Click here to switch to the map view.

The map label for this gene is apbE [H]

Identifier: 148825558

GI number: 148825558

Start: 472678

End: 473718

Strand: Direct

Name: apbE [H]

Synonym: CGSHiEE_02385

Alternate gene names: 148825558

Gene position: 472678-473718 (Clockwise)

Preceding gene: 148825557

Following gene: 148825559

Centisome position: 26.07

GC content: 38.42

Gene sequence:

>1041_bases
ATGAAAAAATTAATAAGCGGTATCATAGCGGTAGCAATGGCATTAAGTCTTGCTGCTTGTCAAAAAGAAACAAAAGTTAT
CTCTTTAAGCGGTAAAACGATGGGGACAACTTATCATGTTAAATACATTGATGATGGTTCAATAAAAGCAACATCTGAAA
AGACGCATGAAGAAATTGAAGCAATCTTAAAAGATGTGAACGCGAAAATGTCCACTTACAAAAAAGATTCGGAATTAAGC
CGCTTCAATCAAAATACCCAAGTGAACACACCGATTGAGATTTCAGCAGATTTTGCCAAAGTATTAGCTGAAGCGATTCG
TTTAAATAAAGTGACCGAAGGCGCATTGGATGTAACTGTTGGCCCTGTCGTGAATTTATGGGGATTTGGCCCTGAAAAAC
GCCCCGAAAAGCAACCTACACCAGAACAATTAGCTGAACGCCAAGCGTGGGTTGGCATTGATAAAATTACCCTAGATACT
AACAAAGAAAAAGCTACATTAAGTAAAGCACTTCCTCAAGTTTACGTAGATTTATCGTCAATTGCTAAAGGCTTTGGCGT
TGATCAGGTGGCTGAAAAGTTAGAACAATTAAATGCTCAGAATTACATGGTTGAAATCGGCGGTGAAATTCGTGCGAAAG
GAAAAAATATTGAAGGTAAACCTTGGCAGATTGCCATTGAAAAACCAACTACAACAGGCGAAAGAGCGGTTGAAGCTGTC
ATTGGATTAAACAATATGGGAATGGCAAGTTCTGGCGATTACCGTATTTACTTTGAAGAAAATGGCAAACGCTTTGCGCA
CGAAATTGATCCGAAAACAGGTTATCCAATTCAGCATCATTTAGCCTCAATTACGGTACTTGCACCAACCTCAATGACTG
CAGATGGCTTATCTACAGGGTTATTTGTGCTGGGGGAAGACAAGGCGTTAGAAGTGGCTGAGAAAAATAATCTTGCCGTT
TATTTAATCATTAGAACAGATAATGGTTTTGTTACCAAATCATCCTCTGCTTTCAAAAAATTAACAGAAACAAAAGAATA
G

Upstream 100 bases:

>100_bases
ATAAAGTGCGGTCAAAATTTAAGATGTTTTTAAACATTTTCTATGATTACAGGTAAGTTTTGTTGAGTTTGCCAGTAATT
CACAAATTTAGGAAATCCAG

Downstream 100 bases:

>100_bases
TTATGCAAACTTTATTTTTTACTTTAATCGCTTTCGTCGCAATTATATTGTTGATGTCTATCGGCTTTATTATCAAAAAA
CAAAGTTTAAAAGGCAGCTG

Product: lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MKKLISGIIAVAMALSLAACQKETKVISLSGKTMGTTYHVKYIDDGSIKATSEKTHEEIEAILKDVNAKMSTYKKDSELS
RFNQNTQVNTPIEISADFAKVLAEAIRLNKVTEGALDVTVGPVVNLWGFGPEKRPEKQPTPEQLAERQAWVGIDKITLDT
NKEKATLSKALPQVYVDLSSIAKGFGVDQVAEKLEQLNAQNYMVEIGGEIRAKGKNIEGKPWQIAIEKPTTTGERAVEAV
IGLNNMGMASSGDYRIYFEENGKRFAHEIDPKTGYPIQHHLASITVLAPTSMTADGLSTGLFVLGEDKALEVAEKNNLAV
YLIIRTDNGFVTKSSSAFKKLTETKE

Sequences:

>Translated_346_residues
MKKLISGIIAVAMALSLAACQKETKVISLSGKTMGTTYHVKYIDDGSIKATSEKTHEEIEAILKDVNAKMSTYKKDSELS
RFNQNTQVNTPIEISADFAKVLAEAIRLNKVTEGALDVTVGPVVNLWGFGPEKRPEKQPTPEQLAERQAWVGIDKITLDT
NKEKATLSKALPQVYVDLSSIAKGFGVDQVAEKLEQLNAQNYMVEIGGEIRAKGKNIEGKPWQIAIEKPTTTGERAVEAV
IGLNNMGMASSGDYRIYFEENGKRFAHEIDPKTGYPIQHHLASITVLAPTSMTADGLSTGLFVLGEDKALEVAEKNNLAV
YLIIRTDNGFVTKSSSAFKKLTETKE
>Mature_346_residues
MKKLISGIIAVAMALSLAACQKETKVISLSGKTMGTTYHVKYIDDGSIKATSEKTHEEIEAILKDVNAKMSTYKKDSELS
RFNQNTQVNTPIEISADFAKVLAEAIRLNKVTEGALDVTVGPVVNLWGFGPEKRPEKQPTPEQLAERQAWVGIDKITLDT
NKEKATLSKALPQVYVDLSSIAKGFGVDQVAEKLEQLNAQNYMVEIGGEIRAKGKNIEGKPWQIAIEKPTTTGERAVEAV
IGLNNMGMASSGDYRIYFEENGKRFAHEIDPKTGYPIQHHLASITVLAPTSMTADGLSTGLFVLGEDKALEVAEKNNLAV
YLIIRTDNGFVTKSSSAFKKLTETKE

Specific function: Involved in the conversion of aminoimidazole ribotide (AIR), a purine intermediate, to the 4-amino-5-hydroxymethyl-2- methyl pyrimidine (HMP) moiety of thiamine [H]

COG id: COG1477

COG function: function code H; Membrane-associated lipoprotein involved in thiamine biosynthesis

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ApbE family [H]

Homologues:

Organism=Escherichia coli, GI1788543, Length=339, Percent_Identity=41.5929203539823, Blast_Score=273, Evalue=8e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003374 [H]

Pfam domain/function: PF02424 ApbE [H]

EC number: NA

Molecular weight: Translated: 37808; Mature: 37808

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLISGIIAVAMALSLAACQKETKVISLSGKTMGTTYHVKYIDDGSIKATSEKTHEEIE
CHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEECEEEEEEEECCCCEEECCHHHHHHHH
AILKDVNAKMSTYKKDSELSRFNQNTQVNTPIEISADFAKVLAEAIRLNKVTEGALDVTV
HHHHHHCHHHHHHCCHHHHHHHCCCCCCCCCEEECHHHHHHHHHHHHHHHHCCCCEEEEE
GPVVNLWGFGPEKRPEKQPTPEQLAERQAWVGIDKITLDTNKEKATLSKALPQVYVDLSS
CCHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHH
IAKGFGVDQVAEKLEQLNAQNYMVEIGGEIRAKGKNIEGKPWQIAIEKPTTTGERAVEAV
HHCCCCHHHHHHHHHHCCCCCEEEEECCEEEECCCCCCCCCEEEEEECCCCCHHHHHHHH
IGLNNMGMASSGDYRIYFEENGKRFAHEIDPKTGYPIQHHLASITVLAPTSMTADGLSTG
HCCCCCCCCCCCCEEEEEECCCCEEHHCCCCCCCCCHHHHHEEEEEEECCCCCCCCCCCC
LFVLGEDKALEVAEKNNLAVYLIIRTDNGFVTKSSSAFKKLTETKE
EEEEECCCEEEEECCCCEEEEEEEECCCCEEECCHHHHHHHHHCCC
>Mature Secondary Structure
MKKLISGIIAVAMALSLAACQKETKVISLSGKTMGTTYHVKYIDDGSIKATSEKTHEEIE
CHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEECEEEEEEEECCCCEEECCHHHHHHHH
AILKDVNAKMSTYKKDSELSRFNQNTQVNTPIEISADFAKVLAEAIRLNKVTEGALDVTV
HHHHHHCHHHHHHCCHHHHHHHCCCCCCCCCEEECHHHHHHHHHHHHHHHHCCCCEEEEE
GPVVNLWGFGPEKRPEKQPTPEQLAERQAWVGIDKITLDTNKEKATLSKALPQVYVDLSS
CCHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHH
IAKGFGVDQVAEKLEQLNAQNYMVEIGGEIRAKGKNIEGKPWQIAIEKPTTTGERAVEAV
HHCCCCHHHHHHHHHHCCCCCEEEEECCEEEECCCCCCCCCEEEEEECCCCCHHHHHHHH
IGLNNMGMASSGDYRIYFEENGKRFAHEIDPKTGYPIQHHLASITVLAPTSMTADGLSTG
HCCCCCCCCCCCCEEEEEECCCCEEHHCCCCCCCCCHHHHHEEEEEEECCCCCCCCCCCC
LFVLGEDKALEVAEKNNLAVYLIIRTDNGFVTKSSSAFKKLTETKE
EEEEECCCEEEEECCCCEEEEEEEECCCCEEECCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]