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The map label for this gene is metE

Identifier: 148825755

GI number: 148825755

Start: 716623

End: 718893

Strand: Direct

Name: metE

Synonym: CGSHiEE_03520

Alternate gene names: 148825755

Gene position: 716623-718893 (Clockwise)

Preceding gene: 148825754

Following gene: 148825757

Centisome position: 39.53

GC content: 40.07

Gene sequence:

>2271_bases
ATGACAACATCACATATTTTAGGCTTTCCTCGTGTAGGGGCAAAACGTGAATTAAAATTTGCACAAGAACGTTATTGGCG
TAAAGAATTAGCAGAGCAAGATTTATTAGATTTAGCGAAAGCATTGCGTGAAAAAAACTGGAAACATCAAGCGGCTGCCA
ATGCGGATTTCGTTGCAGTGGGCGATTTCACGTTCTACGATCATATTTTAGATTTACAAGTGGCAACAGGGGCAATTCCT
GCTCGTTTTGGTTTTGATAGCCAAAATTTAACCCTTGATCAATATTTCCAACTTGCACGTGGTAACAAAGATCAATTCGC
AATTGAAATGACCAAATGGTTTGATACGAACTATCACTATCTTGTGCCTGAGTTCCAAAAATCGACCGCTTTCAAAGCTA
ATCCAGCACATTATGTAAACCAAATCCGTGAAGCAAAAGCCTTAGGCTTAAACTTCAAACCTGTGATTGTTGGCCCATTA
ACATTCTTATGGTTAGGTAAAGAAAAAGGCGAAGCATTTAACCGTTTCGATTTATTAAATCAATTAGTGCCTGTTTATGT
TGAAATTTTAAATGCATTAGTGGCTGAAGGGGCAGAATGGATTCAAATTGATGAACCTGCATTAGCATTAGATTTACCAG
CAGAATGGGTTGAAGCCTATAAATCTGTTTACGCTGAATTAAGCAAAGTGAACGCAAAATTATTATTAGCCACTTATTTT
GGTTCAGTTGCAGAACACGCTGAATTATTAAAAGCCTTACCTGTTGCAGGCTTGCATTTAGATTTAGTTCGTGCACCAGA
ACAACTTGCGGCATTTGAAGATTACAGCAAAGTGTTATCAGCTGGTGTGATTGAAGGTCGTAATATCTGGCGTGCAAACT
TAAACAAAGTGTTAGATGTATTAGAGCCATTAAAAGCAAAATTAGGTGAGCGTTTATGGATTGCACCAAGCTGTTCATTA
TTGCATACCCCATTTGATTTAGAAGTGGAAGTACAATTAAAAGAAAAAAATACCGCACTTTACAGCTGGTTATCTTTCAC
GCTACAAAAAGTAGAAGAATTAAACGTATTAAAACAAGCGTTAAATAATGGCAGAGCGTCTGTACAAGCAGCATTAGATG
CAAGCCAAGCGGCAGCAGATGCACGTGCAACCTCAAAAGAAATTCATCGCCCTGAAGTGGCAGAACGTTTAGCAAACTTG
CCAAAAGGTGCGGATCAACGTAAATCGCCATTTGCAGAACGTATCGTTAAGCAAAATGCGTGGTTAAATTTACCGCTTCT
GCCAACTACAAACATTGGTTCATTCCCACAAACTACTGAAATTCGTCACGCACGTGCAAGTTTCAAAAAAGGCGAGTTAT
CTCTTGCAGATTACGAAGCGGCAATGAAAAAAGAGATCGAATATGTAGTACGTCGCCAAGAAGAATTAGATTTAGATGTG
TTAGTTCACGGTGAAGCAGAACGTAACGACATGGTGGAATACTTTGGGGAATTATTAGATGGTTTCGCATTCACTAAATT
TGGTTGGGTACAAAGCTATGGTTCACGTTGTGTAAAACCACCAGTGATTTACGGTGATGTAACTCGCCCAGAGCCAATGA
CAGTACGCTGGTCTCAATATGCACAAAGCCTCACAAACCGTGTAATGAAAGGAATGCTCACAGGCCCTGTGACTATTTTA
CAATGGTCATTCGTGCGTAACGATATTCCACGTTCAACCGTATGTAAACAAATCGGCGTAGCATTATCTGATGAAGTGTT
AGATTTAGAAGCAGCAGGCATTAAAGTCATTCAAATTGACGAACCAGCCATTCGTGAAGGTTTACCGCTTAAACGTGCAG
ATTGGGACGCATACTTACAATGGGCAGGCGAAGCATTCCGTTTAAGCTCAATGGGCGTGCAAGATGATACGCAAATTCAC
ACTCACATGTGTTATTCCGAGTTTAACGACATCTTACCTGCCATTGCGGCATTAGATGCAGACGTGATTACCATCGAAAC
TTCACGTTCAGATATGGAATTATTAACGGCATTTGCAGATTTCAAATATCCAAATGATATTGGCCCAGGTGTTTACGATA
TTCACAGCCCTCGTGTACCAACGGCGGCGGAAGTGGAACATTTATTACGTAAAGCATTAAATGTAATTCCAAAAGAACGC
TTATGGGTAAACCCAGACTGCGGTTTAAAAACGCGTGGCTGGACAGAAACCATCGACCAATTAAAAGTGATGGTGGATGT
AACCAAAAAATTACGTGCGGAATTAGCGTAA

Upstream 100 bases:

>100_bases
TAATAAGTTTGCATAATAAATGAAGATCAAGCATTCTATATTGGTATAGACGTTTAGCCGTCTAAATGATTAAAAAAATT
ACTTTAAATTGGGAGTTATT

Downstream 100 bases:

>100_bases
GTCATAATACATAAAGAATAATTTTCAAACACAACATAATTAAAGCCGAATCTCTATGGGATTCGGTTTTTCTTATAGAT
AAAATGTTGAAATATAATAA

Product: 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase

Products: NA

Alternate protein names: Cobalamin-independent methionine synthase; Methionine synthase, vitamin-B12 independent isozyme

Number of amino acids: Translated: 756; Mature: 755

Protein sequence:

>756_residues
MTTSHILGFPRVGAKRELKFAQERYWRKELAEQDLLDLAKALREKNWKHQAAANADFVAVGDFTFYDHILDLQVATGAIP
ARFGFDSQNLTLDQYFQLARGNKDQFAIEMTKWFDTNYHYLVPEFQKSTAFKANPAHYVNQIREAKALGLNFKPVIVGPL
TFLWLGKEKGEAFNRFDLLNQLVPVYVEILNALVAEGAEWIQIDEPALALDLPAEWVEAYKSVYAELSKVNAKLLLATYF
GSVAEHAELLKALPVAGLHLDLVRAPEQLAAFEDYSKVLSAGVIEGRNIWRANLNKVLDVLEPLKAKLGERLWIAPSCSL
LHTPFDLEVEVQLKEKNTALYSWLSFTLQKVEELNVLKQALNNGRASVQAALDASQAAADARATSKEIHRPEVAERLANL
PKGADQRKSPFAERIVKQNAWLNLPLLPTTNIGSFPQTTEIRHARASFKKGELSLADYEAAMKKEIEYVVRRQEELDLDV
LVHGEAERNDMVEYFGELLDGFAFTKFGWVQSYGSRCVKPPVIYGDVTRPEPMTVRWSQYAQSLTNRVMKGMLTGPVTIL
QWSFVRNDIPRSTVCKQIGVALSDEVLDLEAAGIKVIQIDEPAIREGLPLKRADWDAYLQWAGEAFRLSSMGVQDDTQIH
THMCYSEFNDILPAIAALDADVITIETSRSDMELLTAFADFKYPNDIGPGVYDIHSPRVPTAAEVEHLLRKALNVIPKER
LWVNPDCGLKTRGWTETIDQLKVMVDVTKKLRAELA

Sequences:

>Translated_756_residues
MTTSHILGFPRVGAKRELKFAQERYWRKELAEQDLLDLAKALREKNWKHQAAANADFVAVGDFTFYDHILDLQVATGAIP
ARFGFDSQNLTLDQYFQLARGNKDQFAIEMTKWFDTNYHYLVPEFQKSTAFKANPAHYVNQIREAKALGLNFKPVIVGPL
TFLWLGKEKGEAFNRFDLLNQLVPVYVEILNALVAEGAEWIQIDEPALALDLPAEWVEAYKSVYAELSKVNAKLLLATYF
GSVAEHAELLKALPVAGLHLDLVRAPEQLAAFEDYSKVLSAGVIEGRNIWRANLNKVLDVLEPLKAKLGERLWIAPSCSL
LHTPFDLEVEVQLKEKNTALYSWLSFTLQKVEELNVLKQALNNGRASVQAALDASQAAADARATSKEIHRPEVAERLANL
PKGADQRKSPFAERIVKQNAWLNLPLLPTTNIGSFPQTTEIRHARASFKKGELSLADYEAAMKKEIEYVVRRQEELDLDV
LVHGEAERNDMVEYFGELLDGFAFTKFGWVQSYGSRCVKPPVIYGDVTRPEPMTVRWSQYAQSLTNRVMKGMLTGPVTIL
QWSFVRNDIPRSTVCKQIGVALSDEVLDLEAAGIKVIQIDEPAIREGLPLKRADWDAYLQWAGEAFRLSSMGVQDDTQIH
THMCYSEFNDILPAIAALDADVITIETSRSDMELLTAFADFKYPNDIGPGVYDIHSPRVPTAAEVEHLLRKALNVIPKER
LWVNPDCGLKTRGWTETIDQLKVMVDVTKKLRAELA
>Mature_755_residues
TTSHILGFPRVGAKRELKFAQERYWRKELAEQDLLDLAKALREKNWKHQAAANADFVAVGDFTFYDHILDLQVATGAIPA
RFGFDSQNLTLDQYFQLARGNKDQFAIEMTKWFDTNYHYLVPEFQKSTAFKANPAHYVNQIREAKALGLNFKPVIVGPLT
FLWLGKEKGEAFNRFDLLNQLVPVYVEILNALVAEGAEWIQIDEPALALDLPAEWVEAYKSVYAELSKVNAKLLLATYFG
SVAEHAELLKALPVAGLHLDLVRAPEQLAAFEDYSKVLSAGVIEGRNIWRANLNKVLDVLEPLKAKLGERLWIAPSCSLL
HTPFDLEVEVQLKEKNTALYSWLSFTLQKVEELNVLKQALNNGRASVQAALDASQAAADARATSKEIHRPEVAERLANLP
KGADQRKSPFAERIVKQNAWLNLPLLPTTNIGSFPQTTEIRHARASFKKGELSLADYEAAMKKEIEYVVRRQEELDLDVL
VHGEAERNDMVEYFGELLDGFAFTKFGWVQSYGSRCVKPPVIYGDVTRPEPMTVRWSQYAQSLTNRVMKGMLTGPVTILQ
WSFVRNDIPRSTVCKQIGVALSDEVLDLEAAGIKVIQIDEPAIREGLPLKRADWDAYLQWAGEAFRLSSMGVQDDTQIHT
HMCYSEFNDILPAIAALDADVITIETSRSDMELLTAFADFKYPNDIGPGVYDIHSPRVPTAAEVEHLLRKALNVIPKERL
WVNPDCGLKTRGWTETIDQLKVMVDVTKKLRAELA

Specific function: Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation

COG id: COG0620

COG function: function code E; Methionine synthase II (cobalamin-independent)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vitamin-B12 independent methionine synthase family

Homologues:

Organism=Escherichia coli, GI2367304, Length=757, Percent_Identity=54.557463672391, Blast_Score=822, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6320936, Length=775, Percent_Identity=46.3225806451613, Blast_Score=674, Evalue=0.0,

Paralogues:

None

Copy number: 45,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METE_HAEIE (A5UBH4)

Other databases:

- EMBL:   CP000671
- RefSeq:   YP_001290508.1
- ProteinModelPortal:   A5UBH4
- SMR:   A5UBH4
- STRING:   A5UBH4
- GeneID:   5225774
- GenomeReviews:   CP000671_GR
- KEGG:   hip:CGSHiEE_03520
- eggNOG:   COG0620
- HOGENOM:   HBG287495
- OMA:   RFGWVQS
- ProtClustDB:   PRK05222
- BioCyc:   HINF374930:CGSHIEE_03520-MONOMER
- HAMAP:   MF_00172
- InterPro:   IPR013215
- InterPro:   IPR006276
- InterPro:   IPR002629
- PIRSF:   PIRSF000382
- TIGRFAMs:   TIGR01371

Pfam domain/function: PF08267 Meth_synt_1; PF01717 Meth_synt_2

EC number: =2.1.1.14

Molecular weight: Translated: 85151; Mature: 85020

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTSHILGFPRVGAKRELKFAQERYWRKELAEQDLLDLAKALREKNWKHQAAANADFVAV
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCEEEE
GDFTFYDHILDLQVATGAIPARFGFDSQNLTLDQYFQLARGNKDQFAIEMTKWFDTNYHY
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEEEHHHCCCCEE
LVPEFQKSTAFKANPAHYVNQIREAKALGLNFKPVIVGPLTFLWLGKEKGEAFNRFDLLN
ECCCHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEECCEEEEEECCCCCCHHHHHHHHH
QLVPVYVEILNALVAEGAEWIQIDEPALALDLPAEWVEAYKSVYAELSKVNAKLLLATYF
HHHHHHHHHHHHHHHCCCCEEEECCCCEEEECCHHHHHHHHHHHHHHHHCCHHHHHHHHH
GSVAEHAELLKALPVAGLHLDLVRAPEQLAAFEDYSKVLSAGVIEGRNIWRANLNKVLDV
HHHHHHHHHHHHCCCCCCCHHHHHCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
LEPLKAKLGERLWIAPSCSLLHTPFDLEVEVQLKEKNTALYSWLSFTLQKVEELNVLKQA
HHHHHHHHCCEEEECCCCCEEECCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
LNNGRASVQAALDASQAAADARATSKEIHRPEVAERLANLPKGADQRKSPFAERIVKQNA
HCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHCCHHHHHHHHCCC
WLNLPLLPTTNIGSFPQTTEIRHARASFKKGELSLADYEAAMKKEIEYVVRRQEELDLDV
CEECCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEE
LVHGEAERNDMVEYFGELLDGFAFTKFGWVQSYGSRCVKPPVIYGDVTRPEPMTVRWSQY
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCEEEHHHH
AQSLTNRVMKGMLTGPVTILQWSFVRNDIPRSTVCKQIGVALSDEVLDLEAAGIKVIQID
HHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHCCHHCCHHHEEECCCEEEEEEC
EPAIREGLPLKRADWDAYLQWAGEAFRLSSMGVQDDTQIHTHMCYSEFNDILPAIAALDA
CHHHHCCCCCCCCCHHHHHHHCCCHHEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
DVITIETSRSDMELLTAFADFKYPNDIGPGVYDIHSPRVPTAAEVEHLLRKALNVIPKER
CEEEEECCCHHHHHHHHHHHCCCCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHCCCCC
LWVNPDCGLKTRGWTETIDQLKVMVDVTKKLRAELA
EEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TTSHILGFPRVGAKRELKFAQERYWRKELAEQDLLDLAKALREKNWKHQAAANADFVAV
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCEEEE
GDFTFYDHILDLQVATGAIPARFGFDSQNLTLDQYFQLARGNKDQFAIEMTKWFDTNYHY
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEEEHHHCCCCEE
LVPEFQKSTAFKANPAHYVNQIREAKALGLNFKPVIVGPLTFLWLGKEKGEAFNRFDLLN
ECCCHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEECCEEEEEECCCCCCHHHHHHHHH
QLVPVYVEILNALVAEGAEWIQIDEPALALDLPAEWVEAYKSVYAELSKVNAKLLLATYF
HHHHHHHHHHHHHHHCCCCEEEECCCCEEEECCHHHHHHHHHHHHHHHHCCHHHHHHHHH
GSVAEHAELLKALPVAGLHLDLVRAPEQLAAFEDYSKVLSAGVIEGRNIWRANLNKVLDV
HHHHHHHHHHHHCCCCCCCHHHHHCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
LEPLKAKLGERLWIAPSCSLLHTPFDLEVEVQLKEKNTALYSWLSFTLQKVEELNVLKQA
HHHHHHHHCCEEEECCCCCEEECCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
LNNGRASVQAALDASQAAADARATSKEIHRPEVAERLANLPKGADQRKSPFAERIVKQNA
HCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHCCHHHHHHHHCCC
WLNLPLLPTTNIGSFPQTTEIRHARASFKKGELSLADYEAAMKKEIEYVVRRQEELDLDV
CEECCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEE
LVHGEAERNDMVEYFGELLDGFAFTKFGWVQSYGSRCVKPPVIYGDVTRPEPMTVRWSQY
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCEEEHHHH
AQSLTNRVMKGMLTGPVTILQWSFVRNDIPRSTVCKQIGVALSDEVLDLEAAGIKVIQID
HHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHCCHHCCHHHEEECCCEEEEEEC
EPAIREGLPLKRADWDAYLQWAGEAFRLSSMGVQDDTQIHTHMCYSEFNDILPAIAALDA
CHHHHCCCCCCCCCHHHHHHHCCCHHEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
DVITIETSRSDMELLTAFADFKYPNDIGPGVYDIHSPRVPTAAEVEHLLRKALNVIPKER
CEEEEECCCHHHHHHHHHHHCCCCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHCCCCC
LWVNPDCGLKTRGWTETIDQLKVMVDVTKKLRAELA
EEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA