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The map label for this gene is thrA [H]

Identifier: 148825634

GI number: 148825634

Start: 571007

End: 573454

Strand: Direct

Name: thrA [H]

Synonym: CGSHiEE_02840

Alternate gene names: 148825634

Gene position: 571007-573454 (Clockwise)

Preceding gene: 148825632

Following gene: 148825635

Centisome position: 31.49

GC content: 40.56

Gene sequence:

>2448_bases
ATGCGCGTACTTAAATTTGGCGGCACTTCGCTTGCAAATCCGGAACGTTTTTCCCAAGCGGCGAAATTGATCGAGCAAGC
TCATTTAGAAGAGCAGGCTGCAGGTGTTTTATCAGCACCAGCCAAAATAACCAACCATCTCGTCGCCCTTTCTGAAAAAG
CTGCATTAAATCAATCAACCGATACGCATTTCAACGAAGCAATCGAAATTTTCTATAACATCATTAATGGCTTACATGCC
GAAAATAATCAATTCGACTTAAATGGCACCAAGGCACTTATTGATGCTGAATTTGCTCAAATCAAAGGATTATTAGAAGA
AATTCGTCAGGCAGGAAAAGTAGAAGATGCAGTTAAAGCGACCATTGATTGCCGTGGGGAAAAACTTTCTATTGCAATGA
TGAAAGCTTGGTTTGAAGCACGTGGCTATAGCGTACATATTGTGGATCCAGTCAAGCAATTATTAGCAAAAGGCGGTTAT
CTTGAGTCTTCAGTTGAAATTGAAGAATCAACAAAACGTGTTGATGCAGCAAGTATTGCAAAAGATAAAGTGGTGCTGAT
GGCAGGTTTTACTGCGGGTAATGAAAAAGGCGAGTTGGTATTGCTCGGTCGTAATGGCTCAGACTATTCTGCAGCCTGTT
TAGCCGCTTGTTTAGGCGCAAGTGTATGCGAAATTTGGACCGATGTGGACGGCGTTTATACTTGCGATCCGCGTTTAGTG
CCTGATGCACGTTTATTACCAACGCTTTCTTATCGTGAAGCGATGGAACTCTCTTATTTCGGTGCGAAAGTGATTCATCC
TCGTACGATTGGGCCATTGTTGCCACAAAATATTCCTTGTGTCATTAAAAATACAGGAAATCCTTCTGCGCCAGGTTCGA
TTATTGATGGCAATGTGAAATCAGAAAGTTTGCAAGTTAAAGGTATCACTAACTTAGACAATTTGGCGATGTTTAATGTG
TCAGGCCCTGGTATGCAAGGTATGGTAGGCATGGCATCTCGCGTATTTTCAGCCATGTCAGGTGCAGGCATTTCAGTTAT
TTTAATTACTCAGTCTTCGTCTGAATATAGTATTAGTTTCTGTGTGCCTGTGAAATCTGCAGAAGTAGCGAAAACCGTAC
TAGAAACGGAGTTTGCTAATGAATTAAATGAACATCAATTAGAATCAATTGAAGTGATTAAAGATTTATCCATTATTTCT
GTTGTGGGCGATGGAATGAAGCAAGCGAAAGGCATTGCTGCTCGTTTCTTCTCGGCATTAGCGCAGGCAAACATTAGCAT
TGTGGCGATTGCACAAGGATCTAGCGAACGTTCAATTTCTGCGGTTGTGCCGCAAAATAAAGCCATTGAAGCGGTGAAGG
CTACCCATCAGGCGCTTTTCAATAATAAAAAAGTCGTGGATATGTTTTTAGTGGGCGTTGGCGGAGTAGGCGGAGAATTA
ATTGAGCAGGTAAAGCGTCAAAAGGAGTATCTAGCAAAGAAGAATGTTGAAATTCGAGTTTGTGCTATTGCGAATTCCAA
CCGTATGTTGCTTGATGAAAATGGATTAAATTTAGAGGATTGGAAAAACGATTTAGAAAATGCGACTCAGCCATCAGATT
TTGATGTATTGCTTTCTTTCATTAAATTACATCATGTGGTGAATCCAGTATTTGTAGATTGTACTTCAGCCGAATCTGTA
GCAGGGCTTTATGCTCGTGCGTTAAAAGAAGGTTTCCATGTGGTTACGCCAAACAAAAAAGCGAATACACGAGAATTAGT
TTATTACAATGAGTTACGCCAAAATGCCCAAGCAAGCCAGCATAAATTTTTATATGAAACCAATGTAGGTGCGGGCTTGC
CAGTTATTGAAAATCTCCAAAACTTACTTGCAGCAGGTGATGAGCTTGAATATTTTGAAGGGATTTTATCTGGCTCGCTT
TCCTTTATTTTTGGTAAGTTGGAAGAAGGACTTTCTCTTTCAGAAGTAACCGCACTTGCGCGTGAAAAAGGCTTTACTGA
GCCAGATCCTCGAGATGATCTTTCTGGACAAGATGTAGCACGCAAATTATTAATTCTTGCTCGTGAAGCGGGAATTGAAC
TTGAACTTTCAGATGTTGAAGTTGAAGGTGTATTGCCGAAAGGCTTCTCCGATGGAAAATCTGCAGATGAGTTTATGGCA
ATGTTGCCACAGCTTGACGAGGAGTTTAAAACTCGCGTTGCCACAGCTAAAGCAGAAGGCAAAGTATTGCGTTATGTTGG
AAAAATTAGTGAAGGAAAATGCAAAGTATCCATCGTTGCGGTAGATCTGAATAATCCACTTTATAAAGTGAAAGATGGTG
AAAATGCCTTGGCATTTTATACTCGTTATTATCAACCGATTCCACTTTTATTACGTGGTTATGGCGCAGGTAATGCAGTT
ACTGCGGCAGGTATCTTTGCAGATATTTTAAGAACATTACAACATTAA

Upstream 100 bases:

>100_bases
CGGGTTAGTGCAGCAAAAACAAGATACAGAAAACCCGCGATTCAACTGAATAGCGGGTTTTTTTATAAATTTAAAAACAC
AATATCTAGGAGAACCAATT

Downstream 100 bases:

>100_bases
TAAGGATTCCCAATGTTAAGAATTTACGCTCCTGCATCTAGCGCGAATATTAGCGTAGGTTTCGATACTTTAGGTGCTGC
GATCTCTCCTATTGATGGTT

Product: bifunctional aspartokinase I/homoserine dehydrogenase I

Products: NA

Alternate protein names: AK-HD; Aspartokinase; Homoserine dehydrogenase [H]

Number of amino acids: Translated: 815; Mature: 815

Protein sequence:

>815_residues
MRVLKFGGTSLANPERFSQAAKLIEQAHLEEQAAGVLSAPAKITNHLVALSEKAALNQSTDTHFNEAIEIFYNIINGLHA
ENNQFDLNGTKALIDAEFAQIKGLLEEIRQAGKVEDAVKATIDCRGEKLSIAMMKAWFEARGYSVHIVDPVKQLLAKGGY
LESSVEIEESTKRVDAASIAKDKVVLMAGFTAGNEKGELVLLGRNGSDYSAACLAACLGASVCEIWTDVDGVYTCDPRLV
PDARLLPTLSYREAMELSYFGAKVIHPRTIGPLLPQNIPCVIKNTGNPSAPGSIIDGNVKSESLQVKGITNLDNLAMFNV
SGPGMQGMVGMASRVFSAMSGAGISVILITQSSSEYSISFCVPVKSAEVAKTVLETEFANELNEHQLESIEVIKDLSIIS
VVGDGMKQAKGIAARFFSALAQANISIVAIAQGSSERSISAVVPQNKAIEAVKATHQALFNNKKVVDMFLVGVGGVGGEL
IEQVKRQKEYLAKKNVEIRVCAIANSNRMLLDENGLNLEDWKNDLENATQPSDFDVLLSFIKLHHVVNPVFVDCTSAESV
AGLYARALKEGFHVVTPNKKANTRELVYYNELRQNAQASQHKFLYETNVGAGLPVIENLQNLLAAGDELEYFEGILSGSL
SFIFGKLEEGLSLSEVTALAREKGFTEPDPRDDLSGQDVARKLLILAREAGIELELSDVEVEGVLPKGFSDGKSADEFMA
MLPQLDEEFKTRVATAKAEGKVLRYVGKISEGKCKVSIVAVDLNNPLYKVKDGENALAFYTRYYQPIPLLLRGYGAGNAV
TAAGIFADILRTLQH

Sequences:

>Translated_815_residues
MRVLKFGGTSLANPERFSQAAKLIEQAHLEEQAAGVLSAPAKITNHLVALSEKAALNQSTDTHFNEAIEIFYNIINGLHA
ENNQFDLNGTKALIDAEFAQIKGLLEEIRQAGKVEDAVKATIDCRGEKLSIAMMKAWFEARGYSVHIVDPVKQLLAKGGY
LESSVEIEESTKRVDAASIAKDKVVLMAGFTAGNEKGELVLLGRNGSDYSAACLAACLGASVCEIWTDVDGVYTCDPRLV
PDARLLPTLSYREAMELSYFGAKVIHPRTIGPLLPQNIPCVIKNTGNPSAPGSIIDGNVKSESLQVKGITNLDNLAMFNV
SGPGMQGMVGMASRVFSAMSGAGISVILITQSSSEYSISFCVPVKSAEVAKTVLETEFANELNEHQLESIEVIKDLSIIS
VVGDGMKQAKGIAARFFSALAQANISIVAIAQGSSERSISAVVPQNKAIEAVKATHQALFNNKKVVDMFLVGVGGVGGEL
IEQVKRQKEYLAKKNVEIRVCAIANSNRMLLDENGLNLEDWKNDLENATQPSDFDVLLSFIKLHHVVNPVFVDCTSAESV
AGLYARALKEGFHVVTPNKKANTRELVYYNELRQNAQASQHKFLYETNVGAGLPVIENLQNLLAAGDELEYFEGILSGSL
SFIFGKLEEGLSLSEVTALAREKGFTEPDPRDDLSGQDVARKLLILAREAGIELELSDVEVEGVLPKGFSDGKSADEFMA
MLPQLDEEFKTRVATAKAEGKVLRYVGKISEGKCKVSIVAVDLNNPLYKVKDGENALAFYTRYYQPIPLLLRGYGAGNAV
TAAGIFADILRTLQH
>Mature_815_residues
MRVLKFGGTSLANPERFSQAAKLIEQAHLEEQAAGVLSAPAKITNHLVALSEKAALNQSTDTHFNEAIEIFYNIINGLHA
ENNQFDLNGTKALIDAEFAQIKGLLEEIRQAGKVEDAVKATIDCRGEKLSIAMMKAWFEARGYSVHIVDPVKQLLAKGGY
LESSVEIEESTKRVDAASIAKDKVVLMAGFTAGNEKGELVLLGRNGSDYSAACLAACLGASVCEIWTDVDGVYTCDPRLV
PDARLLPTLSYREAMELSYFGAKVIHPRTIGPLLPQNIPCVIKNTGNPSAPGSIIDGNVKSESLQVKGITNLDNLAMFNV
SGPGMQGMVGMASRVFSAMSGAGISVILITQSSSEYSISFCVPVKSAEVAKTVLETEFANELNEHQLESIEVIKDLSIIS
VVGDGMKQAKGIAARFFSALAQANISIVAIAQGSSERSISAVVPQNKAIEAVKATHQALFNNKKVVDMFLVGVGGVGGEL
IEQVKRQKEYLAKKNVEIRVCAIANSNRMLLDENGLNLEDWKNDLENATQPSDFDVLLSFIKLHHVVNPVFVDCTSAESV
AGLYARALKEGFHVVTPNKKANTRELVYYNELRQNAQASQHKFLYETNVGAGLPVIENLQNLLAAGDELEYFEGILSGSL
SFIFGKLEEGLSLSEVTALAREKGFTEPDPRDDLSGQDVARKLLILAREAGIELELSDVEVEGVLPKGFSDGKSADEFMA
MLPQLDEEFKTRVATAKAEGKVLRYVGKISEGKCKVSIVAVDLNNPLYKVKDGENALAFYTRYYQPIPLLLRGYGAGNAV
TAAGIFADILRTLQH

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ACT domains [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=814, Percent_Identity=62.4078624078624, Blast_Score=1009, Evalue=0.0,
Organism=Escherichia coli, GI1790376, Length=818, Percent_Identity=28.9731051344743, Blast_Score=325, Evalue=7e-90,
Organism=Escherichia coli, GI1790455, Length=467, Percent_Identity=28.2655246252677, Blast_Score=138, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6322599, Length=338, Percent_Identity=35.5029585798817, Blast_Score=186, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6320893, Length=465, Percent_Identity=29.6774193548387, Blast_Score=159, Evalue=2e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR005106
- InterPro:   IPR001341
- InterPro:   IPR018042
- InterPro:   IPR011147
- InterPro:   IPR001342
- InterPro:   IPR019811
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT; PF00742 Homoserine_dh; PF03447 NAD_binding_3 [H]

EC number: =2.7.2.4; =1.1.1.3 [H]

Molecular weight: Translated: 88127; Mature: 88127

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS01042 HOMOSER_DHGENASE ; PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVLKFGGTSLANPERFSQAAKLIEQAHLEEQAAGVLSAPAKITNHLVALSEKAALNQST
CEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCC
DTHFNEAIEIFYNIINGLHAENNQFDLNGTKALIDAEFAQIKGLLEEIRQAGKVEDAVKA
CHHHHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
TIDCRGEKLSIAMMKAWFEARGYSVHIVDPVKQLLAKGGYLESSVEIEESTKRVDAASIA
HHCCCCCEEHHHHHHHHHHHCCCEEEEHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHC
KDKVVLMAGFTAGNEKGELVLLGRNGSDYSAACLAACLGASVCEIWTDVDGVYTCDPRLV
CCCEEEEEEEECCCCCCCEEEEECCCCCHHHHHHHHHHCCHHHHHHHCCCCEEECCCCCC
PDARLLPTLSYREAMELSYFGAKVIHPRTIGPLLPQNIPCVIKNTGNPSAPGSIIDGNVK
CCCHHCCCCHHHHHHHHHHCCCEEECCCCCCCCCCCCCCEEEECCCCCCCCCCEEECCCC
SESLQVKGITNLDNLAMFNVSGPGMQGMVGMASRVFSAMSGAGISVILITQSSSEYSISF
CCCEEEEECCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCEEEEE
CVPVKSAEVAKTVLETEFANELNEHQLESIEVIKDLSIISVVGDGMKQAKGIAARFFSAL
EEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
AQANISIVAIAQGSSERSISAVVPQNKAIEAVKATHQALFNNKKVVDMFLVGVGGVGGEL
HHCCEEEEEEECCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHH
IEQVKRQKEYLAKKNVEIRVCAIANSNRMLLDENGLNLEDWKNDLENATQPSDFDVLLSF
HHHHHHHHHHHHHCCCEEEEEEEECCCEEEEECCCCCHHHHHHHHHCCCCCCHHHHHHHH
IKLHHVVNPVFVDCTSAESVAGLYARALKEGFHVVTPNKKANTRELVYYNELRQNAQASQ
HHHHHHCCCEEEEECCCHHHHHHHHHHHHCCCEEECCCCCCCCEEEEEHHHHHHHHHHHH
HKFLYETNVGAGLPVIENLQNLLAAGDELEYFEGILSGSLSFIFGKLEEGLSLSEVTALA
CCEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHH
REKGFTEPDPRDDLSGQDVARKLLILAREAGIELELSDVEVEGVLPKGFSDGKSADEFMA
HHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECEEEEECCCCCCCCCCCHHHHHH
MLPQLDEEFKTRVATAKAEGKVLRYVGKISEGKCKVSIVAVDLNNPLYKVKDGENALAFY
HHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCEEEEEEEEECCCCEEEEECCCCHHHHH
TRYYQPIPLLLRGYGAGNAVTAAGIFADILRTLQH
HHHCCCCCCEEECCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRVLKFGGTSLANPERFSQAAKLIEQAHLEEQAAGVLSAPAKITNHLVALSEKAALNQST
CEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCC
DTHFNEAIEIFYNIINGLHAENNQFDLNGTKALIDAEFAQIKGLLEEIRQAGKVEDAVKA
CHHHHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
TIDCRGEKLSIAMMKAWFEARGYSVHIVDPVKQLLAKGGYLESSVEIEESTKRVDAASIA
HHCCCCCEEHHHHHHHHHHHCCCEEEEHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHC
KDKVVLMAGFTAGNEKGELVLLGRNGSDYSAACLAACLGASVCEIWTDVDGVYTCDPRLV
CCCEEEEEEEECCCCCCCEEEEECCCCCHHHHHHHHHHCCHHHHHHHCCCCEEECCCCCC
PDARLLPTLSYREAMELSYFGAKVIHPRTIGPLLPQNIPCVIKNTGNPSAPGSIIDGNVK
CCCHHCCCCHHHHHHHHHHCCCEEECCCCCCCCCCCCCCEEEECCCCCCCCCCEEECCCC
SESLQVKGITNLDNLAMFNVSGPGMQGMVGMASRVFSAMSGAGISVILITQSSSEYSISF
CCCEEEEECCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCEEEEE
CVPVKSAEVAKTVLETEFANELNEHQLESIEVIKDLSIISVVGDGMKQAKGIAARFFSAL
EEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
AQANISIVAIAQGSSERSISAVVPQNKAIEAVKATHQALFNNKKVVDMFLVGVGGVGGEL
HHCCEEEEEEECCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHH
IEQVKRQKEYLAKKNVEIRVCAIANSNRMLLDENGLNLEDWKNDLENATQPSDFDVLLSF
HHHHHHHHHHHHHCCCEEEEEEEECCCEEEEECCCCCHHHHHHHHHCCCCCCHHHHHHHH
IKLHHVVNPVFVDCTSAESVAGLYARALKEGFHVVTPNKKANTRELVYYNELRQNAQASQ
HHHHHHCCCEEEEECCCHHHHHHHHHHHHCCCEEECCCCCCCCEEEEEHHHHHHHHHHHH
HKFLYETNVGAGLPVIENLQNLLAAGDELEYFEGILSGSLSFIFGKLEEGLSLSEVTALA
CCEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHH
REKGFTEPDPRDDLSGQDVARKLLILAREAGIELELSDVEVEGVLPKGFSDGKSADEFMA
HHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECEEEEECCCCCCCCCCCHHHHHH
MLPQLDEEFKTRVATAKAEGKVLRYVGKISEGKCKVSIVAVDLNNPLYKVKDGENALAFY
HHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCEEEEEEEEECCCCEEEEECCCCHHHHH
TRYYQPIPLLLRGYGAGNAVTAAGIFADILRTLQH
HHHCCCCCCEEECCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]