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The map label for this gene is lysS [H]

Identifier: 148826181

GI number: 148826181

Start: 1188623

End: 1190131

Strand: Direct

Name: lysS [H]

Synonym: CGSHiEE_05980

Alternate gene names: 148826181

Gene position: 1188623-1190131 (Clockwise)

Preceding gene: 148826180

Following gene: 148826183

Centisome position: 65.56

GC content: 39.63

Gene sequence:

>1509_bases
ATGTCAGAACAAGAAGTTAAAGAATTAGATCTCAATGGCGAAATGCTCGTTCGTCGTGAAAAATTATCCGCATTACGTGC
AAAAGGTAATGCCTTTCCAAACAAGTTTCGTCGTGATGCTCTCGCACAAGATTTACACAATCAATATGATTCAGAAGACG
GGGAAATCTTAAAAGAGAAAGGCGTTGAAGTACAGGTTGCTGGTCGTATTATGACTCGTCGTGCAATGGGTAAAGCAACT
TTTATCACTATTCAAGATATGAGCGGTAAAATCCAGCTTTACGTTGCACGCGATAATTTGCCTGAAGGTGTTTATGCTGA
AGATGTAGGTAACTGGGATTTAGGCGATATCATTGGAGTGAAAGGTACACTTTTCAAAACTAAAACCAATGAATTAACCA
TCAAAACAACTGAAGTTCAACTTTTAACCAAAGCACTTCGTCCATTACCAGACAAATTCCATGGTTTAACCGACCAAGAA
GTTCGTTATCGCCAACGTTATTTAGATTTAATTTCTAACGAAGAATCTCGCCGCACTTTTATTATTCGTTCTAAAGTTGT
CGCGGGGATTCGTGAATATTTCATTTCTAAAGGCTTTATGGAAGTGGAAACGCCAATGTTACAAGTAATTCCAGGCGGTG
CATCTGCACGTCCTTTCGTGACTCATCATAATGCATTAGATGTGGATATGTATTTGCGTATCGCACCTGAACTTTATTTA
AAACGCTTAGTCGTTGGTGGGTTTGAACGAGTATTTGAATTAAACCGTAACTTCCGTAATGAAGGGGTTTCTGTTCGTCA
TAATCCAGAATTTACTATGCTTGAATACTATCAAGCCTATGCAGATTACCACGATTTAATGGATAACACCGAAGAACTTC
TTCGTAAATTAGCGATTGATATTCTTGGTACAACCATTGTGAAATACGGTGATTTAGAATTTGACTTCGGCAAACCATTT
GAGCGTATTACATTACACGATGCAACGGTTAAATATGGTGCTGACAAAGGTATCGTAAAAGAAGATTTATATGACTTTGA
TCGTGCAAAAGCCACGGCAGAACGCTTAGGTATTGAAGTACAAAAATCTTGGGGTTTAGGCAGCATTGTCAATGCAATTT
TTGAAGAAGTAGCTGAACATCATTTAATTCAACCAACCTTCTTAATGGCTCACCCTGCGGAAATTTCGCCACTTGCACGT
CGTAATGATGAAAACCCTGAAGTGACTGACCGTTTTGAACTATTTATCGGTGGCCGTGAAATCGGTAATGGTTTCTCAGA
ATTAAATGATGCAGAAGATCAAAATGAACGTTTTGATGCACAGGTTGCTGCGAAAGAAGCGGGCGATGATGAAGCGATGT
TTAAAGATGAAGACTTTGTTGTTGCACTAGAACACGGCTTACCTCCAACAGCTGGGGAAGGTTTAGGTATCGACCGCTTG
GCGATGCTTTATGCAAATGCACCATCCATTCGTGATGTGATCTTGTTCCCTGCAATGCGTCAGAAATAA

Upstream 100 bases:

>100_bases
GGATAAGTAAAGAAGCGTTACTTATCCCATCATAAGAAATAACCCAAAAGTGCGGTTATTTTTGAAGGTATTTTTAAAGT
TAAAATTAAAGGTAAACAAA

Downstream 100 bases:

>100_bases
TAAAGATACAAACAAAAGCCACCAAATGGTGGCTTTTTTATCGAAACATAAAAAGGGAGCTAACGCTCCCCTTACTTTTT
AATGATTAACCATTAATAAA

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS [H]

Number of amino acids: Translated: 502; Mature: 501

Protein sequence:

>502_residues
MSEQEVKELDLNGEMLVRREKLSALRAKGNAFPNKFRRDALAQDLHNQYDSEDGEILKEKGVEVQVAGRIMTRRAMGKAT
FITIQDMSGKIQLYVARDNLPEGVYAEDVGNWDLGDIIGVKGTLFKTKTNELTIKTTEVQLLTKALRPLPDKFHGLTDQE
VRYRQRYLDLISNEESRRTFIIRSKVVAGIREYFISKGFMEVETPMLQVIPGGASARPFVTHHNALDVDMYLRIAPELYL
KRLVVGGFERVFELNRNFRNEGVSVRHNPEFTMLEYYQAYADYHDLMDNTEELLRKLAIDILGTTIVKYGDLEFDFGKPF
ERITLHDATVKYGADKGIVKEDLYDFDRAKATAERLGIEVQKSWGLGSIVNAIFEEVAEHHLIQPTFLMAHPAEISPLAR
RNDENPEVTDRFELFIGGREIGNGFSELNDAEDQNERFDAQVAAKEAGDDEAMFKDEDFVVALEHGLPPTAGEGLGIDRL
AMLYANAPSIRDVILFPAMRQK

Sequences:

>Translated_502_residues
MSEQEVKELDLNGEMLVRREKLSALRAKGNAFPNKFRRDALAQDLHNQYDSEDGEILKEKGVEVQVAGRIMTRRAMGKAT
FITIQDMSGKIQLYVARDNLPEGVYAEDVGNWDLGDIIGVKGTLFKTKTNELTIKTTEVQLLTKALRPLPDKFHGLTDQE
VRYRQRYLDLISNEESRRTFIIRSKVVAGIREYFISKGFMEVETPMLQVIPGGASARPFVTHHNALDVDMYLRIAPELYL
KRLVVGGFERVFELNRNFRNEGVSVRHNPEFTMLEYYQAYADYHDLMDNTEELLRKLAIDILGTTIVKYGDLEFDFGKPF
ERITLHDATVKYGADKGIVKEDLYDFDRAKATAERLGIEVQKSWGLGSIVNAIFEEVAEHHLIQPTFLMAHPAEISPLAR
RNDENPEVTDRFELFIGGREIGNGFSELNDAEDQNERFDAQVAAKEAGDDEAMFKDEDFVVALEHGLPPTAGEGLGIDRL
AMLYANAPSIRDVILFPAMRQK
>Mature_501_residues
SEQEVKELDLNGEMLVRREKLSALRAKGNAFPNKFRRDALAQDLHNQYDSEDGEILKEKGVEVQVAGRIMTRRAMGKATF
ITIQDMSGKIQLYVARDNLPEGVYAEDVGNWDLGDIIGVKGTLFKTKTNELTIKTTEVQLLTKALRPLPDKFHGLTDQEV
RYRQRYLDLISNEESRRTFIIRSKVVAGIREYFISKGFMEVETPMLQVIPGGASARPFVTHHNALDVDMYLRIAPELYLK
RLVVGGFERVFELNRNFRNEGVSVRHNPEFTMLEYYQAYADYHDLMDNTEELLRKLAIDILGTTIVKYGDLEFDFGKPFE
RITLHDATVKYGADKGIVKEDLYDFDRAKATAERLGIEVQKSWGLGSIVNAIFEEVAEHHLIQPTFLMAHPAEISPLARR
NDENPEVTDRFELFIGGREIGNGFSELNDAEDQNERFDAQVAAKEAGDDEAMFKDEDFVVALEHGLPPTAGEGLGIDRLA
MLYANAPSIRDVILFPAMRQK

Specific function: Also Can Synthesize A Number Of Adenyl Dinucleotides (In Particular Appppa). These Dinucleotides Have Been Proposed To Act As Modulators Of The Heat-Shock Response And Stress Response. [C]

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI194272210, Length=511, Percent_Identity=41.4872798434442, Blast_Score=383, Evalue=1e-106,
Organism=Homo sapiens, GI5031815, Length=511, Percent_Identity=41.4872798434442, Blast_Score=383, Evalue=1e-106,
Organism=Homo sapiens, GI45439306, Length=464, Percent_Identity=23.2758620689655, Blast_Score=100, Evalue=6e-21,
Organism=Homo sapiens, GI40789249, Length=289, Percent_Identity=27.3356401384083, Blast_Score=90, Evalue=6e-18,
Organism=Escherichia coli, GI1790571, Length=504, Percent_Identity=69.4444444444444, Blast_Score=736, Evalue=0.0,
Organism=Escherichia coli, GI1789256, Length=504, Percent_Identity=69.6428571428571, Blast_Score=731, Evalue=0.0,
Organism=Escherichia coli, GI87082379, Length=321, Percent_Identity=32.7102803738318, Blast_Score=148, Evalue=8e-37,
Organism=Escherichia coli, GI1788173, Length=290, Percent_Identity=26.8965517241379, Blast_Score=95, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI17535925, Length=502, Percent_Identity=41.2350597609562, Blast_Score=362, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI71994340, Length=502, Percent_Identity=41.2350597609562, Blast_Score=361, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI17535927, Length=502, Percent_Identity=41.2350597609562, Blast_Score=361, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI17551876, Length=473, Percent_Identity=23.4672304439746, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI32566633, Length=287, Percent_Identity=26.4808362369338, Blast_Score=84, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6320242, Length=516, Percent_Identity=40.1162790697674, Blast_Score=370, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6324256, Length=480, Percent_Identity=29.7916666666667, Blast_Score=170, Evalue=4e-43,
Organism=Saccharomyces cerevisiae, GI6323011, Length=481, Percent_Identity=25.5717255717256, Blast_Score=102, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24640849, Length=519, Percent_Identity=40.4624277456647, Blast_Score=375, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24640851, Length=519, Percent_Identity=40.4624277456647, Blast_Score=375, Evalue=1e-104,
Organism=Drosophila melanogaster, GI17136276, Length=461, Percent_Identity=23.644251626898, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24584738, Length=272, Percent_Identity=29.4117647058824, Blast_Score=88, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.6 [H]

Molecular weight: Translated: 57005; Mature: 56873

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEQEVKELDLNGEMLVRREKLSALRAKGNAFPNKFRRDALAQDLHNQYDSEDGEILKEK
CCHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHC
GVEVQVAGRIMTRRAMGKATFITIQDMSGKIQLYVARDNLPEGVYAEDVGNWDLGDIIGV
CCEEEEHHHHHHHHHCCCEEEEEEEECCCEEEEEEECCCCCCCCCCCCCCCCCCHHEECC
KGTLFKTKTNELTIKTTEVQLLTKALRPLPDKFHGLTDQEVRYRQRYLDLISNEESRRTF
CCEEEEECCCEEEEEEHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHCCCCCCEEE
IIRSKVVAGIREYFISKGFMEVETPMLQVIPGGASARPFVTHHNALDVDMYLRIAPELYL
EHHHHHHHHHHHHHHHCCCHHHCCCEEEEECCCCCCCCEEEECCCCCHHHHHHHHHHHHH
KRLVVGGFERVFELNRNFRNEGVSVRHNPEFTMLEYYQAYADYHDLMDNTEELLRKLAID
HHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
ILGTTIVKYGDLEFDFGKPFERITLHDATVKYGADKGIVKEDLYDFDRAKATAERLGIEV
HHHHHHHHCCCEEECCCCCCCEEEEEHHHEEECCCCCCHHHHHHHHHHHHHHHHHHCEEE
QKSWGLGSIVNAIFEEVAEHHLIQPTFLMAHPAEISPLARRNDENPEVTDRFELFIGGRE
ECCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHCCCCCCCCCCEEEEEECCHH
IGNGFSELNDAEDQNERFDAQVAAKEAGDDEAMFKDEDFVVALEHGLPPTAGEGLGIDRL
HCCCHHHCCCCCCHHHHHHHHHHHHCCCCCCCEECCCCEEEEEECCCCCCCCCCCCHHHH
AMLYANAPSIRDVILFPAMRQK
HHHHCCCCCHHHEEECCCCCCC
>Mature Secondary Structure 
SEQEVKELDLNGEMLVRREKLSALRAKGNAFPNKFRRDALAQDLHNQYDSEDGEILKEK
CHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHC
GVEVQVAGRIMTRRAMGKATFITIQDMSGKIQLYVARDNLPEGVYAEDVGNWDLGDIIGV
CCEEEEHHHHHHHHHCCCEEEEEEEECCCEEEEEEECCCCCCCCCCCCCCCCCCHHEECC
KGTLFKTKTNELTIKTTEVQLLTKALRPLPDKFHGLTDQEVRYRQRYLDLISNEESRRTF
CCEEEEECCCEEEEEEHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHCCCCCCEEE
IIRSKVVAGIREYFISKGFMEVETPMLQVIPGGASARPFVTHHNALDVDMYLRIAPELYL
EHHHHHHHHHHHHHHHCCCHHHCCCEEEEECCCCCCCCEEEECCCCCHHHHHHHHHHHHH
KRLVVGGFERVFELNRNFRNEGVSVRHNPEFTMLEYYQAYADYHDLMDNTEELLRKLAID
HHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
ILGTTIVKYGDLEFDFGKPFERITLHDATVKYGADKGIVKEDLYDFDRAKATAERLGIEV
HHHHHHHHCCCEEECCCCCCCEEEEEHHHEEECCCCCCHHHHHHHHHHHHHHHHHHCEEE
QKSWGLGSIVNAIFEEVAEHHLIQPTFLMAHPAEISPLARRNDENPEVTDRFELFIGGRE
ECCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHCCCCCCCCCCEEEEEECCHH
IGNGFSELNDAEDQNERFDAQVAAKEAGDDEAMFKDEDFVVALEHGLPPTAGEGLGIDRL
HCCCHHHCCCCCCHHHHHHHHHHHHCCCCCCCEECCCCEEEEEECCCCCCCCCCCCHHHH
AMLYANAPSIRDVILFPAMRQK
HHHHCCCCCHHHEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA