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The map label for this gene is metJ [H]

Identifier: 148825427

GI number: 148825427

Start: 295174

End: 295491

Strand: Direct

Name: metJ [H]

Synonym: CGSHiEE_01585

Alternate gene names: 148825427

Gene position: 295174-295491 (Clockwise)

Preceding gene: 148825425

Following gene: 148825429

Centisome position: 16.28

GC content: 39.62

Gene sequence:

>318_bases
ATGGCGAATTGGGACGGAAAATATATCAGCCCTTATGCAGAACACGGCAAAAAAAGTGAGCAAGTAAAAAAAATTACAGT
ATCTATTCCCATTAAAGTACTGGAAATTTTGACGAATGAACGCACTCGCCGCCAATTAAAAAGTTTACGTCACGCAACTA
ACAGTGAATTGCTTTGTGAAGCATTCTTACACGCATTCACAGGACAACCTTTACCCACCGATGCAGATTTAATGAAAGAG
CGTAATGATGAAATCCCTGAAGATGCCAAAGTGCTTATGCGTGAATTAGGTGTCGATCCAGAAAGCTGGGAATATTAA

Upstream 100 bases:

>100_bases
ACTTATTTTGGAAGTTTAGCCGTCTAGATTGTTATTTATTTGATTTTTAATTAGAATACGGACAATTTGTAAGGTTATCA
ATAAGCACTTAAGGGAAATT

Downstream 100 bases:

>100_bases
CCGCACTTTTCACTTATCCGTTCAAGCCATTCAAAATTAAACATTCAGGGTTGTCATTGCCCTGACAACTGTCGTGCCAG
TGCTGCAATTTTTGTACCAT

Product: transcriptional repressor protein MetJ

Products: NA

Alternate protein names: Met regulon regulatory protein metJ [H]

Number of amino acids: Translated: 105; Mature: 104

Protein sequence:

>105_residues
MANWDGKYISPYAEHGKKSEQVKKITVSIPIKVLEILTNERTRRQLKSLRHATNSELLCEAFLHAFTGQPLPTDADLMKE
RNDEIPEDAKVLMRELGVDPESWEY

Sequences:

>Translated_105_residues
MANWDGKYISPYAEHGKKSEQVKKITVSIPIKVLEILTNERTRRQLKSLRHATNSELLCEAFLHAFTGQPLPTDADLMKE
RNDEIPEDAKVLMRELGVDPESWEY
>Mature_104_residues
ANWDGKYISPYAEHGKKSEQVKKITVSIPIKVLEILTNERTRRQLKSLRHATNSELLCEAFLHAFTGQPLPTDADLMKER
NDEIPEDAKVLMRELGVDPESWEY

Specific function: This regulatory protein, when combined with SAM (S- adenosylmethionine) represses the expression of the methionine regulon and of enzymes involved in SAM synthesis [H]

COG id: COG3060

COG function: function code KE; Transcriptional regulator of met regulon

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metJ family [H]

Homologues:

Organism=Escherichia coli, GI1790373, Length=105, Percent_Identity=81.9047619047619, Blast_Score=187, Evalue=2e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002084
- InterPro:   IPR010985
- ProDom:   PD020365 [H]

Pfam domain/function: PF01340 MetJ [H]

EC number: NA

Molecular weight: Translated: 12111; Mature: 11980

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANWDGKYISPYAEHGKKSEQVKKITVSIPIKVLEILTNERTRRQLKSLRHATNSELLCE
CCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCHHHHHH
AFLHAFTGQPLPTDADLMKERNDEIPEDAKVLMRELGVDPESWEY
HHHHHHCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
ANWDGKYISPYAEHGKKSEQVKKITVSIPIKVLEILTNERTRRQLKSLRHATNSELLCE
CCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCHHHHHH
AFLHAFTGQPLPTDADLMKERNDEIPEDAKVLMRELGVDPESWEY
HHHHHHCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA