVALLGMATKYAECLLAVKYRVCDKNGFMAGGPMYYIERGLGIKWLAKLFALFGVMVAFFGI
GTFPQVNAITHAMQDTFNIPVLVTAIIVTLLVGLIILGGVKRIATASSVIVPFMAILYVTTSLVIILLNIEKVLDAILLI
IDSAFDPQAALGGAVGFTVMKAIQSGVARGIFSNESGLGSAPIAAAAAQTREPVRQGLISMTGTFLDTIIVCTMTGIVLV
LTGAWNNPELAGATVTNYAFAQGLGTSIGATIVTVGLLFFAFTTILGWCYYGERCFVYLVGIRGVKLYRLAYIMLVGLGA
FLHLNLIWIIADIVNGLMAFPNLIALIGLRKVIIEETKDYFQRLKINHYDQDEVIK

Sequences:

>Translated_456_residues
MTIESILSAIDSFIWGAPLLILLSGTGLYLTLRLGFIQIRYLPRALGYLFKKDKGGKGDVSSFAALCTALAATIGTGNIV
GVATAVQAGGPGAIFWMWLVALLGMATKYAECLLAVKYRVCDKNGFMAGGPMYYIERGLGIKWLAKLFALFGVMVAFFGI
GTFPQVNAITHAMQDTFNIPVLVTAIIVTLLVGLIILGGVKRIATASSVIVPFMAILYVTTSLVIILLNIEKVLDAILLI
IDSAFDPQAALGGAVGFTVMKAIQSGVARGIFSNESGLGSAPIAAAAAQTREPVRQGLISMTGTFLDTIIVCTMTGIVLV
LTGAWNNPELAGATVTNYAFAQGLGTSIGATIVTVGLLFFAFTTILGWCYYGERCFVYLVGIRGVKLYRLAYIMLVGLGA
FLHLNLIWIIADIVNGLMAFPNLIALIGLRKVIIEETKDYFQRLKINHYDQDEVIK
>Mature_455_residues
TIESILSAIDSFIWGAPLLILLSGTGLYLTLRLGFIQIRYLPRALGYLFKKDKGGKGDVSSFAALCTALAATIGTGNIVG
VATAVQAGGPGAIFWMWLVALLGMATKYAECLLAVKYRVCDKNGFMAGGPMYYIERGLGIKWLAKLFALFGVMVAFFGIG
TFPQVNAITHAMQDTFNIPVLVTAIIVTLLVGLIILGGVKRIATASSVIVPFMAILYVTTSLVIILLNIEKVLDAILLII
DSAFDPQAALGGAVGFTVMKAIQSGVARGIFSNESGLGSAPIAAAAAQTREPVRQGLISMTGTFLDTIIVCTMTGIVLVL
TGAWNNPELAGATVTNYAFAQGLGTSIGATIVTVGLLFFAFTTILGWCYYGERCFVYLVGIRGVKLYRLAYIMLVGLGAF
LHLNLIWIIADIVNGLMAFPNLIALIGLRKVIIEETKDYFQRLKINHYDQDEVIK

Specific function: Unknown

COG id: COG1115

COG function: function code E; Na+/alanine symporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:alanine (SAF) symporter family [H]

Homologues:

Organism=Escherichia coli, GI1786188, Length=450, Percent_Identity=39.5555555555556, Blast_Score=282, Evalue=4e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001463 [H]

Pfam domain/function: PF01235 Na_Ala_symp [H]

EC number: NA

Molecular weight: Translated: 48797; Mature: 48666

Theoretical pI: Translated: 9.05; Mature: 9.05

Prosite motif: PS00873 NA_ALANINE_SYMP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIESILSAIDSFIWGAPLLILLSGTGLYLTLRLGFIQIRYLPRALGYLFKKDKGGKGDV
CCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
SSFAALCTALAATIGTGNIVGVATAVQAGGPGAIFWMWLVALLGMATKYAECLLAVKYRV
HHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CDKNGFMAGGPMYYIERGLGIKWLAKLFALFGVMVAFFGIGTFPQVNAITHAMQDTFNIP
CCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCH
VLVTAIIVTLLVGLIILGGVKRIATASSVIVPFMAILYVTTSLVIILLNIEKVLDAILLI
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IDSAFDPQAALGGAVGFTVMKAIQSGVARGIFSNESGLGSAPIAAAAAQTREPVRQGLIS
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
MTGTFLDTIIVCTMTGIVLVLTGAWNNPELAGATVTNYAFAQGLGTSIGATIVTVGLLFF
HHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHH
AFTTILGWCYYGERCFVYLVGIRGVKLYRLAYIMLVGLGAFLHLNLIWIIADIVNGLMAF
HHHHHHHHHHHCCHHEEEEEHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PNLIALIGLRKVIIEETKDYFQRLKINHYDQDEVIK
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCC
>Mature Secondary Structure 
TIESILSAIDSFIWGAPLLILLSGTGLYLTLRLGFIQIRYLPRALGYLFKKDKGGKGDV
CHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
SSFAALCTALAATIGTGNIVGVATAVQAGGPGAIFWMWLVALLGMATKYAECLLAVKYRV
HHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CDKNGFMAGGPMYYIERGLGIKWLAKLFALFGVMVAFFGIGTFPQVNAITHAMQDTFNIP
CCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCH
VLVTAIIVTLLVGLIILGGVKRIATASSVIVPFMAILYVTTSLVIILLNIEKVLDAILLI
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IDSAFDPQAALGGAVGFTVMKAIQSGVARGIFSNESGLGSAPIAAAAAQTREPVRQGLIS
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
MTGTFLDTIIVCTMTGIVLVLTGAWNNPELAGATVTNYAFAQGLGTSIGATIVTVGLLFF
HHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHH
AFTTILGWCYYGERCFVYLVGIRGVKLYRLAYIMLVGLGAFLHLNLIWIIADIVNGLMAF
HHHHHHHHHHHCCHHEEEEEHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PNLIALIGLRKVIIEETKDYFQRLKINHYDQDEVIK
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; L-alanine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-alanine [Periplasm] = Na (I) [Cytoplasm] + L-alanine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]