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The map label for this gene is fruK [H]

Identifier: 148825274

GI number: 148825274

Start: 144468

End: 146138

Strand: Direct

Name: fruK [H]

Synonym: CGSHiEE_00760

Alternate gene names: 148825274

Gene position: 144468-146138 (Clockwise)

Preceding gene: 148825273

Following gene: 148825281

Centisome position: 7.97

GC content: 42.01

Gene sequence:

>1671_bases
ATGAAGTTATTTTTAACCCAATCAGCTAATGTAGGCAATGCGAAAGCCTATTTATTACATGAAGTTTTTCGAGCGGCTGC
GCAAAAAGCAAATGTTTCTATTGTAGAAACTGCAGCCGAAGCAGATCTTGTTCTCGTATTTGGTTCTGTCTTACCAAATA
ATCCCGATTTAGTTGGTAAAAAAGTCTTTATTATCGGCGAAGCTATCGCCATGATTTCCCCTGAAGTCACGTTGGCAAAT
GCCTTAGCCAATGGGGCAGATTATGTTGCACCGAAAAGTGCGGTAAGTTTTACAGGTGTTTCTGGTGTAAAAAATATTGT
TGCGGTAACTGCTTGTCCGACAGGCGTTGCGCATACGTTTATGTCTGCTGAAGCGATTGAAGCCTATGCGAAAAAGCAAG
GTTGGAATGTAAAAGTTGAAACCCGTGGTCAAGTTGGGACTGGCAATGAAATTACTGCAGAAGAAGTCGCGGCGGCTGAT
TTGGTATTTATTGCAGCAGATATTGATGTGCCTTTAGATAAATTTAAAGGTAAGCCAATGTACCGCACTTCAACAGGATT
AGCCTTGAAGAAAACCGAGCAAGAATTTGATAAAGCATTTAAAGAGGCCAAAGTTTTTGATGGTGGAAATAATGCGGGAG
TGAAAGAAGAAAGCCGTGAGAAAAAAGGTGTGTATAAACACTTAATGACTGGTGTTTCTCATATGTTACCGTTAGTGGTT
GCGGGGGGATTGTTGATCGCGATTTCCTTTATGTTCAGTTTTAATGTAATTGAAAATACGGGAGTTTTTCAAGATCTTCC
CAATATGTTGATGAATATCGGTAGTGGTGTGGCGTTTAAGTTAATGATCGCCGTATTTGCTGGCTACGTTGCATTTTCTA
TCGCTGATCGTCCAGGGCTTGCAGTTGGTTTGATTGCGGGGATGCTGGCAAGTGAAGCGGGCGCTGGGATTCTTGGTGGT
ATTATTGCAGGTTTCTTAGCGGGTTATGTGGTGAAAGGACTGAATGTGATTATTCGATTGCCAGCAAGCCTGACTTCATT
AAAACCGATTTTAATTTTACCGCTCTTAGGTTCGATGATTGTTGGTTTGACGATGATTTACCTCATCAATCCACCAGTAG
CTGAAATTATGAAAGAGCTAAGTAATTGGCTTACTTCAATGGGCGAAGTGAATGCGATTGTGTTAGGCGCAATTATCGGT
GCAATGATGTGTATCGATATGGGCGGGCCGGTAAATAAAGCAGCATACACCTTTTCTGTTGGATTGATTGCTTCCCAAGT
TTATACGCCAATGGCTGCGGCAATGGCAGCGGGTATGGTTCCACCAATTGGTATGGCAGTTGCGACTTGGATTGCTCGCA
ATAAATTCACAGTTAGCCAACGCGATGCAGGGAAAGCATCATTCGTTCTTGGTTTATGTTTTATTTCTGAAGGTGCATTA
CCATTTGTGGCAGCAGATCCAATTCGAGTCATTATTAGCTCTGTGATTGGTGGTGCCGTGGCTGGTGCGATTTCTATGGG
CTTAAATATTACATTACAAGCTCCGCACGGCGGATTATTTGTCATTCCATTTGTATCTGAGCCGTTGAAATATCTTGGTG
CAATTGCGATTGGTGCTTTATCAACAGGTGTGGTTTATGCCATTATTAAATCGAAAAACAATGCGGAATAA

Upstream 100 bases:

>100_bases
TCCGCTTTTGCTGTGTCTCAAAGTAATGTTGGTGTGAGTGATTTGAGTTTACTTGACCCCATTTTAGAAAAAGTACAAAT
AACGATGATTGAAGGATAGT

Downstream 100 bases:

>100_bases
TACCGCACTTTGAATGTAAAAAATAATTTTCATTGAAAAAGTTAGACTATGAAATCAAGGACTAAGTTCTGTAATCTACA
GGGCTTAGTCCTTTTAGTTA

Product: 1-phosphofructokinase

Products: NA

Alternate protein names: EIIBC-Fru; Fructose-specific phosphotransferase enzyme IIB component; EIII-Fru; PTS system fructose-specific EIIB component; Fructose permease IIC component; PTS system fructose-specific EIIC component [H]

Number of amino acids: Translated: 556; Mature: 556

Protein sequence:

>556_residues
MKLFLTQSANVGNAKAYLLHEVFRAAAQKANVSIVETAAEADLVLVFGSVLPNNPDLVGKKVFIIGEAIAMISPEVTLAN
ALANGADYVAPKSAVSFTGVSGVKNIVAVTACPTGVAHTFMSAEAIEAYAKKQGWNVKVETRGQVGTGNEITAEEVAAAD
LVFIAADIDVPLDKFKGKPMYRTSTGLALKKTEQEFDKAFKEAKVFDGGNNAGVKEESREKKGVYKHLMTGVSHMLPLVV
AGGLLIAISFMFSFNVIENTGVFQDLPNMLMNIGSGVAFKLMIAVFAGYVAFSIADRPGLAVGLIAGMLASEAGAGILGG
IIAGFLAGYVVKGLNVIIRLPASLTSLKPILILPLLGSMIVGLTMIYLINPPVAEIMKELSNWLTSMGEVNAIVLGAIIG
AMMCIDMGGPVNKAAYTFSVGLIASQVYTPMAAAMAAGMVPPIGMAVATWIARNKFTVSQRDAGKASFVLGLCFISEGAL
PFVAADPIRVIISSVIGGAVAGAISMGLNITLQAPHGGLFVIPFVSEPLKYLGAIAIGALSTGVVYAIIKSKNNAE

Sequences:

>Translated_556_residues
MKLFLTQSANVGNAKAYLLHEVFRAAAQKANVSIVETAAEADLVLVFGSVLPNNPDLVGKKVFIIGEAIAMISPEVTLAN
ALANGADYVAPKSAVSFTGVSGVKNIVAVTACPTGVAHTFMSAEAIEAYAKKQGWNVKVETRGQVGTGNEITAEEVAAAD
LVFIAADIDVPLDKFKGKPMYRTSTGLALKKTEQEFDKAFKEAKVFDGGNNAGVKEESREKKGVYKHLMTGVSHMLPLVV
AGGLLIAISFMFSFNVIENTGVFQDLPNMLMNIGSGVAFKLMIAVFAGYVAFSIADRPGLAVGLIAGMLASEAGAGILGG
IIAGFLAGYVVKGLNVIIRLPASLTSLKPILILPLLGSMIVGLTMIYLINPPVAEIMKELSNWLTSMGEVNAIVLGAIIG
AMMCIDMGGPVNKAAYTFSVGLIASQVYTPMAAAMAAGMVPPIGMAVATWIARNKFTVSQRDAGKASFVLGLCFISEGAL
PFVAADPIRVIISSVIGGAVAGAISMGLNITLQAPHGGLFVIPFVSEPLKYLGAIAIGALSTGVVYAIIKSKNNAE
>Mature_556_residues
MKLFLTQSANVGNAKAYLLHEVFRAAAQKANVSIVETAAEADLVLVFGSVLPNNPDLVGKKVFIIGEAIAMISPEVTLAN
ALANGADYVAPKSAVSFTGVSGVKNIVAVTACPTGVAHTFMSAEAIEAYAKKQGWNVKVETRGQVGTGNEITAEEVAAAD
LVFIAADIDVPLDKFKGKPMYRTSTGLALKKTEQEFDKAFKEAKVFDGGNNAGVKEESREKKGVYKHLMTGVSHMLPLVV
AGGLLIAISFMFSFNVIENTGVFQDLPNMLMNIGSGVAFKLMIAVFAGYVAFSIADRPGLAVGLIAGMLASEAGAGILGG
IIAGFLAGYVVKGLNVIIRLPASLTSLKPILILPLLGSMIVGLTMIYLINPPVAEIMKELSNWLTSMGEVNAIVLGAIIG
AMMCIDMGGPVNKAAYTFSVGLIASQVYTPMAAAMAAGMVPPIGMAVATWIARNKFTVSQRDAGKASFVLGLCFISEGAL
PFVAADPIRVIISSVIGGAVAGAISMGLNITLQAPHGGLFVIPFVSEPLKYLGAIAIGALSTGVVYAIIKSKNNAE

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1299

COG function: function code G; Phosphotransferase system, fructose-specific IIC component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1788492, Length=555, Percent_Identity=56.3963963963964, Blast_Score=595, Evalue=1e-171,
Organism=Escherichia coli, GI1786951, Length=435, Percent_Identity=34.7126436781609, Blast_Score=242, Evalue=4e-65,
Organism=Escherichia coli, GI87082348, Length=428, Percent_Identity=31.0747663551402, Blast_Score=224, Evalue=9e-60,
Organism=Escherichia coli, GI1790386, Length=318, Percent_Identity=39.937106918239, Blast_Score=205, Evalue=8e-54,
Organism=Escherichia coli, GI1788729, Length=397, Percent_Identity=28.463476070529, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI1790387, Length=90, Percent_Identity=51.1111111111111, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1790390, Length=65, Percent_Identity=43.0769230769231, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1788730, Length=89, Percent_Identity=37.0786516853933, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013011
- InterPro:   IPR003501
- InterPro:   IPR003352
- InterPro:   IPR013014
- InterPro:   IPR003353
- InterPro:   IPR006327 [H]

Pfam domain/function: PF02378 PTS_EIIC; PF02302 PTS_IIB [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 57765; Mature: 57765

Theoretical pI: Translated: 8.40; Mature: 8.40

Prosite motif: PS51099 PTS_EIIB_TYPE_2 ; PS51104 PTS_EIIC_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLFLTQSANVGNAKAYLLHEVFRAAAQKANVSIVETAAEADLVLVFGSVLPNNPDLVGK
CEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCEEEEEHHHCCCCCCCCCC
KVFIIGEAIAMISPEVTLANALANGADYVAPKSAVSFTGVSGVKNIVAVTACPTGVAHTF
EEEEEEHHHHHHCCCHHHHHHHHCCCCCCCCCCHHEECCHHHHHHEEEEEECCCHHHHHH
MSAEAIEAYAKKQGWNVKVETRGQVGTGNEITAEEVAAADLVFIAADIDVPLDKFKGKPM
HHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHEEEEEEEECCCCHHHHCCCCC
YRTSTGLALKKTEQEFDKAFKEAKVFDGGNNAGVKEESREKKGVYKHLMTGVSHMLPLVV
EECCCCCEEHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AGGLLIAISFMFSFNVIENTGVFQDLPNMLMNIGSGVAFKLMIAVFAGYVAFSIADRPGL
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHEECCCCCH
AVGLIAGMLASEAGAGILGGIIAGFLAGYVVKGLNVIIRLPASLTSLKPILILPLLGSMI
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEEEECCCHHHHCCHHHHHHHHHHHH
VGLTMIYLINPPVAEIMKELSNWLTSMGEVNAIVLGAIIGAMMCIDMGGPVNKAAYTFSV
HHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCHHEEEEH
GLIASQVYTPMAAAMAAGMVPPIGMAVATWIARNKFTVSQRDAGKASFVLGLCFISEGAL
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHCCCCC
PFVAADPIRVIISSVIGGAVAGAISMGLNITLQAPHGGLFVIPFVSEPLKYLGAIAIGAL
CEEECCHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCEEEECCHHHHHHHHHHHHHHHH
STGVVYAIIKSKNNAE
HHCCEEEEEECCCCCC
>Mature Secondary Structure
MKLFLTQSANVGNAKAYLLHEVFRAAAQKANVSIVETAAEADLVLVFGSVLPNNPDLVGK
CEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCEEEEEHHHCCCCCCCCCC
KVFIIGEAIAMISPEVTLANALANGADYVAPKSAVSFTGVSGVKNIVAVTACPTGVAHTF
EEEEEEHHHHHHCCCHHHHHHHHCCCCCCCCCCHHEECCHHHHHHEEEEEECCCHHHHHH
MSAEAIEAYAKKQGWNVKVETRGQVGTGNEITAEEVAAADLVFIAADIDVPLDKFKGKPM
HHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHEEEEEEEECCCCHHHHCCCCC
YRTSTGLALKKTEQEFDKAFKEAKVFDGGNNAGVKEESREKKGVYKHLMTGVSHMLPLVV
EECCCCCEEHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AGGLLIAISFMFSFNVIENTGVFQDLPNMLMNIGSGVAFKLMIAVFAGYVAFSIADRPGL
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHEECCCCCH
AVGLIAGMLASEAGAGILGGIIAGFLAGYVVKGLNVIIRLPASLTSLKPILILPLLGSMI
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEEEECCCHHHHCCHHHHHHHHHHHH
VGLTMIYLINPPVAEIMKELSNWLTSMGEVNAIVLGAIIGAMMCIDMGGPVNKAAYTFSV
HHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCHHEEEEH
GLIASQVYTPMAAAMAAGMVPPIGMAVATWIARNKFTVSQRDAGKASFVLGLCFISEGAL
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHCCCCC
PFVAADPIRVIISSVIGGAVAGAISMGLNITLQAPHGGLFVIPFVSEPLKYLGAIAIGAL
CEEECCHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCEEEECCHHHHHHHHHHHHHHHH
STGVVYAIIKSKNNAE
HHCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]