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The map label for this gene is metR [H]

Identifier: 148825725

GI number: 148825725

Start: 681272

End: 682201

Strand: Direct

Name: metR [H]

Synonym: CGSHiEE_03350

Alternate gene names: 148825725

Gene position: 681272-682201 (Clockwise)

Preceding gene: 148825724

Following gene: 148825726

Centisome position: 37.58

GC content: 38.49

Gene sequence:

>930_bases
ATGAAACCAACCTTTCTAGAATTTAGACATCTTAAAACTTTATTGGCTTTAAAAGAGACAGGCAGCGTTTCTCTAGCTGC
AAAGCGCGTATATCTAACCCAATCAGCACTTTCTCACCAAATTAAATTAATAGAAGAACAATTTGGCTTGCCTTTATTTG
AGCGTAAAAGTAATCCACTTCGTTTTACTTCAGCGGGAGAGCGTTTAATTCGTCTTGCGAATGAAGTAATACCGAAACTG
ATTGATGCTGAACGTGATCTTGCACGGGTAAAACACGGCGATGCAGGACAGCTACGTATAGCGGTGGAATGCCATACTTG
TTTTGATTGGTTAATGCCAGCGATGGATGAATTCCGTCAGCATTGGGGGCTAGTAGAACTGGATATTGTATCTGGCTTTC
ATACTGATCCCGTTGGTTTATTACTTTCCCATAGAGCAGATTGGGCGATAGTATCAGAAATAGAGCATAATGATGATGTC
ATTTTTAAACCGCTCTTTTCTTATGAAATGGTTGGGATTTGCTCAAAAAATCATTCTTTAGTAGAAAAAGATATTTGGGA
AGCGGAAGATTTTATTGATGAAACTTGGGTAACTTACCCCGTACCTGACGATATGTTAGATCTATGGCGTAAAGTGCTTA
AATCAAAAGGCATTAATCCTACTCGCCGTACAACAGAACTTACTATTGCAATGATTCAACTTGTTGCGAGCAAACGCGGC
ATTGCCACAGTGCCTTATTGGGCTGCCTTGCCTTATTTGGAAAAAGGTTATGTTGTGGCAAGAAAAATCACAAAAAATGG
CTTATACAGCAACTTATATGCCGCGATTCGCAAAGAAGATGAATCCCTTTCTTATTTAGAAGATTTTTATCAAACAGTCA
AATCTCAAAGTTTCTCAACTTTACCCGGACTTTCTGTTTTAAATTTATAG

Upstream 100 bases:

>100_bases
AAAATAATTATCTAAATAATTAATTTTATTAATTAGCATATTTCTAATTTTAATGTATTATACTGGTTCCCTAATTCTTA
ATTTTATATGAATATTATTA

Downstream 100 bases:

>100_bases
ATATAATTAATGAATTTATCTCAACAAAATCAACAGCCTAACCCAATTACTGAAGCGGCTAAAGCTGCTTTTCCCTATAG
CGTACCAATGATTGCAGGAT

Product: transcriptional activator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MKPTFLEFRHLKTLLALKETGSVSLAAKRVYLTQSALSHQIKLIEEQFGLPLFERKSNPLRFTSAGERLIRLANEVIPKL
IDAERDLARVKHGDAGQLRIAVECHTCFDWLMPAMDEFRQHWGLVELDIVSGFHTDPVGLLLSHRADWAIVSEIEHNDDV
IFKPLFSYEMVGICSKNHSLVEKDIWEAEDFIDETWVTYPVPDDMLDLWRKVLKSKGINPTRRTTELTIAMIQLVASKRG
IATVPYWAALPYLEKGYVVARKITKNGLYSNLYAAIRKEDESLSYLEDFYQTVKSQSFSTLPGLSVLNL

Sequences:

>Translated_309_residues
MKPTFLEFRHLKTLLALKETGSVSLAAKRVYLTQSALSHQIKLIEEQFGLPLFERKSNPLRFTSAGERLIRLANEVIPKL
IDAERDLARVKHGDAGQLRIAVECHTCFDWLMPAMDEFRQHWGLVELDIVSGFHTDPVGLLLSHRADWAIVSEIEHNDDV
IFKPLFSYEMVGICSKNHSLVEKDIWEAEDFIDETWVTYPVPDDMLDLWRKVLKSKGINPTRRTTELTIAMIQLVASKRG
IATVPYWAALPYLEKGYVVARKITKNGLYSNLYAAIRKEDESLSYLEDFYQTVKSQSFSTLPGLSVLNL
>Mature_309_residues
MKPTFLEFRHLKTLLALKETGSVSLAAKRVYLTQSALSHQIKLIEEQFGLPLFERKSNPLRFTSAGERLIRLANEVIPKL
IDAERDLARVKHGDAGQLRIAVECHTCFDWLMPAMDEFRQHWGLVELDIVSGFHTDPVGLLLSHRADWAIVSEIEHNDDV
IFKPLFSYEMVGICSKNHSLVEKDIWEAEDFIDETWVTYPVPDDMLDLWRKVLKSKGINPTRRTTELTIAMIQLVASKRG
IATVPYWAALPYLEKGYVVARKITKNGLYSNLYAAIRKEDESLSYLEDFYQTVKSQSFSTLPGLSVLNL

Specific function: Control of the last step in methionine biosynthesis; metR is a positive activator of the metA, metE and metH genes [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790262, Length=298, Percent_Identity=37.9194630872483, Blast_Score=223, Evalue=1e-59,
Organism=Escherichia coli, GI145693105, Length=186, Percent_Identity=26.3440860215054, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI157672245, Length=189, Percent_Identity=27.5132275132275, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1790208, Length=250, Percent_Identity=28, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1786401, Length=158, Percent_Identity=27.2151898734177, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787530, Length=183, Percent_Identity=28.9617486338798, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87082024, Length=288, Percent_Identity=20.8333333333333, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35326; Mature: 35326

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS50931 HTH_LYSR ; PS00300 SRP54

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPTFLEFRHLKTLLALKETGSVSLAAKRVYLTQSALSHQIKLIEEQFGLPLFERKSNPL
CCCCHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCE
RFTSAGERLIRLANEVIPKLIDAERDLARVKHGDAGQLRIAVECHTCFDWLMPAMDEFRQ
EECHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHHHHHH
HWGLVELDIVSGFHTDPVGLLLSHRADWAIVSEIEHNDDVIFKPLFSYEMVGICSKNHSL
HCCEEEEEEECCCCCCCHHHHHHCCCCEEEEECCCCCCCEEEEHHHHHHHHHHCCCCCHH
VEKDIWEAEDFIDETWVTYPVPDDMLDLWRKVLKSKGINPTRRTTELTIAMIQLVASKRG
HHHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCC
IATVPYWAALPYLEKGYVVARKITKNGLYSNLYAAIRKEDESLSYLEDFYQTVKSQSFST
CEECCHHHHCCHHHCCCCEEHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCC
LPGLSVLNL
CCCCHHCCC
>Mature Secondary Structure
MKPTFLEFRHLKTLLALKETGSVSLAAKRVYLTQSALSHQIKLIEEQFGLPLFERKSNPL
CCCCHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCE
RFTSAGERLIRLANEVIPKLIDAERDLARVKHGDAGQLRIAVECHTCFDWLMPAMDEFRQ
EECHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHHHHHH
HWGLVELDIVSGFHTDPVGLLLSHRADWAIVSEIEHNDDVIFKPLFSYEMVGICSKNHSL
HCCEEEEEEECCCCCCCHHHHHHCCCCEEEEECCCCCCCEEEEHHHHHHHHHHCCCCCHH
VEKDIWEAEDFIDETWVTYPVPDDMLDLWRKVLKSKGINPTRRTTELTIAMIQLVASKRG
HHHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCC
IATVPYWAALPYLEKGYVVARKITKNGLYSNLYAAIRKEDESLSYLEDFYQTVKSQSFST
CEECCHHHHCCHHHCCCCEEHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCC
LPGLSVLNL
CCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]