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The map label for this gene is xthA [H]

Identifier: 148825679

GI number: 148825679

Start: 622838

End: 623641

Strand: Direct

Name: xthA [H]

Synonym: CGSHiEE_03095

Alternate gene names: 148825679

Gene position: 622838-623641 (Clockwise)

Preceding gene: 148825678

Following gene: 148825680

Centisome position: 34.35

GC content: 36.94

Gene sequence:

>804_bases
ATGAAATTTATCTCTTTTAATATAAATGGTTTACGTGCTAGACCACATCAACTTGAAGCCATTATTGAAAAATATCAGCC
AGATGTGATTGGCTTGCAGGAAATTAAAGTTGCTGACGAGGCTTTTCCCTATGAAATTACAGAAAACTTAGGTTATCACG
TATTTCATCACGGACAGAAAGGTCATTATGGTGTGGCATTATTGACTAAACAAGAACCTAAAGCGGTTCGTCGTGGTTTT
TCAACTGACAATGAAGATGCGCAAAAACGCATTATTATGGCTGATCTTGAAACTGAATTTGGCTTATTGACTGTCATTAA
TGGTTATTTTCCTCAAGGGGAAAGTCGTACGCACGAGACTAAATTTCCAGCTAAAGAGAAATTTTATGCAGATCTTCAAC
AGTATTTAGAAAAAGAACACGACAAATCTAATCCGATTTTAATTATGGGCGATATGAATATTAGCCCAAGTGATTTAGAT
ATTGGTATCGGCGATGAAAATCGTAAACGTTGGTTACGCACAGGTAAATGCTCTTTCTTACCTGAAGAACGAGCGTGGTA
TCAACGTTTGTATGATTATGGATTAGAAGACAGTTTCCGTAAGCTGAACCCAACAGCAAATGATAAATTCTCGTGGTTTG
ATTACCGTTCTAAAGGTTTTGATGATAATCGAGGTTTACGAATTGATCATATTTTAGTAAGCCAAAAATTGGCTGAACGC
TGTGTCGATGTGGGTATTGCATTAGATATTCGAGCAATGGAAAAACCGTCTGATCACGCACCGATTTGGGCTGAATTTAA
ATAG

Upstream 100 bases:

>100_bases
CAGTATTTGAGCAAATTCAGGTTTATCTGACAAAGCCGTTTTTATCAAAATAGAAATCTCTGTATAATAAAGCCAATTTC
ACTCTTTAATAGGAACAAAA

Downstream 100 bases:

>100_bases
GAAAGCGAAATGGATATGGCAAATTGGCAGAATTATCGTTCTACCGTGAACGGTATGCCTGCCGTGTTTACCGCAAATAT
TGAAGATGTTGAAAAATATC

Product: exonuclease III

Products: NA

Alternate protein names: EXO III; Exonuclease III [H]

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MKFISFNINGLRARPHQLEAIIEKYQPDVIGLQEIKVADEAFPYEITENLGYHVFHHGQKGHYGVALLTKQEPKAVRRGF
STDNEDAQKRIIMADLETEFGLLTVINGYFPQGESRTHETKFPAKEKFYADLQQYLEKEHDKSNPILIMGDMNISPSDLD
IGIGDENRKRWLRTGKCSFLPEERAWYQRLYDYGLEDSFRKLNPTANDKFSWFDYRSKGFDDNRGLRIDHILVSQKLAER
CVDVGIALDIRAMEKPSDHAPIWAEFK

Sequences:

>Translated_267_residues
MKFISFNINGLRARPHQLEAIIEKYQPDVIGLQEIKVADEAFPYEITENLGYHVFHHGQKGHYGVALLTKQEPKAVRRGF
STDNEDAQKRIIMADLETEFGLLTVINGYFPQGESRTHETKFPAKEKFYADLQQYLEKEHDKSNPILIMGDMNISPSDLD
IGIGDENRKRWLRTGKCSFLPEERAWYQRLYDYGLEDSFRKLNPTANDKFSWFDYRSKGFDDNRGLRIDHILVSQKLAER
CVDVGIALDIRAMEKPSDHAPIWAEFK
>Mature_267_residues
MKFISFNINGLRARPHQLEAIIEKYQPDVIGLQEIKVADEAFPYEITENLGYHVFHHGQKGHYGVALLTKQEPKAVRRGF
STDNEDAQKRIIMADLETEFGLLTVINGYFPQGESRTHETKFPAKEKFYADLQQYLEKEHDKSNPILIMGDMNISPSDLD
IGIGDENRKRWLRTGKCSFLPEERAWYQRLYDYGLEDSFRKLNPTANDKFSWFDYRSKGFDDNRGLRIDHILVSQKLAER
CVDVGIALDIRAMEKPSDHAPIWAEFK

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction [H]

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=269, Percent_Identity=27.5092936802974, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI18375503, Length=269, Percent_Identity=27.5092936802974, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI18375501, Length=269, Percent_Identity=27.5092936802974, Blast_Score=97, Evalue=1e-20,
Organism=Escherichia coli, GI1788046, Length=267, Percent_Identity=72.2846441947566, Blast_Score=414, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI71989536, Length=268, Percent_Identity=26.1194029850746, Blast_Score=95, Evalue=3e-20,
Organism=Drosophila melanogaster, GI221330655, Length=265, Percent_Identity=31.3207547169811, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI17136678, Length=265, Percent_Identity=31.3207547169811, Blast_Score=98, Evalue=5e-21,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 31003; Mature: 31003

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS00726 AP_NUCLEASE_F1_1 ; PS00727 AP_NUCLEASE_F1_2 ; PS00728 AP_NUCLEASE_F1_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFISFNINGLRARPHQLEAIIEKYQPDVIGLQEIKVADEAFPYEITENLGYHVFHHGQK
CEEEEECCCCCCCCHHHHHHHHHHHCCCEECHHHEEECCCCCCCHHHHCCCEEEEECCCC
GHYGVALLTKQEPKAVRRGFSTDNEDAQKRIIMADLETEFGLLTVINGYFPQGESRTHET
CCEEEEEEECCCCHHHHCCCCCCCCHHHHEEEEEECCCCCCEEEEEECCCCCCCCCCCCC
KFPAKEKFYADLQQYLEKEHDKSNPILIMGDMNISPSDLDIGIGDENRKRWLRTGKCSFL
CCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEECCCCCHHHHHHHCCCCCCC
PEERAWYQRLYDYGLEDSFRKLNPTANDKFSWFDYRSKGFDDNRGLRIDHILVSQKLAER
CHHHHHHHHHHHCCCCCHHHHCCCCCCCCCEEEECCCCCCCCCCCEEEHHHHHHHHHHHH
CVDVGIALDIRAMEKPSDHAPIWAEFK
HHHCCEEEEEEECCCCCCCCCEEEECC
>Mature Secondary Structure
MKFISFNINGLRARPHQLEAIIEKYQPDVIGLQEIKVADEAFPYEITENLGYHVFHHGQK
CEEEEECCCCCCCCHHHHHHHHHHHCCCEECHHHEEECCCCCCCHHHHCCCEEEEECCCC
GHYGVALLTKQEPKAVRRGFSTDNEDAQKRIIMADLETEFGLLTVINGYFPQGESRTHET
CCEEEEEEECCCCHHHHCCCCCCCCHHHHEEEEEECCCCCCEEEEEECCCCCCCCCCCCC
KFPAKEKFYADLQQYLEKEHDKSNPILIMGDMNISPSDLDIGIGDENRKRWLRTGKCSFL
CCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEECCCCCHHHHHHHCCCCCCC
PEERAWYQRLYDYGLEDSFRKLNPTANDKFSWFDYRSKGFDDNRGLRIDHILVSQKLAER
CHHHHHHHHHHHCCCCCHHHHCCCCCCCCCEEEECCCCCCCCCCCEEEHHHHHHHHHHHH
CVDVGIALDIRAMEKPSDHAPIWAEFK
HHHCCEEEEEEECCCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]