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The map label for this gene is gltS [H]

Identifier: 148826056

GI number: 148826056

Start: 1051557

End: 1052771

Strand: Direct

Name: gltS [H]

Synonym: CGSHiEE_05230

Alternate gene names: 148826056

Gene position: 1051557-1052771 (Clockwise)

Preceding gene: 148826055

Following gene: 148826058

Centisome position: 58.0

GC content: 37.86

Gene sequence:

>1215_bases
ATGAGCTATACTTTTAGCACGTATGAAACCCTTGCATTAGCAAGCTTAGTTTTGCTATTGGGCTATTTTTTGGTTAAACG
CATTAATGTATTAAAAACATTTAACATTCCAGAGCCAGTTGTTGGCGGCTTTATTGTTGCCATTGGTCTTTTAATTTGGC
ACAAAATTGATGGAACATCCTTTAACTTCGATAAAAATCTACAAACCACAATGATGTTAGTGTTTTTTACTTCTATCGGT
TTAAGTGCCAACTTTTCCCGTCTAATTAAAGGCGGAAAGCCACTCGTTGTATTTCTATTCATTGCTGCATTATTAATCTT
CGGACAAAACGTTATTGGCATTGCAAGTTCAATGGCATTAGGAATCCATCCCGCTTATGGTTTACTTGCAGGTTCAGTAA
CCTTAACAGGTGGTCACGGCACTGGAGCTGCTTGGGCGGATACATTTGCACATCAATTTAATCTACAAGGTGCAACTGAA
ATTGCCATTGCCTGTGCAACTTTCGGTTTAGTATTCGGTGGTATCATCGGTGGTCCTGTCGCTCGTTTCTTATTAAATCG
CCAAAAACAAGGCGAAAATCCTGAAAATGATGAAGTTGATGACATTCAAGAAGTATTTGAACACCCAACTTATAAACGTA
AAATTACTGCACGTTCATTAATTGAAACAATTGCAATGATTTCAGTATGTTTATTAATTGGTCAATATTTAGATGTACAA
ACAAAAGGTACTGCACTCCAATTACCAACTTTCGTATGGTGTTTATTCACTGGTGTTATTGTCCGCAATATTTTAAGCAA
CATTTTCCGTTTCCAAGTAGCAGAATCCGCTATTGACGTATTAGGTAGCGTAGGCTTATCCATCTTTTTAGCAATTGCTT
TAATGTCATTAAGACTTTGGGAACTTGCAGGTTTAGCAATTGATGTATTAATTGTTCTTGCTATTCAAGTCGCATTTATG
GCTGCCTTTGCAATCTTCATTACTTATCGAGCAATGGGTAAAGATTACGATGCCGTCGTATTAAGTGCTGGACACTGTGG
TTTTGGTTTAGGCGCAACCCCAACAGCAATCGCTAATATGCAAGCTGTTACCAGCCGTTTTGGACCATCACACAAAGCAT
TTTTAATCGTTCCAATGGTGGGTGCATTCTTTATCGACTTAATCAACGCAGCATTATTGAAAGTCTCATTTGCAGTTGTA
AATATGCTCGCGTAA

Upstream 100 bases:

>100_bases
GTTCGACATTACCACGTGGATTACATACCAATCTTGAAATTATGGTAGTCGAGCCGTAACACAACACATCACTTAAACAT
AACGTAGGGAGATCCAATTT

Downstream 100 bases:

>100_bases
AGTGCGGTTGAAATTGACAATATTTTAAAAAATGCCTTTATCTTAAATTGATAAAGGCATTTATTTCAATATAATCAATT
ATTTCAGCTGCAATATCAAC

Product: sodium/glutamate symport carrier protein

Products: Na (I) [Cytoplasm]; L-glutamate [Cytoplasm] [C]

Alternate protein names: Glutamate permease [H]

Number of amino acids: Translated: 404; Mature: 403

Protein sequence:

>404_residues
MSYTFSTYETLALASLVLLLGYFLVKRINVLKTFNIPEPVVGGFIVAIGLLIWHKIDGTSFNFDKNLQTTMMLVFFTSIG
LSANFSRLIKGGKPLVVFLFIAALLIFGQNVIGIASSMALGIHPAYGLLAGSVTLTGGHGTGAAWADTFAHQFNLQGATE
IAIACATFGLVFGGIIGGPVARFLLNRQKQGENPENDEVDDIQEVFEHPTYKRKITARSLIETIAMISVCLLIGQYLDVQ
TKGTALQLPTFVWCLFTGVIVRNILSNIFRFQVAESAIDVLGSVGLSIFLAIALMSLRLWELAGLAIDVLIVLAIQVAFM
AAFAIFITYRAMGKDYDAVVLSAGHCGFGLGATPTAIANMQAVTSRFGPSHKAFLIVPMVGAFFIDLINAALLKVSFAVV
NMLA

Sequences:

>Translated_404_residues
MSYTFSTYETLALASLVLLLGYFLVKRINVLKTFNIPEPVVGGFIVAIGLLIWHKIDGTSFNFDKNLQTTMMLVFFTSIG
LSANFSRLIKGGKPLVVFLFIAALLIFGQNVIGIASSMALGIHPAYGLLAGSVTLTGGHGTGAAWADTFAHQFNLQGATE
IAIACATFGLVFGGIIGGPVARFLLNRQKQGENPENDEVDDIQEVFEHPTYKRKITARSLIETIAMISVCLLIGQYLDVQ
TKGTALQLPTFVWCLFTGVIVRNILSNIFRFQVAESAIDVLGSVGLSIFLAIALMSLRLWELAGLAIDVLIVLAIQVAFM
AAFAIFITYRAMGKDYDAVVLSAGHCGFGLGATPTAIANMQAVTSRFGPSHKAFLIVPMVGAFFIDLINAALLKVSFAVV
NMLA
>Mature_403_residues
SYTFSTYETLALASLVLLLGYFLVKRINVLKTFNIPEPVVGGFIVAIGLLIWHKIDGTSFNFDKNLQTTMMLVFFTSIGL
SANFSRLIKGGKPLVVFLFIAALLIFGQNVIGIASSMALGIHPAYGLLAGSVTLTGGHGTGAAWADTFAHQFNLQGATEI
AIACATFGLVFGGIIGGPVARFLLNRQKQGENPENDEVDDIQEVFEHPTYKRKITARSLIETIAMISVCLLIGQYLDVQT
KGTALQLPTFVWCLFTGVIVRNILSNIFRFQVAESAIDVLGSVGLSIFLAIALMSLRLWELAGLAIDVLIVLAIQVAFMA
AFAIFITYRAMGKDYDAVVLSAGHCGFGLGATPTAIANMQAVTSRFGPSHKAFLIVPMVGAFFIDLINAALLKVSFAVVN
MLA

Specific function: Catalyzes the sodium-dependent uptake of extracellular glutamate [H]

COG id: COG0786

COG function: function code E; Na+/glutamate symporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gltS symporter family [H]

Homologues:

Organism=Escherichia coli, GI1790085, Length=375, Percent_Identity=54.1333333333333, Blast_Score=363, Evalue=1e-101,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004445 [H]

Pfam domain/function: PF03616 Glt_symporter [H]

EC number: NA

Molecular weight: Translated: 43404; Mature: 43273

Theoretical pI: Translated: 7.99; Mature: 7.99

Prosite motif: PS00134 TRYPSIN_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYTFSTYETLALASLVLLLGYFLVKRINVLKTFNIPEPVVGGFIVAIGLLIWHKIDGTS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCC
FNFDKNLQTTMMLVFFTSIGLSANFSRLIKGGKPLVVFLFIAALLIFGQNVIGIASSMAL
CCCCCCHHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHH
GIHPAYGLLAGSVTLTGGHGTGAAWADTFAHQFNLQGATEIAIACATFGLVFGGIIGGPV
CCCCHHHHHCCCEEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
ARFLLNRQKQGENPENDEVDDIQEVFEHPTYKRKITARSLIETIAMISVCLLIGQYLDVQ
HHHHHHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TKGTALQLPTFVWCLFTGVIVRNILSNIFRFQVAESAIDVLGSVGLSIFLAIALMSLRLW
CCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELAGLAIDVLIVLAIQVAFMAAFAIFITYRAMGKDYDAVVLSAGHCGFGLGATPTAIANM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHH
QAVTSRFGPSHKAFLIVPMVGAFFIDLINAALLKVSFAVVNMLA
HHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SYTFSTYETLALASLVLLLGYFLVKRINVLKTFNIPEPVVGGFIVAIGLLIWHKIDGTS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCC
FNFDKNLQTTMMLVFFTSIGLSANFSRLIKGGKPLVVFLFIAALLIFGQNVIGIASSMAL
CCCCCCHHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHH
GIHPAYGLLAGSVTLTGGHGTGAAWADTFAHQFNLQGATEIAIACATFGLVFGGIIGGPV
CCCCHHHHHCCCEEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
ARFLLNRQKQGENPENDEVDDIQEVFEHPTYKRKITARSLIETIAMISVCLLIGQYLDVQ
HHHHHHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TKGTALQLPTFVWCLFTGVIVRNILSNIFRFQVAESAIDVLGSVGLSIFLAIALMSLRLW
CCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELAGLAIDVLIVLAIQVAFMAAFAIFITYRAMGKDYDAVVLSAGHCGFGLGATPTAIANM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHH
QAVTSRFGPSHKAFLIVPMVGAFFIDLINAALLKVSFAVVNMLA
HHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; L-glutamate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-glutamate [Periplasm] = Na (I) [Cytoplasm] + L-glutamate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]