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The map label for this gene is ruvC [H]

Identifier: 148825407

GI number: 148825407

Start: 277644

End: 278216

Strand: Direct

Name: ruvC [H]

Synonym: CGSHiEE_01485

Alternate gene names: 148825407

Gene position: 277644-278216 (Clockwise)

Preceding gene: 148825406

Following gene: 148825408

Centisome position: 15.31

GC content: 41.54

Gene sequence:

>573_bases
ATGAGCATTATTTTAGGTATTGACCCAGGCTCTCGCGTAACTGGCTACGGTGTGATTCGTCAAACAGGAAGACATTTAGA
ATATCTCGGTAGTGGTGCAATTCGTACTCAAGTTGAAGATTTACCAACCCGTTTGAAACGCATTTATGCTGGAGTAACTG
AAATCATCACGCAATTTCAACCAGATATGTTTGCGATTGAGCAAGTGTTTATGGCTAAGAATGCGGATTCTGCTTTGAAA
CTCGGGCAGGCTCGCGGTACGGCGATTGTTGCTGCAGTAAATCATGATCTACCTGTTTTTGAATATGCCGCACGTTTAGT
AAAACAAACAGTGGTGGGCATTGGTTCCGCAGATAAAGTGCAAGTGCAAGAAATGGTGACTCGTATTTTGAAGTTGTCTG
ATAAACCTCAAACCGATGCTGCGGATGCGTTGGCTATCGCGATTACACATGCGCATTCTATTCAACATTCTTTACATATT
GCCAATTCTGTGAAAATGACAGAAACGCAAGAAAAAATGACCGCACTTTTAAAGACCAGATATAGCCGAGGACGCTTTAG
ATTAAAAATTTAA

Upstream 100 bases:

>100_bases
CAAAACGTATATCACAACGGAGAAATCTGTGACGAAGTGGCATCTCAACTTTAATAATGCAATAAATGGGTAATTTAATT
ACCCATTTTTTATAGAAATT

Downstream 100 bases:

>100_bases
CTGGATGTTCGTCCAGTTTTATTTTATTCTATGCGTTAAGTTTTTTATCATTCGGAAGAATTATGATCGGTCGCTTACAA
GGCATTCTTTTAGAAAAACA

Product: Holliday junction resolvase

Products: NA

Alternate protein names: Holliday junction nuclease ruvC; Holliday junction resolvase ruvC [H]

Number of amino acids: Translated: 190; Mature: 189

Protein sequence:

>190_residues
MSIILGIDPGSRVTGYGVIRQTGRHLEYLGSGAIRTQVEDLPTRLKRIYAGVTEIITQFQPDMFAIEQVFMAKNADSALK
LGQARGTAIVAAVNHDLPVFEYAARLVKQTVVGIGSADKVQVQEMVTRILKLSDKPQTDAADALAIAITHAHSIQHSLHI
ANSVKMTETQEKMTALLKTRYSRGRFRLKI

Sequences:

>Translated_190_residues
MSIILGIDPGSRVTGYGVIRQTGRHLEYLGSGAIRTQVEDLPTRLKRIYAGVTEIITQFQPDMFAIEQVFMAKNADSALK
LGQARGTAIVAAVNHDLPVFEYAARLVKQTVVGIGSADKVQVQEMVTRILKLSDKPQTDAADALAIAITHAHSIQHSLHI
ANSVKMTETQEKMTALLKTRYSRGRFRLKI
>Mature_189_residues
SIILGIDPGSRVTGYGVIRQTGRHLEYLGSGAIRTQVEDLPTRLKRIYAGVTEIITQFQPDMFAIEQVFMAKNADSALKL
GQARGTAIVAAVNHDLPVFEYAARLVKQTVVGIGSADKVQVQEMVTRILKLSDKPQTDAADALAIAITHAHSIQHSLHIA
NSVKMTETQEKMTALLKTRYSRGRFRLKI

Specific function: Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leav

COG id: COG0817

COG function: function code L; Holliday junction resolvasome, endonuclease subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvC family [H]

Homologues:

Organism=Escherichia coli, GI1788170, Length=187, Percent_Identity=72.192513368984, Blast_Score=243, Evalue=6e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012337
- InterPro:   IPR020563
- InterPro:   IPR002176 [H]

Pfam domain/function: PF02075 RuvC [H]

EC number: =3.1.22.4 [H]

Molecular weight: Translated: 20898; Mature: 20766

Theoretical pI: Translated: 10.33; Mature: 10.33

Prosite motif: PS01321 RUVC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIILGIDPGSRVTGYGVIRQTGRHLEYLGSGAIRTQVEDLPTRLKRIYAGVTEIITQFQ
CEEEEECCCCCCCHHHHHHHHHCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PDMFAIEQVFMAKNADSALKLGQARGTAIVAAVNHDLPVFEYAARLVKQTVVGIGSADKV
CCHHHHHHHHHHCCCCHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCHH
QVQEMVTRILKLSDKPQTDAADALAIAITHAHSIQHSLHIANSVKMTETQEKMTALLKTR
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
YSRGRFRLKI
HCCCEEEEEC
>Mature Secondary Structure 
SIILGIDPGSRVTGYGVIRQTGRHLEYLGSGAIRTQVEDLPTRLKRIYAGVTEIITQFQ
EEEEECCCCCCCHHHHHHHHHCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PDMFAIEQVFMAKNADSALKLGQARGTAIVAAVNHDLPVFEYAARLVKQTVVGIGSADKV
CCHHHHHHHHHHCCCCHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCHH
QVQEMVTRILKLSDKPQTDAADALAIAITHAHSIQHSLHIANSVKMTETQEKMTALLKTR
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
YSRGRFRLKI
HCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA