Click here to switch to the map view.

The map label for this gene is dacB [H]

Identifier: 148826012

GI number: 148826012

Start: 1005771

End: 1007210

Strand: Direct

Name: dacB [H]

Synonym: CGSHiEE_04955

Alternate gene names: 148826012

Gene position: 1005771-1007210 (Clockwise)

Preceding gene: 148826010

Following gene: 148826013

Centisome position: 55.47

GC content: 37.57

Gene sequence:

>1440_bases
ATGAAAAAATTATCTTCAATTTCCACCGCACTTGGTTCCTTTTTACTTTCAGTGAGTTTTAGCCTACCAACTTTCGCCAA
TATTAATGTTTCAGATTTAACACAAAAATTGCCTGAAGGCTCTAACGCAGGTGTTATTGCTAAAAATATCAATCAAAATC
AAATAATTGCTGATTATAATGGCTCAACCTTTATGCTGCCTGCGAGTACACAAAAAGTTTTTACAGCCGTTGCCGCTAAA
CTTGCATTAGGCGATCAATTCCAATTTGAGACCGCACTTTTGAGTAATGGGAAAATTCAAAATGGGAATTTAGATGGCAA
CTTAATCGTGAGTTTCACAGGCGATCCTGATCTCACAAGAGGTCAGCTTTATAGCTTACTTGCAGAATTAAAAAAACAAG
GCATCAAAAAAATTAATGGCGATTTAGTATTAGATACCTCTGTTTTTTCTAGTCACGACCGAGGATTAGGTTGGATTTGG
AACGATCTCACAATGTGTTTCAACTCTCCACCAGCAGCTGCGAATATTGATAACAACTGTTTTTATGCGGAACTAGATGC
GAATAAAAATCCAGGTGAAATAGTAAAAATTAATGTGCCTGCACAATTTCCAATTCAAGTGTTCGGACAAGTTTATGTAG
CGGATAGCAATGAAGCGCCTTATTGTCAGCTCGATGTTGTTGTACACGACAATAATCGCTATCAAGTAAAAGGATGTTTA
GCCCGTCAATATAAACCTTTTGGCTTAAGTTTTGCCGTGCAAAATACGGATGCTTATGCTGCCGCGATTATTCAACGTCA
ATTAAGAAAATTAGGTATTGAGTTCAATGGCAAAGTCTTGTTACCCCAAAAACCACAACAAGGTCAGCTATTAGCGAAAC
ATTTATCAAAACCATTACCTGATTTATTGAAAAAAATGATGAAAAAATCGGATAACCAAATTGCCGATTCATTATTCCGA
GCGGTGGCATTCAATTACTATAAACGCCCCGCCTCCTTTCAATTAGGCACATTAGCGGTTAAATCCATATTGCAAAAACA
AGGAATTCGTTTTGGTAATAGCATTTTAGCCGATGGTTCGGGTCTATCTCGCCATAATCTTGTGGCACCTAAAACGATGC
TATCCGTTTTAGAATACATCGCCAAAAACGAAGATAAATTACATTTAATGGAAACTTTTCCCATCGCAGGTGTAGATGGC
ACGATCAGTGGGCGAGGTGGATTAATTAGCCCACCATTAGTGAAAAACGTGATTGCTAAAACTGGTTCTTTGAAAGGCGT
TTATAACCTAGCTGGCTTTATGACAAATGCGCGAGGGGAAAAAGTGGCATTTGTACAATTTATTAATGGCTACTCAACGG
GCGATTTAGAAAGCAAAACAAAACGTGCCCCATTAGTACAATTCGAGCGTAATTTATATAACGAACTCTATAAATATTAA

Upstream 100 bases:

>100_bases
TATCAAAAATAAACTTGAAATGAGAAAATGATTTACTCAATTTCATTCGGGGCTTATTATTGTTCGTCTAAAAAAAAATA
GCAACCATAACAAACCTAAT

Downstream 100 bases:

>100_bases
GGTGCTGTAGTAGATAACAACTAAAAATCCCATTTAGGTTAGAATAAACCAATGAGAAAAAGTCGCTTAAGTCAGCATAA
ACAAAATAAACTCATTGAAC

Product: D-alanyl-D-alanine carboxypeptidase/endopeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; D-alanyl-D-alanine endopeptidase; DD-endopeptidase; Penicillin-binding protein 4; PBP-4 [H]

Number of amino acids: Translated: 479; Mature: 479

Protein sequence:

>479_residues
MKKLSSISTALGSFLLSVSFSLPTFANINVSDLTQKLPEGSNAGVIAKNINQNQIIADYNGSTFMLPASTQKVFTAVAAK
LALGDQFQFETALLSNGKIQNGNLDGNLIVSFTGDPDLTRGQLYSLLAELKKQGIKKINGDLVLDTSVFSSHDRGLGWIW
NDLTMCFNSPPAAANIDNNCFYAELDANKNPGEIVKINVPAQFPIQVFGQVYVADSNEAPYCQLDVVVHDNNRYQVKGCL
ARQYKPFGLSFAVQNTDAYAAAIIQRQLRKLGIEFNGKVLLPQKPQQGQLLAKHLSKPLPDLLKKMMKKSDNQIADSLFR
AVAFNYYKRPASFQLGTLAVKSILQKQGIRFGNSILADGSGLSRHNLVAPKTMLSVLEYIAKNEDKLHLMETFPIAGVDG
TISGRGGLISPPLVKNVIAKTGSLKGVYNLAGFMTNARGEKVAFVQFINGYSTGDLESKTKRAPLVQFERNLYNELYKY

Sequences:

>Translated_479_residues
MKKLSSISTALGSFLLSVSFSLPTFANINVSDLTQKLPEGSNAGVIAKNINQNQIIADYNGSTFMLPASTQKVFTAVAAK
LALGDQFQFETALLSNGKIQNGNLDGNLIVSFTGDPDLTRGQLYSLLAELKKQGIKKINGDLVLDTSVFSSHDRGLGWIW
NDLTMCFNSPPAAANIDNNCFYAELDANKNPGEIVKINVPAQFPIQVFGQVYVADSNEAPYCQLDVVVHDNNRYQVKGCL
ARQYKPFGLSFAVQNTDAYAAAIIQRQLRKLGIEFNGKVLLPQKPQQGQLLAKHLSKPLPDLLKKMMKKSDNQIADSLFR
AVAFNYYKRPASFQLGTLAVKSILQKQGIRFGNSILADGSGLSRHNLVAPKTMLSVLEYIAKNEDKLHLMETFPIAGVDG
TISGRGGLISPPLVKNVIAKTGSLKGVYNLAGFMTNARGEKVAFVQFINGYSTGDLESKTKRAPLVQFERNLYNELYKY
>Mature_479_residues
MKKLSSISTALGSFLLSVSFSLPTFANINVSDLTQKLPEGSNAGVIAKNINQNQIIADYNGSTFMLPASTQKVFTAVAAK
LALGDQFQFETALLSNGKIQNGNLDGNLIVSFTGDPDLTRGQLYSLLAELKKQGIKKINGDLVLDTSVFSSHDRGLGWIW
NDLTMCFNSPPAAANIDNNCFYAELDANKNPGEIVKINVPAQFPIQVFGQVYVADSNEAPYCQLDVVVHDNNRYQVKGCL
ARQYKPFGLSFAVQNTDAYAAAIIQRQLRKLGIEFNGKVLLPQKPQQGQLLAKHLSKPLPDLLKKMMKKSDNQIADSLFR
AVAFNYYKRPASFQLGTLAVKSILQKQGIRFGNSILADGSGLSRHNLVAPKTMLSVLEYIAKNEDKLHLMETFPIAGVDG
TISGRGGLISPPLVKNVIAKTGSLKGVYNLAGFMTNARGEKVAFVQFINGYSTGDLESKTKRAPLVQFERNLYNELYKY

Specific function: Not involved in transpeptidation but exclusively catalyzes a DD-carboxypeptidase and DD-endopeptidase reaction [H]

COG id: COG2027

COG function: function code M; D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 4)

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S13 family [H]

Homologues:

Organism=Escherichia coli, GI1789573, Length=454, Percent_Identity=44.273127753304, Blast_Score=419, Evalue=1e-118,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR000667 [H]

Pfam domain/function: PF02113 Peptidase_S13 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 52435; Mature: 52435

Theoretical pI: Translated: 9.83; Mature: 9.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLSSISTALGSFLLSVSFSLPTFANINVSDLTQKLPEGSNAGVIAKNINQNQIIADYN
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCCCCEEEECCCCCEEEEECC
GSTFMLPASTQKVFTAVAAKLALGDQFQFETALLSNGKIQNGNLDGNLIVSFTGDPDLTR
CCEEEEECCHHHHHHHHHHHHHCCCCCEEHHHHHCCCEEECCCCCCEEEEEECCCCCCCH
GQLYSLLAELKKQGIKKINGDLVLDTSVFSSHDRGLGWIWNDLTMCFNSPPAAANIDNNC
HHHHHHHHHHHHHHHHHCCCCEEEEEHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCE
FYAELDANKNPGEIVKINVPAQFPIQVFGQVYVADSNEAPYCQLDVVVHDNNRYQVKGCL
EEEEECCCCCCCCEEEEECCCCCCCEEEEEEEEECCCCCCEEEEEEEEECCCEEEEEEHH
ARQYKPFGLSFAVQNTDAYAAAIIQRQLRKLGIEFNGKVLLPQKPQQGQLLAKHLSKPLP
HHCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCEEECCCCCCCHHHHHHHHCCCHH
DLLKKMMKKSDNQIADSLFRAVAFNYYKRPASFQLGTLAVKSILQKQGIRFGNSILADGS
HHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCEEECCC
GLSRHNLVAPKTMLSVLEYIAKNEDKLHLMETFPIAGVDGTISGRGGLISPPLVKNVIAK
CCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHH
TGSLKGVYNLAGFMTNARGEKVAFVQFINGYSTGDLESKTKRAPLVQFERNLYNELYKY
CCCCCHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCHHHCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKLSSISTALGSFLLSVSFSLPTFANINVSDLTQKLPEGSNAGVIAKNINQNQIIADYN
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCCCCEEEECCCCCEEEEECC
GSTFMLPASTQKVFTAVAAKLALGDQFQFETALLSNGKIQNGNLDGNLIVSFTGDPDLTR
CCEEEEECCHHHHHHHHHHHHHCCCCCEEHHHHHCCCEEECCCCCCEEEEEECCCCCCCH
GQLYSLLAELKKQGIKKINGDLVLDTSVFSSHDRGLGWIWNDLTMCFNSPPAAANIDNNC
HHHHHHHHHHHHHHHHHCCCCEEEEEHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCE
FYAELDANKNPGEIVKINVPAQFPIQVFGQVYVADSNEAPYCQLDVVVHDNNRYQVKGCL
EEEEECCCCCCCCEEEEECCCCCCCEEEEEEEEECCCCCCEEEEEEEEECCCEEEEEEHH
ARQYKPFGLSFAVQNTDAYAAAIIQRQLRKLGIEFNGKVLLPQKPQQGQLLAKHLSKPLP
HHCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCEEECCCCCCCHHHHHHHHCCCHH
DLLKKMMKKSDNQIADSLFRAVAFNYYKRPASFQLGTLAVKSILQKQGIRFGNSILADGS
HHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCEEECCC
GLSRHNLVAPKTMLSVLEYIAKNEDKLHLMETFPIAGVDGTISGRGGLISPPLVKNVIAK
CCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHH
TGSLKGVYNLAGFMTNARGEKVAFVQFINGYSTGDLESKTKRAPLVQFERNLYNELYKY
CCCCCHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCHHHCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]