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The map label for this gene is dapE [H]

Identifier: 148825625

GI number: 148825625

Start: 560755

End: 561888

Strand: Direct

Name: dapE [H]

Synonym: CGSHiEE_02785

Alternate gene names: 148825625

Gene position: 560755-561888 (Clockwise)

Preceding gene: 148825624

Following gene: 148825626

Centisome position: 30.93

GC content: 41.36

Gene sequence:

>1134_bases
ATGAAAGAAAAAGTGGTTTCGTTGGCGCAAGATTTAATTCGTCGCCCGTCTATTAGCCCAAATGACGAAGGCTGTCAGCA
AATTATTGCCGAAGGTTTAGAAAAATTAGGTTTTCAAATTGAATGGATGCCTTTTAATGACACGTTGAATTTGTGGGCAA
AACACGGAACGAGCGAGCCAGTTATTGCGTTTGTAGGACATACGGATGTTGTGCCTACTGGCGATGAAAATCAGTGGTCG
TCTCCGCCGTTTTCTGCGGAGATTATTGATGGCATACTTTATGGGCGTGGTGCGGCGGATATGAAAGGCTCCCTTGCAGC
AATGATTGTGGCGGCAGAAGAATACGTAAAAGCAAATCCTAACCATAAAGGCACCATTGCATTATTGATTACCTCTGATG
AAGAGGCTGCTGCAAAAGATGGAACTATACGCGTGGTTGAGACGTTAATGACGCGTGATGAAAAAATTACTTATTGTATG
GTTGGCGAGCCATCGAGTGCTAAAAACTTAGGCGATGTTGTCAAAAATGGTCGCCGCGGCTCAATTACGGGAAACCTTTA
TATTCAAGGTATTCAAGGACACGTGGCCTATCCGCATTTAGCTGAAAATCCAATCCATAAAGCCGCCCCGTTTTTGCAAG
AATTAACGACCTATCAATGGGATAAAGGTAATGAATTTTTTCCGCCAACAAGCCTACAAATTGCTAACATTCACGCAGGT
ACGGGAAGTAATAATGTAATTCCTGCTGAGTTATATATTCAGTTCAATTTGCGTTATTGCACAGAAGTCACGGATGAAAT
CATTAAGCAAAAAGTGGCTGAAATGCTAGCAAAACATAATTTGAAATATCGTATTGAATGGAATTTATCAGGCAAACCCT
TTTTAACAAAACCAGGTAAATTATTAGATTCGATAACCTCCGCTATTGAGGAAATCACAGGCATAACGCCAAAGGCTGAA
ACAGGTGGTGGCACGTCAGACGGTCGTTTTATTGCATTGATGGGCGCAGAAGTAGTGGAATTTGGCCCGTTAAATTCAAG
CATTCATAAAGTCAATGAATGCGTCAGTGTAGAAGACCTTGGCAAGTGCGGTGAAATTTATCACAAAATGTTAGTGAATT
TATTGGATAGCTAA

Upstream 100 bases:

>100_bases
CCCAATTATTTTACAAGATGGGAAGGCATTAATTGGATTTAATGAAAAGGAATATCAGGCTGCTTTTGCTTAAAGTGCGG
TTATTTTCTTAGGAGTTTTT

Downstream 100 bases:

>100_bases
AATGAAATTAACCCCAGAAATGCTTACAGGAAAGTCTCGTGAGCATTTAGTCAATTTACCCGCAATTCATTCCTCAAATC
ATTTTTTGCAAACGCAAGCT

Product: succinyl-diaminopimelate desuccinylase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase [H]

Number of amino acids: Translated: 377; Mature: 377

Protein sequence:

>377_residues
MKEKVVSLAQDLIRRPSISPNDEGCQQIIAEGLEKLGFQIEWMPFNDTLNLWAKHGTSEPVIAFVGHTDVVPTGDENQWS
SPPFSAEIIDGILYGRGAADMKGSLAAMIVAAEEYVKANPNHKGTIALLITSDEEAAAKDGTIRVVETLMTRDEKITYCM
VGEPSSAKNLGDVVKNGRRGSITGNLYIQGIQGHVAYPHLAENPIHKAAPFLQELTTYQWDKGNEFFPPTSLQIANIHAG
TGSNNVIPAELYIQFNLRYCTEVTDEIIKQKVAEMLAKHNLKYRIEWNLSGKPFLTKPGKLLDSITSAIEEITGITPKAE
TGGGTSDGRFIALMGAEVVEFGPLNSSIHKVNECVSVEDLGKCGEIYHKMLVNLLDS

Sequences:

>Translated_377_residues
MKEKVVSLAQDLIRRPSISPNDEGCQQIIAEGLEKLGFQIEWMPFNDTLNLWAKHGTSEPVIAFVGHTDVVPTGDENQWS
SPPFSAEIIDGILYGRGAADMKGSLAAMIVAAEEYVKANPNHKGTIALLITSDEEAAAKDGTIRVVETLMTRDEKITYCM
VGEPSSAKNLGDVVKNGRRGSITGNLYIQGIQGHVAYPHLAENPIHKAAPFLQELTTYQWDKGNEFFPPTSLQIANIHAG
TGSNNVIPAELYIQFNLRYCTEVTDEIIKQKVAEMLAKHNLKYRIEWNLSGKPFLTKPGKLLDSITSAIEEITGITPKAE
TGGGTSDGRFIALMGAEVVEFGPLNSSIHKVNECVSVEDLGKCGEIYHKMLVNLLDS
>Mature_377_residues
MKEKVVSLAQDLIRRPSISPNDEGCQQIIAEGLEKLGFQIEWMPFNDTLNLWAKHGTSEPVIAFVGHTDVVPTGDENQWS
SPPFSAEIIDGILYGRGAADMKGSLAAMIVAAEEYVKANPNHKGTIALLITSDEEAAAKDGTIRVVETLMTRDEKITYCM
VGEPSSAKNLGDVVKNGRRGSITGNLYIQGIQGHVAYPHLAENPIHKAAPFLQELTTYQWDKGNEFFPPTSLQIANIHAG
TGSNNVIPAELYIQFNLRYCTEVTDEIIKQKVAEMLAKHNLKYRIEWNLSGKPFLTKPGKLLDSITSAIEEITGITPKAE
TGGGTSDGRFIALMGAEVVEFGPLNSSIHKVNECVSVEDLGKCGEIYHKMLVNLLDS

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily [H]

Homologues:

Organism=Homo sapiens, GI271398239, Length=328, Percent_Identity=24.0853658536585, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1788816, Length=375, Percent_Identity=60.2666666666667, Blast_Score=497, Evalue=1e-142,
Organism=Escherichia coli, GI1790395, Length=267, Percent_Identity=29.5880149812734, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1789236, Length=401, Percent_Identity=23.4413965087282, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17551016, Length=216, Percent_Identity=26.3888888888889, Blast_Score=65, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001261
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 41195; Mature: 41195

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00758 ARGE_DAPE_CPG2_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEKVVSLAQDLIRRPSISPNDEGCQQIIAEGLEKLGFQIEWMPFNDTLNLWAKHGTSEP
CCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHCCCCCC
VIAFVGHTDVVPTGDENQWSSPPFSAEIIDGILYGRGAADMKGSLAAMIVAAEEYVKANP
EEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHEEEHHHHHHCCC
NHKGTIALLITSDEEAAAKDGTIRVVETLMTRDEKITYCMVGEPSSAKNLGDVVKNGRRG
CCCCEEEEEEECCCHHHCCCCHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHCCCCC
SITGNLYIQGIQGHVAYPHLAENPIHKAAPFLQELTTYQWDKGNEFFPPTSLQIANIHAG
CEEEEEEEEEECCCEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECC
TGSNNVIPAELYIQFNLRYCTEVTDEIIKQKVAEMLAKHNLKYRIEWNLSGKPFLTKPGK
CCCCCEEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHH
LLDSITSAIEEITGITPKAETGGGTSDGRFIALMGAEVVEFGPLNSSIHKVNECVSVEDL
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCHHHCCCCCCHHHHHHHHHCCHHHH
GKCGEIYHKMLVNLLDS
HHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKEKVVSLAQDLIRRPSISPNDEGCQQIIAEGLEKLGFQIEWMPFNDTLNLWAKHGTSEP
CCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHCCCCCC
VIAFVGHTDVVPTGDENQWSSPPFSAEIIDGILYGRGAADMKGSLAAMIVAAEEYVKANP
EEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHEEEHHHHHHCCC
NHKGTIALLITSDEEAAAKDGTIRVVETLMTRDEKITYCMVGEPSSAKNLGDVVKNGRRG
CCCCEEEEEEECCCHHHCCCCHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHCCCCC
SITGNLYIQGIQGHVAYPHLAENPIHKAAPFLQELTTYQWDKGNEFFPPTSLQIANIHAG
CEEEEEEEEEECCCEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECC
TGSNNVIPAELYIQFNLRYCTEVTDEIIKQKVAEMLAKHNLKYRIEWNLSGKPFLTKPGK
CCCCCEEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHH
LLDSITSAIEEITGITPKAETGGGTSDGRFIALMGAEVVEFGPLNSSIHKVNECVSVEDL
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCHHHCCCCCCHHHHHHHHHCCHHHH
GKCGEIYHKMLVNLLDS
HHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA