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The map label for this gene is thrS [H]

Identifier: 148825906

GI number: 148825906

Start: 913340

End: 915271

Strand: Direct

Name: thrS [H]

Synonym: CGSHiEE_04375

Alternate gene names: 148825906

Gene position: 913340-915271 (Clockwise)

Preceding gene: 148825903

Following gene: 148825911

Centisome position: 50.38

GC content: 41.1

Gene sequence:

>1932_bases
ATGCCAATTATTACTTTACCTGACGGTTCCCAACGTCAATTCGATCATCCTGTTTCTGTTCTTGAAGTCGCACAAGATAT
TGGTGCGGGTCTTGCTAAAGCCACTATCGCAGGCCGTGTAAACGGAGAACGTCGCGATGCATGCGATGTTATCGAGCAAG
ATGCAACGTTAGAAATTATTACTGCAAAAGATGAAGACGGTTTAGAGATTATCCGCCATTCTTGCGCTCACTTGCTTGGA
CACGCTATTAAACAATTATTCCCAGATGTAAAAATGGCAATTGGCCCAACCATTGAAAATGGCTTCTATTATGATGTAGA
TTTAGATCGCTCTTTAACCCAAGAAGATATTGATGCAATCGAAAAACGTATGTTGGAATTGGCAAAAACCAATTATGACG
TCGTGAAAAAACGTGTTACTTGGCAAGAAGCGCGTGATACCTTTGAAAAACGCGGCGAGCCATACAAAATGGCAATTTTA
GATGAAAACATTGAACGTACTGCAACGCCTGCGTTATATCATCACTTAGAATATATTGATATGTGCCGTGGGCCACACGT
GCCAAATATGCGTTTCTGTCAGCATTTCAAATTACAAAAAGTCGCTGGTGCGTACTGGCGTGGCGACAGTAAAAACAAAA
TGTTACAACGTATCTATGGTACGGCTTGGGCGGATAAAAAACAGCTTGCTGAATACTTAACACGCTTAGAAGAAGCGGCA
AAACGTGACCACCGTAAAATTGGTAAAGCATTAGATTTATATCATATGCAAGAAGAAGCACCAGGTATGGTGTTCTGGCA
TAACGATGGTTGGACAATTTTCCGTGAGCTTGAAACGTTCGTGCGTACTAAATTAAAACAATACGATTATCAAGAAGTAA
AAGGCCCATTTATGATGGATCGAGTGTTGTGGGAAAAAACAGGTCACTGGCAAAATTACGCAGATTTAATGTTCACAACC
CAATCTGAAAACCGTGAATACGCAATTAAACCGATGAACTGCCCAGGACACGTTCAGATTTTTAATCAAGGTTTAAAATC
TTACCGTGATTTACCAATCCGTATGGCGGAATTTGGTTCTTGCCATCGTAATGAACCATCAGGTTCTTTACACGGTTTAA
TGCGTGTGCGTGGCTTTACTCAAGATGATGCGCATATTTTCTGTACTGAAGATCAAATTGAGAGCGAAGTAACTAGCTGT
ATCAAAATGGTGTATGACATTTATAGCACCTTTGGTTTCACTAATATTGCAGTAAAACTTTCTACTCGCCCTGAAAATCG
TATCGGTTCAGATGAAATGTGGGATCGTGCGGAAGCAGGTCTTGCAGCTGCATTAGCACATAACGGCTTGGAATATGAAA
TCCAAGAAGGCGAAGGCGCATTCTATGGCCCAAAAATTGAGTTTGCACTACGCGATTGTTTAGGTCGTGAGTGGCAATGT
GGTACAGTGCAATTAGACTTCGCGTTACCAGGTCGTTTAGATGCAACTTATGTGGCAGAAGACAATAGCCGTAAAACCCC
AGTAATGATTCATCGTGCGATTTTAGGCTCAATTGAGCGTTTTATTGGTATTATTACCGAAGAATATGCAGGTTTCTTCC
CTGCGTGGTTAGCCCCAACTCAAGCAGTTGTAATGAATATCACTGATAGCCAATCCGACTATGTACAACAAGTCGTTAAA
ACCTTATCTGATGCAGGTTTACGTGTTAAAGCAGACCTCCGAAATGAAAAAGTGGGCTTCAAAATCCGTGAACATACTTT
ACGTCGCGTGCCTTATATGCTCGTTTGTGGCGATAAAGAAATCGCAGAAGGTAAAGTGGCAGTACGTACCCGTAAAGGTG
CTGATTTAGGTACATTTACCGTGGAAGAATTTGCGGAAATCTTGAAAAATCAAGTAAGAAGCCGTGAATTAAAGTTATTG
AATGAAGAGTAA

Upstream 100 bases:

>100_bases
GGAATGAAGCCAAGTAGGGCAAAAGTGCGGTTAATTTTTATAGAATTTTTAATGAACACGTCACCTTTCGTATGGGTGAC
CACTGTATAAGGAAAAAACA

Downstream 100 bases:

>100_bases
TACTTCTCGAAAATAACCAATATAATTTACTGTTTAAACAGTGATTTCAATTTAACGGTGATCGTTTAAGCGATCACCGT
TATTTTTATCTGTTTCAGAT

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS [H]

Number of amino acids: Translated: 643; Mature: 642

Protein sequence:

>643_residues
MPIITLPDGSQRQFDHPVSVLEVAQDIGAGLAKATIAGRVNGERRDACDVIEQDATLEIITAKDEDGLEIIRHSCAHLLG
HAIKQLFPDVKMAIGPTIENGFYYDVDLDRSLTQEDIDAIEKRMLELAKTNYDVVKKRVTWQEARDTFEKRGEPYKMAIL
DENIERTATPALYHHLEYIDMCRGPHVPNMRFCQHFKLQKVAGAYWRGDSKNKMLQRIYGTAWADKKQLAEYLTRLEEAA
KRDHRKIGKALDLYHMQEEAPGMVFWHNDGWTIFRELETFVRTKLKQYDYQEVKGPFMMDRVLWEKTGHWQNYADLMFTT
QSENREYAIKPMNCPGHVQIFNQGLKSYRDLPIRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEDQIESEVTSC
IKMVYDIYSTFGFTNIAVKLSTRPENRIGSDEMWDRAEAGLAAALAHNGLEYEIQEGEGAFYGPKIEFALRDCLGREWQC
GTVQLDFALPGRLDATYVAEDNSRKTPVMIHRAILGSIERFIGIITEEYAGFFPAWLAPTQAVVMNITDSQSDYVQQVVK
TLSDAGLRVKADLRNEKVGFKIREHTLRRVPYMLVCGDKEIAEGKVAVRTRKGADLGTFTVEEFAEILKNQVRSRELKLL
NEE

Sequences:

>Translated_643_residues
MPIITLPDGSQRQFDHPVSVLEVAQDIGAGLAKATIAGRVNGERRDACDVIEQDATLEIITAKDEDGLEIIRHSCAHLLG
HAIKQLFPDVKMAIGPTIENGFYYDVDLDRSLTQEDIDAIEKRMLELAKTNYDVVKKRVTWQEARDTFEKRGEPYKMAIL
DENIERTATPALYHHLEYIDMCRGPHVPNMRFCQHFKLQKVAGAYWRGDSKNKMLQRIYGTAWADKKQLAEYLTRLEEAA
KRDHRKIGKALDLYHMQEEAPGMVFWHNDGWTIFRELETFVRTKLKQYDYQEVKGPFMMDRVLWEKTGHWQNYADLMFTT
QSENREYAIKPMNCPGHVQIFNQGLKSYRDLPIRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEDQIESEVTSC
IKMVYDIYSTFGFTNIAVKLSTRPENRIGSDEMWDRAEAGLAAALAHNGLEYEIQEGEGAFYGPKIEFALRDCLGREWQC
GTVQLDFALPGRLDATYVAEDNSRKTPVMIHRAILGSIERFIGIITEEYAGFFPAWLAPTQAVVMNITDSQSDYVQQVVK
TLSDAGLRVKADLRNEKVGFKIREHTLRRVPYMLVCGDKEIAEGKVAVRTRKGADLGTFTVEEFAEILKNQVRSRELKLL
NEE
>Mature_642_residues
PIITLPDGSQRQFDHPVSVLEVAQDIGAGLAKATIAGRVNGERRDACDVIEQDATLEIITAKDEDGLEIIRHSCAHLLGH
AIKQLFPDVKMAIGPTIENGFYYDVDLDRSLTQEDIDAIEKRMLELAKTNYDVVKKRVTWQEARDTFEKRGEPYKMAILD
ENIERTATPALYHHLEYIDMCRGPHVPNMRFCQHFKLQKVAGAYWRGDSKNKMLQRIYGTAWADKKQLAEYLTRLEEAAK
RDHRKIGKALDLYHMQEEAPGMVFWHNDGWTIFRELETFVRTKLKQYDYQEVKGPFMMDRVLWEKTGHWQNYADLMFTTQ
SENREYAIKPMNCPGHVQIFNQGLKSYRDLPIRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEDQIESEVTSCI
KMVYDIYSTFGFTNIAVKLSTRPENRIGSDEMWDRAEAGLAAALAHNGLEYEIQEGEGAFYGPKIEFALRDCLGREWQCG
TVQLDFALPGRLDATYVAEDNSRKTPVMIHRAILGSIERFIGIITEEYAGFFPAWLAPTQAVVMNITDSQSDYVQQVVKT
LSDAGLRVKADLRNEKVGFKIREHTLRRVPYMLVCGDKEIAEGKVAVRTRKGADLGTFTVEEFAEILKNQVRSRELKLLN
EE

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI38570111, Length=619, Percent_Identity=40.5492730210016, Blast_Score=474, Evalue=1e-133,
Organism=Homo sapiens, GI38202255, Length=642, Percent_Identity=39.5638629283489, Blast_Score=469, Evalue=1e-132,
Organism=Homo sapiens, GI20070344, Length=627, Percent_Identity=34.9282296650718, Blast_Score=391, Evalue=1e-108,
Organism=Escherichia coli, GI1788013, Length=642, Percent_Identity=78.816199376947, Blast_Score=1092, Evalue=0.0,
Organism=Escherichia coli, GI1786392, Length=184, Percent_Identity=26.0869565217391, Blast_Score=64, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI71984192, Length=628, Percent_Identity=38.8535031847134, Blast_Score=461, Evalue=1e-130,
Organism=Caenorhabditis elegans, GI71984184, Length=628, Percent_Identity=38.8535031847134, Blast_Score=461, Evalue=1e-130,
Organism=Saccharomyces cerevisiae, GI6322112, Length=650, Percent_Identity=36.7692307692308, Blast_Score=436, Evalue=1e-123,
Organism=Saccharomyces cerevisiae, GI6322654, Length=419, Percent_Identity=38.1861575178998, Blast_Score=303, Evalue=8e-83,
Organism=Drosophila melanogaster, GI24583839, Length=646, Percent_Identity=40.0928792569659, Blast_Score=484, Evalue=1e-137,
Organism=Drosophila melanogaster, GI24583842, Length=646, Percent_Identity=40.0928792569659, Blast_Score=484, Evalue=1e-137,
Organism=Drosophila melanogaster, GI28574141, Length=646, Percent_Identity=40.0928792569659, Blast_Score=484, Evalue=1e-137,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR012675
- InterPro:   IPR004095
- InterPro:   IPR012676
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF02824 TGS; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD [H]

EC number: =6.1.1.3 [H]

Molecular weight: Translated: 73605; Mature: 73474

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIITLPDGSQRQFDHPVSVLEVAQDIGAGLAKATIAGRVNGERRDACDVIEQDATLEII
CCEEECCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHCCCCEEEEE
TAKDEDGLEIIRHSCAHLLGHAIKQLFPDVKMAIGPTIENGFYYDVDLDRSLTQEDIDAI
EECCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCEECCCEEEEEECCCCCCHHHHHHH
EKRMLELAKTNYDVVKKRVTWQEARDTFEKRGEPYKMAILDENIERTATPALYHHLEYID
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHCCHHHHHHHHHHH
MCRGPHVPNMRFCQHFKLQKVAGAYWRGDSKNKMLQRIYGTAWADKKQLAEYLTRLEEAA
HHCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
KRDHRKIGKALDLYHMQEEAPGMVFWHNDGWTIFRELETFVRTKLKQYDYQEVKGPFMMD
HHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCHHHHCCCHHHH
RVLWEKTGHWQNYADLMFTTQSENREYAIKPMNCPGHVQIFNQGLKSYRDLPIRMAEFGS
HHHHHHCCCCCCCCCEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCEEHHHHCC
CHRNEPSGSLHGLMRVRGFTQDDAHIFCTEDQIESEVTSCIKMVYDIYSTFGFTNIAVKL
CCCCCCCHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEE
STRPENRIGSDEMWDRAEAGLAAALAHNGLEYEIQEGEGAFYGPKIEFALRDCLGREWQC
ECCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEECCCHHHHHHHHCCCCCCC
GTVQLDFALPGRLDATYVAEDNSRKTPVMIHRAILGSIERFIGIITEEYAGFFPAWLAPT
CEEEEEEECCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCC
QAVVMNITDSQSDYVQQVVKTLSDAGLRVKADLRNEKVGFKIREHTLRRVPYMLVCGDKE
EEEEEEECCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHCCEEEEECCCH
IAEGKVAVRTRKGADLGTFTVEEFAEILKNQVRSRELKLLNEE
HHCCEEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PIITLPDGSQRQFDHPVSVLEVAQDIGAGLAKATIAGRVNGERRDACDVIEQDATLEII
CEEECCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHCCCCEEEEE
TAKDEDGLEIIRHSCAHLLGHAIKQLFPDVKMAIGPTIENGFYYDVDLDRSLTQEDIDAI
EECCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCEECCCEEEEEECCCCCCHHHHHHH
EKRMLELAKTNYDVVKKRVTWQEARDTFEKRGEPYKMAILDENIERTATPALYHHLEYID
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHCCHHHHHHHHHHH
MCRGPHVPNMRFCQHFKLQKVAGAYWRGDSKNKMLQRIYGTAWADKKQLAEYLTRLEEAA
HHCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
KRDHRKIGKALDLYHMQEEAPGMVFWHNDGWTIFRELETFVRTKLKQYDYQEVKGPFMMD
HHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCHHHHCCCHHHH
RVLWEKTGHWQNYADLMFTTQSENREYAIKPMNCPGHVQIFNQGLKSYRDLPIRMAEFGS
HHHHHHCCCCCCCCCEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCEEHHHHCC
CHRNEPSGSLHGLMRVRGFTQDDAHIFCTEDQIESEVTSCIKMVYDIYSTFGFTNIAVKL
CCCCCCCHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEE
STRPENRIGSDEMWDRAEAGLAAALAHNGLEYEIQEGEGAFYGPKIEFALRDCLGREWQC
ECCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEECCCHHHHHHHHCCCCCCC
GTVQLDFALPGRLDATYVAEDNSRKTPVMIHRAILGSIERFIGIITEEYAGFFPAWLAPT
CEEEEEEECCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCC
QAVVMNITDSQSDYVQQVVKTLSDAGLRVKADLRNEKVGFKIREHTLRRVPYMLVCGDKE
EEEEEEECCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHCCEEEEECCCH
IAEGKVAVRTRKGADLGTFTVEEFAEILKNQVRSRELKLLNEE
HHCCEEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA