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The map label for this gene is tolB [H]

Identifier: 148825336

GI number: 148825336

Start: 209409

End: 210692

Strand: Direct

Name: tolB [H]

Synonym: CGSHiEE_01090

Alternate gene names: 148825336

Gene position: 209409-210692 (Clockwise)

Preceding gene: 148825335

Following gene: 148825337

Centisome position: 11.55

GC content: 41.9

Gene sequence:

>1284_bases
ATGAAATTATTAAAACGTTTAGTGAGCGTATTTGCGATTGTACTTGCTGTTGGAAGCAATGCATTCGCTGGCGATGAAGT
ACGCATTGTCATTGATGAAGGGGTTGATGGTGCGCGCCCTATTGCTGTCGTGCCATTTGTTGGTTCTGCACCAGAAGATA
TTAGCAAAATAGTTGCAGATGATTTACGTAACAGTGGTAAGTTTAATCCTATTGCGGTGTCTCAAATGCCTCAACGCCCA
ACTTCAGCAGCAGAGGTAAATCCTGAGGCTTGGTCGAATATTGGAATTGACGCAATTGTAATTGGACAAGTGGTTCCATC
GGGTAATGGTTATAGTATTACTTATCAATTAATTGATACGGTTGGTGCATCAGGTACGCCAGGGACTGTATTAATGCAAA
ATAGCTATACAGTAACAAATAAATGGTTACGCTATGGCGCGCATACTGTGAGTGATGAAGTTTTTGAAAAATTAACTGCG
ATCCGTGGTGCCTTTAGAACTCGCATCGCTTATGTTGTGCAAAAAAATGGTGGTTCGCAACCTTATGAAGTTCGTGTAGC
AGATTATGATGGCTATAATCAATTTATCGTCAATCGTAGTGCCCAACCAATTATGTCTCCAGCTTGGTCTCCAGACGGCC
AGCGTTTGGCTTATGTATCGTTTGAAAATAAAAAATCACAACTTGTTGTACAGGATTTAAATTTTGGCGCACGTAAAGTA
GTGGCATCTTTTCAAGGGCATAATGGCGCACCAGCCTTTTCGCCAGATGGTTCTCGTTTAGCTTTTGCTTCTTCTCGTGA
TGGTGTTCTGAATATTTACGTTATGGGAGCAAATGGCGGTACACCTACTCAATTGACGAGTGGTGCGGGTAATAATACTG
AACCAGCGTGGTCGCCAGACGGAAATTCAATTTTATTTACATCCGATAGAAGTGGTTCGCCACAAGTTTATCGAATGGAT
GCAAGCGGTGGTAGTGCAACAGTAGTGGGTGGTCGCGGTAGCGCACAAATTAGTGCAGATGGAAAAACACTTGTGATGAT
TAATGGTAACAATAATGTAGTTAAACAAGATCTCACAACGGGCGTTTCAGAGGTACTTAGTACATCTTTTCTAGGCGAAA
GCCCAAGCCTTTCTCCAAATGGAATTATGATTATTTATAGTTCTACACAGGGCTTAGGAAAGGTGCTACAATTGGTTTCT
GCAGATGGTCGCTTTAAGGCGAGCCTTCCAGGAAGTGATGGTCAAGTTAAATTTCCAGCTTGGTCTCCATACTTAACTAA
ATAA

Upstream 100 bases:

>100_bases
CATCAGATGAAATTTATGAAAAATATAAATCACCAATTATTGACTTTGATATTCGATAATTCTTTAGAAAAATTTATTGT
TAAATTTTAAAGGTAACAAA

Downstream 100 bases:

>100_bases
AAAACTCATTTAGGAGAAATCTAATGAACAAATTTGTTAAATCATTATTAGTTGCAGGTTCTGTAGCTGCATTAGCAGCT
TGTAGTTCATCTAACAACGA

Product: translocation protein TolB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MKLLKRLVSVFAIVLAVGSNAFAGDEVRIVIDEGVDGARPIAVVPFVGSAPEDISKIVADDLRNSGKFNPIAVSQMPQRP
TSAAEVNPEAWSNIGIDAIVIGQVVPSGNGYSITYQLIDTVGASGTPGTVLMQNSYTVTNKWLRYGAHTVSDEVFEKLTA
IRGAFRTRIAYVVQKNGGSQPYEVRVADYDGYNQFIVNRSAQPIMSPAWSPDGQRLAYVSFENKKSQLVVQDLNFGARKV
VASFQGHNGAPAFSPDGSRLAFASSRDGVLNIYVMGANGGTPTQLTSGAGNNTEPAWSPDGNSILFTSDRSGSPQVYRMD
ASGGSATVVGGRGSAQISADGKTLVMINGNNNVVKQDLTTGVSEVLSTSFLGESPSLSPNGIMIIYSSTQGLGKVLQLVS
ADGRFKASLPGSDGQVKFPAWSPYLTK

Sequences:

>Translated_427_residues
MKLLKRLVSVFAIVLAVGSNAFAGDEVRIVIDEGVDGARPIAVVPFVGSAPEDISKIVADDLRNSGKFNPIAVSQMPQRP
TSAAEVNPEAWSNIGIDAIVIGQVVPSGNGYSITYQLIDTVGASGTPGTVLMQNSYTVTNKWLRYGAHTVSDEVFEKLTA
IRGAFRTRIAYVVQKNGGSQPYEVRVADYDGYNQFIVNRSAQPIMSPAWSPDGQRLAYVSFENKKSQLVVQDLNFGARKV
VASFQGHNGAPAFSPDGSRLAFASSRDGVLNIYVMGANGGTPTQLTSGAGNNTEPAWSPDGNSILFTSDRSGSPQVYRMD
ASGGSATVVGGRGSAQISADGKTLVMINGNNNVVKQDLTTGVSEVLSTSFLGESPSLSPNGIMIIYSSTQGLGKVLQLVS
ADGRFKASLPGSDGQVKFPAWSPYLTK
>Mature_427_residues
MKLLKRLVSVFAIVLAVGSNAFAGDEVRIVIDEGVDGARPIAVVPFVGSAPEDISKIVADDLRNSGKFNPIAVSQMPQRP
TSAAEVNPEAWSNIGIDAIVIGQVVPSGNGYSITYQLIDTVGASGTPGTVLMQNSYTVTNKWLRYGAHTVSDEVFEKLTA
IRGAFRTRIAYVVQKNGGSQPYEVRVADYDGYNQFIVNRSAQPIMSPAWSPDGQRLAYVSFENKKSQLVVQDLNFGARKV
VASFQGHNGAPAFSPDGSRLAFASSRDGVLNIYVMGANGGTPTQLTSGAGNNTEPAWSPDGNSILFTSDRSGSPQVYRMD
ASGGSATVVGGRGSAQISADGKTLVMINGNNNVVKQDLTTGVSEVLSTSFLGESPSLSPNGIMIIYSSTQGLGKVLQLVS
ADGRFKASLPGSDGQVKFPAWSPYLTK

Specific function: Involved in the tonB-independent uptake of proteins [H]

COG id: COG0823

COG function: function code U; Periplasmic component of the Tol biopolymer transport system

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tolB family [H]

Homologues:

Organism=Escherichia coli, GI1786961, Length=437, Percent_Identity=60.6407322654462, Blast_Score=516, Evalue=1e-148,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011659
- InterPro:   IPR014167
- InterPro:   IPR007195
- InterPro:   IPR015943
- InterPro:   IPR001680 [H]

Pfam domain/function: PF07676 PD40; PF04052 TolB_N [H]

EC number: NA

Molecular weight: Translated: 45056; Mature: 45056

Theoretical pI: Translated: 8.61; Mature: 8.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLLKRLVSVFAIVLAVGSNAFAGDEVRIVIDEGVDGARPIAVVPFVGSAPEDISKIVAD
CHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCEEEEEECCCCHHHHHHHHHH
DLRNSGKFNPIAVSQMPQRPTSAAEVNPEAWSNIGIDAIVIGQVVPSGNGYSITYQLIDT
HHHCCCCCCCEECCCCCCCCCCCCCCCHHHHHCCCCCEEEEEEEEECCCCCEEEEEEEEC
VGASGTPGTVLMQNSYTVTNKWLRYGAHTVSDEVFEKLTAIRGAFRTRIAYVVQKNGGSQ
CCCCCCCCEEEEECCEEHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCC
PYEVRVADYDGYNQFIVNRSAQPIMSPAWSPDGQRLAYVSFENKKSQLVVQDLNFGARKV
CEEEEEEECCCCCEEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEECCCCHHHH
VASFQGHNGAPAFSPDGSRLAFASSRDGVLNIYVMGANGGTPTQLTSGAGNNTEPAWSPD
HHHHCCCCCCCCCCCCCCEEEEECCCCCEEEEEEEECCCCCCCEEECCCCCCCCCCCCCC
GNSILFTSDRSGSPQVYRMDASGGSATVVGGRGSAQISADGKTLVMINGNNNVVKQDLTT
CCEEEEECCCCCCCEEEEEECCCCCEEEECCCCCEEEECCCCEEEEEECCCCEEHHHHHH
GVSEVLSTSFLGESPSLSPNGIMIIYSSTQGLGKVLQLVSADGRFKASLPGSDGQVKFPA
HHHHHHHHHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHCCCCCEEEECCCCCCEEECCC
WSPYLTK
CCCCCCC
>Mature Secondary Structure
MKLLKRLVSVFAIVLAVGSNAFAGDEVRIVIDEGVDGARPIAVVPFVGSAPEDISKIVAD
CHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCEEEEEECCCCHHHHHHHHHH
DLRNSGKFNPIAVSQMPQRPTSAAEVNPEAWSNIGIDAIVIGQVVPSGNGYSITYQLIDT
HHHCCCCCCCEECCCCCCCCCCCCCCCHHHHHCCCCCEEEEEEEEECCCCCEEEEEEEEC
VGASGTPGTVLMQNSYTVTNKWLRYGAHTVSDEVFEKLTAIRGAFRTRIAYVVQKNGGSQ
CCCCCCCCEEEEECCEEHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCC
PYEVRVADYDGYNQFIVNRSAQPIMSPAWSPDGQRLAYVSFENKKSQLVVQDLNFGARKV
CEEEEEEECCCCCEEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEECCCCHHHH
VASFQGHNGAPAFSPDGSRLAFASSRDGVLNIYVMGANGGTPTQLTSGAGNNTEPAWSPD
HHHHCCCCCCCCCCCCCCEEEEECCCCCEEEEEEEECCCCCCCEEECCCCCCCCCCCCCC
GNSILFTSDRSGSPQVYRMDASGGSATVVGGRGSAQISADGKTLVMINGNNNVVKQDLTT
CCEEEEECCCCCCCEEEEEECCCCCEEEECCCCCEEEECCCCEEEEEECCCCEEHHHHHH
GVSEVLSTSFLGESPSLSPNGIMIIYSSTQGLGKVLQLVSADGRFKASLPGSDGQVKFPA
HHHHHHHHHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHCCCCCEEEECCCCCCEEECCC
WSPYLTK
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA