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The map label for this gene is nrfA [H]

Identifier: 148826318

GI number: 148826318

Start: 1333973

End: 1335499

Strand: Direct

Name: nrfA [H]

Synonym: CGSHiEE_06735

Alternate gene names: 148826318

Gene position: 1333973-1335499 (Clockwise)

Preceding gene: 148826317

Following gene: 148826319

Centisome position: 73.58

GC content: 40.93

Gene sequence:

>1527_bases
GTGAACGCATTCAAAAAAAGCCTTATTGTGGCAGCGTCTTTCGCGAGTTTAAGCTTATTTAACTCAGCCACTGCTGAGCT
TGTATATAAGCCATTAGAACAACCTGTTGAACCCGCAAAACCTGATTTGAAAATTGAATCTGTAAATGAAAAATTTGCAG
AAAAATATCCAAATCAATATAACAGCTGGTGTTCTACAGCAAACGGCGATGGCGAAAACATTATTTATGCTGATGAAGAA
AATCCGCGCCTAATCGTACTTTGGGGTGGTTATGCATTTGCAAAAGAATATAACGCACCACGCGGTCACTTTTATGCGGT
AACAGATGTGCGTAATATTTTACGTACAGGTGCGCCAAAAACTGCAAATGATGGCCCTCAAGCTATGGCATGTTGGACAT
GTAAAGGTCCTGATGTTCCACGTTTAATCGCGGAATGGGGTGAGAAAGATTATTTCAATGCGAAATGGGCAAAGGGAGGG
CCTGAAATCGTGAACTCAATTGGCTGTGCTGACTGCCACGACACAACCTCTAAAGATTTTGCAGAAGGCAAACCTGCATT
ACGTATTGCTCGTCCACACGTATTACGCGCCCTTGATGCTTTAGAAAAAGCCACTGCTGAAAAAGATAAAGCAGAAGGTC
GCCCACACAACAATTTAAGTTTCAACACGGCAGCGCGTACTGAAAAACGTGCAGAAATTTGCGCAAACTGCCACGTTGAA
TACTATTTTGCAGGGGACATCAAGCAAGTAACTTTCCCTTGGGATAATGGTCAAACCGTAGATGATATTGAAAAATATTA
TGATGATATTGGTTTCACTGACTGGACTCACTCACTTTCTAAAGCACCAATGTTAAAAGCACAACACCCTGACTTTGAAA
TTTGGTCTTTAGGTGTGCACGGTAAAAATGAGGTAACTTGTGTAGATTGCCATATGCCAAAAGTACAAGGTGCGGACGGT
AAAGTTTACACCGATCACCAAATTCAAAATCCATTTGAGGCATTTGACAGCACTTGTGCAAATTGTCACGATCAAAGCAA
AGAAAAATTACGCGATATTGTTACTTCACGTAAAAAAGAAGTGAAAGATGTAATGGGTCGTTTAGAAGACCAAGTTGTCA
AAGCTCACTTTGAAGCAAAAGAGGCTTGGGACGCAGGTGCAACTAAAGAAGAAATGGAAGCAGCATTAATGGATATTCGT
CATGCTCAATGGCGTTGGGATTACACTGCTGCAAGCCATGGTGGTCATATGCATGCGCCTGAAGTAGTACTTCGTGTATT
AGCTTCTGGCTTAGATAAAGTAGCAGACGCACGTACTAAACTTGCTGTAATCTTAACTAAACGCGGTGTGAAAACTCCAG
TTCAAATCCCAGATATTTCAACTGCTGATAAAGCGTGGAAAGTAATGGGTATTGATATTGAAAAAGAACGTAAAGCGAAA
GAAGAATTCTTAAAAACTGTGGTACCACAATGGGAACAACAAGCTAGAGAAAAAGGCTTACTAGTTGATCCACCAGCACA
AAAATAA

Upstream 100 bases:

>100_bases
TACGCTCGAATGAAAATATATATCTACATTTGTGTGGATATTAAGAATAATTCTCAACTTTCTACTTAACCTTTTTATTA
CAACAAATGGAGTGATTATC

Downstream 100 bases:

>100_bases
ATGAAAAATGCACCGCACTTTTTAGTGCAAACTGGAAAGTGCGGTGATTTTCAAGGTCAAATTTGAGGTAACCCAAATGA
ATTTAACTTCTTTAATCAAC

Product: cytochrome c552

Products: NA

Alternate protein names: Ammonia-forming cytochrome c nitrite reductase; Cytochrome c nitrite reductase [H]

Number of amino acids: Translated: 508; Mature: 508

Protein sequence:

>508_residues
MNAFKKSLIVAASFASLSLFNSATAELVYKPLEQPVEPAKPDLKIESVNEKFAEKYPNQYNSWCSTANGDGENIIYADEE
NPRLIVLWGGYAFAKEYNAPRGHFYAVTDVRNILRTGAPKTANDGPQAMACWTCKGPDVPRLIAEWGEKDYFNAKWAKGG
PEIVNSIGCADCHDTTSKDFAEGKPALRIARPHVLRALDALEKATAEKDKAEGRPHNNLSFNTAARTEKRAEICANCHVE
YYFAGDIKQVTFPWDNGQTVDDIEKYYDDIGFTDWTHSLSKAPMLKAQHPDFEIWSLGVHGKNEVTCVDCHMPKVQGADG
KVYTDHQIQNPFEAFDSTCANCHDQSKEKLRDIVTSRKKEVKDVMGRLEDQVVKAHFEAKEAWDAGATKEEMEAALMDIR
HAQWRWDYTAASHGGHMHAPEVVLRVLASGLDKVADARTKLAVILTKRGVKTPVQIPDISTADKAWKVMGIDIEKERKAK
EEFLKTVVPQWEQQAREKGLLVDPPAQK

Sequences:

>Translated_508_residues
MNAFKKSLIVAASFASLSLFNSATAELVYKPLEQPVEPAKPDLKIESVNEKFAEKYPNQYNSWCSTANGDGENIIYADEE
NPRLIVLWGGYAFAKEYNAPRGHFYAVTDVRNILRTGAPKTANDGPQAMACWTCKGPDVPRLIAEWGEKDYFNAKWAKGG
PEIVNSIGCADCHDTTSKDFAEGKPALRIARPHVLRALDALEKATAEKDKAEGRPHNNLSFNTAARTEKRAEICANCHVE
YYFAGDIKQVTFPWDNGQTVDDIEKYYDDIGFTDWTHSLSKAPMLKAQHPDFEIWSLGVHGKNEVTCVDCHMPKVQGADG
KVYTDHQIQNPFEAFDSTCANCHDQSKEKLRDIVTSRKKEVKDVMGRLEDQVVKAHFEAKEAWDAGATKEEMEAALMDIR
HAQWRWDYTAASHGGHMHAPEVVLRVLASGLDKVADARTKLAVILTKRGVKTPVQIPDISTADKAWKVMGIDIEKERKAK
EEFLKTVVPQWEQQAREKGLLVDPPAQK
>Mature_508_residues
MNAFKKSLIVAASFASLSLFNSATAELVYKPLEQPVEPAKPDLKIESVNEKFAEKYPNQYNSWCSTANGDGENIIYADEE
NPRLIVLWGGYAFAKEYNAPRGHFYAVTDVRNILRTGAPKTANDGPQAMACWTCKGPDVPRLIAEWGEKDYFNAKWAKGG
PEIVNSIGCADCHDTTSKDFAEGKPALRIARPHVLRALDALEKATAEKDKAEGRPHNNLSFNTAARTEKRAEICANCHVE
YYFAGDIKQVTFPWDNGQTVDDIEKYYDDIGFTDWTHSLSKAPMLKAQHPDFEIWSLGVHGKNEVTCVDCHMPKVQGADG
KVYTDHQIQNPFEAFDSTCANCHDQSKEKLRDIVTSRKKEVKDVMGRLEDQVVKAHFEAKEAWDAGATKEEMEAALMDIR
HAQWRWDYTAASHGGHMHAPEVVLRVLASGLDKVADARTKLAVILTKRGVKTPVQIPDISTADKAWKVMGIDIEKERKAK
EEFLKTVVPQWEQQAREKGLLVDPPAQK

Specific function: Plays a role in nitrite reduction [H]

COG id: COG3303

COG function: function code P; Formate-dependent nitrite reductase, periplasmic cytochrome c552 subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cytochrome c-552 family [H]

Homologues:

Organism=Escherichia coli, GI1790506, Length=469, Percent_Identity=55.4371002132196, Blast_Score=563, Evalue=1e-162,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003321
- InterPro:   IPR017570
- InterPro:   IPR011031 [H]

Pfam domain/function: PF02335 Cytochrom_C552 [H]

EC number: =1.7.2.2 [H]

Molecular weight: Translated: 56849; Mature: 56849

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS51008 MULTIHEME_CYTC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAFKKSLIVAASFASLSLFNSATAELVYKPLEQPVEPAKPDLKIESVNEKFAEKYPNQY
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHCCHHH
NSWCSTANGDGENIIYADEENPRLIVLWGGYAFAKEYNAPRGHFYAVTDVRNILRTGAPK
HHHHCCCCCCCCEEEEECCCCCEEEEEECCCHHHHHCCCCCCCEEEHHHHHHHHHCCCCC
TANDGPQAMACWTCKGPDVPRLIAEWGEKDYFNAKWAKGGPEIVNSIGCADCHDTTSKDF
CCCCCCCEEEEEECCCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCHH
AEGKPALRIARPHVLRALDALEKATAEKDKAEGRPHNNLSFNTAARTEKRAEICANCHVE
HCCCCCEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCEE
YYFAGDIKQVTFPWDNGQTVDDIEKYYDDIGFTDWTHSLSKAPMLKAQHPDFEIWSLGVH
EEEECCCEEEEECCCCCCCHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCEEEEECCC
GKNEVTCVDCHMPKVQGADGKVYTDHQIQNPFEAFDSTCANCHDQSKEKLRDIVTSRKKE
CCCCEEEEECCCCCCCCCCCCEEECHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VKDVMGRLEDQVVKAHFEAKEAWDAGATKEEMEAALMDIRHAQWRWDYTAASHGGHMHAP
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHEEEEECCCCCCCCCCHH
EVVLRVLASGLDKVADARTKLAVILTKRGVKTPVQIPDISTADKAWKVMGIDIEKERKAK
HHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHH
EEFLKTVVPQWEQQAREKGLLVDPPAQK
HHHHHHHCCHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MNAFKKSLIVAASFASLSLFNSATAELVYKPLEQPVEPAKPDLKIESVNEKFAEKYPNQY
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHCCHHH
NSWCSTANGDGENIIYADEENPRLIVLWGGYAFAKEYNAPRGHFYAVTDVRNILRTGAPK
HHHHCCCCCCCCEEEEECCCCCEEEEEECCCHHHHHCCCCCCCEEEHHHHHHHHHCCCCC
TANDGPQAMACWTCKGPDVPRLIAEWGEKDYFNAKWAKGGPEIVNSIGCADCHDTTSKDF
CCCCCCCEEEEEECCCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCHH
AEGKPALRIARPHVLRALDALEKATAEKDKAEGRPHNNLSFNTAARTEKRAEICANCHVE
HCCCCCEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCEE
YYFAGDIKQVTFPWDNGQTVDDIEKYYDDIGFTDWTHSLSKAPMLKAQHPDFEIWSLGVH
EEEECCCEEEEECCCCCCCHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCEEEEECCC
GKNEVTCVDCHMPKVQGADGKVYTDHQIQNPFEAFDSTCANCHDQSKEKLRDIVTSRKKE
CCCCEEEEECCCCCCCCCCCCEEECHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VKDVMGRLEDQVVKAHFEAKEAWDAGATKEEMEAALMDIRHAQWRWDYTAASHGGHMHAP
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHEEEEECCCCCCCCCCHH
EVVLRVLASGLDKVADARTKLAVILTKRGVKTPVQIPDISTADKAWKVMGIDIEKERKAK
HHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHH
EEFLKTVVPQWEQQAREKGLLVDPPAQK
HHHHHHHCCHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA