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The map label for this gene is ispA [H]

Identifier: 148825981

GI number: 148825981

Start: 981151

End: 982041

Strand: Direct

Name: ispA [H]

Synonym: CGSHiEE_04790

Alternate gene names: 148825981

Gene position: 981151-982041 (Clockwise)

Preceding gene: 148825980

Following gene: 148825982

Centisome position: 54.12

GC content: 38.27

Gene sequence:

>891_bases
ATGGGTAACTTTTCTGAAGAATTACAACAGGTTCAAATTCGTATCAATCGCTTTTTAGAAGCTCAATTTGATGGCATTGA
AAGTCATAATGCGCCTTTGCTTGAGGCGATGAAATACGCATTATTACTTGGCGGTAAGCGAGTTCGCCCTTTCTTAGTTT
ACGCAACAGGTCAAATGCTTGGTGCAGAGAAACAAACCTTAGATTACGCTGCTGCTGCCATTGAAGCCATTCACGCCTAT
TCCTTAATTCACGATGATTTACCTGCAATGGATGATGACAATCTACGTCGTGGACATCCTACTTGTCATATCCAATTTGA
TGAAGCTACAGCTATTCTTGCGGGCGATGCGCTGCAAAGTTTCGCATTTGAAATATTAACCAAAACACCGAATATTTCTG
CTGAACAAAAACTGGCTTTAATTCAAATTTTAGCGCAAGGCGCTGGTGTGCAAGGAATGTGTTTAGGGCAAAGTTTAGAT
CTTATTTCTGAGCATAAACAGATTAGTTTAAGCGAATTAGAATTAATTCATCGTAACAAAACGGGTGCATTGCTAATTGC
CGCATTGAAATTAGGTTTCATTTGTTCTCCGCATTTTACCGACAAAAGATTAGAACAATCCTTAACACAATATGCGGAAG
CCATTGGTTTAGCCTTTCAAGTTCAAGACGATATTTTAGATATTGAAGGCGATAGTGCAGAAATTGGCAAACAGGTGGGC
GCCGATCTTGATTTAGATAAAAGTACATATCCAAAATTACTTGGATTAAGTGGCGCAAAACAAAAAGCGCAAGATCTATA
TCAAAGTGCTTTGTCTGAATTAGAAAAAATTCCTTTTGATACAACCGCACTTCGTGCATTAGCTGAATTCGTTATTACCA
GAAAAAGTTAA

Upstream 100 bases:

>100_bases
GTTTACAACAAGCAGAACAACGTATTCAGATCTTATTGCAAAAAACAGAAGACGCGCCACTGAATGATTATAAAGGGAAT
GATTATGAAGGGAATGCTTA

Downstream 100 bases:

>100_bases
ACACTCACAAAAGTGCCGTCAAAATTTATCACGTTTTTAAAATGACTAATAATATGAACAATTATCCTCTTTTATCTTTA
ATTAATTCTCCAGAAGATTT

Product: exodeoxyribonuclease VII small subunit

Products: NA

Alternate protein names: FPP synthase; (2E,6E)-farnesyl diphosphate synthase; Geranyltranstransferase [H]

Number of amino acids: Translated: 296; Mature: 295

Protein sequence:

>296_residues
MGNFSEELQQVQIRINRFLEAQFDGIESHNAPLLEAMKYALLLGGKRVRPFLVYATGQMLGAEKQTLDYAAAAIEAIHAY
SLIHDDLPAMDDDNLRRGHPTCHIQFDEATAILAGDALQSFAFEILTKTPNISAEQKLALIQILAQGAGVQGMCLGQSLD
LISEHKQISLSELELIHRNKTGALLIAALKLGFICSPHFTDKRLEQSLTQYAEAIGLAFQVQDDILDIEGDSAEIGKQVG
ADLDLDKSTYPKLLGLSGAKQKAQDLYQSALSELEKIPFDTTALRALAEFVITRKS

Sequences:

>Translated_296_residues
MGNFSEELQQVQIRINRFLEAQFDGIESHNAPLLEAMKYALLLGGKRVRPFLVYATGQMLGAEKQTLDYAAAAIEAIHAY
SLIHDDLPAMDDDNLRRGHPTCHIQFDEATAILAGDALQSFAFEILTKTPNISAEQKLALIQILAQGAGVQGMCLGQSLD
LISEHKQISLSELELIHRNKTGALLIAALKLGFICSPHFTDKRLEQSLTQYAEAIGLAFQVQDDILDIEGDSAEIGKQVG
ADLDLDKSTYPKLLGLSGAKQKAQDLYQSALSELEKIPFDTTALRALAEFVITRKS
>Mature_295_residues
GNFSEELQQVQIRINRFLEAQFDGIESHNAPLLEAMKYALLLGGKRVRPFLVYATGQMLGAEKQTLDYAAAAIEAIHAYS
LIHDDLPAMDDDNLRRGHPTCHIQFDEATAILAGDALQSFAFEILTKTPNISAEQKLALIQILAQGAGVQGMCLGQSLDL
ISEHKQISLSELELIHRNKTGALLIAALKLGFICSPHFTDKRLEQSLTQYAEAIGLAFQVQDDILDIEGDSAEIGKQVGA
DLDLDKSTYPKLLGLSGAKQKAQDLYQSALSELEKIPFDTTALRALAEFVITRKS

Specific function: Unknown

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=231, Percent_Identity=29.4372294372294, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1786623, Length=298, Percent_Identity=53.3557046979866, Blast_Score=276, Evalue=8e-76,
Organism=Escherichia coli, GI1789578, Length=267, Percent_Identity=29.5880149812734, Blast_Score=100, Evalue=9e-23,
Organism=Caenorhabditis elegans, GI17505681, Length=186, Percent_Identity=29.5698924731183, Blast_Score=70, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24651612, Length=219, Percent_Identity=29.6803652968037, Blast_Score=72, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.10 [H]

Molecular weight: Translated: 32423; Mature: 32292

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNFSEELQQVQIRINRFLEAQFDGIESHNAPLLEAMKYALLLGGKRVRPFLVYATGQML
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCHHH
GAEKQTLDYAAAAIEAIHAYSLIHDDLPAMDDDNLRRGHPTCHIQFDEATAILAGDALQS
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCEEEHHHHHHHH
FAFEILTKTPNISAEQKLALIQILAQGAGVQGMCLGQSLDLISEHKQISLSELELIHRNK
HHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHCCCHHHHHHHHCCC
TGALLIAALKLGFICSPHFTDKRLEQSLTQYAEAIGLAFQVQDDILDIEGDSAEIGKQVG
CCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHEEEECCEEECCCCHHHHHHHHC
ADLDLDKSTYPKLLGLSGAKQKAQDLYQSALSELEKIPFDTTALRALAEFVITRKS
CCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GNFSEELQQVQIRINRFLEAQFDGIESHNAPLLEAMKYALLLGGKRVRPFLVYATGQML
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCHHH
GAEKQTLDYAAAAIEAIHAYSLIHDDLPAMDDDNLRRGHPTCHIQFDEATAILAGDALQS
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCEEEHHHHHHHH
FAFEILTKTPNISAEQKLALIQILAQGAGVQGMCLGQSLDLISEHKQISLSELELIHRNK
HHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHCCCHHHHHHHHCCC
TGALLIAALKLGFICSPHFTDKRLEQSLTQYAEAIGLAFQVQDDILDIEGDSAEIGKQVG
CCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHEEEECCEEECCCCHHHHHHHHC
ADLDLDKSTYPKLLGLSGAKQKAQDLYQSALSELEKIPFDTTALRALAEFVITRKS
CCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]