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The map label for this gene is pitB [C]

Identifier: 148826129

GI number: 148826129

Start: 1133678

End: 1134940

Strand: Direct

Name: pitB [C]

Synonym: CGSHiEE_05670

Alternate gene names: 148826129

Gene position: 1133678-1134940 (Clockwise)

Preceding gene: 148826128

Following gene: 148826130

Centisome position: 62.53

GC content: 39.27

Gene sequence:

>1263_bases
ATGGAAATTATTAGTCAATATGGTTCTTGGTTGGTGTGGATCACAGCAGCCTTTGGCTTTTTTATGGCGTTCGGAATTGG
AGCAAATGATGTCTCAAACTCAATGGGTACATCCGTAGGATCTGGTACTGTAACAGCCAAACAAGCAATTATTATTGCGT
TAATTTTTGAATCTGCAGGTGCATATTTAGCTGGTGGCGAAGTAACACAAACCATAAAAAGTGGCGTAATCGACCCAATA
CAATTTGTTGATACACCCGATATTTTAGCCTTAGGTATGCTTTCAACCTTATTTGCATCAGGTGCTTGGTTATTTATTGT
AACCAAAATGGGTTGGCCAGTTTCTGGTATACACACTATTATTGGTGCAATTATTGGATTTGCCTGTATTACGATTGGCC
CTAGTTCTGTAGATTGGTCAAATATTGGCAGCATCGTTGGTAGCTGGTTTATCACGCCTGTCATCTCGGGTATTTTGGCT
TATGCAATCTTTGCAAGTACGCAAAAACTTATTTTTGATACTGAACAGCCATTAAAAAATGCACAAAAATATGGCCCTTA
TTATATGGGGATAACCGTGTTTGTACTTTGTATCGTGACGATGAAAAAAGGCTTAAAACACGTAGGCTTAAATCTTTCAA
ACAGTGAAACTTTAATCATTTCACTTGCTATCAGTCTTATCGGAATGTTTTTCTTCCATTTCTATTTTAAAAGCAAAATC
TTCACTCAATCAGCAAACAAAGGCACTTTTGGTGCCGTAGAAAAAGTCTTCAGTATTTTAATGTTATTAACCGCTTGTGC
GATGGCATTTGCGCACGGTTCTAATGACGTAGCCAATGCAATTGGCCCTCTTTCTGCAGTGGTCTCTATTGTAAATGAAG
GCGGTAAAATTGTTTCAGGTGGAGCCTTAACTTGGTGGATTTTACCCTTAGGTGCATTAGGTATTGCTGTGGGCTTAATT
ACTATGGGACAAAAAGTGATGGCGACTGTTGGATCTGGGATCACCGATTTAACGCCAAGCCGTGGCTTTGCTGCTCAATT
TGCTACTGCAATGACTGTTGTAGTTGCATCAGGCACAGGCTTACCCATCTCAACAACACAAACACTTGTTGGAGCTATTT
TAGGTATCGGTTTTGCACGTGGTATCGCTGCACTGAATTTAACGGTTATCCGAAACATCATTAGTTCTTGGATTGTCACA
TTACCAGCAGGCGCATTTTTCGCCATTATTATTTTCTATGTGCTAAGAACTATCTTTAATTAA

Upstream 100 bases:

>100_bases
TGAATGGGTTGGCGTTTTGGCTGACCAAGCACAACGAGTCGGTTCGCGCATTGAATTAATGCTGGCGCGTTCATAATCAA
CAAAACATAGGAAATTAACT

Downstream 100 bases:

>100_bases
TTGATCTCAATAAAAAGTGCGGTTAATTTTAACCGCACTTTTGTTGTGATGTTTAAGGGGAAAAAATGAAAAAGATCTAT
AAAGCCTTAATCTCATCTTT

Product: putative phosphate permease

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 420; Mature: 420

Protein sequence:

>420_residues
MEIISQYGSWLVWITAAFGFFMAFGIGANDVSNSMGTSVGSGTVTAKQAIIIALIFESAGAYLAGGEVTQTIKSGVIDPI
QFVDTPDILALGMLSTLFASGAWLFIVTKMGWPVSGIHTIIGAIIGFACITIGPSSVDWSNIGSIVGSWFITPVISGILA
YAIFASTQKLIFDTEQPLKNAQKYGPYYMGITVFVLCIVTMKKGLKHVGLNLSNSETLIISLAISLIGMFFFHFYFKSKI
FTQSANKGTFGAVEKVFSILMLLTACAMAFAHGSNDVANAIGPLSAVVSIVNEGGKIVSGGALTWWILPLGALGIAVGLI
TMGQKVMATVGSGITDLTPSRGFAAQFATAMTVVVASGTGLPISTTQTLVGAILGIGFARGIAALNLTVIRNIISSWIVT
LPAGAFFAIIIFYVLRTIFN

Sequences:

>Translated_420_residues
MEIISQYGSWLVWITAAFGFFMAFGIGANDVSNSMGTSVGSGTVTAKQAIIIALIFESAGAYLAGGEVTQTIKSGVIDPI
QFVDTPDILALGMLSTLFASGAWLFIVTKMGWPVSGIHTIIGAIIGFACITIGPSSVDWSNIGSIVGSWFITPVISGILA
YAIFASTQKLIFDTEQPLKNAQKYGPYYMGITVFVLCIVTMKKGLKHVGLNLSNSETLIISLAISLIGMFFFHFYFKSKI
FTQSANKGTFGAVEKVFSILMLLTACAMAFAHGSNDVANAIGPLSAVVSIVNEGGKIVSGGALTWWILPLGALGIAVGLI
TMGQKVMATVGSGITDLTPSRGFAAQFATAMTVVVASGTGLPISTTQTLVGAILGIGFARGIAALNLTVIRNIISSWIVT
LPAGAFFAIIIFYVLRTIFN
>Mature_420_residues
MEIISQYGSWLVWITAAFGFFMAFGIGANDVSNSMGTSVGSGTVTAKQAIIIALIFESAGAYLAGGEVTQTIKSGVIDPI
QFVDTPDILALGMLSTLFASGAWLFIVTKMGWPVSGIHTIIGAIIGFACITIGPSSVDWSNIGSIVGSWFITPVISGILA
YAIFASTQKLIFDTEQPLKNAQKYGPYYMGITVFVLCIVTMKKGLKHVGLNLSNSETLIISLAISLIGMFFFHFYFKSKI
FTQSANKGTFGAVEKVFSILMLLTACAMAFAHGSNDVANAIGPLSAVVSIVNEGGKIVSGGALTWWILPLGALGIAVGLI
TMGQKVMATVGSGITDLTPSRGFAAQFATAMTVVVASGTGLPISTTQTLVGAILGIGFARGIAALNLTVIRNIISSWIVT
LPAGAFFAIIIFYVLRTIFN

Specific function: Potential transporter for phosphate [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family [H]

Homologues:

Organism=Homo sapiens, GI5803173, Length=250, Percent_Identity=34.4, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI31543630, Length=291, Percent_Identity=34.020618556701, Blast_Score=145, Evalue=7e-35,
Organism=Caenorhabditis elegans, GI25146401, Length=463, Percent_Identity=33.0453563714903, Blast_Score=233, Evalue=1e-61,
Organism=Caenorhabditis elegans, GI71981576, Length=454, Percent_Identity=32.15859030837, Blast_Score=216, Evalue=2e-56,
Organism=Caenorhabditis elegans, GI17557328, Length=494, Percent_Identity=26.3157894736842, Blast_Score=155, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI17539280, Length=252, Percent_Identity=37.3015873015873, Blast_Score=142, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI32566716, Length=235, Percent_Identity=31.4893617021277, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17536725, Length=166, Percent_Identity=34.9397590361446, Blast_Score=110, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6319773, Length=178, Percent_Identity=35.3932584269663, Blast_Score=108, Evalue=1e-24,
Organism=Drosophila melanogaster, GI21356511, Length=197, Percent_Identity=40.6091370558376, Blast_Score=158, Evalue=8e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 44026; Mature: 44026

Theoretical pI: Translated: 9.05; Mature: 9.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIISQYGSWLVWITAAFGFFMAFGIGANDVSNSMGTSVGSGTVTAKQAIIIALIFESAG
CCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCC
AYLAGGEVTQTIKSGVIDPIQFVDTPDILALGMLSTLFASGAWLFIVTKMGWPVSGIHTI
CEEECCHHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHH
IGAIIGFACITIGPSSVDWSNIGSIVGSWFITPVISGILAYAIFASTQKLIFDTEQPLKN
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHH
AQKYGPYYMGITVFVLCIVTMKKGLKHVGLNLSNSETLIISLAISLIGMFFFHFYFKSKI
HHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
FTQSANKGTFGAVEKVFSILMLLTACAMAFAHGSNDVANAIGPLSAVVSIVNEGGKIVSG
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEC
GALTWWILPLGALGIAVGLITMGQKVMATVGSGITDLTPSRGFAAQFATAMTVVVASGTG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHEECCCC
LPISTTQTLVGAILGIGFARGIAALNLTVIRNIISSWIVTLPAGAFFAIIIFYVLRTIFN
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEIISQYGSWLVWITAAFGFFMAFGIGANDVSNSMGTSVGSGTVTAKQAIIIALIFESAG
CCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCC
AYLAGGEVTQTIKSGVIDPIQFVDTPDILALGMLSTLFASGAWLFIVTKMGWPVSGIHTI
CEEECCHHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHH
IGAIIGFACITIGPSSVDWSNIGSIVGSWFITPVISGILAYAIFASTQKLIFDTEQPLKN
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHH
AQKYGPYYMGITVFVLCIVTMKKGLKHVGLNLSNSETLIISLAISLIGMFFFHFYFKSKI
HHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
FTQSANKGTFGAVEKVFSILMLLTACAMAFAHGSNDVANAIGPLSAVVSIVNEGGKIVSG
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEC
GALTWWILPLGALGIAVGLITMGQKVMATVGSGITDLTPSRGFAAQFATAMTVVVASGTG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHEECCCC
LPISTTQTLVGAILGIGFARGIAALNLTVIRNIISSWIVTLPAGAFFAIIIFYVLRTIFN
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]