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The map label for this gene is ilvE [H]

Identifier: 148826198

GI number: 148826198

Start: 1208037

End: 1209068

Strand: Direct

Name: ilvE [H]

Synonym: CGSHiEE_06075

Alternate gene names: 148826198

Gene position: 1208037-1209068 (Clockwise)

Preceding gene: 148826190

Following gene: 148826200

Centisome position: 66.63

GC content: 43.41

Gene sequence:

>1032_bases
ATGAAAGATTTAGACTGGAATAACCTTGGTTTTAGTTATATTAAAACAGATTACCGCTTCATTGCTCACTGGAAAGATGG
CAAATGGGACGAAGGCAAACTCACAACAGACAATACCTTACATATTCACGAAGGTTCTACCGCACTTCACTACGGTCAGC
AATGTTTTGAAGGCTTAAAGGCTTACCGTTGCAAAGATGGTTCAATCAACTTATTCCGCCCGCAAGCAAATGCAGAGCGT
ATGCAACGTACCGCAGACCGTCTTTTAATGCCACGCGTACCAACTGAATTATTCGTGCGTGCATGTAAAGAAGTGGTTAA
AGCTAACCAAGATTGGTTAGGTCCTTACGGTTCTGGTGCAACCTTATACTTACGTCCATTCTTAATTGGCGTGGGCGAAA
ATATCGGCGTGAAAACGGCACCAGAGTTTATTTTCTCTGTTTTTTGTTGCCCAGTGGGTGCTTACTTCAAAGGTGGTTTG
GCTCCATCAAACTTTATCACGACTGATTATGACCGTGCTGCACCAATGGGCACTGGTGGTGTGAAAGTGGGCGGGAACTA
CGCAGCAAGCTTACTTCCACACGAATTAGTCGCAGAACAAGGTACGCCAGAACGTAAATTTGCGGATGCAATCTATTTAG
ATCCAAAAACTCACACCAAAATTGAAGAAGTCGGTGCGGCAAACTTCTTTGGGATCACAAAAGACAATAAATTCATCACA
CCAAAATCAGAATCTATTTTACCAAGTATCACTAAATACTCTCTTTTACATATCGCAAAAGAGCGTTTAGGTATGGAAGC
AATTGAAGGCGATGTGTATATCGATCAACTCGATCAATTCGTAGAAGCGGGTGCGTGCGGTACAGCTGCGGTGATTACTC
CTGTGGGCGGCATTCAACACAACGGAAAATTCCACGTATTCTATTCAGAAACTGAAGTAGGCCCTGTTACCCGTCGTCTT
TACGATGAATTAACAGGCATCCAATTCGGTGATGTCGAAGCCCCTGAAGGCTGGATTGTGAAAGTAGAATAA

Upstream 100 bases:

>100_bases
CTAAAAATTGCATAATTTCGAGAATTTATTATTTAAATCAGTTCAGGAATGGTGTAGATTAAACGGCATTTCATTCAAAA
AATAATATTAAGGAAGTAAA

Downstream 100 bases:

>100_bases
TTTCTACCAATAGAAAAACGAAAGTGCGGTTAAAAATCAACCGCACTTTTTTATTGATTCAATCCCCATTGGCGAGAATC
TTTATCAAATTTAACACCTG

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT [H]

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MKDLDWNNLGFSYIKTDYRFIAHWKDGKWDEGKLTTDNTLHIHEGSTALHYGQQCFEGLKAYRCKDGSINLFRPQANAER
MQRTADRLLMPRVPTELFVRACKEVVKANQDWLGPYGSGATLYLRPFLIGVGENIGVKTAPEFIFSVFCCPVGAYFKGGL
APSNFITTDYDRAAPMGTGGVKVGGNYAASLLPHELVAEQGTPERKFADAIYLDPKTHTKIEEVGAANFFGITKDNKFIT
PKSESILPSITKYSLLHIAKERLGMEAIEGDVYIDQLDQFVEAGACGTAAVITPVGGIQHNGKFHVFYSETEVGPVTRRL
YDELTGIQFGDVEAPEGWIVKVE

Sequences:

>Translated_343_residues
MKDLDWNNLGFSYIKTDYRFIAHWKDGKWDEGKLTTDNTLHIHEGSTALHYGQQCFEGLKAYRCKDGSINLFRPQANAER
MQRTADRLLMPRVPTELFVRACKEVVKANQDWLGPYGSGATLYLRPFLIGVGENIGVKTAPEFIFSVFCCPVGAYFKGGL
APSNFITTDYDRAAPMGTGGVKVGGNYAASLLPHELVAEQGTPERKFADAIYLDPKTHTKIEEVGAANFFGITKDNKFIT
PKSESILPSITKYSLLHIAKERLGMEAIEGDVYIDQLDQFVEAGACGTAAVITPVGGIQHNGKFHVFYSETEVGPVTRRL
YDELTGIQFGDVEAPEGWIVKVE
>Mature_343_residues
MKDLDWNNLGFSYIKTDYRFIAHWKDGKWDEGKLTTDNTLHIHEGSTALHYGQQCFEGLKAYRCKDGSINLFRPQANAER
MQRTADRLLMPRVPTELFVRACKEVVKANQDWLGPYGSGATLYLRPFLIGVGENIGVKTAPEFIFSVFCCPVGAYFKGGL
APSNFITTDYDRAAPMGTGGVKVGGNYAASLLPHELVAEQGTPERKFADAIYLDPKTHTKIEEVGAANFFGITKDNKFIT
PKSESILPSITKYSLLHIAKERLGMEAIEGDVYIDQLDQFVEAGACGTAAVITPVGGIQHNGKFHVFYSETEVGPVTRRL
YDELTGIQFGDVEAPEGWIVKVE

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI296010906, Length=345, Percent_Identity=33.3333333333333, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI38176287, Length=345, Percent_Identity=33.3333333333333, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI296010904, Length=345, Percent_Identity=33.3333333333333, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI50658084, Length=336, Percent_Identity=31.25, Blast_Score=159, Evalue=3e-39,
Organism=Homo sapiens, GI258614015, Length=291, Percent_Identity=31.6151202749141, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI296010902, Length=345, Percent_Identity=28.1159420289855, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI296010900, Length=345, Percent_Identity=28.1159420289855, Blast_Score=114, Evalue=2e-25,
Organism=Escherichia coli, GI48994963, Length=328, Percent_Identity=29.2682926829268, Blast_Score=108, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI17568601, Length=358, Percent_Identity=31.2849162011173, Blast_Score=164, Evalue=5e-41,
Organism=Caenorhabditis elegans, GI17565728, Length=348, Percent_Identity=29.5977011494253, Blast_Score=119, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6322608, Length=337, Percent_Identity=31.7507418397626, Blast_Score=162, Evalue=6e-41,
Organism=Saccharomyces cerevisiae, GI6322002, Length=345, Percent_Identity=30.4347826086957, Blast_Score=145, Evalue=7e-36,
Organism=Drosophila melanogaster, GI24641779, Length=350, Percent_Identity=30.2857142857143, Blast_Score=159, Evalue=2e-39,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005786 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 37990; Mature: 37990

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDLDWNNLGFSYIKTDYRFIAHWKDGKWDEGKLTTDNTLHIHEGSTALHYGQQCFEGLK
CCCCCCCCCCHHHEECCEEEEEEECCCCCCCCEECCCCEEEEECCCHHHHHHHHHHHHHH
AYRCKDGSINLFRPQANAERMQRTADRLLMPRVPTELFVRACKEVVKANQDWLGPYGSGA
HEECCCCCEEEECCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCC
TLYLRPFLIGVGENIGVKTAPEFIFSVFCCPVGAYFKGGLAPSNFITTDYDRAAPMGTGG
EEEEEEEEEECCCCCCCCHHHHHHHHHHHHCCHHHHCCCCCCCCCEECCCCCCCCCCCCC
VKVGGNYAASLLPHELVAEQGTPERKFADAIYLDPKTHTKIEEVGAANFFGITKDNKFIT
EEECCCHHHHHCHHHHHHCCCCCCHHHCCEEEECCCCCCHHHHCCCCCEEEECCCCEEEC
PKSESILPSITKYSLLHIAKERLGMEAIEGDVYIDQLDQFVEAGACGTAAVITPVGGIQH
CCCCCCCCHHHHHHHHHHHHHHHCHHHHCCCEEHHHHHHHHHCCCCCCEEEEECCCCCEE
NGKFHVFYSETEVGPVTRRLYDELTGIQFGDVEAPEGWIVKVE
CCEEEEEECCCCCCHHHHHHHHHHHCCEECCCCCCCCCEEEEC
>Mature Secondary Structure
MKDLDWNNLGFSYIKTDYRFIAHWKDGKWDEGKLTTDNTLHIHEGSTALHYGQQCFEGLK
CCCCCCCCCCHHHEECCEEEEEEECCCCCCCCEECCCCEEEEECCCHHHHHHHHHHHHHH
AYRCKDGSINLFRPQANAERMQRTADRLLMPRVPTELFVRACKEVVKANQDWLGPYGSGA
HEECCCCCEEEECCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCC
TLYLRPFLIGVGENIGVKTAPEFIFSVFCCPVGAYFKGGLAPSNFITTDYDRAAPMGTGG
EEEEEEEEEECCCCCCCCHHHHHHHHHHHHCCHHHHCCCCCCCCCEECCCCCCCCCCCCC
VKVGGNYAASLLPHELVAEQGTPERKFADAIYLDPKTHTKIEEVGAANFFGITKDNKFIT
EEECCCHHHHHCHHHHHHCCCCCCHHHCCEEEECCCCCCHHHHCCCCCEEEECCCCEEEC
PKSESILPSITKYSLLHIAKERLGMEAIEGDVYIDQLDQFVEAGACGTAAVITPVGGIQH
CCCCCCCCHHHHHHHHHHHHHHHCHHHHCCCEEHHHHHHHHHCCCCCCEEEEECCCCCEE
NGKFHVFYSETEVGPVTRRLYDELTGIQFGDVEAPEGWIVKVE
CCEEEEEECCCCCCHHHHHHHHHHHCCEECCCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]