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The map label for this gene is wcaA [C]

Identifier: 148825760

GI number: 148825760

Start: 723971

End: 724855

Strand: Direct

Name: wcaA [C]

Synonym: CGSHiEE_03555

Alternate gene names: 148825760

Gene position: 723971-724855 (Clockwise)

Preceding gene: 148825759

Following gene: 148825761

Centisome position: 39.93

GC content: 34.24

Gene sequence:

>885_bases
ATGTTAAGCATTATTGTGCCTTCTTATAATCGAAAAGCAGAAGTGCCAGCTTTATTAGAAAGTTTAACTCAACAAACATC
GAGTAATTTTGAAGTAATTATTGTGGATGATTACTCCAAAGAGAGGGTAGTCGTTGAGCAACGCTATTCTTTTCCAGTTA
CAGTGATTCGTAACGAAACCAATCAAGGTGCAGCAGAAAGTCGCAATATTGGTGCGCGTGCATCAAAGGGAGATTGGTTG
CTTTTTCTCGATGATGATGATCGTTTTATGCCAGAAAAATCTGAAAAAATACTACAAGTGATTGAGCAAAACCCAGACAT
TAATTTTATTTATCATCCAGCTAAATGTGAAATGGTCAATGAAGGGTTTAGTTATGTGACTCAACCGATTGAACCCCAAG
AAATTTCGACAGAACGTATTTTGTTAGCCAATAAGATTGGTGGCATGCCAATGGTTGCAATTAAAAAAGAGATGTTTTTA
AAAATAGGTGGATTATCCACCGCACTTCGTTCTTTGGAAGACTATGATTTCTTGTTAAAACTGTTACAAGAGCCAAGTTT
TACCCCTTATAAAATTAATGAACCACTAACTTATTGTACTTTCCATACTAAACGCTCAAGTGTGTCTACGGATACCACTA
ATACGCAAAAAGCCATTGATTATATTCGCGAGCATTATGTGAAAACGGTAGAGCAAGCTCGTAATTTTGATATTAACGCA
AGCTATATTTTGGCTTATCCCCATATCATGAATTTATCGCGTAAAGCGGCAAAATATTATTTTGATATTTTTAAGAAAAC
AAAAAGCATTAAGCAATTTATTATTACTTTGGTTATTTTAATTTCGCCAAAATTAGCCATTAATTTAAAACGGTTGGGAA
AATAA

Upstream 100 bases:

>100_bases
GTGGATCTTTATCCAATAATTTACTTAAAAAATTAATGAAATATCCATTGAAAAAAATCTTTGCGATTAAGAAAAATTTG
GCTAATTAAAGGAAGGAATT

Downstream 100 bases:

>100_bases
AATGAAATTTTCAGTCCTAATGTCCTTATATATTAAGGAAAATCCTCAATTCTTGCGGGAGTGTTTTGAAAGTCTTGTTG
CACAAACTCGTCAAGCAGAT

Product: putative UDP-galactose--lipooligosaccharide galactosyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MLSIIVPSYNRKAEVPALLESLTQQTSSNFEVIIVDDYSKERVVVEQRYSFPVTVIRNETNQGAAESRNIGARASKGDWL
LFLDDDDRFMPEKSEKILQVIEQNPDINFIYHPAKCEMVNEGFSYVTQPIEPQEISTERILLANKIGGMPMVAIKKEMFL
KIGGLSTALRSLEDYDFLLKLLQEPSFTPYKINEPLTYCTFHTKRSSVSTDTTNTQKAIDYIREHYVKTVEQARNFDINA
SYILAYPHIMNLSRKAAKYYFDIFKKTKSIKQFIITLVILISPKLAINLKRLGK

Sequences:

>Translated_294_residues
MLSIIVPSYNRKAEVPALLESLTQQTSSNFEVIIVDDYSKERVVVEQRYSFPVTVIRNETNQGAAESRNIGARASKGDWL
LFLDDDDRFMPEKSEKILQVIEQNPDINFIYHPAKCEMVNEGFSYVTQPIEPQEISTERILLANKIGGMPMVAIKKEMFL
KIGGLSTALRSLEDYDFLLKLLQEPSFTPYKINEPLTYCTFHTKRSSVSTDTTNTQKAIDYIREHYVKTVEQARNFDINA
SYILAYPHIMNLSRKAAKYYFDIFKKTKSIKQFIITLVILISPKLAINLKRLGK
>Mature_294_residues
MLSIIVPSYNRKAEVPALLESLTQQTSSNFEVIIVDDYSKERVVVEQRYSFPVTVIRNETNQGAAESRNIGARASKGDWL
LFLDDDDRFMPEKSEKILQVIEQNPDINFIYHPAKCEMVNEGFSYVTQPIEPQEISTERILLANKIGGMPMVAIKKEMFL
KIGGLSTALRSLEDYDFLLKLLQEPSFTPYKINEPLTYCTFHTKRSSVSTDTTNTQKAIDYIREHYVKTVEQARNFDINA
SYILAYPHIMNLSRKAAKYYFDIFKKTKSIKQFIITLVILISPKLAINLKRLGK

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG0463

COG function: function code M; Glycosyltransferases involved in cell wall biogenesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1788372, Length=201, Percent_Identity=26.865671641791, Blast_Score=72, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001173 [H]

Pfam domain/function: PF00535 Glycos_transf_2 [H]

EC number: NA

Molecular weight: Translated: 33783; Mature: 33783

Theoretical pI: Translated: 8.84; Mature: 8.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSIIVPSYNRKAEVPALLESLTQQTSSNFEVIIVDDYSKERVVVEQRYSFPVTVIRNET
CEEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEECCCCCEEEEECCC
NQGAAESRNIGARASKGDWLLFLDDDDRFMPEKSEKILQVIEQNPDINFIYHPAKCEMVN
CCCCCHHCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEEECCHHHHHHH
EGFSYVTQPIEPQEISTERILLANKIGGMPMVAIKKEMFLKIGGLSTALRSLEDYDFLLK
CCHHHHCCCCCCCCCCCCCEEEEHHCCCCCHHHHHHHHHHEECCHHHHHHHHHHHHHHHH
LLQEPSFTPYKINEPLTYCTFHTKRSSVSTDTTNTQKAIDYIREHYVKTVEQARNFDINA
HHHCCCCCCEECCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
SYILAYPHIMNLSRKAAKYYFDIFKKTKSIKQFIITLVILISPKLAINLKRLGK
EEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHCCC
>Mature Secondary Structure
MLSIIVPSYNRKAEVPALLESLTQQTSSNFEVIIVDDYSKERVVVEQRYSFPVTVIRNET
CEEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEECCCCCEEEEECCC
NQGAAESRNIGARASKGDWLLFLDDDDRFMPEKSEKILQVIEQNPDINFIYHPAKCEMVN
CCCCCHHCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEEECCHHHHHHH
EGFSYVTQPIEPQEISTERILLANKIGGMPMVAIKKEMFLKIGGLSTALRSLEDYDFLLK
CCHHHHCCCCCCCCCCCCCEEEEHHCCCCCHHHHHHHHHHEECCHHHHHHHHHHHHHHHH
LLQEPSFTPYKINEPLTYCTFHTKRSSVSTDTTNTQKAIDYIREHYVKTVEQARNFDINA
HHHCCCCCCEECCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
SYILAYPHIMNLSRKAAKYYFDIFKKTKSIKQFIITLVILISPKLAINLKRLGK
EEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]