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The map label for this gene is tolB [H]

Identifier: 148825339

GI number: 148825339

Start: 212916

End: 214130

Strand: Direct

Name: tolB [H]

Synonym: CGSHiEE_01115

Alternate gene names: 148825339

Gene position: 212916-214130 (Clockwise)

Preceding gene: 148825338

Following gene: 148825340

Centisome position: 11.74

GC content: 38.52

Gene sequence:

>1215_bases
ATGAAATTACCTATTTATTTAGACTACGCAGCGACCTGTCCAGTCGATGAACGTGTTGCGAAAAAAATGATGGCGTTTCT
AACCATCGATGGCACATTTGGTAATCCAGCCTCTCGCTCACATAAATTTGGTTGGCAAGCAGAAGAAGCTGTTGATATTG
CGCGTAATCAAATCGCTGATCTCATTGGTGCAGATTCTCGTGAAATTGTCTTTACTTCTGGTGCGACAGAATCTGACAAC
CTCGCTATTAAAGGTGCGGCGCATTTCTATCAAACTAAAGGTAAACACATTCTTACCTGTAAAACTGAACATAAAGCAGT
GTTAGATACTTGCCGTCAATTAGAACGAGAAGGTTTTGAAGTCACTTATTTGTCTCCAGAAGCTGATGGTTTAATTGATT
TAGAAAAATTCAAAGCTGCGCTTCGTCCAGATACAATTCTAGCTTCAATTATGCACGCTAATAATGAAATTGGTGTGTTA
CAAGATATTAAAGCTATTGGCGAACTTTGTCGTGCAAATAAAACAATTTTCCATGTGGATGCAACTCAAAGTGTTGGTAA
AGTTGAAATTAATTTGGAAGAATTAGCTGTTGATTTAATGTCGATGTCTAGCCATAAACTTTACGGGCCAAAAGGTGTTG
GCGCATTGTATGTTCGTCGTAAACCTCGCGTACGTTTAGAGGCGATTATTCATGGTGGTGGTCACGAACGTGGTATGCGT
TCAGGTACATTACCTGTTCATCAAATCGTAGGAATGGGAGAGGCATATCGAATTGCAAAAGAAGAAATGGCATCAGAAAT
GCCGCGCTTAAAAGCACTTCGTGATCGTTTATATAATGGCTTAAAAGATATTGAAGAAACTTATGTAAATGGTTCAATGG
AACATCGTTTAGATAGCAATTTAAATATTAGTTTTAACTATGTTGAGGGAGAATCCTTAATGATGGCGTTGCGCGATATT
GCTGTCTCTTCTGGTTCTGCTTGTACATCTGCTAGCTTAGAGCCATCTTATGTATTGCGCGCACTTGGCTTAAATGATGA
ATTGGCGCACAGTTCAATTCGTTTCACATTAGGTCGTTATACTACTGAAGAAGAAATTGATTACACAATTAATTTAATGA
AGGGCGCGGTAGAAAAACTTCGTGCATTATCGCCATTATGGGATATGTTTAAAGAAGGTATTGATTTAAATACCATTGAG
TGGTCAGCTCACTAA

Upstream 100 bases:

>100_bases
CGTGATTTTAATAATTTATTAGTTAATCAATAATAAAGGATAAGCAGTATATTTTTTAATAAAAAAGTGCGGTTATATTT
AGCATAATTTAGGAGTGAAA

Downstream 100 bases:

>100_bases
TATTTAGTTGCCATTATCTGACAATGGCATTCACTAGAATTGAAAAAGGAAAGTTAAAATGGCTTATAGCGAAAAAGTAA
TTGATCATTATGAAAATCCA

Product: cysteine desulfurase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MKLPIYLDYAATCPVDERVAKKMMAFLTIDGTFGNPASRSHKFGWQAEEAVDIARNQIADLIGADSREIVFTSGATESDN
LAIKGAAHFYQTKGKHILTCKTEHKAVLDTCRQLEREGFEVTYLSPEADGLIDLEKFKAALRPDTILASIMHANNEIGVL
QDIKAIGELCRANKTIFHVDATQSVGKVEINLEELAVDLMSMSSHKLYGPKGVGALYVRRKPRVRLEAIIHGGGHERGMR
SGTLPVHQIVGMGEAYRIAKEEMASEMPRLKALRDRLYNGLKDIEETYVNGSMEHRLDSNLNISFNYVEGESLMMALRDI
AVSSGSACTSASLEPSYVLRALGLNDELAHSSIRFTLGRYTTEEEIDYTINLMKGAVEKLRALSPLWDMFKEGIDLNTIE
WSAH

Sequences:

>Translated_404_residues
MKLPIYLDYAATCPVDERVAKKMMAFLTIDGTFGNPASRSHKFGWQAEEAVDIARNQIADLIGADSREIVFTSGATESDN
LAIKGAAHFYQTKGKHILTCKTEHKAVLDTCRQLEREGFEVTYLSPEADGLIDLEKFKAALRPDTILASIMHANNEIGVL
QDIKAIGELCRANKTIFHVDATQSVGKVEINLEELAVDLMSMSSHKLYGPKGVGALYVRRKPRVRLEAIIHGGGHERGMR
SGTLPVHQIVGMGEAYRIAKEEMASEMPRLKALRDRLYNGLKDIEETYVNGSMEHRLDSNLNISFNYVEGESLMMALRDI
AVSSGSACTSASLEPSYVLRALGLNDELAHSSIRFTLGRYTTEEEIDYTINLMKGAVEKLRALSPLWDMFKEGIDLNTIE
WSAH
>Mature_404_residues
MKLPIYLDYAATCPVDERVAKKMMAFLTIDGTFGNPASRSHKFGWQAEEAVDIARNQIADLIGADSREIVFTSGATESDN
LAIKGAAHFYQTKGKHILTCKTEHKAVLDTCRQLEREGFEVTYLSPEADGLIDLEKFKAALRPDTILASIMHANNEIGVL
QDIKAIGELCRANKTIFHVDATQSVGKVEINLEELAVDLMSMSSHKLYGPKGVGALYVRRKPRVRLEAIIHGGGHERGMR
SGTLPVHQIVGMGEAYRIAKEEMASEMPRLKALRDRLYNGLKDIEETYVNGSMEHRLDSNLNISFNYVEGESLMMALRDI
AVSSGSACTSASLEPSYVLRALGLNDELAHSSIRFTLGRYTTEEEIDYTINLMKGAVEKLRALSPLWDMFKEGIDLNTIE
WSAH

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=402, Percent_Identity=55.2238805970149, Blast_Score=489, Evalue=1e-138,
Organism=Homo sapiens, GI156713448, Length=414, Percent_Identity=29.951690821256, Blast_Score=177, Evalue=2e-44,
Organism=Escherichia coli, GI48994898, Length=404, Percent_Identity=78.960396039604, Blast_Score=688, Evalue=0.0,
Organism=Escherichia coli, GI1787970, Length=181, Percent_Identity=29.8342541436464, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1789175, Length=402, Percent_Identity=23.134328358209, Blast_Score=78, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI25143064, Length=403, Percent_Identity=56.575682382134, Blast_Score=474, Evalue=1e-134,
Organism=Caenorhabditis elegans, GI17533177, Length=325, Percent_Identity=32.3076923076923, Blast_Score=147, Evalue=8e-36,
Organism=Saccharomyces cerevisiae, GI6319831, Length=401, Percent_Identity=56.857855361596, Blast_Score=488, Evalue=1e-139,
Organism=Drosophila melanogaster, GI20129463, Length=402, Percent_Identity=55.9701492537313, Blast_Score=471, Evalue=1e-133,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010240
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 44856; Mature: 44856

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLPIYLDYAATCPVDERVAKKMMAFLTIDGTFGNPASRSHKFGWQAEEAVDIARNQIAD
CCCEEEEECCCCCCHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
LIGADSREIVFTSGATESDNLAIKGAAHFYQTKGKHILTCKTEHKAVLDTCRQLEREGFE
HHCCCCCEEEEECCCCCCCCEEEEEHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHCCCE
VTYLSPEADGLIDLEKFKAALRPDTILASIMHANNEIGVLQDIKAIGELCRANKTIFHVD
EEEECCCCCCCEEHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEEEE
ATQSVGKVEINLEELAVDLMSMSSHKLYGPKGVGALYVRRKPRVRLEAIIHGGGHERGMR
CCCCCCEEEEEHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCEEEEEEEECCCCCCCCC
SGTLPVHQIVGMGEAYRIAKEEMASEMPRLKALRDRLYNGLKDIEETYVNGSMEHRLDSN
CCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCC
LNISFNYVEGESLMMALRDIAVSSGSACTSASLEPSYVLRALGLNDELAHSSIRFTLGRY
CCEEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCHHHHHCCEEEEECCC
TTEEEIDYTINLMKGAVEKLRALSPLWDMFKEGIDLNTIEWSAH
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCC
>Mature Secondary Structure
MKLPIYLDYAATCPVDERVAKKMMAFLTIDGTFGNPASRSHKFGWQAEEAVDIARNQIAD
CCCEEEEECCCCCCHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
LIGADSREIVFTSGATESDNLAIKGAAHFYQTKGKHILTCKTEHKAVLDTCRQLEREGFE
HHCCCCCEEEEECCCCCCCCEEEEEHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHCCCE
VTYLSPEADGLIDLEKFKAALRPDTILASIMHANNEIGVLQDIKAIGELCRANKTIFHVD
EEEECCCCCCCEEHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEEEE
ATQSVGKVEINLEELAVDLMSMSSHKLYGPKGVGALYVRRKPRVRLEAIIHGGGHERGMR
CCCCCCEEEEEHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCEEEEEEEECCCCCCCCC
SGTLPVHQIVGMGEAYRIAKEEMASEMPRLKALRDRLYNGLKDIEETYVNGSMEHRLDSN
CCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCC
LNISFNYVEGESLMMALRDIAVSSGSACTSASLEPSYVLRALGLNDELAHSSIRFTLGRY
CCEEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCHHHHHCCEEEEECCC
TTEEEIDYTINLMKGAVEKLRALSPLWDMFKEGIDLNTIEWSAH
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA