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The map label for this gene is ruvB [H]

Identifier: 148825409

GI number: 148825409

Start: 278901

End: 279908

Strand: Direct

Name: ruvB [H]

Synonym: CGSHiEE_01495

Alternate gene names: 148825409

Gene position: 278901-279908 (Clockwise)

Preceding gene: 148825408

Following gene: 148825414

Centisome position: 15.38

GC content: 43.15

Gene sequence:

>1008_bases
ATGATTGAAGCAGATAGAATTATTAGCGGGCAAGCTAAGGTTGATGAAGATGTTATTGACCGTGCTATTCGTCCTAAACT
ATTAGCGGATTATGTTGGGCAGCCGCAAGTACGCGAGCAGATGGATATCTTCATCAAGGCAGCCAAACTACGTCAAGATG
CCTTAGATCATTTATTGATTTTTGGCCCTCCTGGTTTGGGAAAAACAACGCTTGCTAATATTGTGGCAAATGAAATGGGC
GTGAATATTCGTACGACGTCAGGGCCTGTGTTAGAAAAAGCGGGCGATTTGGCTGCAATGCTAACCAATCTTGAACCGCA
TGATGTGTTATTTATTGATGAAATCCATCGACTTTCCCCTGCTATTGAAGAAGTCCTTTATCCAGCAATGGAAGACTATC
AGTTGGATATTATGATTGGCGAAGGACCCGCTGCTCGTTCCATTAAATTAGATTTGCCCCCTTTTACTTTGGTTGGCGCG
ACCACTAGAGCGGGTTCTTTGACTTCGCCATTACGTGATCGTTTTGGTATTGTGCAACGTTTGGAGTTTTATTCTGTAGA
AGATTTAACCTCTATCGTGGCACGAAGTGCTGGCTGTTTAAATCTTGAATTAGAACAACAGGCCGCTTTTGAGGTGGCTC
GTCGTTCACGAGGCACACCTCGAATTGCAAACCGTTTATTACGACGCGTGCGTGATTATGCAGATGTACGCAATGGGGGA
GTAATTTCGGTAGATGTGGCAAAACAAGCGCTTTCAATGTTGGATGTAGACGATGCTGGATTTGATTATTTAGATAGAAA
ATTGTTGTCTGCAGTTATTGAGCGTTTTGATGGTGGACCTGTAGGGCTGGATAATCTTGCTGCTGCAATTGGCGAAGAAC
GTGATACCATTGAAGATGTTTTAGAGCCTTATTTGATTCAACAGGGTTTTTTACAAAGAACGCCACGTGGACGTATTGCA
ACATCGAAAACTTATCGCCATTTCGGTCTGCAGAAATTATCAGACTAG

Upstream 100 bases:

>100_bases
CCTGTAGAAGCAGAAAAAATGGTAAAACGCGTTGCTAAACCTGAATTAACCAGTGAACAAGTTATTCGTGAAGCATTAAA
AGCCGCATTGTAGGAAAAAT

Downstream 100 bases:

>100_bases
ATAAATTTAACTACTAAAAAAGCCTTATTGTATTCAATAAGGCTTTTTTAATTACTCACTATAACTTTTCAAATTACTTA
ATTTTGCTAAACCCACTTCA

Product: Holliday junction DNA helicase RuvB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MIEADRIISGQAKVDEDVIDRAIRPKLLADYVGQPQVREQMDIFIKAAKLRQDALDHLLIFGPPGLGKTTLANIVANEMG
VNIRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPAIEEVLYPAMEDYQLDIMIGEGPAARSIKLDLPPFTLVGA
TTRAGSLTSPLRDRFGIVQRLEFYSVEDLTSIVARSAGCLNLELEQQAAFEVARRSRGTPRIANRLLRRVRDYADVRNGG
VISVDVAKQALSMLDVDDAGFDYLDRKLLSAVIERFDGGPVGLDNLAAAIGEERDTIEDVLEPYLIQQGFLQRTPRGRIA
TSKTYRHFGLQKLSD

Sequences:

>Translated_335_residues
MIEADRIISGQAKVDEDVIDRAIRPKLLADYVGQPQVREQMDIFIKAAKLRQDALDHLLIFGPPGLGKTTLANIVANEMG
VNIRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPAIEEVLYPAMEDYQLDIMIGEGPAARSIKLDLPPFTLVGA
TTRAGSLTSPLRDRFGIVQRLEFYSVEDLTSIVARSAGCLNLELEQQAAFEVARRSRGTPRIANRLLRRVRDYADVRNGG
VISVDVAKQALSMLDVDDAGFDYLDRKLLSAVIERFDGGPVGLDNLAAAIGEERDTIEDVLEPYLIQQGFLQRTPRGRIA
TSKTYRHFGLQKLSD
>Mature_335_residues
MIEADRIISGQAKVDEDVIDRAIRPKLLADYVGQPQVREQMDIFIKAAKLRQDALDHLLIFGPPGLGKTTLANIVANEMG
VNIRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPAIEEVLYPAMEDYQLDIMIGEGPAARSIKLDLPPFTLVGA
TTRAGSLTSPLRDRFGIVQRLEFYSVEDLTSIVARSAGCLNLELEQQAAFEVARRSRGTPRIANRLLRRVRDYADVRNGG
VISVDVAKQALSMLDVDDAGFDYLDRKLLSAVIERFDGGPVGLDNLAAAIGEERDTIEDVLEPYLIQQGFLQRTPRGRIA
TSKTYRHFGLQKLSD

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG2255

COG function: function code L; Holliday junction resolvasome, helicase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvB family [H]

Homologues:

Organism=Escherichia coli, GI1788167, Length=330, Percent_Identity=81.8181818181818, Blast_Score=550, Evalue=1e-158,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR004605
- InterPro:   IPR008823
- InterPro:   IPR008824
- InterPro:   IPR011991
- ProDom:   PD005323 [H]

Pfam domain/function: PF05491 RuvB_C; PF05496 RuvB_N [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 36992; Mature: 36992

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEADRIISGQAKVDEDVIDRAIRPKLLADYVGQPQVREQMDIFIKAAKLRQDALDHLLI
CCCCCCCCCCCCCCCHHHHHHHHCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCEEE
FGPPGLGKTTLANIVANEMGVNIRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSP
ECCCCCCHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHH
AIEEVLYPAMEDYQLDIMIGEGPAARSIKLDLPPFTLVGATTRAGSLTSPLRDRFGIVQR
HHHHHHHHCCCCCEEEEEECCCCCCCEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHH
LEFYSVEDLTSIVARSAGCLNLELEQQAAFEVARRSRGTPRIANRLLRRVRDYADVRNGG
HHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCC
VISVDVAKQALSMLDVDDAGFDYLDRKLLSAVIERFDGGPVGLDNLAAAIGEERDTIEDV
EEEHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHH
LEPYLIQQGFLQRTPRGRIATSKTYRHFGLQKLSD
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MIEADRIISGQAKVDEDVIDRAIRPKLLADYVGQPQVREQMDIFIKAAKLRQDALDHLLI
CCCCCCCCCCCCCCCHHHHHHHHCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCEEE
FGPPGLGKTTLANIVANEMGVNIRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSP
ECCCCCCHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHH
AIEEVLYPAMEDYQLDIMIGEGPAARSIKLDLPPFTLVGATTRAGSLTSPLRDRFGIVQR
HHHHHHHHCCCCCEEEEEECCCCCCCEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHH
LEFYSVEDLTSIVARSAGCLNLELEQQAAFEVARRSRGTPRIANRLLRRVRDYADVRNGG
HHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCC
VISVDVAKQALSMLDVDDAGFDYLDRKLLSAVIERFDGGPVGLDNLAAAIGEERDTIEDV
EEEHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHH
LEPYLIQQGFLQRTPRGRIATSKTYRHFGLQKLSD
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA